JP2016529920A5 - - Google Patents
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- JP2016529920A5 JP2016529920A5 JP2016543968A JP2016543968A JP2016529920A5 JP 2016529920 A5 JP2016529920 A5 JP 2016529920A5 JP 2016543968 A JP2016543968 A JP 2016543968A JP 2016543968 A JP2016543968 A JP 2016543968A JP 2016529920 A5 JP2016529920 A5 JP 2016529920A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- ihep
- cell population
- asc
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims description 91
- 238000000034 method Methods 0.000 claims description 65
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 19
- 102000004140 Oncostatin M Human genes 0.000 claims description 10
- 108090000630 Oncostatin M Proteins 0.000 claims description 10
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims description 9
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims description 9
- 229940126864 fibroblast growth factor Drugs 0.000 claims description 9
- 210000003494 hepatocyte Anatomy 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000003550 marker Substances 0.000 claims description 9
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 claims description 6
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 claims description 6
- 102000013814 Wnt Human genes 0.000 claims description 6
- 108050003627 Wnt Proteins 0.000 claims description 6
- 239000000556 agonist Substances 0.000 claims description 6
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims description 6
- 239000002243 precursor Substances 0.000 claims description 6
- 230000011664 signaling Effects 0.000 claims description 6
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 claims description 5
- 229960003957 dexamethasone Drugs 0.000 claims description 5
- 241000124008 Mammalia Species 0.000 claims description 4
- 210000004185 liver Anatomy 0.000 claims description 4
- 230000003908 liver function Effects 0.000 claims description 4
- 102100028072 Fibroblast growth factor 4 Human genes 0.000 claims description 3
- 101001060274 Homo sapiens Fibroblast growth factor 4 Proteins 0.000 claims description 3
- 108010023082 activin A Proteins 0.000 claims description 3
- 239000011230 binding agent Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 230000009870 specific binding Effects 0.000 claims description 3
- 210000000130 stem cell Anatomy 0.000 claims description 3
- 238000004114 suspension culture Methods 0.000 claims description 3
- 210000001789 adipocyte Anatomy 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims 7
- 230000006698 induction Effects 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- 206010019663 Hepatic failure Diseases 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 208000007903 liver failure Diseases 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 229960005489 paracetamol Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361880056P | 2013-09-19 | 2013-09-19 | |
| US61/880,056 | 2013-09-19 | ||
| PCT/US2014/056049 WO2015042125A2 (en) | 2013-09-19 | 2014-09-17 | Methods and compositions for producing hepatocyte-like cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020055548A Division JP7057797B2 (ja) | 2013-09-19 | 2020-03-26 | 肝細胞様細胞を産生するための方法および組成物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016529920A JP2016529920A (ja) | 2016-09-29 |
| JP2016529920A5 true JP2016529920A5 (cg-RX-API-DMAC7.html) | 2017-10-12 |
| JP6683615B2 JP6683615B2 (ja) | 2020-04-22 |
Family
ID=52689587
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016543968A Active JP6683615B2 (ja) | 2013-09-19 | 2014-09-17 | 肝細胞様細胞を産生するための方法および組成物 |
| JP2020055548A Active JP7057797B2 (ja) | 2013-09-19 | 2020-03-26 | 肝細胞様細胞を産生するための方法および組成物 |
| JP2022064376A Pending JP2022088668A (ja) | 2013-09-19 | 2022-04-08 | 肝細胞様細胞を産生するための方法および組成物 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020055548A Active JP7057797B2 (ja) | 2013-09-19 | 2020-03-26 | 肝細胞様細胞を産生するための方法および組成物 |
| JP2022064376A Pending JP2022088668A (ja) | 2013-09-19 | 2022-04-08 | 肝細胞様細胞を産生するための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10004767B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP3685884A1 (cg-RX-API-DMAC7.html) |
| JP (3) | JP6683615B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2959117C (cg-RX-API-DMAC7.html) |
| DK (1) | DK3046628T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2767062T3 (cg-RX-API-DMAC7.html) |
| SG (3) | SG10201802231RA (cg-RX-API-DMAC7.html) |
| WO (1) | WO2015042125A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| US20200297474A1 (en) * | 2017-10-05 | 2020-09-24 | The Johns Hopkins University | Implantable bioreactor and methods for making and using same |
| CN109749981B (zh) * | 2017-11-06 | 2023-10-10 | 博品(上海)生物医药科技有限公司 | 人源脂肪干细胞来源的肝细胞样细胞及其制备方法和应用 |
| JP7548583B2 (ja) * | 2018-09-20 | 2024-09-10 | ノヴァディップ バイオサイエンシズ | 脂肪由来幹細胞およびゼラチンを含む生体材料ならびにその製造方法 |
| EP4118188A1 (en) | 2020-03-11 | 2023-01-18 | Bit Bio Limited | Method of generating hepatic cells |
| TW202321440A (zh) | 2021-10-07 | 2023-06-01 | 美商肝特斯公司 | 追蹤接受者中之供體細胞之方法 |
| US20230174949A1 (en) * | 2021-12-03 | 2023-06-08 | The Regents Of The University Of California | Engineered vascularized organoids |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4491632A (en) | 1979-10-22 | 1985-01-01 | The Massachusetts General Hospital | Process for producing antibodies to hepatitis virus and cell lines therefor |
| US4444887A (en) | 1979-12-10 | 1984-04-24 | Sloan-Kettering Institute | Process for making human antibody producing B-lymphocytes |
| DE3167442D1 (en) | 1980-07-07 | 1985-01-10 | Nat Res Dev | Improvements in or relating to cell lines |
| DE3402927A1 (de) | 1984-01-28 | 1985-08-08 | Pfeifer & Langen, 5000 Köln | Zellkulturmikrotraeger, verfahren zu seiner herstellung und seine verwendung zum zuechten von verankerungsabhaengigen zellen |
| US5026650A (en) | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5631734A (en) | 1994-02-10 | 1997-05-20 | Affymetrix, Inc. | Method and apparatus for detection of fluorescently labeled materials |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US6214618B1 (en) | 1998-04-07 | 2001-04-10 | Solohill Engineering, Inc. | Microcarrier beads having a styrene copolymer core and a covalently linked tri-methylamine exterior |
| US6712610B2 (en) | 1999-04-02 | 2004-03-30 | Forsyth Dental Infirmary For Children | Characterization of an antibiotic impregnated delivery system as an intracanal medicament in endodontic therapy and method |
| CA2460206C (en) | 2001-10-02 | 2012-01-24 | Novo Nordisk Health Care Ag | Method for production of recombinant proteins in eukaryote cells |
| EP3023491A1 (en) * | 2002-03-15 | 2016-05-25 | University of North Carolina at Chapel Hill | Primitive and proximal hepatic stem cells |
| EP1496110B1 (en) | 2002-03-25 | 2012-05-09 | Hiroshima Industrial Promotion Organization | Method of proliferating human hepatocytes and method of obtaining human hepatocytes |
| WO2004056976A2 (en) | 2002-12-23 | 2004-07-08 | Fuji Photo Film B.V. | Process for coating cell-culture support |
| US7813977B2 (en) | 2003-09-11 | 2010-10-12 | Bgc Partners, Inc. | Graphical user interface related to non-standard trading of financial instruments |
| US7361493B1 (en) | 2004-05-26 | 2008-04-22 | The United States Of America As Represented By The Secretary Of The Department Of Veterans Affairs | Production of urokinase in a three-dimensional cell culture |
| JP2008514214A (ja) * | 2004-09-29 | 2008-05-08 | セルアーティス アーベー | ヒト胚盤胞由来幹細胞（ｈＢＳ）から肝細胞様細胞を発生させる方法 |
| EP1984488B1 (en) | 2006-01-30 | 2017-12-27 | University Of Virginia Patent Foundation | Methods of preparing and characterizing mesenchymal stem cell aggregates and uses thereof |
| WO2007127803A2 (en) | 2006-04-25 | 2007-11-08 | The Regents Of The University Of California | Administration of growth factors for the treatment of cns disorders |
| AU2007244965A1 (en) * | 2006-04-25 | 2007-11-08 | Artecel, Inc. | Adipose derived adult stem cells in hepatic regeneration |
| WO2007127454A2 (en) * | 2006-04-28 | 2007-11-08 | Cythera, Inc. | Hepatocyte lineage cells |
| CN101448527B (zh) * | 2006-05-19 | 2013-12-18 | 香港大学 | 细胞-基质微球，制备方法和应用 |
| US8158108B2 (en) | 2006-06-28 | 2012-04-17 | S.C. Johnson & Son, Inc. | VOC-free compressed gas aerosol compositions |
| US20080081064A1 (en) | 2006-09-28 | 2008-04-03 | Surmodics, Inc. | Implantable Medical Device with Apertures for Delivery of Bioactive Agents |
| WO2008144028A1 (en) * | 2007-05-14 | 2008-11-27 | The Johns Hopkins University | Methods for in vivo imaging of cells |
| US20100190202A1 (en) * | 2007-07-20 | 2010-07-29 | Cellartis Ab | Novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts stem cells |
| EP2245141B1 (en) | 2008-01-18 | 2018-03-14 | Regents of the University of Minnesota | Stem cell aggregates and methods for making and using |
| JP2011510750A (ja) | 2008-01-29 | 2011-04-07 | クライマン、ギルバート・エイチ | 薬物送達デバイス、キット及びそれらの方法 |
| US20100112031A1 (en) | 2008-10-17 | 2010-05-06 | University Of Virginia Patent Foundation | Compositions And Methods For Regulating Extracellular Matrix Production In Adipose Derived Cells |
| US20100098739A1 (en) | 2008-10-20 | 2010-04-22 | University Of Virginia Patent Foundation | Compositions and methods for modular soft tissue repair |
| WO2010049752A1 (en) * | 2008-10-31 | 2010-05-06 | Katholieke Universiteit Leuven | Optimized methods for differentiation of cells into cells with hepatocyte and hepatocyte progenitor phenotypes, cells produced by the methods, and methods for using the cells |
| US8895300B2 (en) * | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
| EP3260534A1 (en) | 2008-11-20 | 2017-12-27 | Janssen Biotech, Inc. | Pluripotent stem cell culture on micro-carriers |
| KR101017710B1 (ko) * | 2009-02-05 | 2011-02-25 | 한양대학교 산학협력단 | 세포 스페로이드 형태의 이식용 성체 줄기세포 집합체 및 그 제조방법 |
| US20120207744A1 (en) * | 2009-03-19 | 2012-08-16 | Mendlein John D | Reprogramming compositions and methods of using the same |
| JP5792714B2 (ja) | 2009-05-28 | 2015-10-14 | コーニング インコーポレイテッド | 細胞培養のための膨潤性の合成マイクロキャリア |
| JP2011013535A (ja) | 2009-07-03 | 2011-01-20 | Olympus Corp | 光調節装置及び光学系 |
| WO2011006205A1 (en) * | 2009-07-15 | 2011-01-20 | Regenertech Pty Limited | Method of producing progenitor cells from differentiated cells |
| CN101914495A (zh) | 2010-07-22 | 2010-12-15 | 吉林大学 | 用于体外大量扩增毛囊干细胞的培养方法 |
| ITMI20111096A1 (it) * | 2011-06-17 | 2012-12-18 | Filosa Stefania | Cellule dell'endoderma da cellule pluripotenti |
| US9215088B2 (en) | 2011-06-29 | 2015-12-15 | Broadcom Corporation | Identification of application sessions |
| JP6124347B2 (ja) * | 2011-08-02 | 2017-05-10 | 石川 哲也 | ＴＧＦ−βシグナリング阻害剤および／またはＹ−２７６３２による肝前駆細胞の誘導方法および製造方法、並びに当該肝前駆細胞から肝細胞の製造方法 |
| ES2600321T3 (es) * | 2011-08-04 | 2017-02-08 | Ethianum Betriebsgesellschaft Mbh & Co. Kg | Medios para la regeneración del hígado |
-
2014
- 2014-09-17 WO PCT/US2014/056049 patent/WO2015042125A2/en not_active Ceased
- 2014-09-17 US US14/917,558 patent/US10004767B2/en active Active
- 2014-09-17 EP EP19191062.9A patent/EP3685884A1/en not_active Withdrawn
- 2014-09-17 ES ES14846200T patent/ES2767062T3/es active Active
- 2014-09-17 JP JP2016543968A patent/JP6683615B2/ja active Active
- 2014-09-17 SG SG10201802231RA patent/SG10201802231RA/en unknown
- 2014-09-17 EP EP14846200.5A patent/EP3046628B1/en active Active
- 2014-09-17 SG SG10201914005TA patent/SG10201914005TA/en unknown
- 2014-09-17 SG SG11201707615YA patent/SG11201707615YA/en unknown
- 2014-09-17 CA CA2959117A patent/CA2959117C/en active Active
- 2014-09-17 DK DK14846200.5T patent/DK3046628T3/da active
-
2018
- 2018-05-08 US US15/974,377 patent/US11291691B2/en active Active
-
2020
- 2020-03-26 JP JP2020055548A patent/JP7057797B2/ja active Active
-
2022
- 2022-02-15 US US17/672,528 patent/US20220168359A1/en not_active Abandoned
- 2022-04-08 JP JP2022064376A patent/JP2022088668A/ja active Pending
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