SG10201914005TA - Methods and compositions for producing hepatocyte-like cells - Google Patents

Methods and compositions for producing hepatocyte-like cells

Info

Publication number
SG10201914005TA
SG10201914005TA SG10201914005TA SG10201914005TA SG10201914005TA SG 10201914005T A SG10201914005T A SG 10201914005TA SG 10201914005T A SG10201914005T A SG 10201914005TA SG 10201914005T A SG10201914005T A SG 10201914005TA SG 10201914005T A SG10201914005T A SG 10201914005TA
Authority
SG
Singapore
Prior art keywords
compositions
cells
methods
producing hepatocyte
hepatocyte
Prior art date
Application number
SG10201914005TA
Other languages
English (en)
Inventor
Gary Peltz
Dan Xu
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of SG10201914005TA publication Critical patent/SG10201914005TA/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62—DMSO
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1384—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from adipose-derived stem cells [ADSC], from adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Transplantation (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Physiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Cardiology (AREA)
  • Emergency Medicine (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Endocrinology (AREA)
SG10201914005TA 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells SG10201914005TA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361880056P 2013-09-19 2013-09-19

Publications (1)

Publication Number Publication Date
SG10201914005TA true SG10201914005TA (en) 2020-03-30

Family

ID=52689587

Family Applications (3)

Application Number Title Priority Date Filing Date
SG10201914005TA SG10201914005TA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells
SG10201802231RA SG10201802231RA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells
SG11201707615YA SG11201707615YA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells

Family Applications After (2)

Application Number Title Priority Date Filing Date
SG10201802231RA SG10201802231RA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells
SG11201707615YA SG11201707615YA (en) 2013-09-19 2014-09-17 Methods and compositions for producing hepatocyte-like cells

Country Status (8)

Country Link
US (3) US10004767B2 (cg-RX-API-DMAC7.html)
EP (2) EP3685884A1 (cg-RX-API-DMAC7.html)
JP (3) JP6683615B2 (cg-RX-API-DMAC7.html)
CA (1) CA2959117C (cg-RX-API-DMAC7.html)
DK (1) DK3046628T3 (cg-RX-API-DMAC7.html)
ES (1) ES2767062T3 (cg-RX-API-DMAC7.html)
SG (3) SG10201914005TA (cg-RX-API-DMAC7.html)
WO (1) WO2015042125A2 (cg-RX-API-DMAC7.html)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
JP2020536632A (ja) * 2017-10-05 2020-12-17 ザ・ジョンズ・ホプキンス・ユニバーシティ 移植可能なバイオリアクター、ならびにその作製方法および使用方法
CN109749981B (zh) * 2017-11-06 2023-10-10 博品(上海)生物医药科技有限公司 人源脂肪干细胞来源的肝细胞样细胞及其制备方法和应用
KR102857041B1 (ko) * 2018-09-20 2025-09-09 노바딥 바이오사이언시스 에스에이 지방 유래 줄기 세포 및 젤라틴을 포함하는 생체물질 및 이의 제조방법
BR112022018092A2 (pt) 2020-03-11 2022-11-22 Bit Bio Ltd Método de gerar células hepáticas
WO2023060186A1 (en) 2021-10-07 2023-04-13 Hepatx Corporation Methods of tracking donor cells in a recipient
US20230174949A1 (en) * 2021-12-03 2023-06-08 The Regents Of The University Of California Engineered vascularized organoids

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4491632A (en) 1979-10-22 1985-01-01 The Massachusetts General Hospital Process for producing antibodies to hepatitis virus and cell lines therefor
US4444887A (en) 1979-12-10 1984-04-24 Sloan-Kettering Institute Process for making human antibody producing B-lymphocytes
EP0043718B1 (en) 1980-07-07 1984-11-28 National Research Development Corporation Improvements in or relating to cell lines
DE3402927A1 (de) 1984-01-28 1985-08-08 Pfeifer & Langen, 5000 Köln Zellkulturmikrotraeger, verfahren zu seiner herstellung und seine verwendung zum zuechten von verankerungsabhaengigen zellen
US5026650A (en) 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US6214618B1 (en) 1998-04-07 2001-04-10 Solohill Engineering, Inc. Microcarrier beads having a styrene copolymer core and a covalently linked tri-methylamine exterior
US6712610B2 (en) 1999-04-02 2004-03-30 Forsyth Dental Infirmary For Children Characterization of an antibiotic impregnated delivery system as an intracanal medicament in endodontic therapy and method
JP4351049B2 (ja) 2001-10-02 2009-10-28 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 真核細胞中で組換えタンパク質を生産する方法
CA2479510C (en) * 2002-03-15 2019-10-29 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells
CN1643136A (zh) 2002-03-25 2005-07-20 独立行政法人科学技术振兴机构 人肝细胞增殖方法和人肝细胞的获取方法
WO2004056976A2 (en) 2002-12-23 2004-07-08 Fuji Photo Film B.V. Process for coating cell-culture support
US7813977B2 (en) 2003-09-11 2010-10-12 Bgc Partners, Inc. Graphical user interface related to non-standard trading of financial instruments
US7361493B1 (en) 2004-05-26 2008-04-22 The United States Of America As Represented By The Secretary Of The Department Of Veterans Affairs Production of urokinase in a three-dimensional cell culture
GB2434156B (en) * 2004-09-29 2010-06-30 Cellartis Ab Methods for the generation of hepatocyte-like cells from human blastocyst-derived stem (hBs)
EP1984488B1 (en) 2006-01-30 2017-12-27 University Of Virginia Patent Foundation Methods of preparing and characterizing mesenchymal stem cell aggregates and uses thereof
AU2007244826B2 (en) 2006-04-25 2013-04-11 The Regents Of The University Of California Administration of growth factors for the treatment of CNS disorders
AU2007244965A1 (en) * 2006-04-25 2007-11-08 Artecel, Inc. Adipose derived adult stem cells in hepatic regeneration
US7989204B2 (en) * 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
US8679809B2 (en) * 2006-05-19 2014-03-25 The University Of Hong Kong Cell-matrix microspheres, methods for preparation and applications
US8158108B2 (en) 2006-06-28 2012-04-17 S.C. Johnson & Son, Inc. VOC-free compressed gas aerosol compositions
US20080081064A1 (en) 2006-09-28 2008-04-03 Surmodics, Inc. Implantable Medical Device with Apertures for Delivery of Bioactive Agents
US20110020239A1 (en) * 2007-05-14 2011-01-27 The Johns Hopkins University Methods for in vivo imaging of cells
SG183067A1 (en) * 2007-07-20 2012-08-30 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells
WO2009092092A1 (en) 2008-01-18 2009-07-23 Regents Of The University Of Minnesota Stem cell aggregates and methods for making and using
WO2009097468A2 (en) 2008-01-29 2009-08-06 Kliman Gilbert H Drug delivery devices, kits and methods therefor
US20100112031A1 (en) 2008-10-17 2010-05-06 University Of Virginia Patent Foundation Compositions And Methods For Regulating Extracellular Matrix Production In Adipose Derived Cells
US20100098739A1 (en) 2008-10-20 2010-04-22 University Of Virginia Patent Foundation Compositions and methods for modular soft tissue repair
US9057051B2 (en) * 2008-10-31 2015-06-16 Katholieke Universiteit Leuven Optimized methods for differentiation of cells into cells with hepatocyte progenitor phenotypes, cells produced by the methods, and methods of using the cells
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
KR101774546B1 (ko) 2008-11-20 2017-09-04 얀센 바이오테크 인코포레이티드 마이크로-캐리어 상의 만능 줄기 세포 배양
KR101017710B1 (ko) * 2009-02-05 2011-02-25 한양대학교 산학협력단 세포 스페로이드 형태의 이식용 성체 줄기세포 집합체 및 그 제조방법
WO2010108126A2 (en) * 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Reprogramming compositions and methods of using the same
JP5792714B2 (ja) 2009-05-28 2015-10-14 コーニング インコーポレイテッド 細胞培養のための膨潤性の合成マイクロキャリア
JP2011013535A (ja) 2009-07-03 2011-01-20 Olympus Corp 光調節装置及び光学系
US20120282228A1 (en) * 2009-07-15 2012-11-08 Vishal Bhasin Method of producing progenitor cells from differentiated cells
CN101914495A (zh) 2010-07-22 2010-12-15 吉林大学 用于体外大量扩增毛囊干细胞的培养方法
ITMI20111096A1 (it) * 2011-06-17 2012-12-18 Filosa Stefania Cellule dell'endoderma da cellule pluripotenti
US9215088B2 (en) 2011-06-29 2015-12-15 Broadcom Corporation Identification of application sessions
WO2013018851A1 (ja) * 2011-08-02 2013-02-07 独立行政法人国立がん研究センター 誘導肝幹細胞から肝分化誘導する方法及び誘導肝前駆細胞
EP2554176B1 (en) * 2011-08-04 2016-07-27 ETHIANUM Betriebsgesellschaft mbH & Co. KG Means for liver regeneration

Also Published As

Publication number Publication date
SG11201707615YA (en) 2017-10-30
EP3046628A2 (en) 2016-07-27
CA2959117C (en) 2023-08-22
US11291691B2 (en) 2022-04-05
DK3046628T3 (da) 2020-02-10
US20160256499A1 (en) 2016-09-08
CA2959117A1 (en) 2015-03-26
JP2016529920A (ja) 2016-09-29
EP3685884A1 (en) 2020-07-29
JP2020114237A (ja) 2020-07-30
SG10201802231RA (en) 2018-05-30
ES2767062T3 (es) 2020-06-16
EP3046628B1 (en) 2019-11-06
WO2015042125A2 (en) 2015-03-26
JP2022088668A (ja) 2022-06-14
US20190083545A1 (en) 2019-03-21
US20220168359A1 (en) 2022-06-02
WO2015042125A3 (en) 2015-11-05
JP7057797B2 (ja) 2022-04-20
EP3046628A4 (en) 2017-03-08
US10004767B2 (en) 2018-06-26
JP6683615B2 (ja) 2020-04-22

Similar Documents

Publication Publication Date Title
IL289821A (en) Preparations and methods for immunotherapy
IL273205A (en) Preparations and methods
GB2533892B (en) Compositions and methods
SG10201706858XA (en) Methods and compositions for mobilizing stem cells
SG11201510177WA (en) SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
SMT201900635T1 (it) Composizioni e metodi di attivazione della segnalazione dipendente dallo "stimolatore di geni interferone"
SG11201507688VA (en) Compositions and methods for immunotherapy
GB201308072D0 (en) Compositions and methods
SG11201504260UA (en) Low acidic species compositions and methods for producing and using the same
EP2943591A4 (en) METHOD AND COMPOSITIONS FOR PROFILING SINGLE CELL EXPRESSION
ZA201506874B (en) Binder compositions and methods for making and using same
IL240125A0 (en) Methods for producing dicotpiperazines and compositions containing dicotpiperazines
IL243235A0 (en) Preparations and methods for immunotherapy
GB201305813D0 (en) Compositions and methods
SG10201914005TA (en) Methods and compositions for producing hepatocyte-like cells
ZA201506873B (en) Binder compositions and methods for making and using same
GB201322456D0 (en) Radiotracer compositions and methods
EP2964610A4 (en) VINYL SULFON-BASED 18F LABELING COMPOSITIONS AND METHOD AND USES THEREOF
GB201322617D0 (en) Methods and compositions
GB201312393D0 (en) Compositions and Methods
GB201308949D0 (en) Compositions and methods for reducing methane production
GB201319917D0 (en) Accelerator Compositions and Methods