CA2820715C - Universal reference dye for quantitative amplification - Google Patents

Universal reference dye for quantitative amplification Download PDF

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Publication number
CA2820715C
CA2820715C CA2820715A CA2820715A CA2820715C CA 2820715 C CA2820715 C CA 2820715C CA 2820715 A CA2820715 A CA 2820715A CA 2820715 A CA2820715 A CA 2820715A CA 2820715 C CA2820715 C CA 2820715C
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CA
Canada
Prior art keywords
dye
passive reference
reference dye
passive
mixture
Prior art date
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Active
Application number
CA2820715A
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English (en)
French (fr)
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CA2820715A1 (en
Inventor
Yan Wang
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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Publication date
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Publication of CA2820715A1 publication Critical patent/CA2820715A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2820715A 2010-12-16 2011-12-16 Universal reference dye for quantitative amplification Active CA2820715C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US42393210P 2010-12-16 2010-12-16
US61/423,932 2010-12-16
US201161435209P 2011-01-21 2011-01-21
US61/435,209 2011-01-21
US201161508453P 2011-07-15 2011-07-15
US61/508,453 2011-07-15
PCT/US2011/065617 WO2012083235A1 (en) 2010-12-16 2011-12-16 Universal reference dye for quantitative amplification

Publications (2)

Publication Number Publication Date
CA2820715A1 CA2820715A1 (en) 2012-06-21
CA2820715C true CA2820715C (en) 2020-03-31

Family

ID=46245139

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2820715A Active CA2820715C (en) 2010-12-16 2011-12-16 Universal reference dye for quantitative amplification

Country Status (5)

Country Link
US (1) US9493824B2 (https=)
EP (1) EP2652154B1 (https=)
JP (1) JP5985503B2 (https=)
CA (1) CA2820715C (https=)
WO (1) WO2012083235A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US9970052B2 (en) 2012-08-23 2018-05-15 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
EP2951320B1 (en) 2013-02-01 2018-12-26 Bio-Rad Laboratories, Inc. Multiplexed digital assay with specific and generic reporters
EP3543207B1 (en) * 2013-03-05 2025-04-23 University of Washington through its Center for Commercialization Western blot analysis using polymer dots
US20140356968A1 (en) * 2013-06-04 2014-12-04 Therapeutic Proteins International, LLC Methods for comparing a structure of a first biomolecule and a second biomolecule
US9353311B2 (en) 2013-10-10 2016-05-31 Bio-Rad Laboratories, Inc. Composite visible colorant and method for quantitative amplification
US20170002398A1 (en) * 2014-01-23 2017-01-05 Qiagen Gmbh Nucleic acid amplification method
CN108351918B (zh) 2015-11-20 2021-09-17 Seegene株式会社 对目标分析物的数据集的校准方法
US10526536B2 (en) 2015-12-22 2020-01-07 Roche Molecular Systems, Inc. Compositions with thermolabile dyes
US11072820B2 (en) 2017-10-19 2021-07-27 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
JP7532820B2 (ja) * 2019-03-20 2024-08-14 東洋紡株式会社 非特異増幅が抑制された核酸増幅法
EP4419711A1 (en) * 2021-10-20 2024-08-28 Qiagen GmbH High stokes shift fluorescence dyes for multiplex detection
CN119462681B (zh) * 2024-10-16 2025-09-30 聊城柏莱斯生物科技有限公司 一种荧光定量pcr参比染料及其制备方法与应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US6028190A (en) * 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5928927A (en) 1996-04-16 1999-07-27 The United States Of America As Represented By The Secretary Of The Army Enzymatic detoxification of organophosphorus compounds
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US7498164B2 (en) * 1998-05-16 2009-03-03 Applied Biosystems, Llc Instrument for monitoring nucleic acid sequence amplification reaction
US7410793B2 (en) * 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
US7166478B2 (en) 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US20060002474A1 (en) * 2004-06-26 2006-01-05 Oscar Chi-Lim Au Efficient multi-block motion estimation for video compression
JP2006101766A (ja) * 2004-10-05 2006-04-20 Plant Genome Center Co Ltd 遺伝子の発現レベルを指標とした穀物の食味判定方法
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7919242B2 (en) 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
US20080001099A1 (en) 2006-07-01 2008-01-03 Sharaf Muhammad A Quantitative calibration method and system for genetic analysis instrumentation
CN102851369B (zh) 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
US7872116B2 (en) * 2007-02-06 2011-01-18 American Type Culture Collection (Atcc) Identification of cell culture contaminants among Mollicutes species by a PCR based assay
CN101896623B (zh) 2007-10-22 2013-12-18 Lgc有限公司 寡核苷酸及其应用
WO2009117327A2 (en) 2008-03-15 2009-09-24 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
EP2192198A1 (en) 2008-12-01 2010-06-02 Biotype AG Novel combination of fluorescent dyes for the detection of nucleic acids
JP5746135B2 (ja) 2009-03-16 2015-07-08 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 核酸結合色素およびその使用
CN103703141B (zh) * 2011-04-08 2016-08-17 伯乐实验室公司 具有降低的非特异性活性的pcr反应混合物

Also Published As

Publication number Publication date
JP5985503B2 (ja) 2016-09-06
US20120164690A1 (en) 2012-06-28
US9493824B2 (en) 2016-11-15
CA2820715A1 (en) 2012-06-21
EP2652154B1 (en) 2017-10-18
EP2652154A1 (en) 2013-10-23
WO2012083235A1 (en) 2012-06-21
JP2014502496A (ja) 2014-02-03
EP2652154A4 (en) 2014-12-31

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