JP2017501148A5 - - Google Patents
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- Publication number
- JP2017501148A5 JP2017501148A5 JP2016538783A JP2016538783A JP2017501148A5 JP 2017501148 A5 JP2017501148 A5 JP 2017501148A5 JP 2016538783 A JP2016538783 A JP 2016538783A JP 2016538783 A JP2016538783 A JP 2016538783A JP 2017501148 A5 JP2017501148 A5 JP 2017501148A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- mixture
- conjugation
- chemical moiety
- reduced
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 17
- 239000000203 mixture Substances 0.000 claims 16
- 235000018102 proteins Nutrition 0.000 claims 11
- 102000004169 proteins and genes Human genes 0.000 claims 11
- 108090000623 proteins and genes Proteins 0.000 claims 11
- 230000021615 conjugation Effects 0.000 claims 7
- 239000000126 substance Substances 0.000 claims 5
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 claims 4
- 239000003638 chemical reducing agent Substances 0.000 claims 4
- 238000006722 reduction reaction Methods 0.000 claims 4
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 3
- 238000002360 preparation method Methods 0.000 claims 3
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 2
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 claims 2
- 238000009295 crossflow filtration Methods 0.000 claims 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 2
- 102000009027 Albumins Human genes 0.000 claims 1
- 108010088751 Albumins Proteins 0.000 claims 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 claims 1
- 108010024636 Glutathione Proteins 0.000 claims 1
- 102000018997 Growth Hormone Human genes 0.000 claims 1
- 108010051696 Growth Hormone Proteins 0.000 claims 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- 229960002433 cysteine Drugs 0.000 claims 1
- 238000011026 diafiltration Methods 0.000 claims 1
- 239000012538 diafiltration buffer Substances 0.000 claims 1
- 229960003180 glutathione Drugs 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 229960003151 mercaptamine Drugs 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 229910000073 phosphorus hydride Inorganic materials 0.000 claims 1
- 150000003568 thioethers Chemical class 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13197150 | 2013-12-13 | ||
| EP13197150.9 | 2013-12-13 | ||
| PCT/EP2014/077777 WO2015086853A1 (en) | 2013-12-13 | 2014-12-15 | Method for thioether conjugation of proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017501148A JP2017501148A (ja) | 2017-01-12 |
| JP2017501148A5 true JP2017501148A5 (https=) | 2018-01-25 |
| JP6603662B2 JP6603662B2 (ja) | 2019-11-06 |
Family
ID=49765887
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016538783A Active JP6603662B2 (ja) | 2013-12-13 | 2014-12-15 | タンパク質のチオエーテルコンジュゲーション方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11219690B2 (https=) |
| EP (1) | EP3079722B1 (https=) |
| JP (1) | JP6603662B2 (https=) |
| CN (1) | CN106068127B (https=) |
| ES (1) | ES2869309T3 (https=) |
| WO (1) | WO2015086853A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA127495C2 (uk) | 2015-12-23 | 2023-09-13 | Амджен Інк. | Виділений антигензв'язуючий білок, який специфічно зв'язується з поліпептидом рецептора шлункового інгібіторного пептиду (gipr) людини, та фармацевтична композиція, яка його містить |
| IL313587A (en) | 2017-01-17 | 2024-08-01 | Amgen Inc | Method of treating or ameliorating metabolic disorders using glp-1 receptor agonists conjugated to antagonists for gastric inhibitory peptide receptor (gipr) |
| EA201992502A1 (ru) | 2017-06-20 | 2020-04-22 | Эмджен Инк. | Способ лечения или уменьшения интенсивности метаболических нарушений с применением белков, связывающих рецептор гастроингибиторного пептида (gipr), в комбинации с агонистами glp-1 |
| EP3642238A1 (en) | 2017-06-21 | 2020-04-29 | Amgen Inc. | Method of treating or ameliorating metabolic disorders using antagonistic binding proteins for gastric inhibitory peptide receptor (gipr)/glp-1 receptor agonist fusion proteins |
| LT3661562T (lt) * | 2017-08-04 | 2024-10-25 | Amgen Inc. | Cys-mab konjugavimo būdas |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2297889T3 (es) | 1997-07-14 | 2008-05-01 | Bolder Biotechnology, Inc. | Derivados de hormona de crecimiento y proteinas relacionadas. |
| US6753165B1 (en) | 1999-01-14 | 2004-06-22 | Bolder Biotechnology, Inc. | Methods for making proteins containing free cysteine residues |
| CA2359345A1 (en) | 1999-01-14 | 2000-07-20 | Bolder Biotechnology, Inc. | Methods for making proteins containing free cysteine residues |
| IL152804A0 (en) * | 2000-05-16 | 2003-06-24 | Bolder Biotechnology Inc | Methods for refolding proteins containing free cysteine residues |
| WO2004101597A2 (en) | 2003-05-13 | 2004-11-25 | Frutarom Ltd. | Methods for the reduction of disulfide bonds |
| ES2642214T3 (es) | 2004-01-21 | 2017-11-15 | Novo Nordisk Health Care Ag | Conjugación de péptidos mediante transglutaminasa |
| ES2553160T3 (es) * | 2005-06-17 | 2015-12-04 | Novo Nordisk Health Care Ag | Reducción y derivatización selectivas de proteínas Factor VII transformadas por ingeniería que comprenden al menos una cisteína no nativa |
| GB0513852D0 (en) * | 2005-07-06 | 2005-08-10 | Celltech R&D Ltd | Biological products |
| WO2007109475A2 (en) * | 2006-03-16 | 2007-09-27 | Bracco Imaging S.P.A. | Chelation of metals to thiol groups using in situ reduction of disulfide-containing compounds by phosphines |
| JP5703020B2 (ja) | 2007-08-24 | 2015-04-15 | ノボ ノルディスク ヘルス ケア アーゲー | タンパク質を選択的に修飾するための組成物と方法 |
| RS59459B1 (sr) | 2010-01-22 | 2019-11-29 | Novo Nordisk Healthcare Ag | Hormoni rasta sa produženom in-vivo efikasnošću |
| MX338357B (es) | 2010-01-22 | 2016-04-13 | Novo Nordisk Healthcare Ag | Compuestos estables de la hormona de crecimiento. |
| ES2582590T3 (es) | 2010-02-16 | 2016-09-13 | Novo Nordisk A/S | Método de purificación |
| EP2595661A1 (en) | 2010-07-22 | 2013-05-29 | Novo Nordisk Health Care AG | Growth hormone conjugates |
| JP2014520094A (ja) | 2011-05-27 | 2014-08-21 | バクスター・インターナショナル・インコーポレイテッド | 増加した半減期を有する治療用タンパク質およびそれを調製する方法 |
| HUE035685T2 (en) * | 2011-11-02 | 2018-05-28 | Hoffmann La Roche | Overload and elute chromatography |
| CN103087183A (zh) * | 2013-01-17 | 2013-05-08 | 南京邮电大学 | 一种光激发蛋白质中二硫键还原得到自由巯基的方法 |
-
2014
- 2014-12-15 EP EP14818938.4A patent/EP3079722B1/en active Active
- 2014-12-15 JP JP2016538783A patent/JP6603662B2/ja active Active
- 2014-12-15 CN CN201480068335.5A patent/CN106068127B/zh active Active
- 2014-12-15 US US15/103,482 patent/US11219690B2/en active Active
- 2014-12-15 WO PCT/EP2014/077777 patent/WO2015086853A1/en not_active Ceased
- 2014-12-15 ES ES14818938T patent/ES2869309T3/es active Active
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