JP2014502496A5 - - Google Patents
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- Publication number
- JP2014502496A5 JP2014502496A5 JP2013544844A JP2013544844A JP2014502496A5 JP 2014502496 A5 JP2014502496 A5 JP 2014502496A5 JP 2013544844 A JP2013544844 A JP 2013544844A JP 2013544844 A JP2013544844 A JP 2013544844A JP 2014502496 A5 JP2014502496 A5 JP 2014502496A5
- Authority
- JP
- Japan
- Prior art keywords
- dye
- passive reference
- carboxy
- rhodamine
- reference dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000975 dye Substances 0.000 claims description 158
- 239000001022 rhodamine dye Substances 0.000 claims description 83
- WQZIDRAQTRIQDX-UHFFFAOYSA-N 6-carboxy-x-rhodamine Chemical compound OC(=O)C1=CC=C(C([O-])=O)C=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 WQZIDRAQTRIQDX-UHFFFAOYSA-N 0.000 claims description 49
- UNGMOMJDNDFGJG-UHFFFAOYSA-N 5-carboxy-X-rhodamine Chemical compound [O-]C(=O)C1=CC(C(=O)O)=CC=C1C1=C(C=C2C3=C4CCCN3CCC2)C4=[O+]C2=C1C=C1CCCN3CCCC2=C13 UNGMOMJDNDFGJG-UHFFFAOYSA-N 0.000 claims description 47
- 239000000203 mixture Substances 0.000 claims description 42
- 238000000034 method Methods 0.000 claims description 38
- 238000010606 normalization Methods 0.000 claims description 25
- 230000005284 excitation Effects 0.000 claims description 23
- 238000003752 polymerase chain reaction Methods 0.000 claims description 16
- 230000003321 amplification Effects 0.000 claims description 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 13
- 238000003753 real-time PCR Methods 0.000 claims description 10
- 239000001226 triphosphate Substances 0.000 claims description 10
- 235000011178 triphosphate Nutrition 0.000 claims description 10
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims description 10
- 108020004707 nucleic acids Proteins 0.000 claims description 8
- 150000007523 nucleic acids Chemical class 0.000 claims description 8
- 102000039446 nucleic acids Human genes 0.000 claims description 8
- 239000011541 reaction mixture Substances 0.000 claims description 8
- 102100034343 Integrase Human genes 0.000 claims description 7
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- 238000009830 intercalation Methods 0.000 claims description 6
- 239000003155 DNA primer Substances 0.000 claims description 4
- 239000007850 fluorescent dye Substances 0.000 claims description 3
- 239000013615 primer Substances 0.000 claims description 3
- 108091034117 Oligonucleotide Proteins 0.000 claims description 2
- 239000012472 biological sample Substances 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US42393210P | 2010-12-16 | 2010-12-16 | |
| US61/423,932 | 2010-12-16 | ||
| US201161435209P | 2011-01-21 | 2011-01-21 | |
| US61/435,209 | 2011-01-21 | ||
| US201161508453P | 2011-07-15 | 2011-07-15 | |
| US61/508,453 | 2011-07-15 | ||
| PCT/US2011/065617 WO2012083235A1 (en) | 2010-12-16 | 2011-12-16 | Universal reference dye for quantitative amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014502496A JP2014502496A (ja) | 2014-02-03 |
| JP2014502496A5 true JP2014502496A5 (https=) | 2015-01-22 |
| JP5985503B2 JP5985503B2 (ja) | 2016-09-06 |
Family
ID=46245139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013544844A Active JP5985503B2 (ja) | 2010-12-16 | 2011-12-16 | 定量的増幅のためのユニバーサルリファレンス色素 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9493824B2 (https=) |
| EP (1) | EP2652154B1 (https=) |
| JP (1) | JP5985503B2 (https=) |
| CA (1) | CA2820715C (https=) |
| WO (1) | WO2012083235A1 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| EP2951320B1 (en) | 2013-02-01 | 2018-12-26 | Bio-Rad Laboratories, Inc. | Multiplexed digital assay with specific and generic reporters |
| EP3543207B1 (en) * | 2013-03-05 | 2025-04-23 | University of Washington through its Center for Commercialization | Western blot analysis using polymer dots |
| US20140356968A1 (en) * | 2013-06-04 | 2014-12-04 | Therapeutic Proteins International, LLC | Methods for comparing a structure of a first biomolecule and a second biomolecule |
| US9353311B2 (en) | 2013-10-10 | 2016-05-31 | Bio-Rad Laboratories, Inc. | Composite visible colorant and method for quantitative amplification |
| US20170002398A1 (en) * | 2014-01-23 | 2017-01-05 | Qiagen Gmbh | Nucleic acid amplification method |
| CN108351918B (zh) | 2015-11-20 | 2021-09-17 | Seegene株式会社 | 对目标分析物的数据集的校准方法 |
| US10526536B2 (en) | 2015-12-22 | 2020-01-07 | Roche Molecular Systems, Inc. | Compositions with thermolabile dyes |
| US11072820B2 (en) | 2017-10-19 | 2021-07-27 | Bio-Rad Laboratories, Inc. | Digital amplification assays with unconventional and/or inverse changes in photoluminescence |
| JP7532820B2 (ja) * | 2019-03-20 | 2024-08-14 | 東洋紡株式会社 | 非特異増幅が抑制された核酸増幅法 |
| EP4419711A1 (en) * | 2021-10-20 | 2024-08-28 | Qiagen GmbH | High stokes shift fluorescence dyes for multiplex detection |
| CN119462681B (zh) * | 2024-10-16 | 2025-09-30 | 聊城柏莱斯生物科技有限公司 | 一种荧光定量pcr参比染料及其制备方法与应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5654419A (en) * | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| US6028190A (en) * | 1994-02-01 | 2000-02-22 | The Regents Of The University Of California | Probes labeled with energy transfer coupled dyes |
| US5928927A (en) | 1996-04-16 | 1999-07-27 | The United States Of America As Represented By The Secretary Of The Army | Enzymatic detoxification of organophosphorus compounds |
| US5736333A (en) * | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
| US7498164B2 (en) * | 1998-05-16 | 2009-03-03 | Applied Biosystems, Llc | Instrument for monitoring nucleic acid sequence amplification reaction |
| US7410793B2 (en) * | 1999-05-17 | 2008-08-12 | Applera Corporation | Optical instrument including excitation source |
| US7166478B2 (en) | 2002-03-12 | 2007-01-23 | Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. | Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses |
| US20060002474A1 (en) * | 2004-06-26 | 2006-01-05 | Oscar Chi-Lim Au | Efficient multi-block motion estimation for video compression |
| JP2006101766A (ja) * | 2004-10-05 | 2006-04-20 | Plant Genome Center Co Ltd | 遺伝子の発現レベルを指標とした穀物の食味判定方法 |
| US7776567B2 (en) | 2005-03-17 | 2010-08-17 | Biotium, Inc. | Dimeric and trimeric nucleic acid dyes, and associated systems and methods |
| US7919242B2 (en) | 2005-06-30 | 2011-04-05 | Roche Molecular Systems, Inc. | Light emission modifiers and their uses in nucleic acid detection, amplification and analysis |
| US7785786B2 (en) | 2006-01-23 | 2010-08-31 | Quest Diagnostics Investments Incorporated | Methods for detecting nucleic acids using multiple signals |
| US20080001099A1 (en) | 2006-07-01 | 2008-01-03 | Sharaf Muhammad A | Quantitative calibration method and system for genetic analysis instrumentation |
| CN102851369B (zh) | 2006-12-13 | 2015-01-21 | 卢米耐克斯公司 | 用于实时pcr多重分析的系统和方法 |
| US7872116B2 (en) * | 2007-02-06 | 2011-01-18 | American Type Culture Collection (Atcc) | Identification of cell culture contaminants among Mollicutes species by a PCR based assay |
| CN101896623B (zh) | 2007-10-22 | 2013-12-18 | Lgc有限公司 | 寡核苷酸及其应用 |
| WO2009117327A2 (en) | 2008-03-15 | 2009-09-24 | Hologic, Inc. | Compositions and methods for analysis of nucleic acid molecules during amplification reactions |
| EP2192198A1 (en) | 2008-12-01 | 2010-06-02 | Biotype AG | Novel combination of fluorescent dyes for the detection of nucleic acids |
| JP5746135B2 (ja) | 2009-03-16 | 2015-07-08 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 核酸結合色素およびその使用 |
| CN103703141B (zh) * | 2011-04-08 | 2016-08-17 | 伯乐实验室公司 | 具有降低的非特异性活性的pcr反应混合物 |
-
2011
- 2011-12-16 JP JP2013544844A patent/JP5985503B2/ja active Active
- 2011-12-16 US US13/328,154 patent/US9493824B2/en active Active
- 2011-12-16 WO PCT/US2011/065617 patent/WO2012083235A1/en not_active Ceased
- 2011-12-16 EP EP11848622.4A patent/EP2652154B1/en active Active
- 2011-12-16 CA CA2820715A patent/CA2820715C/en active Active
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