JP2015514212A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015514212A5 JP2015514212A5 JP2015503378A JP2015503378A JP2015514212A5 JP 2015514212 A5 JP2015514212 A5 JP 2015514212A5 JP 2015503378 A JP2015503378 A JP 2015503378A JP 2015503378 A JP2015503378 A JP 2015503378A JP 2015514212 A5 JP2015514212 A5 JP 2015514212A5
- Authority
- JP
- Japan
- Prior art keywords
- image
- signal
- sample
- generating
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 41
- 239000000523 sample Substances 0.000 claims description 36
- 239000003550 marker Substances 0.000 claims description 25
- 239000011230 binding agent Substances 0.000 claims description 20
- 239000002131 composite material Substances 0.000 claims description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 18
- 102000004169 proteins and genes Human genes 0.000 claims description 17
- 239000012472 biological sample Substances 0.000 claims description 16
- 150000007523 nucleic acids Chemical group 0.000 claims description 14
- 238000010186 staining Methods 0.000 claims description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
- 230000000877 morphologic effect Effects 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000001917 fluorescence detection Methods 0.000 claims 3
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000004043 dyeing Methods 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 238000007850 in situ PCR Methods 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 230000037452 priming Effects 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- HSTOKWSFWGCZMH-UHFFFAOYSA-N 3,3'-diaminobenzidine Chemical compound C1=C(N)C(N)=CC=C1C1=CC=C(N)C(N)=C1 HSTOKWSFWGCZMH-UHFFFAOYSA-N 0.000 description 4
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 238000002073 fluorescence micrograph Methods 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 3
- 238000013507 mapping Methods 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 101100239628 Danio rerio myca gene Proteins 0.000 description 1
- 101150029707 ERBB2 gene Proteins 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 101000782132 Homo sapiens Zinc finger protein 217 Proteins 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102100036595 Zinc finger protein 217 Human genes 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261618132P | 2012-03-30 | 2012-03-30 | |
| US61/618,132 | 2012-03-30 | ||
| PCT/US2013/033249 WO2013148448A1 (en) | 2012-03-30 | 2013-03-21 | Immunoflu0rescence and fluorescent-based nucleic acid analysis on a single sample |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015514212A JP2015514212A (ja) | 2015-05-18 |
| JP2015514212A5 true JP2015514212A5 (enExample) | 2017-06-29 |
| JP6225163B2 JP6225163B2 (ja) | 2017-11-01 |
Family
ID=48050953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015503378A Active JP6225163B2 (ja) | 2012-03-30 | 2013-03-21 | 単一試料での免疫蛍光及び蛍光系核酸分析 |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20150065371A1 (enExample) |
| EP (1) | EP2831591B1 (enExample) |
| JP (1) | JP6225163B2 (enExample) |
| KR (1) | KR20140142715A (enExample) |
| CN (1) | CN104520713A (enExample) |
| AU (1) | AU2013240127B2 (enExample) |
| CA (1) | CA2867508C (enExample) |
| IN (1) | IN2014DN07531A (enExample) |
| NZ (1) | NZ630091A (enExample) |
| RU (1) | RU2014137129A (enExample) |
| SG (1) | SG11201406105UA (enExample) |
| WO (1) | WO2013148448A1 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| JP6197039B2 (ja) | 2012-08-24 | 2017-09-13 | イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔｙ | ハイスループット多重検出用システム、装置及び方法 |
| US10088658B2 (en) | 2013-03-18 | 2018-10-02 | General Electric Company | Referencing in multi-acquisition slide imaging |
| ES2701092T5 (es) | 2014-02-24 | 2022-05-12 | Ventana Med Syst Inc | Procedimientos, kits y sistemas para puntuar la respuesta inmunitaria al cáncer por detección simultánea de CD3, CD8, CD20 y FOXP3 |
| CN104980558B (zh) * | 2014-04-01 | 2019-01-01 | 中国移动通信集团公司 | 一种信息处理的方法、装置、手机宠物客户端及移动终端 |
| WO2015157246A2 (en) * | 2014-04-07 | 2015-10-15 | Massachusetts Institute Of Technology | Use of microparticle additives to simultaneously enable artifact-free image registration, auto-focusing, and chromatic aberration correction in microscopy |
| WO2016026038A1 (en) * | 2014-08-18 | 2016-02-25 | Viewsiq Inc. | System and method for embedded images in large field-of-view microscopic scans |
| WO2016090148A1 (en) | 2014-12-03 | 2016-06-09 | IsoPlexis Corporation | Analysis and screening of cell secretion profiles |
| US11353448B2 (en) | 2015-02-13 | 2022-06-07 | California Institute Of Technology | Methods and compositions for quantifying metabolites and proteins from single cells |
| WO2017012555A1 (zh) * | 2015-07-20 | 2017-01-26 | 徐敏 | 光子结构和化学计量学病理系统 |
| WO2017110974A1 (ja) * | 2015-12-24 | 2017-06-29 | コニカミノルタ株式会社 | 画像処理装置及びプログラム |
| CN107419001A (zh) * | 2016-05-23 | 2017-12-01 | 益善生物技术股份有限公司 | Brca1、pten基因异常检测试剂盒及检测方法 |
| JP6231709B1 (ja) * | 2016-05-31 | 2017-11-15 | シスメックス株式会社 | 蛍光画像分析装置および分析方法 |
| EP3469548B1 (en) * | 2016-06-10 | 2020-05-13 | H. Hoffnabb-La Roche Ag | System for bright field image simulation |
| CN106290878A (zh) * | 2016-09-10 | 2017-01-04 | 天津大学 | 基于量子点的胰腺癌肿瘤标志物ca242免疫层析试纸条的制备方法 |
| WO2018049418A1 (en) | 2016-09-12 | 2018-03-15 | IsoPlexis Corporation | System and methods for multiplexed analysis of cellular and other immunotherapeutics |
| KR102638006B1 (ko) * | 2016-09-26 | 2024-02-20 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| EP4036578A1 (en) | 2016-11-11 | 2022-08-03 | Isoplexis Corporation | Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells |
| CN110226084A (zh) | 2016-11-22 | 2019-09-10 | 伊索普莱克西斯公司 | 用于细胞捕获的系统、装置和方法，以及其制造方法 |
| JP7116175B2 (ja) * | 2018-08-22 | 2022-08-09 | オリンパス株式会社 | 画像処理装置、撮像システム、画像処理装置の作動方法、及び画像処理装置の作動プログラム |
| US10921223B2 (en) * | 2018-10-12 | 2021-02-16 | Shenzhen Prs Limited | Process record slide for staining and method of using the same |
| CN110498858B (zh) * | 2019-07-26 | 2024-01-23 | 深圳市达科为生物工程有限公司 | 一种动态检测单细胞外泌蛋白分泌情况的方法 |
| WO2021127019A1 (en) * | 2019-12-17 | 2021-06-24 | Applied Materials, Inc. | System and method for acquisition and processing of multiplexed fluorescence in-situ hybridization images |
| EP4147044A4 (en) * | 2020-05-07 | 2024-06-12 | Advanced Cell Diagnostics, Inc. | METHOD FOR THE SIMULTANEOUS DETECTION OF TARGET NUCLEIC ACIDS AND PROTEINS AND KIT THEREFOR |
| AU2021314130A1 (en) * | 2020-07-20 | 2023-02-23 | Akoya Biosciences, Inc. | Processing and imaging tissue samples |
| US12400461B2 (en) | 2020-08-13 | 2025-08-26 | Ventana Medical Systems, Inc. | Automated identification of necrotic regions in digital images of multiplex immunofluorescence stained tissue |
| ES2993269T3 (en) * | 2020-09-18 | 2024-12-26 | 10X Genomics Inc | Sample handling apparatus and image registration methods |
| CN112415198B (zh) * | 2020-11-20 | 2022-11-11 | 四川大学华西医院 | Gp1bb检测试剂在制备肺癌筛查试剂盒中的用途 |
| CN113310963B (zh) * | 2021-05-31 | 2023-12-01 | 四川大学华西医院 | 一种改进的中性粒细胞NETs免疫荧光检测方法 |
| EP4433515A1 (en) | 2021-11-16 | 2024-09-25 | SOTIO Biotech Inc. | Treatment of myxoid/round cell liposarcoma patients |
| EP4469787A1 (en) * | 2022-01-28 | 2024-12-04 | The University Of Southern California | Liquid biopsy analytes to define cancer stages |
| WO2023147139A1 (en) * | 2022-01-28 | 2023-08-03 | University Of Southern California | Identifying non-disease patients using a disease related assay and analysis in the liquid biopsy |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6404906B2 (en) | 1997-03-03 | 2002-06-11 | Bacus Research Laboratories,Inc. | Method and apparatus for acquiring and reconstructing magnified specimen images from a computer-controlled microscope |
| US6993169B2 (en) * | 2001-01-11 | 2006-01-31 | Trestle Corporation | System and method for finding regions of interest for microscopic digital montage imaging |
| US7629125B2 (en) | 2006-11-16 | 2009-12-08 | General Electric Company | Sequential analysis of biological samples |
| US20110189670A1 (en) * | 2008-07-07 | 2011-08-04 | Ruth L Katz | Circulating Tumor and Tumor Stem Cell Detection Using Genomic Specific Probes |
| US8269827B2 (en) * | 2009-09-29 | 2012-09-18 | General Electric Company | System and methods for mapping fluorescent images into a bright field color space |
-
2013
- 2013-03-21 WO PCT/US2013/033249 patent/WO2013148448A1/en not_active Ceased
- 2013-03-21 SG SG11201406105UA patent/SG11201406105UA/en unknown
- 2013-03-21 US US14/387,861 patent/US20150065371A1/en not_active Abandoned
- 2013-03-21 NZ NZ630091A patent/NZ630091A/en not_active IP Right Cessation
- 2013-03-21 AU AU2013240127A patent/AU2013240127B2/en active Active
- 2013-03-21 JP JP2015503378A patent/JP6225163B2/ja active Active
- 2013-03-21 CN CN201380017702.4A patent/CN104520713A/zh active Pending
- 2013-03-21 EP EP13715086.8A patent/EP2831591B1/en active Active
- 2013-03-21 CA CA2867508A patent/CA2867508C/en active Active
- 2013-03-21 KR KR20147027562A patent/KR20140142715A/ko not_active Withdrawn
- 2013-03-21 IN IN7531DEN2014 patent/IN2014DN07531A/en unknown
- 2013-03-21 RU RU2014137129A patent/RU2014137129A/ru not_active Application Discontinuation
-
2024
- 2024-08-30 US US18/820,303 patent/US20250102507A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015514212A5 (enExample) | ||
| US20250002980A1 (en) | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics | |
| Park et al. | Spatial transcriptomics: technical aspects of recent developments and their applications in neuroscience and cancer research | |
| Lyubimova et al. | Single-molecule mRNA detection and counting in mammalian tissue | |
| EP3490616B1 (en) | Highly-multiplexed fluorescent imaging | |
| US20140270435A1 (en) | Spacial sequencing/gene expression camera | |
| EP2279266B1 (en) | A method for detecting igf1r aberrations in circulating tumor cells using fish | |
| CN110055325A (zh) | 用于前列腺癌的诊断、预后和评估治疗性/预防性治疗的材料和方法 | |
| RU2014137125A (ru) | Способы формирования изображения биологического образца | |
| US10685210B2 (en) | Tissue microarray registration and analysis | |
| Pajor et al. | State‐of‐the‐art FISHing: Automated analysis of cytogenetic aberrations in interphase nuclei | |
| Duan et al. | Spatially resolved transcriptomics: advances and applications | |
| JP2010508028A (ja) | 癌および高度の過形成の自動検出方法 | |
| JP2022520603A (ja) | マルチチャネル画像における自己蛍光の寄与を算出するシステムおよび方法 | |
| Theodosiou et al. | Evaluation of FISH image analysis system on assessing HER2 amplification in breast carcinoma cases | |
| Rygiel et al. | Efficient automated assessment of genetic abnormalities detected by fluorescence in situ hybridization on brush cytology in a Barrett esophagus surveillance population | |
| Ren et al. | Systematic benchmarking of high-throughput subcellular spatial transcriptomics platforms | |
| Gillespie et al. | Molecular profiling of cancer | |
| Chen et al. | Advances in cancer tissue microarray technology: Towards improved understanding and diagnostics | |
| Aung et al. | High-plex assessment of biomarkers in tumors | |
| US9096902B2 (en) | Genetic barcodes | |
| Harris et al. | Quest for the ideal cancer biomarker: an update on progress in capture and characterization of circulating tumor cells | |
| WO2024167925A1 (en) | Convolutional neural network model for imaging via fluorescence in situ hybridization | |
| JP2011511635A (ja) | 癌標識としての結腸癌関連転写因子１（ｃｃａｔ−１） | |
| Ostalecki et al. | Combined multi-gene analysis at the RNA and protein levels in single FFPE tissue sections |