JP2014163749A5 - - Google Patents
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- Publication number
- JP2014163749A5 JP2014163749A5 JP2013033567A JP2013033567A JP2014163749A5 JP 2014163749 A5 JP2014163749 A5 JP 2014163749A5 JP 2013033567 A JP2013033567 A JP 2013033567A JP 2013033567 A JP2013033567 A JP 2013033567A JP 2014163749 A5 JP2014163749 A5 JP 2014163749A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- stratum corneum
- manufactured
- added
- sirna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000000434 stratum corneum Anatomy 0.000 description 6
- 108010032428 Protein Deglycase DJ-1 Proteins 0.000 description 4
- 108020004459 Small interfering RNA Proteins 0.000 description 4
- 102000007659 Protein Deglycase DJ-1 Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000012096 transfection reagent Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 238000009020 BCA Protein Assay Kit Methods 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 101150071263 PARK7 gene Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000008004 cell lysis buffer Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013033567A JP5775540B2 (ja) | 2013-02-22 | 2013-02-22 | 皮膚の紫外線照射により負荷される紫外線ストレスの評価方法 |
| TW103104529A TWI600904B (zh) | 2013-02-22 | 2014-02-12 | 皮膚應激蓄積度的評價方法 |
| CN201410054223.6A CN104007057A (zh) | 2013-02-22 | 2014-02-18 | 皮肤应激蓄积量的评价方法 |
| HK14112554.7A HK1199098A1 (en) | 2013-02-22 | 2014-12-15 | Method of evaluating degree of skin stress accumulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013033567A JP5775540B2 (ja) | 2013-02-22 | 2013-02-22 | 皮膚の紫外線照射により負荷される紫外線ストレスの評価方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014163749A JP2014163749A (ja) | 2014-09-08 |
| JP2014163749A5 true JP2014163749A5 (enExample) | 2014-10-16 |
| JP5775540B2 JP5775540B2 (ja) | 2015-09-09 |
Family
ID=51367814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013033567A Active JP5775540B2 (ja) | 2013-02-22 | 2013-02-22 | 皮膚の紫外線照射により負荷される紫外線ストレスの評価方法 |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JP5775540B2 (enExample) |
| CN (1) | CN104007057A (enExample) |
| HK (1) | HK1199098A1 (enExample) |
| TW (1) | TWI600904B (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6419654B2 (ja) * | 2015-06-22 | 2018-11-07 | 株式会社ファンケル | 発毛の状態及び頭皮の圧縮応力の評価方法 |
| JP7256069B2 (ja) * | 2019-05-10 | 2023-04-11 | 株式会社ファンケル | 生体の酸化ストレスの評価方法 |
| CN117990914A (zh) * | 2022-10-28 | 2024-05-07 | 生物岛实验室 | 肌少症的诊断标志物及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005075513A1 (ja) * | 2004-02-04 | 2005-08-18 | National Institute Of Advanced Industrial Science And Technology | システイン残基が酸化されたdj-1誘導体 |
| AU2006246957A1 (en) * | 2005-05-20 | 2006-11-23 | Premd, Inc. | Direct assay of skin protein in skin samples removed by tape stripping |
| CN103278641A (zh) * | 2005-10-21 | 2013-09-04 | 株式会社芳珂 | 特应性皮炎标记物及其利用技术 |
| US20090208985A1 (en) * | 2006-03-17 | 2009-08-20 | Chika Katagiri | Method Of Evaluating Degree Of Skin Sensitivity Using Squamous Cell Carcinoma Antigen As An Indicator Thereof |
| WO2009123373A1 (en) * | 2008-04-04 | 2009-10-08 | Goodgene Inc. | New skin sampling kit which stores nucleic acids in stable status, genetic test methods by using the kit and their practical application |
| FR2938762B1 (fr) * | 2008-11-21 | 2011-02-04 | Oreal | Utilisation cosmetique de proteines de type dj-1 pour le traitement de la secheresse cutanee |
| JP5733839B2 (ja) * | 2010-03-26 | 2015-06-10 | 国立大学法人北海道大学 | 神経変性疾患治療薬 |
| CN101955908B (zh) * | 2010-07-22 | 2013-01-23 | 中国医学科学院皮肤病研究所 | 用于检测物质致敏性和药物筛选的人工表皮体外模型 |
| JP5658696B2 (ja) * | 2011-02-25 | 2015-01-28 | 株式会社ファンケル | 皮膚のストレス蓄積度の評価方法 |
| JP6419654B2 (ja) * | 2015-06-22 | 2018-11-07 | 株式会社ファンケル | 発毛の状態及び頭皮の圧縮応力の評価方法 |
-
2013
- 2013-02-22 JP JP2013033567A patent/JP5775540B2/ja active Active
-
2014
- 2014-02-12 TW TW103104529A patent/TWI600904B/zh not_active IP Right Cessation
- 2014-02-18 CN CN201410054223.6A patent/CN104007057A/zh active Pending
- 2014-12-15 HK HK14112554.7A patent/HK1199098A1/xx unknown
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