CN106460034B - 具有高准确度的单分子分析 - Google Patents

具有高准确度的单分子分析 Download PDF

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Publication number
CN106460034B
CN106460034B CN201580008751.0A CN201580008751A CN106460034B CN 106460034 B CN106460034 B CN 106460034B CN 201580008751 A CN201580008751 A CN 201580008751A CN 106460034 B CN106460034 B CN 106460034B
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nucleic acid
spot
molecule
diameter
detection
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Chinese (zh)
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CN106460034A (zh
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R·里吉勒
L·埃德曼
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Gnothis Holding SA
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Gnothis Holding SA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201580008751.0A 2014-01-10 2015-01-05 具有高准确度的单分子分析 Active CN106460034B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14150807.7 2014-01-10
EP14150807 2014-01-10
PCT/EP2015/050066 WO2015104245A1 (en) 2014-01-10 2015-01-05 Single molecule analysis with high accuracy

Publications (2)

Publication Number Publication Date
CN106460034A CN106460034A (zh) 2017-02-22
CN106460034B true CN106460034B (zh) 2021-05-07

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US (4) US11091805B2 (enExample)
EP (1) EP3092314B1 (enExample)
JP (1) JP6731854B2 (enExample)
CN (1) CN106460034B (enExample)
WO (1) WO2015104245A1 (enExample)

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US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
CN106460034B (zh) * 2014-01-10 2021-05-07 格诺特斯控股股份公司 具有高准确度的单分子分析
EP3112841A1 (en) 2015-06-30 2017-01-04 Gnothis Holding SA Single molecule analysis in an electrical field
EP3330763A1 (en) * 2016-12-05 2018-06-06 Gnothis AB Apparatus for characterizing luminescent entities
CN114921537A (zh) * 2017-03-17 2022-08-19 雅普顿生物系统公司 测序和高分辨率成像
CN108469429B (zh) * 2018-02-06 2020-11-24 西安电子科技大学 一种双模态拉曼-光学投影断层成像系统
WO2020061237A1 (en) 2018-09-19 2020-03-26 Apton Biosystems, Inc. Densely-packed analyte layers and detection methods
EP3896146A4 (en) * 2018-12-12 2022-01-19 GeneMind Biosciences Company Limited Optical system, method for calibrating optical system, and sequencing system
EP4616193A1 (en) 2022-11-07 2025-09-17 Gnothis Holding AG Selective-area coated protein resistant surface
EP4379378A1 (en) 2022-11-29 2024-06-05 Gnothis Holding AG Selective-area coated protein resistant surface
EP4623095A1 (en) 2022-11-21 2025-10-01 Gnothis Holding AG Multiple molecular binding sites
EP4403643A1 (en) 2023-01-19 2024-07-24 Gnothis Holding AG Multiple molecular binding sites
EP4375644A1 (en) 2022-11-22 2024-05-29 Gnothis Holding AG Fingerprint analysis of single molecule events
EP4379355A1 (en) 2022-11-29 2024-06-05 Gnothis Holding AG High throughput analysis of single molecule events
EP4382614A1 (en) 2022-12-06 2024-06-12 Gnothis Holding AG High yield support

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DK0679251T3 (da) 1993-01-18 1999-01-25 Evotec Biosystems Aktiengesell Fremgangsmåde og apparat til vurdering af biopolymerers fitness
DE10126083A1 (de) 2001-05-29 2002-12-05 Gnothis Holding Sa Ecublens Verwendung von optischen Diffraktionselementen in Nachweisverfahren
DE10162536A1 (de) 2001-12-19 2003-07-17 Gnothis Holding Sa Ecublens Evaneszenz-basierendes Multiplex-Sequenzierungsverfahren
DE102004038359A1 (de) 2004-08-06 2006-03-16 Rudolf Rigler Paralleles Hochdurchsatz-Einzelmolekül-Sequenzierungsverfahren
US7692783B2 (en) * 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US7715001B2 (en) * 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
JP5337676B2 (ja) * 2009-06-25 2013-11-06 株式会社日立ハイテクノロジーズ 蛍光分析装置および蛍光検出装置
JP5372876B2 (ja) * 2010-09-10 2013-12-18 株式会社日立ハイテクノロジーズ 核酸分析デバイス，核酸分析装置、及び核酸分析方法
WO2013131888A1 (en) 2012-03-06 2013-09-12 Rudolf Rigler Cyclic single molecule sequencing process
CN106460034B (zh) * 2014-01-10 2021-05-07 格诺特斯控股股份公司 具有高准确度的单分子分析
EP3112841A1 (en) * 2015-06-30 2017-01-04 Gnothis Holding SA Single molecule analysis in an electrical field

Non-Patent Citations (2)

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High一throughput FCS using an LCOS spatial light modulator and an 8 x 1 SPAD array;RYAN A. COLYER ET AL;《BIOMEDICAL OPTICS EXPRESS》;20101115;第1卷(第5期);1408-1431 *
Toward single-molecule detection with very compact DNA sequencer based on single-photon avalanche diode array;IVAN RECH ET AL;《PROCEEDINGS OF SPIE》;20080207;第6862卷;68620D-1 *

Also Published As

Publication number Publication date
CN106460034A (zh) 2017-02-22
EP3092314A1 (en) 2016-11-16
WO2015104245A1 (en) 2015-07-16
US20160326581A1 (en) 2016-11-10
US20230295720A1 (en) 2023-09-21
US12305232B2 (en) 2025-05-20
EP3092314B1 (en) 2019-12-11
US11091805B2 (en) 2021-08-17
US20220002800A1 (en) 2022-01-06
JP2017504039A (ja) 2017-02-02
US11788140B2 (en) 2023-10-17
JP6731854B2 (ja) 2020-07-29
US20250257397A1 (en) 2025-08-14

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