JP2015507931A5 - - Google Patents
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- Publication number
- JP2015507931A5 JP2015507931A5 JP2014557780A JP2014557780A JP2015507931A5 JP 2015507931 A5 JP2015507931 A5 JP 2015507931A5 JP 2014557780 A JP2014557780 A JP 2014557780A JP 2014557780 A JP2014557780 A JP 2014557780A JP 2015507931 A5 JP2015507931 A5 JP 2015507931A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- rnase
- enzyme
- flap
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 32
- 102100034343 Integrase Human genes 0.000 claims description 13
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims description 12
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 10
- 108060004795 Methyltransferase Proteins 0.000 claims description 9
- 108091093088 Amplicon Proteins 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 125000003729 nucleotide group Chemical group 0.000 claims description 8
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 8
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 claims description 6
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 claims description 6
- 229940104302 cytosine Drugs 0.000 claims description 6
- 230000001419 dependent effect Effects 0.000 claims description 6
- 229940029575 guanosine Drugs 0.000 claims description 6
- 238000012986 modification Methods 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 6
- 230000000903 blocking effect Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 108010083644 Ribonucleases Proteins 0.000 claims description 4
- 102000006382 Ribonucleases Human genes 0.000 claims description 4
- 108091028664 Ribonucleotide Proteins 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 239000002336 ribonucleotide Substances 0.000 claims description 4
- 235000011178 triphosphate Nutrition 0.000 claims description 4
- 239000001226 triphosphate Substances 0.000 claims description 4
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- 102100025290 Ribonuclease H1 Human genes 0.000 claims description 2
- 238000003556 assay Methods 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims description 2
- 238000003776 cleavage reaction Methods 0.000 claims description 2
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000002844 melting Methods 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- 150000007523 nucleic acids Chemical group 0.000 claims description 2
- 108010052833 ribonuclease HI Proteins 0.000 claims description 2
- 230000007017 scission Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 claims 4
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 238000002405 diagnostic procedure Methods 0.000 claims 1
- 102000023732 binding proteins Human genes 0.000 description 2
- 108091008324 binding proteins Proteins 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261598648P | 2012-02-14 | 2012-02-14 | |
| US61/598,648 | 2012-02-14 | ||
| PCT/US2013/026202 WO2013123238A1 (en) | 2012-02-14 | 2013-02-14 | Methods of isothermal amplification using blocked primers |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015507931A JP2015507931A (ja) | 2015-03-16 |
| JP2015507931A5 true JP2015507931A5 (enrdf_load_stackoverflow) | 2016-03-31 |
| JP6466173B2 JP6466173B2 (ja) | 2019-02-06 |
Family
ID=48984714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557780A Active JP6466173B2 (ja) | 2012-02-14 | 2013-02-14 | ブロック型プライマーを用いた等温増幅法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8936921B2 (enrdf_load_stackoverflow) |
| EP (1) | EP2814975A4 (enrdf_load_stackoverflow) |
| JP (1) | JP6466173B2 (enrdf_load_stackoverflow) |
| CA (1) | CA2881200A1 (enrdf_load_stackoverflow) |
| HK (1) | HK1205194A1 (enrdf_load_stackoverflow) |
| WO (1) | WO2013123238A1 (enrdf_load_stackoverflow) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| CN104722342B (zh) | 2009-03-24 | 2017-01-11 | 芝加哥大学 | 滑动式芯片装置和方法 |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| AU2013249007B2 (en) | 2012-04-20 | 2016-04-21 | California Institute Of Technology | Fluidic devices and systems for sample preparation or autonomous analysis |
| US9808798B2 (en) | 2012-04-20 | 2017-11-07 | California Institute Of Technology | Fluidic devices for biospecimen preservation |
| US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
| US9074248B1 (en) * | 2012-12-18 | 2015-07-07 | Qiagen Gmbh | Primers for helicase dependent amplification and their methods of use |
| AU2013381709A1 (en) * | 2013-03-15 | 2015-10-01 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US20170218438A1 (en) * | 2016-02-01 | 2017-08-03 | Integrated Dna Technologies, Inc. | Cleavable primers for isothermal amplification |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| EP3469078B1 (en) * | 2016-06-10 | 2024-03-27 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| JP7279633B2 (ja) * | 2017-03-29 | 2023-05-23 | 東洋紡株式会社 | 核酸の検出方法 |
| CN120210336A (zh) | 2017-10-06 | 2025-06-27 | 10X基因组学有限公司 | Rna模板化连接 |
| CN111566118A (zh) | 2017-10-18 | 2020-08-21 | 零日诊断有限公司 | 通过靶向抑制dna扩增来选择性富集混合dna样品中的dna群体 |
| EP3707274B1 (en) | 2017-11-07 | 2023-09-13 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| US12226745B2 (en) | 2017-11-07 | 2025-02-18 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| CN109913538A (zh) * | 2018-09-06 | 2019-06-21 | 湖南融健基因生物科技有限公司 | 一种快速检测snp位点的荧光定量试剂盒 |
| EP3963103B1 (en) * | 2019-05-03 | 2024-02-28 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
| US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| JP3681729B2 (ja) | 2001-02-15 | 2005-08-10 | タカラバイオ株式会社 | 塩基置換の検出方法 |
| TW200404889A (en) | 2002-05-31 | 2004-04-01 | Takara Bio Inc | Method of typing gene polymorphisms |
| JP4632788B2 (ja) * | 2002-09-20 | 2011-02-16 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | 核酸のヘリカーゼ依存性増幅 |
| US7662594B2 (en) * | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| WO2006087574A2 (en) * | 2005-02-19 | 2006-08-24 | Geneform Technologies Limited | Isothermal nucleic acid amplification |
| WO2007120808A2 (en) | 2006-04-14 | 2007-10-25 | Biohelix Corporation | A method of asymmetric helicase-dependent amplification for probe-based detection of targets |
| EP2644707B1 (en) * | 2008-04-30 | 2015-06-03 | Integrated Dna Technologies, Inc. | RNase-H-based assays utilizing modified RNA monomers |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| CA2777446A1 (en) * | 2009-10-13 | 2011-04-21 | Syntezza Molecular Detection Israel Ltd. | Methods and compositions for amplifying target sequences from nucleic acid samples |
| CN102791884A (zh) * | 2010-01-08 | 2012-11-21 | 奇亚根盖瑟斯堡股份有限公司 | 用于等温核酸扩增的材料和方法 |
| WO2011146629A2 (en) * | 2010-05-19 | 2011-11-24 | Qiagen Gaithersburg Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
-
2013
- 2013-02-14 EP EP13748794.8A patent/EP2814975A4/en not_active Withdrawn
- 2013-02-14 HK HK15105687.0A patent/HK1205194A1/xx unknown
- 2013-02-14 WO PCT/US2013/026202 patent/WO2013123238A1/en active Application Filing
- 2013-02-14 JP JP2014557780A patent/JP6466173B2/ja active Active
- 2013-02-14 CA CA2881200A patent/CA2881200A1/en not_active Abandoned
- 2013-06-03 US US13/908,827 patent/US8936921B2/en active Active
-
2014
- 2014-12-10 US US14/565,696 patent/US9896723B2/en active Active
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