JP2015531407A5 - - Google Patents
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- Publication number
- JP2015531407A5 JP2015531407A5 JP2015536826A JP2015536826A JP2015531407A5 JP 2015531407 A5 JP2015531407 A5 JP 2015531407A5 JP 2015536826 A JP2015536826 A JP 2015536826A JP 2015536826 A JP2015536826 A JP 2015536826A JP 2015531407 A5 JP2015531407 A5 JP 2015531407A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- cell
- modified
- ompt1
- amino acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 102000004169 proteins and genes Human genes 0.000 claims description 92
- 108090000623 proteins and genes Proteins 0.000 claims description 92
- 235000018102 proteins Nutrition 0.000 claims description 78
- 150000001413 amino acids Chemical class 0.000 claims description 51
- 235000001014 amino acid Nutrition 0.000 claims description 45
- 229940024606 amino acid Drugs 0.000 claims description 45
- 230000001580 bacterial effect Effects 0.000 claims description 35
- 238000000034 method Methods 0.000 claims description 30
- 230000015572 biosynthetic process Effects 0.000 claims description 26
- 238000003786 synthesis reaction Methods 0.000 claims description 26
- 239000000284 extract Substances 0.000 claims description 17
- 241000894006 Bacteria Species 0.000 claims description 15
- 108020005038 Terminator Codon Proteins 0.000 claims description 12
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 150000007523 nucleic acids Chemical class 0.000 claims description 12
- 101710116435 Outer membrane protein Proteins 0.000 claims description 11
- 241000588724 Escherichia coli Species 0.000 claims description 7
- 230000010627 oxidative phosphorylation Effects 0.000 claims description 6
- 102000003712 Complement factor B Human genes 0.000 claims description 4
- 108090000056 Complement factor B Proteins 0.000 claims description 4
- 102000003960 Ligases Human genes 0.000 claims description 4
- 108090000364 Ligases Proteins 0.000 claims description 4
- 230000000295 complement effect Effects 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000011541 reaction mixture Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 230000002934 lysing effect Effects 0.000 claims description 3
- 239000004475 Arginine Substances 0.000 claims description 2
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims description 2
- 108010049152 Cold Shock Proteins and Peptides Proteins 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 102000017278 Glutaredoxin Human genes 0.000 claims description 2
- 108050005205 Glutaredoxin Proteins 0.000 claims description 2
- 108010063907 Glutathione Reductase Proteins 0.000 claims description 2
- 102100036442 Glutathione reductase, mitochondrial Human genes 0.000 claims description 2
- 239000004472 Lysine Substances 0.000 claims description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 239000004365 Protease Substances 0.000 claims description 2
- 102000001253 Protein Kinase Human genes 0.000 claims description 2
- 102000013090 Thioredoxin-Disulfide Reductase Human genes 0.000 claims description 2
- 108010079911 Thioredoxin-disulfide reductase Proteins 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 229960001230 asparagine Drugs 0.000 claims description 2
- 235000009582 asparagine Nutrition 0.000 claims description 2
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 claims description 2
- 230000006037 cell lysis Effects 0.000 claims description 2
- 238000003776 cleavage reaction Methods 0.000 claims description 2
- 230000000593 degrading effect Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 239000006166 lysate Substances 0.000 claims description 2
- 102000035118 modified proteins Human genes 0.000 claims description 2
- 108091005573 modified proteins Proteins 0.000 claims description 2
- 108020000161 polyphosphate kinase Proteins 0.000 claims description 2
- 238000001243 protein synthesis Methods 0.000 claims description 2
- 230000007017 scission Effects 0.000 claims description 2
- 230000014616 translation Effects 0.000 claims description 2
- -1 RF1 Proteins 0.000 claims 2
- 125000000539 amino acid group Chemical group 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 239000003488 releasing hormone Substances 0.000 claims 2
- 238000010025 steaming Methods 0.000 claims 2
- 102000035195 Peptidases Human genes 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000004090 dissolution Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 238000000338 in vitro Methods 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261713245P | 2012-10-12 | 2012-10-12 | |
| US61/713,245 | 2012-10-12 | ||
| PCT/US2013/063804 WO2014058830A1 (en) | 2012-10-12 | 2013-10-08 | Proteolytic inactivation of select proteins in bacterial extracts for improved expression |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017229231A Division JP2018029621A (ja) | 2012-10-12 | 2017-11-29 | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015531407A JP2015531407A (ja) | 2015-11-02 |
| JP2015531407A5 true JP2015531407A5 (https=) | 2016-11-24 |
| JP6421122B2 JP6421122B2 (ja) | 2018-11-07 |
Family
ID=49447839
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015536826A Active JP6421122B2 (ja) | 2012-10-12 | 2013-10-08 | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
| JP2017229231A Withdrawn JP2018029621A (ja) | 2012-10-12 | 2017-11-29 | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
| JP2020173282A Withdrawn JP2021003134A (ja) | 2012-10-12 | 2020-10-14 | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017229231A Withdrawn JP2018029621A (ja) | 2012-10-12 | 2017-11-29 | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
| JP2020173282A Withdrawn JP2021003134A (ja) | 2012-10-12 | 2020-10-14 | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US9650621B2 (https=) |
| EP (1) | EP2906592B1 (https=) |
| JP (3) | JP6421122B2 (https=) |
| KR (1) | KR102018863B1 (https=) |
| CN (1) | CN104837863B (https=) |
| AU (1) | AU2013329464C1 (https=) |
| CA (1) | CA2887355C (https=) |
| DK (1) | DK2906592T3 (https=) |
| ES (1) | ES2694683T3 (https=) |
| HU (1) | HUE041721T2 (https=) |
| IL (1) | IL238095B (https=) |
| PL (1) | PL2906592T3 (https=) |
| SG (1) | SG11201502875VA (https=) |
| WO (1) | WO2014058830A1 (https=) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6421122B2 (ja) | 2012-10-12 | 2018-11-07 | ストロ バイオファーマ， インコーポレイテッド | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
| CA2909516C (en) * | 2013-04-19 | 2023-03-07 | Sutro Biopharma, Inc. | Expression of biologically active proteins in a bacterial cell-free synthesis system using cell extracts with elevated levels of exogenous chaperones |
| US10316322B2 (en) * | 2014-07-02 | 2019-06-11 | Sutro Biopharma, Inc. | High growth capacity auxotrophic Escherichia coli and methods of use |
| EP3882343A3 (en) * | 2016-01-13 | 2021-12-08 | New England Biolabs, Inc. | Thermostable variants of t7 rna polymerase |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| CN106801047A (zh) * | 2016-12-30 | 2017-06-06 | 武汉金开瑞生物工程有限公司 | 一种通过无细胞蛋白合成系统制备半胱氨酸蛋白酶的方法 |
| ES3024474T3 (en) | 2016-12-30 | 2025-06-04 | Vaxcyte Inc | Polypeptide-antigen conjugates with non-natural amino acids |
| AU2018300069C1 (en) | 2017-07-11 | 2025-11-20 | Synthorx, Inc. | Incorporation of unnatural nucleotides and methods thereof |
| WO2019028419A1 (en) | 2017-08-03 | 2019-02-07 | Synthorx, Inc. | CYTOKINE CONJUGATES FOR THE TREATMENT OF PROLIFERATIVE AND INFECTIOUS DISEASES |
| SG11202003147XA (en) | 2017-10-12 | 2020-05-28 | Vaxcyte Inc | Periodontitis vaccine and related compositions and method of use |
| CA3091857A1 (en) | 2018-02-26 | 2019-08-29 | Synthorx, Inc. | Il-15 conjugates and uses thereof |
| WO2020014543A2 (en) | 2018-07-11 | 2020-01-16 | Actym Therapeutics, Inc. | Engineered immunostimulatory bacterial strains and uses thereof |
| CN108949796A (zh) * | 2018-07-27 | 2018-12-07 | 张家港市华天药业有限公司 | 一种用于合成谷胱甘肽的重组菌及谷胱甘肽的合成方法 |
| CN109055291A (zh) * | 2018-07-31 | 2018-12-21 | 张家港市华天药业有限公司 | 用于合成谷胱甘肽的重组菌及谷胱甘肽的合成方法 |
| ES3059360T3 (en) | 2018-11-08 | 2026-03-19 | Synthorx Inc | Interleukin 10 conjugates and uses thereof |
| KR20260039812A (ko) | 2019-02-06 | 2026-03-20 | 신톡스, 인크. | Il-2 콘쥬게이트 및 이의 사용 방법 |
| WO2020205584A1 (en) | 2019-04-02 | 2020-10-08 | Vaxcyte, Inc. | Optimized cell-free synthesis of invasion plasmid antigen b and related compositions and methods of use |
| CN114555128A (zh) | 2019-08-15 | 2022-05-27 | 新索思股份有限公司 | 采用il-2缀合物的免疫肿瘤学组合疗法 |
| MX2022002740A (es) | 2019-09-10 | 2022-03-25 | Synthorx Inc | Conjugados de il-2 y metodos de uso para tratar enfermedades autoinmunes. |
| IL291786A (en) | 2019-11-04 | 2022-06-01 | Synthorx Inc | Interleukin 10 conjugates and their use |
| US12351850B2 (en) * | 2020-04-30 | 2025-07-08 | Sutro Biopharma, Inc. | Methods of producing full-length antibodies using E. coli |
| JP2023531509A (ja) | 2020-06-25 | 2023-07-24 | シンソークス， インコーポレイテッド | Ｉｌ－２コンジュゲートおよび抗ｅｇｆｒ抗体を用いる免疫腫瘍学併用療法 |
| CA3192331A1 (en) | 2020-09-14 | 2022-03-17 | Sutro Biopharma, Inc. | Method for large scale production of antibodies using a cell-free protein synthesis system |
| WO2022076859A1 (en) | 2020-10-09 | 2022-04-14 | Synthorx, Inc. | Immuno oncology therapies with il-2 conjugates |
| AU2021356693A1 (en) | 2020-10-09 | 2023-06-15 | Synthorx, Inc. | Immuno oncology combination therapy with il-2 conjugates and pembrolizumab |
| EP4291243A1 (en) | 2021-02-12 | 2023-12-20 | Synthorx, Inc. | Lung cancer combination therapy with il-2 conjugates and an anti-pd-1 antibody or antigen-binding fragment thereof |
| TW202245843A (zh) | 2021-02-12 | 2022-12-01 | 美商欣爍克斯公司 | Ｉｌ－２接合物及西米普利單抗（ｃｅｍｉｐｌｉｍａｂ）之皮膚癌組合療法 |
| CN113322267B (zh) * | 2021-03-15 | 2024-01-05 | 天津大学 | 系统在提高非天然氨基酸的插入效率中的应用 |
| EP4346904A1 (en) | 2021-06-03 | 2024-04-10 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and cetuximab |
| US20250302950A1 (en) | 2021-12-20 | 2025-10-02 | Synthorx, Inc. | Head and neck cancer combination therapy comprising an il-2 conjugate and pembrolizumab |
| WO2023122750A1 (en) | 2021-12-23 | 2023-06-29 | Synthorx, Inc. | Cancer combination therapy with il-2 conjugates and cetuximab |
| WO2024136899A1 (en) | 2022-12-21 | 2024-06-27 | Synthorx, Inc. | Cancer therapy with il-2 conjugates and chimeric antigen receptor therapies |
| TW202444349A (zh) | 2023-03-20 | 2024-11-16 | 美商欣爍克斯公司 | 使用ｉｌ－２綴合物之癌症療法 |
| WO2025015041A1 (en) * | 2023-07-12 | 2025-01-16 | University Of Washington | Rna-only, positive control target for clinical diagnostic testing |
| WO2025158385A1 (en) | 2024-01-25 | 2025-07-31 | Genzyme Corporation | Pegylated il-2 for suppressing adaptive immune response to gene therapy |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100445394C (zh) * | 1999-03-04 | 2008-12-24 | 阿斯比奥制药株式会社 | 控制OmpT蛋白酶切割的方法 |
| DE60226411D1 (de) | 2001-01-25 | 2008-06-19 | Anthony C Forster | Verfahren und zusammensetzungen für peptid-, protein- und peptidomimetische synthese |
| ATE445699T1 (de) | 2002-08-19 | 2009-10-15 | Univ Leland Stanford Junior | Verbesserte verfahren zur in-vitro- proteinsynthese |
| DE10336705A1 (de) * | 2003-08-06 | 2005-03-10 | Rina Netzwerk Rna Technologien | Verfahren zur Herstellung eines Lysats zur zellfreien Proteinbiosynthese |
| AU2004276687B2 (en) * | 2003-09-30 | 2011-07-14 | Asubio Pharma Co., Ltd. | Method of cleaving polypeptide by using OmpT protease mutant |
| WO2006082059A1 (en) * | 2005-02-02 | 2006-08-10 | Universität Bayreuth | Esterases for monitoring protein biosynthesis in vitro |
| US8367588B2 (en) | 2005-10-12 | 2013-02-05 | The Scripps Research Institute | Selective posttranslational modification of phage-displayed polypeptides |
| KR20080113226A (ko) | 2006-03-16 | 2008-12-29 | 더 스크립스 리서치 인스티튜트 | 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현 |
| CN102348807B (zh) | 2009-01-12 | 2015-04-15 | 苏特罗生物制药公司 | 利用体外蛋白合成体系将非天然氨基酸引入蛋白的单装载体系 |
| JP6421122B2 (ja) | 2012-10-12 | 2018-11-07 | ストロ バイオファーマ， インコーポレイテッド | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
-
2013
- 2013-10-08 JP JP2015536826A patent/JP6421122B2/ja active Active
- 2013-10-08 AU AU2013329464A patent/AU2013329464C1/en active Active
- 2013-10-08 DK DK13779699.1T patent/DK2906592T3/en active
- 2013-10-08 WO PCT/US2013/063804 patent/WO2014058830A1/en not_active Ceased
- 2013-10-08 CA CA2887355A patent/CA2887355C/en active Active
- 2013-10-08 KR KR1020157012291A patent/KR102018863B1/ko active Active
- 2013-10-08 US US14/434,391 patent/US9650621B2/en active Active
- 2013-10-08 ES ES13779699.1T patent/ES2694683T3/es active Active
- 2013-10-08 SG SG11201502875VA patent/SG11201502875VA/en unknown
- 2013-10-08 CN CN201380063443.9A patent/CN104837863B/zh active Active
- 2013-10-08 HU HUE13779699A patent/HUE041721T2/hu unknown
- 2013-10-08 EP EP13779699.1A patent/EP2906592B1/en active Active
- 2013-10-08 PL PL13779699T patent/PL2906592T3/pl unknown
-
2015
- 2015-04-01 IL IL23809515A patent/IL238095B/en active IP Right Grant
-
2017
- 2017-04-10 US US15/483,766 patent/US10450353B2/en active Active
- 2017-11-29 JP JP2017229231A patent/JP2018029621A/ja not_active Withdrawn
-
2019
- 2019-09-06 US US16/562,837 patent/US11261219B2/en active Active
-
2020
- 2020-10-14 JP JP2020173282A patent/JP2021003134A/ja not_active Withdrawn
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