JP2017504039A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2017504039A5 JP2017504039A5 JP2016563249A JP2016563249A JP2017504039A5 JP 2017504039 A5 JP2017504039 A5 JP 2017504039A5 JP 2016563249 A JP2016563249 A JP 2016563249A JP 2016563249 A JP2016563249 A JP 2016563249A JP 2017504039 A5 JP2017504039 A5 JP 2017504039A5
- Authority
- JP
- Japan
- Prior art keywords
- support
- spot
- detection
- individual
- molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 claims 43
- 238000000034 method Methods 0.000 claims 25
- 150000007523 nucleic acids Chemical class 0.000 claims 12
- 102000039446 nucleic acids Human genes 0.000 claims 12
- 108020004707 nucleic acids Proteins 0.000 claims 12
- 230000003287 optical effect Effects 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 239000002245 particle Substances 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 230000005284 excitation Effects 0.000 claims 2
- 239000002184 metal Substances 0.000 claims 2
- 229910052751 metal Inorganic materials 0.000 claims 2
- 230000001293 nucleolytic effect Effects 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 229910052782 aluminium Inorganic materials 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 229910052804 chromium Inorganic materials 0.000 claims 1
- 239000002131 composite material Substances 0.000 claims 1
- 238000006911 enzymatic reaction Methods 0.000 claims 1
- 230000005281 excited state Effects 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 238000001215 fluorescent labelling Methods 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 229910044991 metal oxide Inorganic materials 0.000 claims 1
- 150000004706 metal oxides Chemical class 0.000 claims 1
- 229910052752 metalloid Inorganic materials 0.000 claims 1
- 150000002738 metalloids Chemical class 0.000 claims 1
- 229910052759 nickel Inorganic materials 0.000 claims 1
- 239000004033 plastic Substances 0.000 claims 1
- 239000010453 quartz Substances 0.000 claims 1
- 229910000077 silane Inorganic materials 0.000 claims 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 1
- 229910052709 silver Inorganic materials 0.000 claims 1
- 238000011895 specific detection Methods 0.000 claims 1
- 230000036962 time dependent Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14150807.7 | 2014-01-10 | ||
| EP14150807 | 2014-01-10 | ||
| PCT/EP2015/050066 WO2015104245A1 (en) | 2014-01-10 | 2015-01-05 | Single molecule analysis with high accuracy |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017504039A JP2017504039A (ja) | 2017-02-02 |
| JP2017504039A5 true JP2017504039A5 (enExample) | 2018-02-01 |
| JP6731854B2 JP6731854B2 (ja) | 2020-07-29 |
Family
ID=49917014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016563249A Active JP6731854B2 (ja) | 2014-01-10 | 2015-01-05 | 高精度での単一分子解析 |
Country Status (5)
| Country | Link |
|---|---|
| US (4) | US11091805B2 (enExample) |
| EP (1) | EP3092314B1 (enExample) |
| JP (1) | JP6731854B2 (enExample) |
| CN (1) | CN106460034B (enExample) |
| WO (1) | WO2015104245A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10829816B2 (en) | 2012-11-19 | 2020-11-10 | Apton Biosystems, Inc. | Methods of analyte detection |
| CN106460034B (zh) * | 2014-01-10 | 2021-05-07 | 格诺特斯控股股份公司 | 具有高准确度的单分子分析 |
| EP3112841A1 (en) | 2015-06-30 | 2017-01-04 | Gnothis Holding SA | Single molecule analysis in an electrical field |
| EP3330763A1 (en) * | 2016-12-05 | 2018-06-06 | Gnothis AB | Apparatus for characterizing luminescent entities |
| CN114921537A (zh) * | 2017-03-17 | 2022-08-19 | 雅普顿生物系统公司 | 测序和高分辨率成像 |
| CN108469429B (zh) * | 2018-02-06 | 2020-11-24 | 西安电子科技大学 | 一种双模态拉曼-光学投影断层成像系统 |
| WO2020061237A1 (en) | 2018-09-19 | 2020-03-26 | Apton Biosystems, Inc. | Densely-packed analyte layers and detection methods |
| EP3896146A4 (en) * | 2018-12-12 | 2022-01-19 | GeneMind Biosciences Company Limited | Optical system, method for calibrating optical system, and sequencing system |
| EP4616193A1 (en) | 2022-11-07 | 2025-09-17 | Gnothis Holding AG | Selective-area coated protein resistant surface |
| EP4379378A1 (en) | 2022-11-29 | 2024-06-05 | Gnothis Holding AG | Selective-area coated protein resistant surface |
| EP4623095A1 (en) | 2022-11-21 | 2025-10-01 | Gnothis Holding AG | Multiple molecular binding sites |
| EP4403643A1 (en) | 2023-01-19 | 2024-07-24 | Gnothis Holding AG | Multiple molecular binding sites |
| EP4375644A1 (en) | 2022-11-22 | 2024-05-29 | Gnothis Holding AG | Fingerprint analysis of single molecule events |
| EP4379355A1 (en) | 2022-11-29 | 2024-06-05 | Gnothis Holding AG | High throughput analysis of single molecule events |
| EP4382614A1 (en) | 2022-12-06 | 2024-06-12 | Gnothis Holding AG | High yield support |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0679251T3 (da) | 1993-01-18 | 1999-01-25 | Evotec Biosystems Aktiengesell | Fremgangsmåde og apparat til vurdering af biopolymerers fitness |
| DE10126083A1 (de) | 2001-05-29 | 2002-12-05 | Gnothis Holding Sa Ecublens | Verwendung von optischen Diffraktionselementen in Nachweisverfahren |
| DE10162536A1 (de) | 2001-12-19 | 2003-07-17 | Gnothis Holding Sa Ecublens | Evaneszenz-basierendes Multiplex-Sequenzierungsverfahren |
| DE102004038359A1 (de) | 2004-08-06 | 2006-03-16 | Rudolf Rigler | Paralleles Hochdurchsatz-Einzelmolekül-Sequenzierungsverfahren |
| US7692783B2 (en) * | 2006-02-13 | 2010-04-06 | Pacific Biosciences Of California | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
| US7715001B2 (en) * | 2006-02-13 | 2010-05-11 | Pacific Biosciences Of California, Inc. | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
| JP5337676B2 (ja) * | 2009-06-25 | 2013-11-06 | 株式会社日立ハイテクノロジーズ | 蛍光分析装置および蛍光検出装置 |
| JP5372876B2 (ja) * | 2010-09-10 | 2013-12-18 | 株式会社日立ハイテクノロジーズ | 核酸分析デバイス，核酸分析装置、及び核酸分析方法 |
| WO2013131888A1 (en) | 2012-03-06 | 2013-09-12 | Rudolf Rigler | Cyclic single molecule sequencing process |
| CN106460034B (zh) * | 2014-01-10 | 2021-05-07 | 格诺特斯控股股份公司 | 具有高准确度的单分子分析 |
| EP3112841A1 (en) * | 2015-06-30 | 2017-01-04 | Gnothis Holding SA | Single molecule analysis in an electrical field |
-
2015
- 2015-01-05 CN CN201580008751.0A patent/CN106460034B/zh active Active
- 2015-01-05 JP JP2016563249A patent/JP6731854B2/ja active Active
- 2015-01-05 WO PCT/EP2015/050066 patent/WO2015104245A1/en not_active Ceased
- 2015-01-05 EP EP15700025.8A patent/EP3092314B1/en active Active
- 2015-01-05 US US15/110,779 patent/US11091805B2/en active Active
-
2021
- 2021-07-15 US US17/376,324 patent/US11788140B2/en active Active
-
2023
- 2023-05-22 US US18/200,109 patent/US12305232B2/en active Active
-
2025
- 2025-04-29 US US19/192,606 patent/US20250257397A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2017504039A5 (enExample) | ||
| US10656149B2 (en) | Analyte detection enhancement by targeted immobilization, surface amplification, and pixelated reading and analysis | |
| Bally et al. | Optical microarray biosensing techniques | |
| Wang et al. | Duplex microfluidic SERS detection of pathogen antigens with nanoyeast single-chain variable fragments | |
| KR102378388B1 (ko) | 개선된 분석 방법 | |
| Vareiro et al. | Surface plasmon fluorescence measurements of human chorionic gonadotrophin: role of antibody orientation in obtaining enhanced sensitivity and limit of detection | |
| EP2217928B1 (en) | Alternate labeling strategies for single molecule sequencing | |
| CA2860104C (en) | Device for use in the detection of binding affinities | |
| WO2014144133A1 (en) | Analyte detection enhancement by targeted immobilization, surface amplification, and pixelated reading and analysis | |
| JP6731854B2 (ja) | 高精度での単一分子解析 | |
| CA2884694C (en) | Device for use in the detection of binding affinities | |
| JP6871440B2 (ja) | 生物学的実体におけるコンホメーションのハイスループット分析のためのシステムおよび方法 | |
| JP2010523973A (ja) | バイオセンサのための較正および正規化の方法 | |
| JP2017525958A5 (enExample) | ||
| US7259847B2 (en) | Use of optical diffraction elements in detection methods | |
| JP2004530137A5 (enExample) | ||
| WO2012144528A1 (ja) | 標的粒子の定量方法、光分析装置及び光分析用コンピュータプログラム | |
| Nava et al. | Label-free optical biosensors in the pandemic era | |
| JP2013511713A (ja) | 向上した蛍光検出及び方法 | |
| US20210010920A1 (en) | Spectroscopic analysis device, spectroscopic analysis method, program, recording medium, and microscope | |
| JP2018526979A (ja) | 電場における単一分子の分析 | |
| JP5663008B2 (ja) | 核酸分析デバイスの製造方法 | |
| TWI487910B (zh) | 倒多角錐陣列型免標定病毒檢測基板、系統及方法 | |
| US20130157264A1 (en) | Nucleic acid analysis device, nucleic acid analysis apparatus, and nucleic acid analysis method | |
| CN103868902A (zh) | 一种生物荧光检测探头 |