JP2013534313A - 腸の炎症状態の生物学的マーカーとしてのｈｍｇｂ１の使用、糞便サンプル中のｈｍｇｂ１を検出するための非侵襲法およびそのキット - Google Patents
腸の炎症状態の生物学的マーカーとしてのｈｍｇｂ１の使用、糞便サンプル中のｈｍｇｂ１を検出するための非侵襲法およびそのキット Download PDFInfo
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| ITRM2010A000442 | 2010-08-05 | ||
| ITRM2010A000442A IT1406051B1 (it) | 2010-08-05 | 2010-08-05 | Uso di hmgb1 come marcatore biologico di infiammazione intestinale umana, metodo non invasivo per la sua rilevazione in campioni fecali e kit relativo. |
| PCT/IT2011/000276 WO2012017466A1 (en) | 2010-08-05 | 2011-08-01 | Use of hmgb1 as' a biological marker of bowel inflammatory conditions, non-invasive method for its detection in fecal samples and kit thereof |
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| JP2013534313A true JP2013534313A (ja) | 2013-09-02 |
| JP2013534313A5 JP2013534313A5 (enExample) | 2014-09-18 |
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| JP (1) | JP2013534313A (enExample) |
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| WO2015067913A1 (en) | 2013-11-07 | 2015-05-14 | Diagnodus Limited | Biomarkers |
| KR102236829B1 (ko) | 2013-03-15 | 2021-04-07 | 프로타고니스트 테라퓨틱스, 인코포레이티드 | 헵시딘 유사체 및 이의 용도 |
| AU2015258863B2 (en) | 2014-05-16 | 2019-10-24 | Protagonist Therapeutics, Inc. | Alpha4beta7 integrin thioether peptide antagonists |
| AU2015289642B2 (en) | 2014-07-17 | 2021-02-25 | Protagonist Therapeutics, Inc. | Oral peptide inhibitors of interleukin-23 receptor and their use to treat inflammatory bowel diseases |
| WO2017117411A1 (en) | 2015-12-30 | 2017-07-06 | Protagonist Therapeutics, Inc. | Analogues of hepcidin mimetics with improved in vivo half lives |
| WO2018089693A2 (en) * | 2016-11-09 | 2018-05-17 | Protagonist Therapeutics, Inc. | Methods for determining and monitoring gastrointestinal inflammation |
| BR112019023772A2 (pt) | 2017-05-12 | 2020-06-09 | Evonik Operations Gmbh | método para detectar doenças induzidas por c. perfringens em animais e kit de diagnóstico |
| IT201700083055A1 (it) | 2017-07-20 | 2019-01-20 | Laura Stronati | “uso della proteina inibitore 2 della dissociazione di rho gdp come marcatore diagnostico e prognostico delle malattie infiammatorie intestinali” |
| IT201700083044A1 (it) | 2017-07-20 | 2019-01-20 | Laura Stronati | “uso della gelsolina come marcatore diagnostico e prognostico delle malattie infiammatorie intestinali” |
| EP3749345A4 (en) | 2018-02-08 | 2022-04-06 | Protagonist Therapeutics, Inc. | CONJUGATED HEPCIDIN MIMETICS |
| US20210011027A1 (en) * | 2018-03-02 | 2021-01-14 | Evonik Operations Gmbh | In vitro method for detecting intestinal barrier failure in animals by determining ovotransferrin |
| CN109030817A (zh) * | 2018-07-04 | 2018-12-18 | 长沙都正医学检验有限责任公司 | Hmgb1检测试剂盒及其制备方法 |
| TW202019948A (zh) | 2018-07-12 | 2020-06-01 | 美商領導醫療有限公司 | 介白素-23受體之肽抑制劑及其治療發炎疾病之用途 |
| MX2021006917A (es) | 2018-12-14 | 2021-07-07 | Evonik Operations Gmbh | Procedimiento in vitro para la deteccion de disbiosis intestinal aviar. |
| US11684653B2 (en) * | 2019-03-06 | 2023-06-27 | The Cleveland Clinic Foundation | Compositions and method for reducing virulence of microorganisms |
| CN118063554A (zh) | 2020-01-15 | 2024-05-24 | 詹森生物科技公司 | 介白素-23受体的肽抑制剂及其治疗炎性疾病的用途 |
| KR20220141808A (ko) | 2020-01-15 | 2022-10-20 | 얀센 바이오테크 인코포레이티드 | 인터루킨-23 수용체의 펩티드 억제제 및 염증성 질환을 치료하기 위한 이의 용도 |
| KR20230110570A (ko) | 2020-11-20 | 2023-07-24 | 얀센 파마슈티카 엔.브이. | 인터류킨-23 수용체의 펩티드 억제제의 조성물 |
| TW202330013A (zh) | 2021-07-14 | 2023-08-01 | 美商健生生物科技公司 | 介白素－２３受體之脂化肽抑制劑 |
| CN116973572B (zh) * | 2023-06-02 | 2025-03-07 | 东南大学附属中大医院 | 基于血、粪便铁蛋白检测评估肠屏障损伤严重程度的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1577671A1 (en) * | 1996-07-17 | 2005-09-21 | Kaneka Corporation | Diagnostic drugs for autoimmune diseases |
| US6355623B2 (en) * | 1998-09-24 | 2002-03-12 | Hopital-Sainte-Justine | Method of treating IBD/Crohn's disease and related conditions wherein drug metabolite levels in host blood cells determine subsequent dosage |
| US6303321B1 (en) * | 1999-02-11 | 2001-10-16 | North Shore-Long Island Jewish Research Institute | Methods for diagnosing sepsis |
| US7700307B2 (en) * | 2003-03-08 | 2010-04-20 | Auvation Limited | Mitochondrial stress-70 protein markers for colorectal cancer |
| CN101132811B (zh) * | 2004-10-22 | 2012-05-30 | 米迪缪尼有限公司 | 抗hmgb1的高亲和力抗体及其用法 |
| WO2008075788A1 (ja) * | 2006-12-20 | 2008-06-26 | Shino-Test Corporation | ヒトｈｍｇｂ１と特異的に結合する鳥類由来の抗体、ヒトｈｍｇｂ１の免疫学的測定方法及びヒトｈｍｇｂ１の免疫学的測定試薬 |
| AU2008283077A1 (en) * | 2007-08-02 | 2009-02-05 | Ith Immune Therapy Holdings Ab | Diagnosis, staging and monitoring of inflammatory bowel disease |
| JP2011514162A (ja) * | 2008-03-14 | 2011-05-06 | エグザジェン ダイアグノスティクス インコーポレイテッド | 炎症性腸疾患および過敏性腸症候群のバイオマーカー |
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Non-Patent Citations (6)
| Title |
|---|
| JPN6015010946; 'Ｈｍｇｂ１ ｉｓ ａｎ ｅｎｄｏｇｅｎｏｕｓ ｄａｎｇｅｒ ｓｉｇｎａｌ ｉｎ ｉｎｆｌａｍｍａｔｏｒｙ ｂｏｗｅｌ ｄｉｓｅａｓｅ' Ｇａｓｔｒｏｅｎｔｅｒｏｌｏｇｙ １２８（４， Ｓｕｐｐｌ． ２）：Ａ６５１, 2005 * |
| JPN6015010948; Ｅｔｈｙｌ ｐｙｒｕｖａｔｅ ｄｅｃｒｅａｓｅｓ ＨＭＧＢ１ ｒｅｌｅａｓｅ ａｎｄ ａｍｅｌｉｏｒａｔｅｓ ｍｕｒｉｎｅ ｃｏｌｉｔｉｓ: Ｊｏｕｒｎａｌ ｏｆ ｌｅｕｋｏｃｙｔｅ ｂｉｏｌｏｇｙ， （２００９ Ｓｅｐ） Ｖｏｌ． ８６， Ｎｏ． ３， ｐｐ． ６３３-４３ * |
| JPN6015010951; 'Ｅｘｔｒａｃｅｌｌｕｌａｒ Ｈｉｇｈ Ｍｏｂｉｌｉｔｙ Ｇｒｏｕｐ Ｂｏｘ-１ （ＨＭＧＢ１） Ｉｎｈｉｂｉｔｓ Ｅｎｔｅｒｏｃｙｔｅ Ｍｉｇｒａｔｉｏｎ ｖｉａ Ａｃｔｉｖａｔｉｏｎ ｏｆ Ｔｏｌｌ' Ｊ Ｂｉｏｌ Ｃｈｅｍ Ｖｏｌ．２８５ Ｎｏ．７ Ｐａｇｅ．４９９５-５００２ （２０１０．０２．１２） * |
| JPN6015010952; '炎症性腸疾患の発症機序におけるマクロファージの関与：ｍａｃｒｏｐｈａｇｅ ｃｏｌｏｎｙ ｓｔｉｍｕｌａｔｉｎｇ ｆａｃｔｏｒ（Ｍ-ＣＳＦ）の役' 日本消化器病学会雑誌 Ｖｏｌ．１０５ 臨時増刊号 Ｐａｇｅ．Ａ１９９ （２００８．０３．２０） * |
| JPN6015010954; 'Ｆｅｃａｌ ＨＭＧＢ１ Ｉｓ ａ Ｎｏｖｅｌ Ｍａｒｋｅｒ ｏｆ Ｉｎｔｅｓｔｉｎａｌ Ｍｕｃｏｓａｌ Ｉｎｆｌａｍｍａｔｉｏｎ ｉｎ Ｐｅｄｉａｔｒｉｃ Ｉｎｆｌａｍｍａｔｏｒｙ Ｂｏｗｅｌ Ｄｉｓ' Ａｍ Ｊ Ｇａｓｔｒｏｅｎｔｅｒｏｌ , Ｖｏｌ．１０６ Ｎｏ．１１ Ｐａｇｅ．２０２９-２０４０ （２０１１．１１） * |
| JPN7015000718; Ｄａｖｅ， Ｓｈａｉｖａｌ Ｈ: Ｅｘｏｇｅｎｏｕｓ ａｎｄ Ｅｎｄｏｇｅｎｏｕｓ Ｄａｎｇｅｒ Ｓｉｇｎａｌｓ ｉｎ Ｉｎｆｌａｍｍａｔｏｒｙ Ｂｏｗｅｌ Ｄｉｓｅａｓｅ , 2006 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CL2013000223A1 (es) | 2014-03-28 |
| BR112013002145A2 (pt) | 2016-05-24 |
| AU2011287193B2 (en) | 2015-08-13 |
| IL223845A (en) | 2016-06-30 |
| WO2012017466A8 (en) | 2013-07-11 |
| PE20131062A1 (es) | 2013-10-16 |
| EA201390197A1 (ru) | 2013-06-28 |
| EP2601525A1 (en) | 2013-06-12 |
| CA2807107A1 (en) | 2012-02-09 |
| MX2013001327A (es) | 2013-03-08 |
| CN103069276A (zh) | 2013-04-24 |
| AU2011287193A1 (en) | 2013-06-13 |
| IT1406051B1 (it) | 2014-02-06 |
| WO2012017466A1 (en) | 2012-02-09 |
| ITRM20100442A1 (it) | 2012-02-06 |
| US20130137123A1 (en) | 2013-05-30 |
| CA2807107C (en) | 2017-01-03 |
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