BR112016028741A2 - composições e métodos para controle de fluxo rápido e dinâmico usando válvulas metabólicas sintéticas - Google Patents
composições e métodos para controle de fluxo rápido e dinâmico usando válvulas metabólicas sintéticasInfo
- Publication number
- BR112016028741A2 BR112016028741A2 BR112016028741A BR112016028741A BR112016028741A2 BR 112016028741 A2 BR112016028741 A2 BR 112016028741A2 BR 112016028741 A BR112016028741 A BR 112016028741A BR 112016028741 A BR112016028741 A BR 112016028741A BR 112016028741 A2 BR112016028741 A2 BR 112016028741A2
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- Brazil
- Prior art keywords
- strains
- metabolism
- compositions
- fast
- methods
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- C12N9/0004—Oxidoreductases (1.)
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- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
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- C12P13/00—Preparation of nitrogen-containing organic compounds
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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- C12P7/00—Preparation of oxygen-containing organic compounds
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- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
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- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
- C12Y103/01009—Enoyl-[acyl-carrier-protein] reductase (NADH) (1.3.1.9)
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- C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
- C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
- C12Y108/01004—Dihydrolipoyl dehydrogenase (1.8.1.4), i.e. lipoamide-dehydrogenase
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- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
- C12Y203/03001—Citrate (Si)-synthase (2.3.3.1)
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- C12Y—ENZYMES
- C12Y206/00—Transferases transferring nitrogenous groups (2.6)
- C12Y206/01—Transaminases (2.6.1)
- C12Y206/01001—Aspartate transaminase (2.6.1.1), i.e. aspartate-aminotransferase
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
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- C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462010574P | 2014-06-11 | 2014-06-11 | |
| PCT/US2015/035306 WO2016053397A2 (en) | 2014-06-11 | 2015-06-11 | Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112016028741A2 true BR112016028741A2 (pt) | 2017-11-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112016028741A BR112016028741A2 (pt) | 2014-06-11 | 2015-06-11 | composições e métodos para controle de fluxo rápido e dinâmico usando válvulas metabólicas sintéticas |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US10662426B2 (https=) |
| EP (2) | EP3155102B1 (https=) |
| JP (2) | JP6730199B2 (https=) |
| AU (2) | AU2015324564B2 (https=) |
| BR (1) | BR112016028741A2 (https=) |
| DK (1) | DK3155102T5 (https=) |
| ES (1) | ES2937642T3 (https=) |
| GB (1) | GB2528177B (https=) |
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Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| JP6730199B2 (ja) * | 2014-06-11 | 2020-07-29 | デューク ユニバーシティ | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| JP7109784B2 (ja) | 2015-10-23 | 2022-08-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子編集のための進化したCas9蛋白質 |
| LT3604527T (lt) | 2016-06-02 | 2021-06-25 | Sigma-Aldrich Co., Llc | Programuojamų, dnr surišančių baltymų panaudojimas tiksliniam genomo modifikavimui pagerinti |
| US20190367930A1 (en) * | 2016-07-29 | 2019-12-05 | Danmarks Tekniske Universitet | Methods for decoupling cell growth from production of biochemicals and recombinant polypeptides |
| KR102547316B1 (ko) | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| EP3526320A1 (en) | 2016-10-14 | 2019-08-21 | President and Fellows of Harvard College | Aav delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| IL296347B2 (en) * | 2017-02-21 | 2024-03-01 | Univ Duke | Compositions and methods for robust dynamic metabolic control |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| JP2020510038A (ja) | 2017-03-09 | 2020-04-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | がんワクチン |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| EP3797160A1 (en) | 2018-05-23 | 2021-03-31 | The Broad Institute Inc. | Base editors and uses thereof |
| US10227576B1 (en) | 2018-06-13 | 2019-03-12 | Caribou Biosciences, Inc. | Engineered cascade components and cascade complexes |
| US12227776B2 (en) | 2018-06-13 | 2025-02-18 | Caribou Biosciences, Inc. | Engineered cascade components and cascade complexes |
| WO2019246488A1 (en) * | 2018-06-21 | 2019-12-26 | Duke University | Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020061385A1 (en) * | 2018-09-19 | 2020-03-26 | Conagen Inc. | Controllable protein degradation via engineering degradation tag variants in corynebacterium host cells |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| JP7657726B2 (ja) | 2019-03-19 | 2025-04-07 | ザ ブロード インスティテュート,インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| US12577572B2 (en) | 2019-10-23 | 2026-03-17 | Duke University | Compositions, systems, and methods for high level expression of recombinant protein |
| MX2022011418A (es) * | 2020-03-16 | 2022-12-15 | Univ Duke | Métodos y composiciones para el silenciamiento de génes basado en crispr tipo i-e mejorado. |
| CA3179180A1 (en) * | 2020-04-03 | 2021-12-02 | Duke University | Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control |
| US20230183757A1 (en) * | 2020-04-03 | 2023-06-15 | Duke University | Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| IL300042A (en) * | 2020-07-24 | 2023-03-01 | Univ Duke | Methods and compositions for the production of acetyl-COA derivatives |
| WO2022226507A1 (en) * | 2021-04-20 | 2022-10-27 | Duke University | Two-stage dynamic control over redox state improves cystolic expression of disulfide containing proteins in e. coli |
| CN113604416B (zh) * | 2021-09-02 | 2023-05-05 | 天津大学 | 产乙偶姻的大肠杆菌工程菌及构建方法与在全细胞催化生产乙偶姻的应用 |
| WO2023183784A2 (en) * | 2022-03-21 | 2023-09-28 | Duke University | Compositions and methods for improved malonyl-coa biosynthesis using 2-stage dynamic metabolic control |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU752105B2 (en) | 1997-12-05 | 2002-09-05 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Novel dna cloning method |
| US6355412B1 (en) | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| DE10012283A1 (de) * | 2000-03-14 | 2001-09-20 | Thomas Schweder | Wirts-Vektor-Systeme zur phosphatregulierten Überproduktion von Polypeptiden in Becillus |
| DK1412513T3 (da) * | 2001-07-30 | 2010-07-12 | Genencor Int | Phosphatbegrænset inducerbar promotor og Bacillus-ekspressiongssystem |
| RU2541785C2 (ru) | 2007-05-09 | 2015-02-20 | ЛАЛЛЕМАНД ХАНГЕРИ ЛИКВИДИТИ МЕНЕДЖМЕНТ ЭлЭлСи | ВЕКТОР ДЛЯ НОКАУТА ГЕНА АЦЕТАТКИНАЗЫ В Clostridium thermocellum, КЛЕТКА-ХОЗЯИН, ГЕНЕТИЧЕСКИ МОДИФИЦИРОВАННЫЙ МИКРООРГАНИЗМ Clostridium thermocellum, СПОСОБ ПОЛУЧЕНИЯ ТАКОГО МИКРООРГАНИЗМА И СПОСОБ ПРЕОБРАЗОВАНИЯ ЛИГНОЦЕЛЛЮЛОЗНОЙ БИОМАССЫ В ЭТАНОЛ. |
| US8163516B2 (en) * | 2008-02-08 | 2012-04-24 | Algenol Biofuels Inc. | Selection of ADH in genetically modified cyanobacteria for the production of ethanol |
| BRPI0918882A2 (pt) | 2008-07-23 | 2019-02-26 | Opx Biotechnologies Inc | métodos, sistemas e composições para tolerância aumentada de microorganismo para produção de ácido 3-hidroxipropiônico (3-hp) |
| US20110146142A1 (en) | 2008-08-18 | 2011-06-23 | Ls9, Inc. | Systems and methods for production of mixed fatty esters |
| WO2010031083A2 (en) | 2008-09-15 | 2010-03-18 | Opx Biotechnologies, Inc. | Methods, systems and compositions related to reduction of conversions of microbially produced 3-hydroxypropionic acid (3-hp) to aldehyde metabolites |
| CA2747492A1 (en) | 2008-12-22 | 2010-07-01 | Mascoma Corporation | Production of ethanol from lignocellulosic biomass |
| WO2010141468A1 (en) * | 2009-06-01 | 2010-12-09 | Way Jeffrey C | Methods and molecules for yield improvement involving metabolic engineering |
| GB2487866A (en) | 2009-09-27 | 2012-08-08 | Opx Biotechnologies Inc | Method for producing 3-Hydroxypropionic acid and other products |
| WO2011063363A2 (en) | 2009-11-20 | 2011-05-26 | Opx Biotechnologies, Inc. | Production of an organic acid and/or related chemicals |
| US8712173B2 (en) | 2010-03-12 | 2014-04-29 | Mediatek Singapore Pte. Ltd. | Methods for processing 2Nx2N block with N being positive integer greater than four under intra-prediction mode and related processing circuits thereof |
| CA2809284C (en) | 2010-08-31 | 2021-02-23 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| US9243280B2 (en) * | 2011-02-23 | 2016-01-26 | University Of Central Florida Research Foundation, Inc. | Cellular depletion of biomolecular targets |
| CN103562398A (zh) * | 2011-03-22 | 2014-02-05 | Opx生物工艺学公司 | 化学产品的微生物生产以及相关的组合物、方法和系统 |
| SG194691A1 (en) | 2011-04-29 | 2013-12-30 | Danisco Us Inc | Production of mevalonate, isoprene, and isoprenoids using genes encodingpolypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities |
| EP2562249A1 (en) | 2011-08-24 | 2013-02-27 | Helmholtz-Zentrum für Infektionsforschung GmbH | Engineered host cells and methods for the production of cis, cis-muconic acid |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| CA2905049A1 (en) | 2013-03-13 | 2014-10-02 | Trustees Of Boston University | Tunable control of protein degradation in synthetic and endogenous bacterial systems |
| EP2843043A1 (en) * | 2013-08-27 | 2015-03-04 | Evonik Industries AG | A method for producing acyl amino acids |
| PL3105313T3 (pl) * | 2014-04-02 | 2020-06-29 | The Regents Of The University Of California Office Of Technology Transfer | Moduł molekularnego zaworu upustowego dla biochemii syntetycznej który utrzymuje równowagę kofaktorów |
| US20170130210A1 (en) * | 2014-06-09 | 2017-05-11 | Massachusetts Institute Of Technology | Dynamic knockdown of central metabolism for redirecting glucose-6-phosphate fluxes |
| JP6730199B2 (ja) * | 2014-06-11 | 2020-07-29 | デューク ユニバーシティ | 合成代謝弁を用いた迅速かつ動的なフラックス制御のための組成物及び方法 |
| IL296347B2 (en) * | 2017-02-21 | 2024-03-01 | Univ Duke | Compositions and methods for robust dynamic metabolic control |
| WO2019246488A1 (en) * | 2018-06-21 | 2019-12-26 | Duke University | Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control |
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