JP2013532821A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2013532821A5 JP2013532821A5 JP2013520888A JP2013520888A JP2013532821A5 JP 2013532821 A5 JP2013532821 A5 JP 2013532821A5 JP 2013520888 A JP2013520888 A JP 2013520888A JP 2013520888 A JP2013520888 A JP 2013520888A JP 2013532821 A5 JP2013532821 A5 JP 2013532821A5
- Authority
- JP
- Japan
- Prior art keywords
- substrate
- reagent
- neutral substance
- neutral
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000007935 neutral effect Effects 0.000 claims description 59
- 239000000758 substrate Substances 0.000 claims description 52
- 239000003153 chemical reaction reagent Substances 0.000 claims description 50
- 239000000126 substance Substances 0.000 claims description 48
- 238000000034 method Methods 0.000 claims description 41
- 239000000203 mixture Substances 0.000 claims description 25
- 238000003556 assay Methods 0.000 claims description 21
- 238000003018 immunoassay Methods 0.000 claims description 21
- 239000012070 reactive reagent Substances 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 15
- 238000010168 coupling process Methods 0.000 claims description 13
- 238000005859 coupling reaction Methods 0.000 claims description 13
- 239000004005 microsphere Substances 0.000 claims description 13
- 230000008878 coupling Effects 0.000 claims description 12
- 230000009257 reactivity Effects 0.000 claims description 10
- 239000000427 antigen Substances 0.000 claims description 8
- 108091007433 antigens Proteins 0.000 claims description 8
- 102000036639 antigens Human genes 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- 108010071390 Serum Albumin Proteins 0.000 claims description 7
- 102000007562 Serum Albumin Human genes 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 2
- 239000012491 analyte Substances 0.000 description 11
- 239000000523 sample Substances 0.000 description 6
- 241000894007 species Species 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 239000011248 coating agent Substances 0.000 description 5
- 238000000576 coating method Methods 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 150000003384 small molecules Chemical class 0.000 description 5
- 239000011324 bead Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 108091023037 Aptamer Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical class C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 1
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000012042 active reagent Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000538 analytical sample Substances 0.000 description 1
- -1 antibody Proteins 0.000 description 1
- 238000004630 atomic force microscopy Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 239000007822 coupling agent Substances 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000000572 ellipsometry Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005305 interferometry Methods 0.000 description 1
- 238000001616 ion spectroscopy Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- HZVOZRGWRWCICA-UHFFFAOYSA-N methanediyl Chemical compound [CH2] HZVOZRGWRWCICA-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000007837 multiplex assay Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 239000000583 progesterone congener Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 238000000672 surface-enhanced laser desorption--ionisation Methods 0.000 description 1
- 239000005495 thyroid hormone Substances 0.000 description 1
- 229940036555 thyroid hormone Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36728110P | 2010-07-23 | 2010-07-23 | |
| US61/367,281 | 2010-07-23 | ||
| PCT/US2011/045053 WO2012012748A2 (en) | 2010-07-23 | 2011-07-22 | Co-coupling to control reactivity of reagents in immunoassays |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013532821A JP2013532821A (ja) | 2013-08-19 |
| JP2013532821A5 true JP2013532821A5 (https=) | 2015-06-25 |
| JP5813111B2 JP5813111B2 (ja) | 2015-11-17 |
Family
ID=45494106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013520888A Expired - Fee Related JP5813111B2 (ja) | 2010-07-23 | 2011-07-22 | イムノアッセイにおける試薬の反応性を制御するためのコカップリング |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20120021944A1 (https=) |
| EP (1) | EP2596354B1 (https=) |
| JP (1) | JP5813111B2 (https=) |
| KR (1) | KR101866844B1 (https=) |
| CN (1) | CN103026230B (https=) |
| AU (1) | AU2011280967B2 (https=) |
| BR (1) | BR112013001642A8 (https=) |
| CA (1) | CA2806306A1 (https=) |
| WO (1) | WO2012012748A2 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6479799B2 (ja) * | 2013-08-13 | 2019-03-06 | アンテオ テクノロジーズ プロプライエタリー リミテッドＡｎｔｅｏ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｐｔｙ Ｌｔｄ | 粒子への分子の接合 |
| WO2015106226A2 (en) * | 2014-01-10 | 2015-07-16 | University Of Rochester | Diagnostic device and method for detection of staphylococcus infection |
| ES2732476T3 (es) * | 2014-10-30 | 2019-11-22 | Univ Erasmus Med Ct Rotterdam | Reactivos, métodos y kits para el diagnóstico de inmunodeficiencias primarias |
| JP6218916B1 (ja) * | 2016-12-28 | 2017-10-25 | Jsr株式会社 | 磁性粒子分散液 |
| CN110187132A (zh) * | 2019-05-14 | 2019-08-30 | 太原瑞盛生物科技有限公司 | 一种检测促甲状腺激素的分析方法 |
| CN111879946A (zh) * | 2020-07-28 | 2020-11-03 | 江苏扬新生物医药有限公司 | 一种多指标快速检测荧光免疫层析试剂盒 |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US4357311A (en) * | 1980-10-03 | 1982-11-02 | Warner-Lambert Company | Substrate for immunoassay and means of preparing same |
| EP0281327B1 (en) * | 1987-02-27 | 1993-06-30 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Immunoreactive reagent, method of preparation and its use to determine an immunoreactive species |
| US4812414A (en) * | 1987-09-18 | 1989-03-14 | Eastman Kodak Company | Immunoreactive reagent particles having tracer, receptor molecules and protein of pI less than 6 |
| WO1988007670A2 (en) * | 1987-03-27 | 1988-10-06 | Chimerix Corporation | Time-resolved fluorescence apparatus and immunoassay |
| US5047326A (en) * | 1988-10-07 | 1991-09-10 | Eastman Kodak Company | Immunmological reagent composition and its use in the determination of chlamydial or gonococcal antigens |
| FR2654836B1 (fr) * | 1989-11-17 | 1994-01-28 | Biotrol Sa Laboratoires | Appareil d'execution automatique d'un immunodosage en plusieurs etapes successives d'au moins une substance biologique dans une pluralite d'echantillons biologiques, procede et reactif mettant en óoeuvre ledit appareil. |
| US5143825A (en) * | 1990-03-01 | 1992-09-01 | Abbott Laboratories | Stabilized substrate for use in an immunoassay |
| FR2709492B1 (fr) * | 1993-09-03 | 1995-11-24 | Bioxytech | Méthode d'immunodosage spécifique de la glutathion peroxydase plasmatique humaine, kit pour sa mise en Óoeuvre, oligopeptides et anticorps spécifiques de la méthode . |
| GB9420355D0 (en) * | 1994-10-10 | 1994-11-23 | Univ Nottingham | Preparation of protein microspheres, films and coatings |
| US5736330A (en) | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| WO2001063284A2 (en) * | 2000-02-25 | 2001-08-30 | Luminex Corporation | Internal standards and controls for multiplexed assay |
| US9709559B2 (en) * | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
| US6528321B1 (en) * | 2000-06-26 | 2003-03-04 | Beckman Coulter, Inc. | Opposable-element chromatographic assay device for detection of analytes in whole blood samples |
| FR2855613B1 (fr) * | 2003-05-26 | 2005-08-19 | Biocytex | Procede de detection et de quantification multiplex d'analytes dans un echantillon a l'aide de microspheres |
| US7527980B2 (en) * | 2004-08-18 | 2009-05-05 | Florida State University Research Foundation | Indirect detection of cardiac markers for assessing acute myocardial infarction |
| US7527979B2 (en) * | 2004-08-18 | 2009-05-05 | Florida State University Research Foundation | Devices and methods for rapid detection of pathogens |
| CN101132777A (zh) * | 2004-12-20 | 2008-02-27 | 科利吉姆制药公司 | 用于睡眠障碍的药物组合物 |
| WO2006074076A1 (en) * | 2004-12-31 | 2006-07-13 | Genentech, Inc. | Detecting human antibodies in non-human serum |
| US7396689B2 (en) * | 2005-02-04 | 2008-07-08 | Decision Biomarkers Incorporated | Method of adjusting the working range of a multi-analyte assay |
| WO2007026408A1 (ja) * | 2005-08-30 | 2007-03-08 | Osaka University | 生物学的実験に用いることができる安定な標識媒体 |
| EP1777521A1 (en) * | 2005-10-20 | 2007-04-25 | PENTAX Corporation | Method and kit for the determination of HIV infection |
| NZ575827A (en) * | 2006-09-21 | 2012-02-24 | Prometheus Lab Inc | Antibody-based arrays for detecting multiple signal transducers in rare circulating cells |
| CA2667119C (en) * | 2006-10-18 | 2016-04-12 | Axela Inc. | Measuring multiple analytes over a broad range of concentrations using optical diffraction |
| WO2009091643A1 (en) * | 2008-01-07 | 2009-07-23 | Luminex Corporation | Isolation and enumeration of cells from a complex sample matrix |
| CN101545912A (zh) * | 2008-03-25 | 2009-09-30 | 北京科美东雅生物技术有限公司 | 胰岛素微孔板式磁颗粒化学发光免疫分析测定试剂盒及其制备方法 |
| WO2009152269A2 (en) * | 2008-06-11 | 2009-12-17 | Health Research, Inc. | Methods of quantifying biomarkers |
| EP2350651B1 (en) * | 2008-11-10 | 2015-01-14 | Luminex Corporation | Method for manufacture of macroporous beads |
-
2011
- 2011-07-22 CN CN201180036074.5A patent/CN103026230B/zh not_active Expired - Fee Related
- 2011-07-22 BR BR112013001642A patent/BR112013001642A8/pt not_active IP Right Cessation
- 2011-07-22 KR KR1020137004602A patent/KR101866844B1/ko not_active Expired - Fee Related
- 2011-07-22 EP EP11810481.9A patent/EP2596354B1/en not_active Not-in-force
- 2011-07-22 WO PCT/US2011/045053 patent/WO2012012748A2/en not_active Ceased
- 2011-07-22 AU AU2011280967A patent/AU2011280967B2/en not_active Ceased
- 2011-07-22 CA CA2806306A patent/CA2806306A1/en not_active Abandoned
- 2011-07-22 JP JP2013520888A patent/JP5813111B2/ja not_active Expired - Fee Related
- 2011-07-22 US US13/188,762 patent/US20120021944A1/en not_active Abandoned
-
2013
- 2013-09-20 US US14/032,903 patent/US20140024556A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lee et al. | Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles | |
| US5770457A (en) | Rapid oneside single targeting (ROST) immunoassay method | |
| US20160195521A1 (en) | Analyte Detection | |
| CN104245960B (zh) | 用于生物测定的信号放大的方法和系统 | |
| CA2630544A1 (en) | Multiplex digital immuno-sensing using a library of photocleavable mass tags | |
| WO2014056896A2 (en) | One-step biomolecular immobilisation procedure and products thereof | |
| JP7707237B2 (ja) | 粒子、粒子の製造方法、アフィニティー粒子、及び、これを含む試薬及びキット、並びに標的物質の検出方法 | |
| JP2013532821A5 (https=) | ||
| CN107132359B (zh) | 胃蛋白酶原ⅰ和胃蛋白酶原ⅱ检测方法及其试剂盒 | |
| WO2009066275A1 (en) | A method of immobilising biological molecules to a support and products thereof | |
| JP5813111B2 (ja) | イムノアッセイにおける試薬の反応性を制御するためのコカップリング | |
| Coussot et al. | A rapid and reversible colorimetric assay for the characterization of aminated solid surfaces | |
| JP7399675B2 (ja) | 粒子およびその製造方法 | |
| US7846713B2 (en) | Calibrating microarrays | |
| CN102066899A (zh) | 建立针对生物来源之起始材料中多种分析物的标准品的方法 | |
| JP4984080B2 (ja) | 免疫化学反応のシグナル増強剤および免疫学的測定方法 | |
| EP0903582B1 (en) | Ligand binding surfaces | |
| JP2004117068A (ja) | 免疫測定試薬および免疫測定方法 | |
| Cretich et al. | Peptide microarrays on coated silicon slides for highly sensitive antibody detection | |
| JP2025123943A (ja) | 担体、リガンド結合担体、アフィニティー粒子、及び、これを含む試薬、キット、並びに検出方法 | |
| Morita et al. | Visualization of immunoassay based on a spot test and its application to C-reactive protein | |
| TW202144438A (zh) | 不可逆共價鍵醣蛋白固定方法 |