JP2013504751A5 - - Google Patents
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- Publication number
- JP2013504751A5 JP2013504751A5 JP2012528391A JP2012528391A JP2013504751A5 JP 2013504751 A5 JP2013504751 A5 JP 2013504751A5 JP 2012528391 A JP2012528391 A JP 2012528391A JP 2012528391 A JP2012528391 A JP 2012528391A JP 2013504751 A5 JP2013504751 A5 JP 2013504751A5
- Authority
- JP
- Japan
- Prior art keywords
- urine
- ultrafiltration
- collected
- marker
- gottingen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000002700 Urine Anatomy 0.000 description 4
- 239000003550 marker Substances 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 102100001249 ALB Human genes 0.000 description 1
- 101710027066 ALB Proteins 0.000 description 1
- 102000018358 Immunoglobulins Human genes 0.000 description 1
- 108060003951 Immunoglobulins Proteins 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 229940050528 albumin Drugs 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000000738 capillary electrophoresis-mass spectrometry Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000007374 clinical diagnostic method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 201000011528 vascular disease Diseases 0.000 description 1
Description
1.サンプル調製
診断用のポリペプチドマーカーを検出するために、尿を使用した。健康なドナー(対照群)および血管疾患に罹患している患者から尿を採取した。その後のCE−MS測定のために、限外ろ過によって、患者の尿に高濃度で含まれるタンパク質(アルブミンおよび免疫グロブリンなど)を分離する必要があった。そこで、700μLの尿を採取し、700μlのろ過バッファー(2M尿素、10mMアンモニア、0.02%SDS)と混合した。この体積1.4mLのサンプルを限外ろ過した(20kDa、ザルトリウス社、ドイツ、ゲッティンゲン)。1.1mLの限外ろ液が得られるまで、遠心機を用いて3000rpmで限外ろ過を行った。
診断用のポリペプチドマーカーを検出するために、尿を使用した。健康なドナー(対照群)および血管疾患に罹患している患者から尿を採取した。その後のCE−MS測定のために、限外ろ過によって、患者の尿に高濃度で含まれるタンパク質(アルブミンおよび免疫グロブリンなど)を分離する必要があった。そこで、700μLの尿を採取し、700μlのろ過バッファー(2M尿素、10mMアンモニア、0.02%SDS)と混合した。この体積1.4mLのサンプルを限外ろ過した(20kDa、ザルトリウス社、ドイツ、ゲッティンゲン)。1.1mLの限外ろ液が得られるまで、遠心機を用いて3000rpmで限外ろ過を行った。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09170190.4 | 2009-09-14 | ||
EP09170190 | 2009-09-14 | ||
PCT/EP2010/063475 WO2011029954A2 (de) | 2009-09-14 | 2010-09-14 | Polypeptidmarker zur diagnostik und beurteilung vaskulärer erkrankungen |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2013504751A JP2013504751A (ja) | 2013-02-07 |
JP2013504751A5 true JP2013504751A5 (ja) | 2013-10-24 |
Family
ID=43301867
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012528391A Pending JP2013504751A (ja) | 2009-09-14 | 2010-09-14 | 血管疾患を診断および評価するためのポリペプチドマーカー |
Country Status (4)
Country | Link |
---|---|
US (2) | US20120241321A1 (ja) |
EP (1) | EP2478377A2 (ja) |
JP (1) | JP2013504751A (ja) |
WO (1) | WO2011029954A2 (ja) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2118666B1 (de) * | 2007-03-07 | 2011-08-03 | Mosaiques Diagnostics And Therapeutics AG | Verfahren zur normierung der konzentration von analyten in einer urinprobe |
EP1972940A1 (de) * | 2007-03-14 | 2008-09-24 | mosaiques diagnostics and therapeutics AG | Verfahren und Marker zur Diagnose von Nierenerkrankungen |
US9404932B2 (en) * | 2007-11-05 | 2016-08-02 | Nordic Bioscience A/S | Pathology biomarker assay |
WO2009115570A2 (de) * | 2008-03-19 | 2009-09-24 | Mosaiques Diagnostics And Therapeutics Ag | Verfahren und marker zur diagnose von tubulären nierenschäden und -erkrankungen |
WO2010031822A1 (de) * | 2008-09-17 | 2010-03-25 | Mosaiques Diagnostics And Therapeutics Ag | Nierenzellkarzinom |
WO2013150132A2 (de) * | 2012-04-05 | 2013-10-10 | Mosaiques Diagnostics And Therapeutics Ag | Polypeptidmarker zur diagnostik und beurteilung von schlaganfällen |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20030009413A (ko) | 2000-03-30 | 2003-01-29 | 오르드 레빈슨 | 방뇨 장치 |
DE10021737C2 (de) | 2000-05-04 | 2002-10-17 | Hermann Haller | Verfahren und Vorrichtung zur qualitativen und/oder quantitativen Bestimmung eines Protein- und/oder Peptidmusters einer Flüssigkeitsprobe, die dem menschlichen oder tierischen Körper entnommen wird |
US20100126861A1 (en) * | 2005-11-30 | 2010-05-27 | Harald Mischak | Polypeptide Markers for the Diagnosis and Evaluation of Vascular Diseases |
SG173371A1 (en) * | 2006-07-13 | 2011-08-29 | Univ Iowa Res Found | Methods and reagents for treatment and diagnosis of vascular disorders and age-related macular degeneration |
-
2010
- 2010-09-14 EP EP10775725A patent/EP2478377A2/de not_active Withdrawn
- 2010-09-14 WO PCT/EP2010/063475 patent/WO2011029954A2/de active Application Filing
- 2010-09-14 JP JP2012528391A patent/JP2013504751A/ja active Pending
- 2010-09-14 US US13/395,757 patent/US20120241321A1/en not_active Abandoned
-
2014
- 2014-09-26 US US14/497,613 patent/US20150018246A1/en not_active Abandoned
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