JP2017515469A5 - - Google Patents

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JP2017515469A5
JP2017515469A5 JP2016564093A JP2016564093A JP2017515469A5 JP 2017515469 A5 JP2017515469 A5 JP 2017515469A5 JP 2016564093 A JP2016564093 A JP 2016564093A JP 2016564093 A JP2016564093 A JP 2016564093A JP 2017515469 A5 JP2017515469 A5 JP 2017515469A5
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oligonucleotide
droplets
cells
particles
nucleic acid
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JP6853667B2 (ja
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JP2016564093A 2014-04-21 2015-04-17 核酸をバーコーディングするためのシステムおよび方法 Active JP6853667B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201461982001P 2014-04-21 2014-04-21
US61/982,001 2014-04-21
US201462065348P 2014-10-17 2014-10-17
US62/065,348 2014-10-17
US201462066188P 2014-10-20 2014-10-20
US62/066,188 2014-10-20
US201462072944P 2014-10-30 2014-10-30
US62/072,944 2014-10-30
PCT/US2015/026443 WO2015164212A1 (en) 2014-04-21 2015-04-17 Systems and methods for barcoding nucleic acids

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JP2017515469A JP2017515469A (ja) 2017-06-15
JP2017515469A5 true JP2017515469A5 (OSRAM) 2018-05-31
JP6853667B2 JP6853667B2 (ja) 2021-03-31

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EP (3) EP3134536B1 (OSRAM)
JP (1) JP6853667B2 (OSRAM)
CN (1) CN107075543B (OSRAM)
AU (1) AU2015250034B2 (OSRAM)
CA (1) CA2946144C (OSRAM)
DK (3) DK3456846T3 (OSRAM)
LT (3) LT3299469T (OSRAM)
WO (1) WO2015164212A1 (OSRAM)

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Publication number Priority date Publication date Assignee Title
WO2005023427A1 (en) 2003-09-05 2005-03-17 Stokes Bio Limited A microfluidic analysis system
US9597644B2 (en) * 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
JP2009536313A (ja) 2006-01-11 2009-10-08 レインダンス テクノロジーズ, インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
EP1981624B1 (en) 2006-02-07 2011-09-07 Stokes Bio Limited A liquid bridge system and method
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ, インコーポレイテッド デジタル検体分析
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
LT3305918T (lt) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CN114534806B (zh) 2014-04-10 2024-03-29 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN105392902B (zh) * 2014-06-24 2021-10-29 生物辐射实验室股份有限公司 数字式pcr条码化
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN107873054B (zh) * 2014-09-09 2022-07-12 博德研究所 用于复合单细胞核酸分析的基于微滴的方法和设备
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
AU2016215304B2 (en) 2015-02-04 2022-01-27 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
WO2016137973A1 (en) * 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
CN107614096A (zh) 2015-03-13 2018-01-19 哈佛学院院长及董事 使用扩增测定细胞
WO2016168584A1 (en) * 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
CN108473984A (zh) * 2015-11-04 2018-08-31 阿特雷卡公司 用于分析与单个细胞相关联的核酸的核酸条形码的组合组
KR20200103895A (ko) * 2015-11-10 2020-09-02 일루미나, 인코포레이티드 관성 소적 생성 및 입자 캡슐화
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017100350A1 (en) * 2015-12-07 2017-06-15 Raindance Technologies, Inc. Multiplexing in partitions using microparticles
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
US10633648B2 (en) 2016-02-12 2020-04-28 University Of Washington Combinatorial photo-controlled spatial sequencing and labeling
DK3452591T5 (da) 2016-05-02 2024-09-09 Encodia Inc Makromolekyleanalyse under anvendelse af nukleinsyrekodning
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US20190329209A1 (en) * 2016-06-14 2019-10-31 Mission Bio, Inc. Methods and compositions for emulsification of solid supports in deformable beads
EP3260201A1 (en) 2016-06-21 2017-12-27 Hifibio Microfluidic filtration unit and related microfluidic system
EP3686287A1 (en) 2016-06-28 2020-07-29 Hifibio Method for transcriptome analysis of single cells
CN110114473A (zh) * 2016-11-23 2019-08-09 斯特拉斯堡大学 靶分子的串联条形码添加以便以单实体分辨率对靶分子进行绝对定量
CA3047328A1 (en) * 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3545089B1 (en) * 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3838268B1 (en) 2017-02-24 2023-05-10 The Regents of the University of California Particle-drop structures and methods for making and using the same
US10329557B2 (en) 2017-03-13 2019-06-25 Gigagen, Inc. Systems and methods for massively parallel combinatorial analysis of single cells
LT3375889T (lt) 2017-03-17 2020-06-25 Hifibio Sas Vienos ląstelės analizė
US11584951B2 (en) 2017-05-02 2023-02-21 The University Of Tokyo Method for integrally detecting nondestructive measurement information and genome-related information of one cell
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
WO2018213643A1 (en) 2017-05-18 2018-11-22 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP3631012B1 (en) 2017-05-26 2022-06-08 AbVitro LLC High-throughput polynucleotide library sequencing and transcriptome analysis
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
WO2019036505A1 (en) * 2017-08-14 2019-02-21 Mission Bio, Inc. METHODS AND COMPOSITIONS FOR EVALUATING THE UNIFORMITY OF AN EMULSION
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
WO2019051335A1 (en) 2017-09-07 2019-03-14 Juno Therapeutics, Inc. METHODS OF IDENTIFYING CELLULAR CHARACTERISTICS RELATED TO RESPONSES ASSOCIATED WITH CELL THERAPY
WO2019060685A1 (en) * 2017-09-22 2019-03-28 Bioventures, Llc SYSTEM AND METHOD FOR PHENOTYPE ANALYSIS AND POLYNUCLEOTIDE SEQUENCING OF BIOLOGICAL PARTICLES THROUGH A DETERMINISTIC BARCODE
WO2019060857A1 (en) * 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES
EP4494757A3 (en) * 2017-09-29 2025-03-19 The Regents of The University of California Method of generating monodisperse emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
KR102878365B1 (ko) * 2017-10-09 2025-10-31 주식회사 마크로젠 단일 분자 서열분석 및 핵산서열 특성화를 위한 고유한 분자 식별자
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN114525273B (zh) 2017-10-27 2025-01-28 10X基因组学有限公司 用于样品制备和分析的方法和系统
US11513126B2 (en) 2017-10-31 2022-11-29 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019104337A1 (en) 2017-11-27 2019-05-31 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
WO2019191321A1 (en) * 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
WO2020011998A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
WO2020012004A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112703252B (zh) 2018-08-03 2024-09-10 10X基因组学有限公司 用于最小化条形码交换的方法和系统
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
CN119492871A (zh) * 2018-09-26 2025-02-21 北卡罗来纳-查佩尔山大学 用于改进测定的化合物、组合物和方法
US20210340596A1 (en) * 2018-09-28 2021-11-04 Fluent Biosciences Inc. Target capture and barcoding in monodisperse droplets
US20220010373A1 (en) * 2018-11-07 2022-01-13 The University Of Tokyo Method for detecting genome-related information of cell coexisting with at least one type of test substance
US11512356B2 (en) * 2018-11-08 2022-11-29 Tokitae Llc Systems and methods for particle multiplexing in droplets
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
AU2019398307A1 (en) 2018-12-13 2021-06-10 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
CN109851711B (zh) * 2018-12-29 2020-09-25 苏州绘真生物科技有限公司 可溶性水凝胶微球及其制备方法与在单细胞检测中的应用
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
CN118979095A (zh) 2019-02-12 2024-11-19 10X基因组学有限公司 用于加工核酸分子的方法
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
CN114302966B (zh) * 2019-03-12 2025-03-21 通用测序技术公司 单细胞胞内捕获的方法及其应用
CN114174532A (zh) * 2019-04-05 2022-03-11 德克萨斯大学系统董事会 细胞条形码编码的方法和应用
JP7582676B2 (ja) 2019-04-30 2024-11-13 エンコディア, インコーポレイテッド 分析物を調製するための方法および関連キット
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
CN114729392A (zh) * 2019-09-06 2022-07-08 10X基因组学有限公司 用于对细胞和细胞珠粒进行条形码化的系统和方法
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
CN111172257A (zh) * 2020-01-16 2020-05-19 南方科技大学 一种带编码的凝胶微粒及其制备方法和应用
EP4095256A4 (en) * 2020-01-23 2023-03-15 DGI Tech (Qing Dao) Co., Limited Droplet microfluidics-based single cell sequencing and applications
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
WO2021194699A1 (en) * 2020-03-24 2021-09-30 Cellecta, Inc. Single cell genetic analysis
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3202814A1 (en) * 2020-12-22 2022-06-30 Hifibio Sas Microfluidic methods and systems
KR20230142832A (ko) 2021-01-08 2023-10-11 셀라노메, 인크. 생물학적 샘플을 분석하기 위한 디바이스 및 방법
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20250034640A1 (en) * 2021-04-20 2025-01-30 Simsen Diagnostics Ab Compositions and methods for cell-free nucleic acid isolation
CN113215233A (zh) * 2021-05-15 2021-08-06 墨卓生物科技(浙江)有限公司 一种用于单细胞测序的具有特定寡核苷酸序列的微球
CN117794646A (zh) 2021-06-04 2024-03-29 伊努梅里斯公司 用于单细胞条形码化和测序的组合物、方法和系统
WO2022256612A1 (en) * 2021-06-04 2022-12-08 Enumerix, Inc. Compositions, methods, and systems for single cell barcoding and sequencing
CN113584598A (zh) * 2021-06-17 2021-11-02 南方科技大学 一种单细胞文库的构建方法
KR20240024835A (ko) * 2021-06-24 2024-02-26 일루미나, 인코포레이티드 비이드-기반 핵산의 조합 인덱싱을 위한 방법 및 조성물
EP4392577B1 (en) * 2021-08-24 2025-11-26 Ecole Polytechnique Federale De Lausanne (Epfl) Optimised set of oligonucleotides for bulk rna barcoding and sequencing
CN113889192B (zh) * 2021-09-29 2024-02-27 西安热工研究院有限公司 一种基于深层降噪自编码器的单细胞RNA-seq数据聚类方法
EP4437128A1 (en) * 2021-12-01 2024-10-02 Vilnius University Methods for processing and barcoding nucleic acids
CN114177960B (zh) * 2021-12-15 2023-04-28 天津大学 基于超高频体声波谐振器的微液滴操纵方法以及装置
WO2023139272A1 (en) * 2022-01-24 2023-07-27 Scipio Bioscience Gelation device with piston
EP4536854A1 (en) * 2022-06-07 2025-04-16 Alithea Genomics SA Optimised set of oligonucleotides for bulk rna barcoding and sequencing
WO2024011226A1 (en) * 2022-07-07 2024-01-11 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cell-barcode recorder devices and methods
EP4331724A1 (en) * 2022-08-29 2024-03-06 Institut Curie Method of extracting a bead from a droplet
EP4435114A1 (en) 2023-03-24 2024-09-25 Miltenyi Biotec B.V. & Co. KG Syntheis of highly multiplexed oligo-barcoded fluorescent beads for single cell seq applications

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
CA2521862C (en) 2003-04-10 2012-10-16 President And Fellows Of Harvard College Formation and control of fluidic species
EP2662135A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for mixing droplets in a microchannel
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
EP2129803A4 (en) * 2007-02-27 2010-11-03 Univ Northwestern APPLICATION OF MOLECULES TO NANOPARTICLES
US9029085B2 (en) * 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20090181375A1 (en) * 2008-01-11 2009-07-16 Peter Brian J Method for detection of nucleic acid barcodes
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3150724A1 (en) 2008-12-19 2017-04-05 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US8691509B2 (en) * 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
SG177369A1 (en) 2009-06-26 2012-02-28 Harvard College Fluid injection
AU2010315580B2 (en) 2009-10-27 2014-11-06 President And Fellows Of Harvard College Droplet creation techniques
WO2012003330A2 (en) * 2010-06-30 2012-01-05 Stratos Genomics, Inc Multiplexed identification of nucleic acid sequences
EP2625320B1 (en) * 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
DK2652155T3 (en) * 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
US9150852B2 (en) * 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
EP2735618B1 (en) * 2011-07-19 2017-12-06 Hitachi High-Technologies Corporation Nucleic acid analysis method
CA2861387A1 (en) * 2012-02-02 2013-08-08 Invenra, Inc. High throughput screen for biologically active polypeptides
LT3305918T (lt) * 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
DK2861760T3 (da) * 2012-06-15 2020-03-09 Univ Texas Sekventering med høj kapacitet af flere udskrifter af en enkelt celle
WO2014047561A1 (en) * 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
SG11201605344YA (en) * 2013-12-30 2016-07-28 Atreca Inc Analysis of nucleic acids associated with single cells using nucleic acid barcodes
CN105392902B (zh) * 2014-06-24 2021-10-29 生物辐射实验室股份有限公司 数字式pcr条码化

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