JP2016511405A5 - - Google Patents
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- JP2016511405A5 JP2016511405A5 JP2015560136A JP2015560136A JP2016511405A5 JP 2016511405 A5 JP2016511405 A5 JP 2016511405A5 JP 2015560136 A JP2015560136 A JP 2015560136A JP 2015560136 A JP2015560136 A JP 2015560136A JP 2016511405 A5 JP2016511405 A5 JP 2016511405A5
- Authority
- JP
- Japan
- Prior art keywords
- receptor protein
- ligand
- cell
- organelle
- binding molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000027455 binding Effects 0.000 claims 17
- 239000003446 ligand Substances 0.000 claims 9
- 102000005962 receptors Human genes 0.000 claims 9
- 108020003175 receptors Proteins 0.000 claims 9
- 210000004027 cells Anatomy 0.000 claims 8
- 210000003463 Organelles Anatomy 0.000 claims 5
- 230000003993 interaction Effects 0.000 claims 3
- 230000003834 intracellular Effects 0.000 claims 3
- 230000014759 maintenance of location Effects 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000000717 retained Effects 0.000 claims 1
Claims (1)
- 細胞内で細胞表面に発現した受容体タンパク質とその同族リガンドの間の相互作用をブロック又は妨害する結合性分子を選択する方法であって、
前記細胞において、前記細胞の表面で発現可能な受容体タンパク質を発現させるステップ、
前記細胞において、前記受容体タンパク質の同族リガンドを発現させるステップであって、細胞内オルガネラにおける前記リガンドの保持、及び前記オルガネラにおける前記受容体タンパク質と前記同族リガンドの相互作用を可能にする条件下で、前記同族リガンドが、前記リガンドを細胞内オルガネラにおいて保持するための配列で分子的にタグ付けされているステップ、
前記細胞内オルガネラにおいて保持されている前記受容体タンパク質又は前記リガンドタンパク質のいずれかを特異的に結合する結合性分子又はその結合性断片若しくは部分を前記細胞に導入するステップ、及び
前記細胞表面で発現した前記受容体タンパク質のレベルを検出するステップを含み、
前記結合性分子又はその結合性断片若しくは部分が、前記オルガネラにおいて前記受容体と前記同族リガンドの結合をブロック又は妨害する場合、前記受容体タンパク質が、前記細胞表面で発現及び検出可能であり、前記受容体タンパク質とリガンドの相互作用をブロック又は妨害する前記結合性分子又はその結合性断片若しくは部分が選択可能である、前記方法。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361771562P | 2013-03-01 | 2013-03-01 | |
| US61/771,562 | 2013-03-01 | ||
| PCT/PT2014/000016 WO2014133405A2 (en) | 2013-03-01 | 2014-03-03 | Cell-based methods for coupling protein interactions and binding molecule selection and diversification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016511405A JP2016511405A (ja) | 2016-04-14 |
| JP2016511405A5 true JP2016511405A5 (ja) | 2017-04-06 |
Family
ID=50680097
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015560136A Pending JP2016511405A (ja) | 2013-03-01 | 2014-03-03 | 結合性タンパク質相互作用及び結合性分子の選択及び多様化のための細胞に基づく方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9487773B2 (ja) |
| EP (2) | EP2962103A2 (ja) |
| JP (1) | JP2016511405A (ja) |
| ES (1) | ES2872048T3 (ja) |
| PT (1) | PT3588089T (ja) |
| WO (1) | WO2014133405A2 (ja) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230155600A (ko) | 2014-04-03 | 2023-11-10 | 아이쥐엠 바이오사이언스 인코포레이티드 | 변형된 j-사슬 |
| KR102435324B1 (ko) | 2015-01-20 | 2022-08-23 | 아이쥐엠 바이오사이언스 인코포레이티드 | 종양 괴사 인자(ｔｎｆ) 수퍼패밀리 수용체 결합 분자 및 그의 용도 |
| ES2874558T3 (es) | 2015-03-04 | 2021-11-05 | Igm Biosciences Inc | Moléculas de unión a CD20 y usos de las mismas |
| AU2016329197B2 (en) | 2015-09-30 | 2021-01-21 | Igm Biosciences, Inc. | Binding molecules with modified J-chain |
| WO2017059387A1 (en) | 2015-09-30 | 2017-04-06 | Igm Biosciences, Inc. | Binding molecules with modified j-chain |
| GB201704115D0 (en) * | 2017-03-15 | 2017-04-26 | Oxford Genetics Ltd | Method of selecting for antibodies |
| CN113388638A (zh) * | 2021-04-13 | 2021-09-14 | 中国人民解放军西部战区总医院 | 一种同时结合TGFβ和VEGF的双靶点融合蛋白质粒的构建方法 |
| WO2023250481A2 (en) * | 2022-06-23 | 2023-12-28 | Fuller Laboratories | Rickettsia igm assay |
| WO2024081807A2 (en) * | 2022-10-12 | 2024-04-18 | Massachusetts Institute Of Technology | Library-scale methods for polypeptide functional analysis |
| CN116236584B (zh) * | 2023-02-20 | 2024-03-22 | 四川大学 | 一种用于高效递送siRNA的多糖-多肽偶联物 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3832847B2 (ja) * | 1992-07-17 | 2006-10-11 | ダナ−ファーバー キャンサー インスティテュート | 標的分子の細胞内結合方法 |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US7179462B2 (en) * | 2000-06-02 | 2007-02-20 | University Of Connecticut Health Center | α (2) macroglobulin receptor as a heat shock protein receptor and uses thereof |
| US20070248607A1 (en) * | 2005-11-03 | 2007-10-25 | Thomas Spies | Negative immunomodulation of immune responses by nkg2d-positive cd4+ cells |
| US9518254B2 (en) * | 2009-05-04 | 2016-12-13 | San Diego State University Research Foundation | Compositions and methods for identifying enzyme and transport protein inhibitors |
| AU2010249470B2 (en) * | 2009-05-20 | 2015-06-25 | Novimmune S.A. | Synthetic Polypeptide Libraries And Methods For Generating Naturally Diversified Polypeptide Variants |
-
2014
- 2014-02-28 US US14/193,679 patent/US9487773B2/en active Active
- 2014-03-03 JP JP2015560136A patent/JP2016511405A/ja active Pending
- 2014-03-03 ES ES19020049T patent/ES2872048T3/es active Active
- 2014-03-03 WO PCT/PT2014/000016 patent/WO2014133405A2/en active Application Filing
- 2014-03-03 PT PT190200493T patent/PT3588089T/pt unknown
- 2014-03-03 EP EP14722391.1A patent/EP2962103A2/en not_active Withdrawn
- 2014-03-03 EP EP19020049.3A patent/EP3588089B1/en active Active
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