JP2014523405A5 - - Google Patents

Download PDF

Info

Publication number
JP2014523405A5
JP2014523405A5 JP2014508565A JP2014508565A JP2014523405A5 JP 2014523405 A5 JP2014523405 A5 JP 2014523405A5 JP 2014508565 A JP2014508565 A JP 2014508565A JP 2014508565 A JP2014508565 A JP 2014508565A JP 2014523405 A5 JP2014523405 A5 JP 2014523405A5
Authority
JP
Japan
Prior art keywords
ligand
sample
target molecule
cation exchange
cell culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2014508565A
Other languages
English (en)
Japanese (ja)
Other versions
JP6068442B2 (ja
JP2014523405A (ja
Filing date
Publication date
Priority claimed from US13/096,699 external-priority patent/US8911992B2/en
Application filed filed Critical
Publication of JP2014523405A publication Critical patent/JP2014523405A/ja
Publication of JP2014523405A5 publication Critical patent/JP2014523405A5/ja
Application granted granted Critical
Publication of JP6068442B2 publication Critical patent/JP6068442B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2014508565A 2011-04-28 2012-04-26 標的分子精製におけるｄｎａ除去法 Expired - Fee Related JP6068442B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/096,699 2011-04-28
US13/096,699 US8911992B2 (en) 2011-04-28 2011-04-28 DNA removal in target molecule purification
PCT/US2012/035272 WO2012149200A1 (en) 2011-04-28 2012-04-26 Dna removal in target molecule purification

Publications (3)

Publication Number Publication Date
JP2014523405A JP2014523405A (ja) 2014-09-11
JP2014523405A5 true JP2014523405A5 (https=) 2015-05-28
JP6068442B2 JP6068442B2 (ja) 2017-01-25

Family

ID=47068181

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014508565A Expired - Fee Related JP6068442B2 (ja) 2011-04-28 2012-04-26 標的分子精製におけるｄｎａ除去法

Country Status (5)

Country Link
US (1) US8911992B2 (https=)
EP (1) EP2702065B1 (https=)
JP (1) JP6068442B2 (https=)
CA (1) CA2832341C (https=)
WO (1) WO2012149200A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2854982B1 (en) 2012-05-31 2017-11-01 Agency For Science, Technology And Research Chromatographic purification of polynucleotides with negatively charged particles
WO2016153983A1 (en) * 2015-03-20 2016-09-29 Bristol-Myers Squibb Company Use of dextran for protein purification
CN119874910A (zh) * 2019-06-07 2025-04-25 达因疗法公司 制备蛋白质-寡核苷酸复合物的方法
JP2023539163A (ja) * 2020-08-18 2023-09-13 ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー． 宿主細胞ｄｎａの金属アフィニティー抽出
JP2024544537A (ja) * 2021-11-11 2024-12-03 プロメガ コーポレイション 精子関連ｄｎａ精製のための陽イオン交換

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6504021B2 (en) 2000-07-05 2003-01-07 Edge Biosystems, Inc. Ion exchange method for DNA purification
US20100056762A1 (en) * 2001-05-11 2010-03-04 Old Lloyd J Specific binding proteins and uses thereof
WO2003059935A2 (en) * 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
KR20140071452A (ko) 2006-04-05 2014-06-11 애브비 바이오테크놀로지 리미티드 항체 정제
BRPI0812288A2 (pt) * 2007-06-01 2014-11-25 Hoffmann La Roche Purificação de imunoglobulina.
EP2275431A3 (en) * 2008-01-18 2011-05-25 Bio-Rad Laboratories, Inc. Enhanced fractionation of phosphorylated and nonphosphorylated biomolecules by apatite chromotography
MX2010014180A (es) * 2008-06-24 2011-02-15 Reddys Lab Inc Dr Purificacion de citocinas modificadas.
JP5463492B2 (ja) * 2008-10-08 2014-04-09 島根県 微生物細胞からのプラスミドｄｎａ抽出法

Similar Documents

Publication Publication Date Title
JP6410734B2 (ja) タンパク質調製物の凝集体含有量を低減する方法
Pedersen et al. Specific and generic isolation of extracellular vesicles with magnetic beads
JP6385374B2 (ja) タンパク質製剤からエンドトキシンを除去するための物質および方法
US20150275269A1 (en) Method for purifying nucleic acid and kit
JP2014532718A5 (https=)
Dhadge et al. Magnetic aqueous two phase fishing: a hybrid process technology for antibody purification
JP6268167B2 (ja) タンパク質製剤中の凝集物含量を低減するために混合型多官能表面を使用する方法
JP2011510303A (ja) アパタイトクロマトグラフィーによるリン酸化生体分子および非リン酸化生体分子の向上した精製法
JP2016509069A (ja) 非イオン性有機ポリマー存在下、高導電率でのタンパク質精製
JP2014523405A5 (https=)
JP2016507588A (ja) 非イオン性有機ポリマーおよび電気陽性表面の存在下でのタンパク質精製
JP6320429B2 (ja) 負帯電粒子を用いるウイルス調製物のクロマトグラフィー精製
CN104136121B (zh) 减少离子交换色谱中的pH漂移
JP6068442B2 (ja) 標的分子精製におけるｄｎａ除去法
JP2016529908A5 (https=)
Rosti et al. Recovery of microquantities of human epidermal growth factor from Escherichia coli Homogenate and Pichia pastoris culture medium using expanded bed adsorption
WO2013121216A1 (en) High efficiency cell capture
JP6403790B2 (ja) タンパク質調製物の凝集体含有量をアリールアニオン処理によって減少させる方法
WO2015099550A1 (en) Magnetic liquid-liquid extraction for purification and partitioning of substances
JP2016513245A (ja) タンパク質製剤の凝集体含有量を減少させる混合多官能金属親和性表面
JP2017524921A5 (https=)
Liangsupree et al. Immunoaffinity Chromatography
Fischer et al. Nanoparticle mediated protein separation in aqueous micellar two-phase systems
Hanora Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels
JP2024093998A (ja) 非特異反応を抑制可能な抗体溶液