JP2016533723A5 - - Google Patents

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JP2016533723A5
JP2016533723A5 JP2016524559A JP2016524559A JP2016533723A5 JP 2016533723 A5 JP2016533723 A5 JP 2016533723A5 JP 2016524559 A JP2016524559 A JP 2016524559A JP 2016524559 A JP2016524559 A JP 2016524559A JP 2016533723 A5 JP2016533723 A5 JP 2016533723A5
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well
biochip
electrodes
sidewall
film
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JP2016533723A (ja
JP6514692B2 (ja
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Priority claimed from PCT/US2014/061854 external-priority patent/WO2015061511A1/en
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JP2016524559A 2013-10-23 2014-10-23 バイオセンサウェル形成のためのプロセス Active JP6514692B2 (ja)

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US201361894661P 2013-10-23 2013-10-23
US61/894,661 2013-10-23
PCT/US2014/061854 WO2015061511A1 (en) 2013-10-23 2014-10-23 Process for biosensor well formation

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JP2016533723A JP2016533723A (ja) 2016-11-04
JP2016533723A5 true JP2016533723A5 (enExample) 2017-06-29
JP6514692B2 JP6514692B2 (ja) 2019-05-15

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JP2016524578A Active JP6684209B2 (ja) 2013-10-23 2014-10-23 バイオチップ上に脂質二重層を形成するための方法
JP2016524559A Active JP6514692B2 (ja) 2013-10-23 2014-10-23 バイオセンサウェル形成のためのプロセス

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US (7) US9567630B2 (enExample)
EP (3) EP3060647B1 (enExample)
JP (2) JP6684209B2 (enExample)
CN (4) CN105637081B (enExample)
CA (2) CA2926140C (enExample)
ES (1) ES2779073T3 (enExample)
WO (2) WO2015061511A1 (enExample)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4107269B2 (ja) * 2004-02-23 2008-06-25 ソニー株式会社 固体撮像装置
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
US10908143B2 (en) * 2013-11-27 2021-02-02 Hitachi, Ltd. Current measuring device, current measuring method, and current measuring kit
WO2016004029A1 (en) * 2014-06-30 2016-01-07 The Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods of preparing stabilized lipid assemblies
CN104792845A (zh) * 2014-08-07 2015-07-22 中国科学院微电子研究所 传感装置
WO2016069806A2 (en) 2014-10-31 2016-05-06 Genia Technologies, Inc. Alpha-hemolysin variants with altered characteristics
USD815752S1 (en) * 2014-11-28 2018-04-17 Randox Laboratories Ltd. Biochip well
US9658190B2 (en) * 2014-12-18 2017-05-23 Genia Technologies, Inc. Printed electrode
US10036739B2 (en) 2015-01-27 2018-07-31 Genia Technologies, Inc. Adjustable bilayer capacitance structure for biomedical devices
WO2016124543A1 (en) 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants
DE102015106032B4 (de) 2015-04-20 2018-10-31 Gabriel Krajewski Vorrichtung und Verfahren zur manuellen Handhabung einer Palette
US10526588B2 (en) 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
US10174371B2 (en) 2015-08-05 2019-01-08 Genia Technologies, Inc. Use of titanium nitride as an electrode in non-faradaic electrochemical cell
US10809243B2 (en) * 2015-08-31 2020-10-20 Roche Sequencing Solutions, Inc. Small aperture large electrode cell
US10920312B2 (en) * 2015-08-31 2021-02-16 Roche Sequencing Solutions, Inc. Electrochemical cell with increased current density
EP3353194B1 (en) 2015-09-22 2023-08-30 F. Hoffmann-La Roche AG Ompg variants
EP3766987B1 (en) 2015-09-24 2023-08-02 F. Hoffmann-La Roche AG Alpha-hemolysin variants
US20180299400A1 (en) * 2015-10-21 2018-10-18 Genia Technologies, Inc. Use of fluoropolymers as a hydrophobic layer to support lipid bilayer formation for nanopore based dna sequencing
US10718737B2 (en) 2016-01-21 2020-07-21 Roche Sequencing Solutions, Inc. Molded flow channel
WO2017125565A1 (en) * 2016-01-21 2017-07-27 Genia Technologies, Inc. Nanopore sequencing complexes
US10371664B2 (en) * 2016-01-21 2019-08-06 Roche Molecular Systems, Inc. Use of titanium nitride as a counter electrode
JP6690005B2 (ja) 2016-02-29 2020-04-28 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアシーケンシングのためのポリメラーゼ−鋳型複合体
ES2880331T3 (es) 2016-02-29 2021-11-24 Genia Tech Inc Variantes de polimerasa
US10590480B2 (en) 2016-02-29 2020-03-17 Roche Sequencing Solutions, Inc. Polymerase variants
JP6959931B2 (ja) 2016-02-29 2021-11-05 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ
US10465240B2 (en) * 2016-03-30 2019-11-05 Roche Sequencing Solutions, Inc. Electrical enhancement of bilayer formation
US10155979B2 (en) 2016-03-30 2018-12-18 Roche Molecular Systems, Inc. Non-destructive bilayer monitoring using measurement of bilayer response to electrical stimulus
US10233486B2 (en) 2016-03-30 2019-03-19 Roche Sequencing Solutions, Inc. Method for inline bilayer capacitance monitoring
EP3445775A1 (en) * 2016-04-21 2019-02-27 H. Hoffnabb-La Roche Ag Alpha-hemolysin variants and uses thereof
US10509006B2 (en) * 2016-06-20 2019-12-17 Axbio Inc. Devices and methods for measuring the properties of macromolecules
US10577653B2 (en) 2016-06-27 2020-03-03 Roche Sequencing Solutions, Inc. Counteracting osmotic imbalance in a sequencing cell
EP3475696B1 (en) * 2016-06-27 2020-04-08 H. Hoffnabb-La Roche Ag Osmotic imbalance methods for bilayer formation
CN109415419B (zh) 2016-06-30 2022-07-29 豪夫迈·罗氏有限公司 长寿α-溶血素纳米孔
JP7157048B2 (ja) 2016-09-22 2022-10-19 エフ．ホフマン－ラ ロシュ アーゲー Ｐｏｌ６ポリメラーゼバリアント
JP2018048950A (ja) * 2016-09-23 2018-03-29 株式会社東芝 分析チップ
US12319960B2 (en) * 2016-10-12 2025-06-03 Roche Sequencing Solutions, Inc. Nanopore voltage methods
CN110366680B (zh) 2017-02-14 2021-10-08 阿克斯比尔公司 用于大分子的连续诊断的设备和方法
US10739299B2 (en) * 2017-03-14 2020-08-11 Roche Sequencing Solutions, Inc. Nanopore well structures and methods
WO2018195222A1 (en) * 2017-04-19 2018-10-25 Electronic Biosciences, Inc. Nanopore/nanowell electrode enabled exonuclease sequencing
EP3673085A4 (en) 2017-08-23 2021-04-28 Roche Diagnostics GmbH ENZYME SCREENING PROCEDURE
DE102017130198A1 (de) 2017-12-15 2019-06-19 IMMS Institut für Mikroelektronik- und Mechatronik-Systeme gemeinnützige GmbH (IMMS GmbH) Analyseanordnung zur Durchführung biologischer und/oder chemischer Analysen von Substanzen sowie Verfahren zu seiner Herstellung
US20190204293A1 (en) * 2017-12-28 2019-07-04 Life Technologies Corporation Sensor for chemical analysis and methods for manufacturing the same
CN108226249B (zh) * 2018-01-09 2021-02-26 深圳市梅丽纳米孔科技有限公司 一次性纳米孔生物传感器及其制作方法
WO2019160925A1 (en) 2018-02-16 2019-08-22 Illumina, Inc. Device for sequencing
CN112204398B (zh) * 2018-02-16 2025-01-28 迪亚加斯特公司 包括珠的体外诊断装置及其用途
CN112041331B (zh) 2018-02-28 2024-05-28 豪夫迈·罗氏有限公司 α-溶血素变体及其用途
JP6825173B2 (ja) * 2018-03-15 2021-02-03 アイポア株式会社 分析素子
CN108709921B (zh) * 2018-04-02 2021-08-03 华东理工大学 一种利用多通道纳米孔道-膜检测系统的检测方法
WO2019213571A1 (en) 2018-05-03 2019-11-07 The Trustees Of Wheaton College Improved membranes for nanopore sensing applications
CN112534063A (zh) 2018-05-22 2021-03-19 安序源有限公司 用于核酸测序的方法、系统和组合物
WO2019228995A1 (en) 2018-05-28 2019-12-05 F. Hoffmann-La Roche Ag Enzymatic enrichment of dna-pore-polymerase complexes
JP2019219244A (ja) * 2018-06-19 2019-12-26 ソニーセミコンダクタソリューションズ株式会社 電位測定装置及び電位測定装置の製造方法
CN112714869B (zh) 2018-08-28 2024-06-18 豪夫迈·罗氏有限公司 包括含钌电极的纳米孔测序装置
WO2020055501A1 (en) * 2018-09-14 2020-03-19 Applied Materials, Inc. Method of forming a nanopore and resulting structure
CN113260449B (zh) * 2018-12-11 2023-09-29 豪夫迈·罗氏有限公司 用于膜中自限性蛋白质孔插入的系统和方法
EP3898643B1 (en) 2018-12-19 2024-04-17 Roche Diagnostics GmbH 3' reversibly protected nucleotides for sequencing by synthesis using nanopores
EP3711848B1 (en) * 2019-03-20 2023-12-20 Matthias Wilm Method for preparation of a bioactive lipid membrane and a functionalized lipid bi-layer membrane prepared with said method
JP7676380B2 (ja) * 2019-10-30 2025-05-14 エフ． ホフマン－ラ ロシュ アーゲー 生物学的ナノポアのアセンブリのための方法及び組成物
EP4052035A1 (en) 2019-10-31 2022-09-07 The Trustees of Wheaton College Design and characterization of multilayered structures for support of lipid bilayers
CN111123428B (zh) * 2019-12-24 2022-03-08 中国科学院苏州生物医学工程技术研究所 一种零模波导孔孔壁的修饰方法及零模波导孔结构
CN111123429B (zh) * 2019-12-24 2022-03-08 中国科学院苏州生物医学工程技术研究所 涂覆有锚定物质的零模波导孔的制备方法及波导孔结构
US11305288B2 (en) 2020-01-28 2022-04-19 Plexium, Inc. Assay devices for combinatorial libraries
WO2021184374A1 (en) 2020-03-20 2021-09-23 Genesense Technology Inc High throughput analytical system for molecule detection and sensing
WO2021219795A1 (en) * 2020-05-01 2021-11-04 F. Hoffmann-La Roche Ag Systems and methods for using trapped charge for bilayer formation and pore insertion in a nanopore array
KR102579071B1 (ko) * 2021-02-18 2023-09-15 고려대학교 산학협력단 포어 구조를 구비하는 핵산 검출용 미세 유동장치
CN113061531B (zh) * 2021-06-03 2021-08-20 成都齐碳科技有限公司 芯片结构、芯片组件、成膜方法、纳米孔测序装置及应用
CN113337860B (zh) * 2021-08-02 2021-11-09 华芯半导体研究院(北京)有限公司 在芯片晶圆表面进行电镀的方法及其应用
CN118318049A (zh) 2021-09-28 2024-07-09 安序源有限公司 用于处理核酸样品的方法以及核酸样品的组合物
US12455277B2 (en) 2021-09-30 2025-10-28 Illumina, Inc. Isolation of cells in a nanopore sensor array
AU2021481308A1 (en) * 2021-12-27 2024-08-08 Bgi Shenzhen Membrane forming device and membrane forming method
WO2023123292A1 (zh) * 2021-12-30 2023-07-06 深圳华大生命科学研究院 纳米孔生物传感器的制备方法及测序方法
AU2023264238A1 (en) 2022-05-02 2024-10-10 F. Hoffmann-La Roche Ag Compositions and methods that reduce prussian blue formation during nanopore sequencing
CN121013911A (zh) 2023-03-30 2025-11-25 豪夫迈·罗氏有限公司 靶分子-脂质双层相互作用的调节
WO2024227837A1 (en) 2023-05-04 2024-11-07 F. Hoffmann-La Roche Ag Hybrid triblock copolymer membrane compositions and methods for nanopore sequencing
WO2024227838A1 (en) 2023-05-04 2024-11-07 F. Hoffmann-La Roche Ag Modified triblock copolymer compounds and methods of use thereof
WO2024259020A2 (en) * 2023-06-12 2024-12-19 Northeastern University Nanopore and membrane protein multichannel measurement apparatus and fabrication method
DE102023209046A1 (de) 2023-09-18 2025-03-20 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung eingetragener Verein Vorrichtung mit nanopipetten-array zum durchführen von elektrophysiologischen messungen und verfahren zum herstellen dieser vorrichtung
WO2025078657A1 (en) 2023-10-13 2025-04-17 F. Hoffmann-La Roche Ag Amplification-free target enrichment workflow for direct detection of nucleic acid modifications
CN118291231A (zh) * 2024-03-29 2024-07-05 北京齐碳科技有限公司 孔阵列层结构和孔阵列层结构的制备方法
WO2025212586A1 (en) 2024-04-02 2025-10-09 Roche Sequencing Solutions, Inc. High throughput inramolecular consensus reads
WO2025210056A1 (en) 2024-04-03 2025-10-09 Roche Sequencing Solutions, Inc. In vitro amplification of dna methylation patterns

Family Cites Families (214)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2656508A (en) 1949-08-27 1953-10-20 Wallace H Coulter Means for counting particles suspended in a fluid
US4121192A (en) 1974-01-31 1978-10-17 Gte Sylvania Incorporated System and method for determining position and velocity of an intruder from an array of sensors
US4859945A (en) 1988-05-03 1989-08-22 Elscint Ltd. Optimized signal to noise ratio
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
JPH04236295A (ja) 1991-01-18 1992-08-25 Sharp Corp 強誘電性液晶組成物及び液晶素子
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
WO1993021528A1 (en) 1992-04-22 1993-10-28 Ecole Polytechnique Federale De Lausanne (Epfl) Lipid membrane sensors
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5457342A (en) 1994-03-30 1995-10-10 Herbst, Ii; Gerhardt G. Integrated circuit cooling apparatus
FR2722294B1 (fr) 1994-07-07 1996-10-04 Lyon Ecole Centrale Procede d'analyse qualitative et/ou quantitative de substances biologiques presentes dans un milieu liquide conducteur et capteurs biochimiques d'affinite utilises pour la mise en oeuvre de ce procede
JP2986381B2 (ja) 1994-08-16 1999-12-06 インターナショナル・ビジネス・マシーンズ・コーポレイション 電子チップ温度制御装置及び方法
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US6014213A (en) 1994-12-12 2000-01-11 Visible Genetics Inc. High dynamic range apparatus for separation and detection of polynucleotide fragments
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5780231A (en) 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US6261797B1 (en) 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
JP4124377B2 (ja) 1996-06-06 2008-07-23 ソレクサ・インコーポレイテッド コードアダプターの連結による配列決定
US5981733A (en) 1996-09-16 1999-11-09 Incyte Pharmaceuticals, Inc. Apparatus for the chemical synthesis of molecular arrays
US6699719B2 (en) 1996-11-29 2004-03-02 Proteomic Systems, Inc. Biosensor arrays and methods
JPH10187253A (ja) 1996-12-27 1998-07-14 Ando Electric Co Ltd 光半導体素子の温度制御装置
US5804386A (en) 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US5876936A (en) 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
AU743025B2 (en) 1997-03-12 2002-01-17 Applera Corporation DNA polymerases having improved labeled nucleotide incorporation properties
US6002263A (en) 1997-06-06 1999-12-14 Cascade Microtech, Inc. Probe station having inner and outer shielding
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
SE9703958D0 (sv) 1997-10-29 1997-10-29 Pacesetter Ab Method and device for determination of concentration
EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
US6232103B1 (en) 1998-03-23 2001-05-15 Invitrogen Corporation Methods useful for nucleic acid sequencing using modified nucleotides comprising phenylboronic acid
US6485703B1 (en) 1998-07-31 2002-11-26 The Texas A&M University System Compositions and methods for analyte detection
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6217731B1 (en) 1998-10-21 2001-04-17 Spectrumedix Corporation Method and apparatus for monitoring and displaying the status of a parallel capillary electrophoresis device
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
JP2002532717A (ja) 1998-12-11 2002-10-02 サイミックス テクノロジーズ、インク 迅速な物質特性評価のためのセンサ配列に基づくシステム及びその方法
EP1141409B2 (en) 1998-12-14 2009-05-27 Pacific Biosciences of California, Inc. A kit and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6082115A (en) 1998-12-18 2000-07-04 National Semiconductor Corporation Temperature regulator circuit and precision voltage reference for integrated circuit
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US20030054360A1 (en) 1999-01-19 2003-03-20 Larry Gold Method and apparatus for the automated generation of nucleic acid ligands
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6383749B2 (en) 1999-12-02 2002-05-07 Clontech Laboratories, Inc. Methods of labeling nucleic acids for use in array based hybridization assays
WO2001059453A2 (en) 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
US6616895B2 (en) 2000-03-23 2003-09-09 Advanced Research Corporation Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
ATE384731T1 (de) 2000-08-03 2008-02-15 Hoffmann La Roche Nukleinsäurebindende verbindungen mit pyrazolo 3, 4-d pyrimidinanalogen von purin-2,6-diamin und ihre verwendung
AU2001286963A1 (en) 2000-08-30 2002-03-13 University Of Rochester Method of performing reverse transcription reaction using reverse transcriptase encoded by non-ltr retrotransposable element
CA2421582A1 (en) 2000-09-11 2002-03-21 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and uses thereof
US20060057565A1 (en) 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
EP1790736A3 (en) 2000-10-06 2007-08-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7348183B2 (en) * 2000-10-16 2008-03-25 Board Of Trustees Of The University Of Arkansas Self-contained microelectrochemical bioassay platforms and methods
US20030027140A1 (en) 2001-03-30 2003-02-06 Jingyue Ju High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry
DE10120083A1 (de) * 2001-04-17 2003-01-30 Nmi Univ Tuebingen Meßelektrodenpaar, Biosensor mit einem solchen Meßelektrodenpaar und Verfahren zur Herstellung
US6800933B1 (en) 2001-04-23 2004-10-05 Advanced Micro Devices, Inc. Integrated circuit cooling device
US6686997B1 (en) 2001-08-27 2004-02-03 Raytheon Company Apparatus and a method for pulse detection and characterization
US7052839B2 (en) 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
DK1421213T3 (da) 2001-08-29 2010-06-07 Ge Healthcare Bio Sciences Mærkede nukleosidpolyphosphater
US7727722B2 (en) 2001-08-29 2010-06-01 General Electric Company Ligation amplification
US7033762B2 (en) 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
WO2003025132A2 (en) 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
WO2003034026A2 (en) 2001-10-15 2003-04-24 Biocept, Inc. Microwell biochip
US20050014201A1 (en) * 2001-10-25 2005-01-20 Mordechai Deuthsch Interactive transparent individual cells biochip processor
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
EP1572902B1 (en) 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US6952651B2 (en) 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
US7948041B2 (en) 2005-05-19 2011-05-24 Nanomix, Inc. Sensor having a thin-film inhibition layer
US7074597B2 (en) 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
DE10238843B8 (de) 2002-08-20 2008-01-03 Infineon Technologies Ag Halbleiterbauelement
CA2497239A1 (en) 2002-08-27 2004-04-29 James Chris Sackellares Optimization of multi-dimensional time series processing for seizure warning and prediction
US7355216B2 (en) 2002-12-09 2008-04-08 The Regents Of The University Of California Fluidic nanotubes and devices
AU2003297859A1 (en) 2002-12-13 2004-07-09 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
US7393640B2 (en) 2003-02-05 2008-07-01 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides with new linkers
AU2004211920B2 (en) 2003-02-05 2009-05-14 Global Life Sciences Solutions Usa Llc Solid phase sequencing
JP2006526777A (ja) 2003-02-28 2006-11-24 ブラウン ユニバーシティー ナノ細孔、その使用方法、その作成方法、及びそれを使用して生体分子を特徴付ける方法
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0308851D0 (en) 2003-04-16 2003-05-21 Lingvitae As Method
WO2005003722A2 (en) 2003-06-13 2005-01-13 North Carolina State University Nanotube structures having a surfactant bilayer inner wall coating
US8233959B2 (en) 2003-08-22 2012-07-31 Dexcom, Inc. Systems and methods for processing analyte sensor data
US20050079598A1 (en) 2003-10-08 2005-04-14 Davis Randall W. Apparatus and method for identification of biomolecules, in particular nucleic acid sequences, proteins, and antigens and antibodies
US6880345B1 (en) 2003-11-04 2005-04-19 Intel Corporation Cooling system for an electronic component
JP4497903B2 (ja) * 2003-12-02 2010-07-07 財団法人大阪産業振興機構 タンパク質チップおよびそれを用いたバイオセンサー
US7019346B2 (en) 2003-12-23 2006-03-28 Intel Corporation Capacitor having an anodic metal oxide substrate
US20050186576A1 (en) 2004-02-19 2005-08-25 Intel Corporation Polymer sequencing using selectively labeled monomers and data integration
JP4107269B2 (ja) 2004-02-23 2008-06-25 ソニー株式会社 固体撮像装置
WO2005084367A2 (en) 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
US7279337B2 (en) 2004-03-10 2007-10-09 Agilent Technologies, Inc. Method and apparatus for sequencing polymers through tunneling conductance variation detection
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US20050221351A1 (en) 2004-04-06 2005-10-06 Affymetrix, Inc. Methods and devices for microarray image analysis
US20050239134A1 (en) 2004-04-21 2005-10-27 Board Of Regents, The University Of Texas System Combinatorial selection of phosphorothioate single-stranded DNA aptamers for TGF-beta-1 protein
JP2006004079A (ja) 2004-06-16 2006-01-05 Sony Corp 記憶装置
US6880346B1 (en) 2004-07-08 2005-04-19 Giga-Byte Technology Co., Ltd. Two stage radiation thermoelectric cooling apparatus
AT501510B1 (de) 2004-07-19 2009-05-15 Univ Wien Tech Dezentrale fehlertolerante taktgenerierung in vlsi chips
US7622934B2 (en) 2004-07-23 2009-11-24 Electronic Bio Sciences, Llc Method and apparatus for sensing a time varying current passing through an ion channel
US7972858B2 (en) 2004-08-13 2011-07-05 President And Fellows Of Harvard College Ultra high-throughput opti-nanopore DNA readout platform
GB0422733D0 (en) 2004-10-13 2004-11-17 Lingvitae As Method
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
EP1842061A4 (en) 2004-12-21 2009-05-13 Texas A & M Univ Sys HIGH-TEMPERATURE ION CHANNELS AND ION PORES
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7767493B2 (en) 2005-06-14 2010-08-03 John Trezza Post & penetration interconnection
US9169510B2 (en) 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
JP2008546424A (ja) 2005-06-28 2008-12-25 アジェンコート パーソナル ジェノミクス コーポレイション 修飾ポリヌクレオチドを作製する方法および配列決定する方法
JP4524652B2 (ja) 2005-07-06 2010-08-18 ソニー株式会社 Ａｄ変換装置並びに半導体装置
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US20070190542A1 (en) 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
US7397232B2 (en) 2005-10-21 2008-07-08 The University Of Akron Coulter counter having a plurality of channels
GB2446084B (en) 2005-10-31 2011-03-02 Univ Columbia Synthesis of four color 3-o-allyl modified photocleavable fluorescent nucleotides and related methods
US8796432B2 (en) 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
US20090088332A1 (en) 2005-11-21 2009-04-02 Jingyue Ju Multiplex Digital Immuno-Sensing Using a Library of Photocleavable Mass Tags
JP2009519041A (ja) 2005-12-12 2009-05-14 アメリカ合衆国 核酸配列決定のためのプローブおよび使用方法
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
ATE518010T1 (de) 2005-12-22 2011-08-15 Pacific Biosciences California Aktive oberflächengekoppelte polymerasen
JP4735833B2 (ja) * 2006-01-13 2011-07-27 セイコーエプソン株式会社 バイオチップ及びバイオセンサ
US8002712B2 (en) 2006-01-26 2011-08-23 Meka Vikas V Breath and breath condensate analysis system and associated methods
US7368668B2 (en) 2006-02-03 2008-05-06 Freescale Semiconductor Inc. Ground shields for semiconductors
JP2009156572A (ja) * 2006-04-06 2009-07-16 National Institutes Of Natural Sciences イオンチャンネルタンパク質バイオセンサー
US20070298511A1 (en) 2006-04-27 2007-12-27 The Texas A&M University System Nanopore sensor system
US7777505B2 (en) * 2006-05-05 2010-08-17 University Of Utah Research Foundation Nanopore platforms for ion channel recordings and single molecule detection and analysis
US7446017B2 (en) 2006-05-31 2008-11-04 Freescale Semiconductor, Inc. Methods and apparatus for RF shielding in vertically-integrated semiconductor devices
CN101495656B (zh) 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
BRPI0603825A (pt) 2006-09-19 2008-05-06 Syngenta Protecao De Cultivos composição reguladora de crescimento de brotos em plantações de fumo, método de controle de crescimento de brotos em plantações de fumo e uso de uma quantidade eficaz de flumetralin, pelo menos um solvente e pelo menos um tensoativo
US9632073B2 (en) * 2012-04-02 2017-04-25 Lux Bio Group, Inc. Apparatus and method for molecular separation, purification, and sensing
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
DK2122344T3 (da) 2007-02-20 2019-07-15 Oxford Nanopore Tech Ltd Lipiddobbeltlags-sensorsystem
KR101521990B1 (ko) 2007-04-04 2015-05-20 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 나노포어 사용을 위한 조성물, 장치, 시스템 및 방법
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
JP5745842B2 (ja) 2007-06-19 2015-07-08 ストラトス ゲノミクス インコーポレイテッド 拡張によるハイスループット核酸配列決定
US20090073293A1 (en) 2007-06-27 2009-03-19 Yoel Yaffe CMOS image sensors with increased dynamic range and methods of operating the same
JP4929090B2 (ja) 2007-07-26 2012-05-09 パナソニック株式会社 固体撮像装置およびその駆動方法
EP2201136B1 (en) 2007-10-01 2017-12-06 Nabsys 2.0 LLC Nanopore sequencing by hybridization of probes to form ternary complexes and variable range alignment
JP5309145B2 (ja) 2007-10-02 2013-10-09 プレジデント アンド フェロウズ オブ ハーバード カレッジ ナノポアによる分子の捕捉、再捕捉およびトラッピング
EP2207900B1 (en) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
EP3431615A3 (en) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna sequencing with non-fluorescent nucleotide reversible terminators cleavable label modified nucleotide terminators
CN101910410A (zh) 2007-10-23 2010-12-08 斯特拉托斯基因公司 通过间隔进行高通量核酸测序
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US7899996B1 (en) * 2007-12-31 2011-03-01 Emc Corporation Full track read for adaptive pre-fetching of data
US20110165652A1 (en) 2008-01-14 2011-07-07 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
US7989928B2 (en) 2008-02-05 2011-08-02 Advanced Semiconductor Engineering Inc. Semiconductor device packages with electromagnetic interference shielding
US8022511B2 (en) 2008-02-05 2011-09-20 Advanced Semiconductor Engineering, Inc. Semiconductor device packages with electromagnetic interference shielding
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
AU2009215193A1 (en) 2008-02-12 2009-08-20 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US20090215050A1 (en) 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
EP2274446B1 (en) 2008-03-31 2015-09-09 Pacific Biosciences of California, Inc. Two slow-step polymerase enzyme systems and methods
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US9127259B2 (en) 2008-03-31 2015-09-08 Pacific Biosciences Of California, Inc. Enzymes resistant to photodamage
WO2009145820A2 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
EP2107040B1 (en) 2008-03-31 2011-10-26 Sony Deutschland Gmbh A method of fabricating a membrane having a tapered pore
EP2279194A1 (en) 2008-04-29 2011-02-02 Life Technologies Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use
US8940142B2 (en) 2008-05-05 2015-01-27 The Regents Of The University Of California Functionalized nanopipette biosensor
US20100122907A1 (en) 2008-05-06 2010-05-20 Government of the United States of America, Single molecule mass or size spectrometry in solution using a solitary nanopore
US7906371B2 (en) 2008-05-28 2011-03-15 Stats Chippac, Ltd. Semiconductor device and method of forming holes in substrate to interconnect top shield and ground shield
US20100025238A1 (en) 2008-07-31 2010-02-04 Medtronic Minimed, Inc. Analyte sensor apparatuses having improved electrode configurations and methods for making and using them
CA2735979A1 (en) 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Engineering polymerases and reaction conditions for modified incorporation properties
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US8063469B2 (en) 2008-09-30 2011-11-22 Infineon Technologies Ag On-chip radio frequency shield with interconnect metallization
WO2013154750A1 (en) * 2012-04-10 2013-10-17 The Trustees Of Columbia Unversity In The City Of New York Systems and methods for biological ion channel interfaces
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
CN102257162A (zh) 2008-10-29 2011-11-23 波士顿大学理事会 保持序列的dna转化
US8486630B2 (en) 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
US20100148126A1 (en) 2008-11-26 2010-06-17 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
WO2010123594A2 (en) 2009-01-15 2010-10-28 Children's Medical Center Corporation Device for filtration of fluids there through and accompanying method
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010111605A2 (en) 2009-03-27 2010-09-30 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US8986928B2 (en) * 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
US8926904B2 (en) * 2009-05-12 2015-01-06 Daniel Wai-Cheong So Method and apparatus for the analysis and identification of molecules
WO2011038241A1 (en) 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
CA2808576A1 (en) 2009-09-30 2011-04-07 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
EP2496936B1 (en) * 2009-10-16 2018-02-21 Microchips Biotech, Inc. Multi-channel potentiostat for biosensor arrays and method of operation
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
US20110287414A1 (en) 2010-02-08 2011-11-24 Genia Technologies, Inc. Systems and methods for identifying a portion of a molecule
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US20110192723A1 (en) 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US20120052188A1 (en) 2010-02-08 2012-03-01 Genia Technologies, Inc. Systems and methods for assembling a lipid bilayer on a substantially planar solid surface
CN103298984B (zh) * 2010-02-08 2015-11-25 吉尼亚科技公司 用于在纳米孔中操作分子的系统和方法
JP5159808B2 (ja) * 2010-02-09 2013-03-13 日本電信電話株式会社 脂質二分子膜基板
FI2539465T3 (fi) 2010-02-23 2024-03-27 Univ Washington Analyyttien sekvensointi nanohuokosten avulla
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
DE102010022929B4 (de) * 2010-06-07 2013-07-18 Albert-Ludwigs-Universität Freiburg Verfahren zum Herstellen einer Bilipidschicht sowie Mikrostruktur und Messanordnung
US9280596B2 (en) * 2010-07-01 2016-03-08 Salesforce.Com, Inc. Method and system for scoring articles in an on-demand services environment
CA2805247C (en) 2010-07-14 2021-08-10 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
EP2652153B1 (en) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
WO2012121756A1 (en) 2011-03-04 2012-09-13 Quantapore, Inc. Apparatus and methods for performing optical nanopore detection or sequencing
US20130087467A1 (en) * 2011-06-24 2013-04-11 Hongjun Yang High-performance analytical instrument and method
WO2013063126A2 (en) * 2011-10-25 2013-05-02 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Programmable arrays
JP5770615B2 (ja) * 2011-12-08 2015-08-26 日本電信電話株式会社 脂質二分子膜基板の製造方法
WO2013109970A1 (en) 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
US8541849B2 (en) 2012-02-14 2013-09-24 Genia Technologies, Inc. Noise shielding techniques for ultra low current measurements in biochemical applications
ES2732256T3 (es) 2012-02-16 2019-11-21 Genia Tech Inc Procedimientos para crear bicapas para su uso con sensores con nanoporos
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
US10029915B2 (en) 2012-04-04 2018-07-24 International Business Machines Corporation Functionally switchable self-assembled coating compound for controlling translocation of molecule through nanopores
JP6456816B2 (ja) 2012-04-09 2019-01-23 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク ナノ細孔の調製方法およびその使用
GB2510719A (en) 2012-06-15 2014-08-13 Genia Technologies Inc Chip set-up and high-accuracy nucleic acid sequencing
US20150119259A1 (en) 2012-06-20 2015-04-30 Jingyue Ju Nucleic acid sequencing by nanopore detection of tag molecules
US9513238B2 (en) 2012-11-29 2016-12-06 Helmut Fischer GmbH Institut für Elektronik und Messtechnik Method and device for performing an x-ray fluorescence analysis
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips

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