JP2014163749A5 - - Google Patents
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- Publication number
- JP2014163749A5 JP2014163749A5 JP2013033567A JP2013033567A JP2014163749A5 JP 2014163749 A5 JP2014163749 A5 JP 2014163749A5 JP 2013033567 A JP2013033567 A JP 2013033567A JP 2013033567 A JP2013033567 A JP 2013033567A JP 2014163749 A5 JP2014163749 A5 JP 2014163749A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- stratum corneum
- manufactured
- added
- sirna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000000434 stratum corneum Anatomy 0.000 description 6
- 108010032428 Protein Deglycase DJ-1 Proteins 0.000 description 4
- 108020004459 Small interfering RNA Proteins 0.000 description 4
- 102000007659 Protein Deglycase DJ-1 Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000012096 transfection reagent Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 238000009020 BCA Protein Assay Kit Methods 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 101150071263 PARK7 gene Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000008004 cell lysis buffer Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013033567A JP5775540B2 (ja) | 2013-02-22 | 2013-02-22 | 皮膚の紫外線照射により負荷される紫外線ストレスの評価方法 |
| TW103104529A TWI600904B (zh) | 2013-02-22 | 2014-02-12 | 皮膚應激蓄積度的評價方法 |
| CN201410054223.6A CN104007057A (zh) | 2013-02-22 | 2014-02-18 | 皮肤应激蓄积量的评价方法 |
| HK14112554.7A HK1199098A1 (en) | 2013-02-22 | 2014-12-15 | Method of evaluating degree of skin stress accumulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013033567A JP5775540B2 (ja) | 2013-02-22 | 2013-02-22 | 皮膚の紫外線照射により負荷される紫外線ストレスの評価方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014163749A JP2014163749A (ja) | 2014-09-08 |
| JP2014163749A5 true JP2014163749A5 (enrdf_load_stackoverflow) | 2014-10-16 |
| JP5775540B2 JP5775540B2 (ja) | 2015-09-09 |
Family
ID=51367814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013033567A Active JP5775540B2 (ja) | 2013-02-22 | 2013-02-22 | 皮膚の紫外線照射により負荷される紫外線ストレスの評価方法 |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JP5775540B2 (enrdf_load_stackoverflow) |
| CN (1) | CN104007057A (enrdf_load_stackoverflow) |
| HK (1) | HK1199098A1 (enrdf_load_stackoverflow) |
| TW (1) | TWI600904B (enrdf_load_stackoverflow) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6419654B2 (ja) * | 2015-06-22 | 2018-11-07 | 株式会社ファンケル | 発毛の状態及び頭皮の圧縮応力の評価方法 |
| JP7256069B2 (ja) * | 2019-05-10 | 2023-04-11 | 株式会社ファンケル | 生体の酸化ストレスの評価方法 |
| CN117990914A (zh) * | 2022-10-28 | 2024-05-07 | 生物岛实验室 | 肌少症的诊断标志物及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2005075513A1 (ja) * | 2004-02-04 | 2008-01-10 | 独立行政法人産業技術総合研究所 | システイン残基が酸化されたｄｊ−１誘導体 |
| JP2008541100A (ja) * | 2005-05-20 | 2008-11-20 | プレエムディ インク． | テープ引き剥がしにより取り除いた皮膚試料の皮膚プロテインの直接分析 |
| KR20080063326A (ko) * | 2005-10-21 | 2008-07-03 | 가부시키가이샤환케루 | 아토피성 피부염 마커와 그의 이용기술 |
| WO2007108523A1 (ja) * | 2006-03-17 | 2007-09-27 | Shiseido Company, Ltd. | 扁平上皮細胞癌関連抗原を指標とする肌の感受性の程度の評価方法 |
| JP2011516061A (ja) * | 2008-04-04 | 2011-05-26 | グッドジーン インク． | 核酸を安定的に保管する新規皮膚遺伝子カードとこれを用いた遺伝子分析方法、並びにこの応用方法 |
| FR2938762B1 (fr) * | 2008-11-21 | 2011-02-04 | Oreal | Utilisation cosmetique de proteines de type dj-1 pour le traitement de la secheresse cutanee |
| EP2554163A4 (en) * | 2010-03-26 | 2013-07-17 | Univ Hokkaido Nat Univ Corp | THERAPEUTIC ACTIVE FOR NEURODEGENERATIVE DISEASES |
| CN101955908B (zh) * | 2010-07-22 | 2013-01-23 | 中国医学科学院皮肤病研究所 | 用于检测物质致敏性和药物筛选的人工表皮体外模型 |
| JP5658696B2 (ja) * | 2011-02-25 | 2015-01-28 | 株式会社ファンケル | 皮膚のストレス蓄積度の評価方法 |
| JP6419654B2 (ja) * | 2015-06-22 | 2018-11-07 | 株式会社ファンケル | 発毛の状態及び頭皮の圧縮応力の評価方法 |
-
2013
- 2013-02-22 JP JP2013033567A patent/JP5775540B2/ja active Active
-
2014
- 2014-02-12 TW TW103104529A patent/TWI600904B/zh not_active IP Right Cessation
- 2014-02-18 CN CN201410054223.6A patent/CN104007057A/zh active Pending
- 2014-12-15 HK HK14112554.7A patent/HK1199098A1/xx unknown
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