JP2015507931A5 - - Google Patents
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- Publication number
- JP2015507931A5 JP2015507931A5 JP2014557780A JP2014557780A JP2015507931A5 JP 2015507931 A5 JP2015507931 A5 JP 2015507931A5 JP 2014557780 A JP2014557780 A JP 2014557780A JP 2014557780 A JP2014557780 A JP 2014557780A JP 2015507931 A5 JP2015507931 A5 JP 2015507931A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- rnase
- enzyme
- flap
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 32
- 102100034343 Integrase Human genes 0.000 claims description 13
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims description 12
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 10
- 108060004795 Methyltransferase Proteins 0.000 claims description 9
- 108091093088 Amplicon Proteins 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 125000003729 nucleotide group Chemical group 0.000 claims description 8
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 8
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 claims description 6
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 claims description 6
- 229940104302 cytosine Drugs 0.000 claims description 6
- 230000001419 dependent effect Effects 0.000 claims description 6
- 229940029575 guanosine Drugs 0.000 claims description 6
- 238000012986 modification Methods 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 6
- 230000000903 blocking effect Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 108010083644 Ribonucleases Proteins 0.000 claims description 4
- 102000006382 Ribonucleases Human genes 0.000 claims description 4
- 108091028664 Ribonucleotide Proteins 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 239000002336 ribonucleotide Substances 0.000 claims description 4
- 235000011178 triphosphate Nutrition 0.000 claims description 4
- 239000001226 triphosphate Substances 0.000 claims description 4
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- 102100025290 Ribonuclease H1 Human genes 0.000 claims description 2
- 238000003556 assay Methods 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims description 2
- 238000003776 cleavage reaction Methods 0.000 claims description 2
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000002844 melting Methods 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- 150000007523 nucleic acids Chemical group 0.000 claims description 2
- 108010052833 ribonuclease HI Proteins 0.000 claims description 2
- 230000007017 scission Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 claims 4
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 238000002405 diagnostic procedure Methods 0.000 claims 1
- 102000023732 binding proteins Human genes 0.000 description 2
- 108091008324 binding proteins Proteins 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261598648P | 2012-02-14 | 2012-02-14 | |
| US61/598,648 | 2012-02-14 | ||
| PCT/US2013/026202 WO2013123238A1 (en) | 2012-02-14 | 2013-02-14 | Methods of isothermal amplification using blocked primers |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015507931A JP2015507931A (ja) | 2015-03-16 |
| JP2015507931A5 true JP2015507931A5 (cg-RX-API-DMAC7.html) | 2016-03-31 |
| JP6466173B2 JP6466173B2 (ja) | 2019-02-06 |
Family
ID=48984714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557780A Active JP6466173B2 (ja) | 2012-02-14 | 2013-02-14 | ブロック型プライマーを用いた等温増幅法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8936921B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2814975A4 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6466173B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2881200A1 (cg-RX-API-DMAC7.html) |
| HK (1) | HK1205194A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2013123238A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| WO2014143228A1 (en) * | 2013-03-15 | 2014-09-18 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| US9415392B2 (en) | 2009-03-24 | 2016-08-16 | The University Of Chicago | Slip chip device and methods |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| JP6196661B2 (ja) | 2012-04-20 | 2017-09-13 | スリップチップ， エルエルシー | サンプル調製または自律分析のための流体デバイスおよびシステム |
| US9808798B2 (en) | 2012-04-20 | 2017-11-07 | California Institute Of Technology | Fluidic devices for biospecimen preservation |
| US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
| US9074248B1 (en) * | 2012-12-18 | 2015-07-07 | Qiagen Gmbh | Primers for helicase dependent amplification and their methods of use |
| WO2017136387A1 (en) * | 2016-02-01 | 2017-08-10 | Integrated Dna Technologies, Inc. | Cleavable primers for isothermal amplification |
| US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| WO2017214561A1 (en) * | 2016-06-10 | 2017-12-14 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
| WO2018180987A1 (ja) * | 2017-03-29 | 2018-10-04 | 東洋紡株式会社 | 核酸の検出方法 |
| ES3029737T3 (en) | 2017-10-06 | 2025-06-25 | 10X Genomics Inc | Rna templated ligation |
| CN111566118A (zh) * | 2017-10-18 | 2020-08-21 | 零日诊断有限公司 | 通过靶向抑制dna扩增来选择性富集混合dna样品中的dna群体 |
| US12226745B2 (en) | 2017-11-07 | 2025-02-18 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| CN111566223B (zh) | 2017-11-07 | 2024-04-12 | 生命技术公司 | 用于操纵核酸的方法和组合物 |
| CN109913538A (zh) * | 2018-09-06 | 2019-06-21 | 湖南融健基因生物科技有限公司 | 一种快速检测snp位点的荧光定量试剂盒 |
| EP3963103B1 (en) * | 2019-05-03 | 2024-02-28 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
| US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
| US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| JP3681729B2 (ja) | 2001-02-15 | 2005-08-10 | タカラバイオ株式会社 | 塩基置換の検出方法 |
| TW200404889A (en) | 2002-05-31 | 2004-04-01 | Takara Bio Inc | Method of typing gene polymorphisms |
| DE60324810D1 (de) | 2002-09-20 | 2009-01-02 | New England Biolabs Inc | HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN |
| US7662594B2 (en) * | 2002-09-20 | 2010-02-16 | New England Biolabs, Inc. | Helicase-dependent amplification of RNA |
| US7824890B2 (en) * | 2005-02-19 | 2010-11-02 | Avacta Group Plc | Isothermal amplification of nucleic acids |
| WO2007120808A2 (en) * | 2006-04-14 | 2007-10-25 | Biohelix Corporation | A method of asymmetric helicase-dependent amplification for probe-based detection of targets |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| DK2644707T3 (en) | 2008-04-30 | 2015-06-29 | Integrated Dna Tech Inc | RNase-H-based assays using modified RNA monomers. |
| CA2777446A1 (en) * | 2009-10-13 | 2011-04-21 | Syntezza Molecular Detection Israel Ltd. | Methods and compositions for amplifying target sequences from nucleic acid samples |
| CA2786473A1 (en) * | 2010-01-08 | 2011-07-14 | Qiagen Gaithersburg, Inc. | Materials and methods for isothermal nucleic acid amplification |
| JP2013528049A (ja) * | 2010-05-19 | 2013-07-08 | キアゲン ガイサーズバーグ アイエヌシー． | 核酸の配列特異的な精製及び多重分析のための方法及び組成物 |
-
2013
- 2013-02-14 HK HK15105687.0A patent/HK1205194A1/xx unknown
- 2013-02-14 CA CA2881200A patent/CA2881200A1/en not_active Abandoned
- 2013-02-14 EP EP13748794.8A patent/EP2814975A4/en not_active Withdrawn
- 2013-02-14 WO PCT/US2013/026202 patent/WO2013123238A1/en not_active Ceased
- 2013-02-14 JP JP2014557780A patent/JP6466173B2/ja active Active
- 2013-06-03 US US13/908,827 patent/US8936921B2/en active Active
-
2014
- 2014-12-10 US US14/565,696 patent/US9896723B2/en active Active
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