JP2016050171A - Antioxidant derived from microalgae and cosmetics or external preparation for skin containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a safe and stable antioxidant derived from natural origin without a concern of organic solvent residues which can be contained in cosmetics or external preparations for skin or the like.SOLUTION: Safe and stable cosmetics or external preparations for skin can be provided readily by finding out excellent antioxidant ability of the oil soluble extract from one or more phycobionts selected from Stylonematales, Rhodosorus sp. or Rhodospora sp., Porphyridiales, or Porphyridium sp. cultivated under oxidative stress conditions to contain the same.SELECTED DRAWING: None

Description

  The present invention relates to an antioxidant comprising an oil-soluble extract of microalgae as an active ingredient, and a cosmetic or skin external preparation containing the antioxidant.

  In general, an external preparation for skin such as cosmetics and pharmaceuticals is blended with an antioxidant for the purpose of preventing oxidative deterioration due to oxygen dissolved in the air and in the product. In addition, due to the recent rise in natural taste and natural products, natural antioxidants are preferred.For example, vitamins such as vitamin C and vitamin E, flavonoids such as polyphenols and catechins are widely used in cosmetics and pharmaceuticals. It comes to be blended. However, although these naturally-derived antioxidants have high safety, they lack stability, and may cause discoloration and odor in the product, and are safe and stable naturally-occurring antioxidants. Development is demanded by the market.

  In addition, photoaging is caused by ultraviolet rays, and active oxygen species are generated in the skin, which causes rough skin, wrinkles, dullness, and the like. In order to prevent such skin oxidative stress, cosmetics or external preparations for skin containing antioxidants are used.

  Conventionally, microalgae have attracted attention as a biological source for obtaining antioxidant substances. For example, the green alga Chlorella (Patent Document 1), the cyanobacteria Nostock, Spirulina, Euglena Euglena (Patent Document 2), the diatom Eucampia, and Raphonice (Patent Document 3) are known. In addition, for the extraction of antioxidant substances from these algae, a method of extracting water-soluble components and oil-soluble components from seaweeds with a water-containing organic solvent such as ethanol (Patent Document 4), chlorella is extracted with an organic solvent, silica gel A method of fractionation by the method (Patent Document 5) is proposed. However, there existed problems such as residual organic solvents and unstable antioxidants obtained.

JP 2002-114703 A JP 2002-69443 A Patent No. 5499352 Japanese Patent Laid-Open No. 62-286907 JP-A-5-86796

  Provided is a safe and stable naturally-occurring antioxidant that can be easily blended into cosmetics or skin external preparations without worrying about residual organic solvents.

  As a result of intensive studies on a wide range of safe and stable naturally-occurring antioxidants as a solution to these problems, the present inventors have found that safe and stable naturally-derived antioxidants are extracted from oil-soluble extracts derived from specific microalgae. The present inventors have found an antioxidant substance, and found that an antioxidant containing this oil-soluble extract as an active ingredient can be easily blended into cosmetics or skin external preparations.

  That is, the present invention is one or more species selected from the genus Rhodosorus sp. Of the order Stylonematales, Rhodospora sp., Porphyridiales of Porphyridiales or Porphyridium sp. It is a safe and stable novel antioxidant comprising an oil-soluble extract from two or more algal bodies as an active ingredient.

  The antioxidant of this invention is excellent in stability in cosmetics or a skin external preparation, and has a safe antioxidant action. Therefore, the antioxidant containing the oil-soluble extract of the present invention can be easily blended into cosmetics or skin external preparations, and these exhibit excellent antioxidant action.

As the microalgae used in the present invention, there is a genus Rhodosols of the order of Stylonematales or a genus Rhodospora, a genus Polyphyridium of Porphyridiales,
More specifically, Stylonematales Rhodosols sp. (Rhodosorus sp.) Or Rhodospora sp. (Rhodospora sp.), Porphyridiales polyphylidium sp. (Porphyridium sp.) Is preferred, and marine Rhodosolus sp. (Rhodosorus sp.), Rhodosorus marinus or Rhodospora sordida, polyphylidium cruentum or polyphylidium purpleum are more preferred.

  As a method for culturing the microalgae, it is preferable that the microalgae is cultured under oxidative stress conditions such as supplying oxygen under sunlight.

  Examples of the extraction oil used in the present invention include vegetable oils, ester oils, and hydrocarbon oils, and oils that are liquid at room temperature are preferred. Specifically, olive oil, macadamia nut oil, almond oil, jojoba oil, sunflower oil and other vegetable oils, cetyl ethyl hexanoate, ethyl hexyl palmitate, glyceryl tri-2-ethyl hexanoate, tri (capryl / capric acid) glyceryl, myristic There are ester oils such as isopropyl acid, hydrocarbon oils such as squalane, liquid paraffin, and olefin oligomers. Of these, olive oil, macadamia nut oil, jojoba oil, sunflower oil, and squalane are more preferable.

  The method for producing the extract in the present invention is not particularly limited, for example, a method of extracting algae bodies dried after cultivation and pulverized in a conventional manner in oil, and algae bodies dried after cultivation are dispersed in oil. The conventional wet crushing method and ultrasonic crushing method, or the extraction method while crushing in a supercritical state or under high pressure.

  The algal body concentration at the time of extracting the extract from the algal body can be freely selected in the range of 1% by weight to 99% by weight depending on the use, but the range of 10% by weight to 50% by weight is more preferable. If the algal concentration is too high, the extraction efficiency is deteriorated, and if the concentration is too low, the antioxidant concentration in the extract is lowered. The antioxidant containing the oil-soluble extract becomes transparent by, for example, centrifuging the extract containing alga bodies and filtering the supernatant using a filter or the like.

  In the present invention, an antioxidant containing the oil-soluble extract of the microalga can be used by blending it with an external preparation for skin such as cosmetics or pharmaceuticals. Examples of cosmetics include creams, milky lotions, beauty essences, lotions, cleansings, lips, foundations, cheeks, eye shadows, mascaras, facial cleansers, shampoos, conditioners, massage agents, and the like. The oil-soluble extract itself may be used for hair care and body care. If it is a pharmaceutical, it is widely applicable to products, such as various ointments. Furthermore, the form which the antioxidant which contains the oil-soluble extract of this invention can take is not limited to these dosage forms and product forms.

  When the antioxidant containing the oil-soluble extract in the present invention as an active ingredient is blended in cosmetics or external preparations for skin, the blending amount is not particularly limited. For example, 0.01 to 99 mass with respect to the product. %.

Although the Example of this invention is described below, it is not limited to this.

  The microalgae listed in Tables 1 and 2 are added to seawater filtered through a membrane filter having a pore size of 0.45 μm, and there is oxidative stress (under sunlight and aeration) or no oxidative stress (under fluorescent light irradiation and no aeration). Culture was performed for 5 to 14 days under the conditions. The cultured microalgae were collected, and singlet oxygen scavenging activity was measured using extracts obtained by crushing and extracting in various solvents for 6 to 48 hours. The evaluation method is according to Kazuo Mukai et al., Development of Antioxidant Singlet Oxygen Scavenging Activity Evaluation Method (SOAC Method) of Food, Oreo Science, Vol. 13, No. 8, 371-378, and the extract is 15 mg / mL. The concentration was evaluated.

The results are shown in Tables 1 and 2. As the culture conditions, singlet oxygen scavenging activity ability excellent under the conditions in which oxidative stress was applied during the culture was confirmed. As for the extraction solvent, the singlet oxygen scavenging activity ability superior to the extraction with oily solvents such as squalane and jojoba oil was confirmed as compared with the extracts such as water and polyhydric alcohol.

In order to confirm the usefulness of the microalgal oily component extract to the skin, the inhibitory effect on the release of inflammatory factors due to the generation of excessive active oxygen in the cells of the skin was evaluated.
Using Quantikine IL-1α, Human, ELISA kit and Cayman Prostaglandin E2 Express EIA kit, ultraviolet rays (5 mJ / cm ² UVB) are irradiated to generate active oxygen in normal human epidermal cells and then release The inhibitory action of each microalgae oily component extract on the inflammatory marker is evaluated by ELISA.

The results are shown in Table 3. Compared with the extract with hydrous alcohol, the singlet oxygen surface activity ability superior to the extract with oil-based solvents such as squalane was confirmed.

Below, the example of application of the skin external preparation which mix | blended the microalga-derived antioxidant of this invention is shown. A compounding quantity is the mass%. In Examples 3 to 13, an anti-inflammatory effect was observed in all cases.

Essence mass%
Invention product 1 (Rodosolus sp. Extract) 0.1
Squalane 1.0
Behenyl alcohol 4.0
Vaseline 3.0
Liquid paraffin 15.0
Decaglyceryl monolaurate 1.0
Monostearic acid POE (20) sorbitan 3.0
Xanthan gum 0.1
1,3-butylene glycol 10.0
Preservative Appropriate amount Perfume Appropriate amount Purified water The remainder (preparation method) The oil-in-water emulsion composition was prepared according to a conventional method.

Toner lotion mass%
Invention product 2 (Rhodosolus marinus extract) 1.0
Invention product 3 (Rodospora sp. Extract) 1.0
ε-aminocaproic acid 0.1
L-ascorbic acid-2-magnesium phosphate 0.1
Chamomile extract 0.1
Condor crisp extract 0.1
Perilla extract 0.1
Squalane 0.2
Decaglyceryl monolaurate 2.0
Sodium hyaluronate 0.2
1,3-butylene glycol 3.0
Ethyl alcohol 10.0
Preservative Appropriate amount Perfume Appropriate amount Purified water The remainder (preparation method) All components were mixed at 50 ° C. until uniform, and prepared.

Cream 2 (emollient type)
Invention product 4 (Rodospora soridida extract) 0.5
Invention product 5 (polyphylidium cruentum extract) 0.5
Ascorbyl tetrahexyldecanoate 1.0
Trihexyldecanoic acid pyridoxine 0.5
dl-α-tocopherol 0.2
Retinol palmitate 0.1
Hydrogenated retinol 0.1
Squalane 10.0
Isocetyl myristate 6.0
Glyceryl tri-2-ethylhexanoate 3.0
Macadamia nut oil 1.0
Dimethylpolysiloxane 0.2
Cetanol 5.0
POE (20) cetyl ether 1.0
Tetraoleic acid POE (40) Sorbit 0.5
Glyceryl monostearate 1.0
Hydrogenated soybean lecithin 0.2
1,3-butylene glycol 5.0
Xanthan gum 0.1
Sodium hyaluronate 0.01
Citric acid 0.1
Sodium citrate 0.4
Preservative Appropriate amount Purified water The remainder (preparation method) Prepared according to a conventional method for producing an oil-in-water emulsion composition.

Beauty oil
Invention product 6 (Rodosols SP. And
Rhodospora soridida mixed extract) 1.0
Isocetyl myristate 10.0
Jojoba oil 5.0
Grape seed oil 1.0
Natural vitamin E 0.1
Retinol linoleate 0.1
Oil-soluble licorice extract 0.1
Squalane The remainder (preparation method) All components were mixed at 50 ° C. until uniform and prepared.

Skin lotion mass%
Tri (caprylic acid / capric acid) glyceryl 0.1
POP (4) POE (20) cetyl ether 0.6
Propylene glycol 10.0
Invention product 7 (Rodosolus sp. Extract) 0.5
Invention product 8 (Rodosolus sp. Extract) 0.5
Invention product 12 (with Rhodosolus Marinus
Rhodospora SP. Mixed extract) 0.5
Dipotassium glycyrrhetinate 0.2
Gigalchinasterata extract 0.2
Grape extract 0.001
Ergothioneine 0.001
Sodium hyaluronate 0.1
Preservative Appropriate amount Purified water The remainder (preparation method) Prepared according to a conventional method for producing an oil-in-water emulsion composition.

Milk mass%
d-δ-tocopherol 0.1
Invention product 9 (Rhodospora marinus extract) 1.0
Squalane 5.0
Cetyl 2-ethylhexanoate 5.0
Dimethylpolysiloxane 0.5
Cetyl palmitate 0.5
Behenyl alcohol 1.5
Stearic acid 0.5
Ceramide 2 0.1
Kimyl alcohol 0.1
Lipophilic glyceryl monostearate 1.0
Monostearic acid POE (20) sorbitan 1.0
Tetraoleic acid POE (40) sorbitan 1.5
Propylene glycol 7.0
Betula Pendula Extract 5.0
Xanthan gum 0.1
Preservative Appropriate amount Purified water The remainder (preparation method) Prepared according to a conventional method for producing an oil-in-water emulsion composition.

Cream 3 (water-in-oil emollient type)
Invention product 10 (Rhodosolus marinus extract) 1.0
cetyl ε-aminocaproate 0.1
Zinc glycine complex 0.1
Dimethicone copolyol 0.5
Decamethylcyclopentasiloxane 5.0
Tri (capryl / capric acid) glyceryl 15.0
Beeswax 2.0
Decaglyceryl pentahydroxy acid 2.0
Isostearic acid 1.0
Glycerin 4.5
Sodium hyaluronate 0.01
Preservative Appropriate amount Purified water The remainder (preparation method) An emulsified composition was prepared according to a conventional method for emulsifying water-in-oil type emulsified compositions.

Sunscreen cream
Invention product 1 (Rodosols sp .. extract) 0.5
Invention product 11 (Rodosolus sp. Extract) 0.5
Invention product 13 (Rodosolus sp. And Polyphylidium sp. Mixed extract) 0.5
Liquid paraffin 7.0
Decamethylcyclopentasiloxane 3.0
Cetyl alcohol 4.0
Condensed ricinoleic acid hexaglyceryl 0.5
POE (20) cetyl ether 1.0
Octyl paramethoxycinnamate 7.0
Titanium oxide 3.0
Sodium cetyl sulfate 1.0
Stearoyl methyl taurine sodium 0.3
1,3-butylene glycol 5.0
Xanthan gum 0.3
Sodium pyrrolidonecarboxylate 0.1
Camellia extract 0.1
Horsetail extract 0.1
Rosemary extract 0.1
Preservative Appropriate amount Purified water The remainder (preparation method) After the powder phase was added to the oil phase, an emulsion composition was prepared according to a conventional method for emulsifying water-in-oil emulsion compositions.

External preparation (ointment preparation)
Invention product 1 (Rodosolus sp. Extract) 10.0
Glyceryl monostearate 10.0
Liquid paraffin 10.0
Cetanol 6.0
White petrolatum 5.0
POE (30) cetyl ether 2.0
Propylene glycol 10.0
Preservative Appropriate amount Purified water The remainder (preparation method) An emulsified composition was prepared according to a conventional method for producing an ointment composition.

External preparation (emulsion)
Invention product 11 (Rodosolus sp. Extract) 15.0
White petrolatum 41.0
Lanolin 10.0
Microcrystalline wax 3.0
Sorbitan monooleate 4.75
Monooleic acid POE (20) sorbitan 0.25
Preservative Appropriate amount Purified water Remainder (Preparation method) An emulsion composition was prepared according to a conventional method for producing an emulsion composition.

External preparation (beauty oil)
Invention product 2 (Rhodosolus marinus extract) 99.0
Fragrance Appropriate amount Squalane The remainder (preparation method) Crushed in a conventional manner in squalane and filtered.

By extracting oils from microalgae cultured under specific conditions, there is no concern about residual organic solvents, and it can be easily blended into cosmetics or external skin preparations. Can provide.

Claims (2)

1 selected from Stylonematales (Rhodosorus sp.), Rhodospora sp., Porphyridiales (Porphyridium sp.) Cultured under oxidative stress conditions Antioxidants containing oil extracts of seeds or two or more algal bodies as active ingredients. A cosmetic or external preparation for skin comprising the antioxidant according to claim 1.