JP2012532612A5 - - Google Patents

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JP2012532612A5
JP2012532612A5 JP2012519765A JP2012519765A JP2012532612A5 JP 2012532612 A5 JP2012532612 A5 JP 2012532612A5 JP 2012519765 A JP2012519765 A JP 2012519765A JP 2012519765 A JP2012519765 A JP 2012519765A JP 2012532612 A5 JP2012532612 A5 JP 2012532612A5
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tissue
slow
cell
aptamer
rate
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JP5926178B2 (ja
JP2012532612A (ja
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JP2012519765A 2009-07-09 2010-07-09 向上したオフ速度を有するアプタマーを生成するための方法 Active JP5926178B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/499,967 2009-07-09
US12/499,967 US7964356B2 (en) 2007-01-16 2009-07-09 Method for generating aptamers with improved off-rates
PCT/US2010/041540 WO2011006075A1 (en) 2009-07-09 2010-07-09 Method for generating aptamers with improved off-rates

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JP2015215544A Division JP2016065872A (ja) 2009-07-09 2015-11-02 向上したオフ速度を有するアプタマーを生成するための方法

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JP2012532612A JP2012532612A (ja) 2012-12-20
JP2012532612A5 true JP2012532612A5 (cg-RX-API-DMAC7.html) 2013-08-29
JP5926178B2 JP5926178B2 (ja) 2016-05-25

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JP2012519765A Active JP5926178B2 (ja) 2009-07-09 2010-07-09 向上したオフ速度を有するアプタマーを生成するための方法
JP2015215544A Pending JP2016065872A (ja) 2009-07-09 2015-11-02 向上したオフ速度を有するアプタマーを生成するための方法

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US (1) US7964356B2 (cg-RX-API-DMAC7.html)
EP (1) EP2451981B1 (cg-RX-API-DMAC7.html)
JP (2) JP5926178B2 (cg-RX-API-DMAC7.html)
AU (1) AU2010271271B2 (cg-RX-API-DMAC7.html)
CA (2) CA2765857C (cg-RX-API-DMAC7.html)
ES (1) ES2610633T3 (cg-RX-API-DMAC7.html)
WO (1) WO2011006075A1 (cg-RX-API-DMAC7.html)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US7964356B2 (en) 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20110136099A1 (en) * 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US8975026B2 (en) * 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7855054B2 (en) * 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US9725713B1 (en) 2007-01-22 2017-08-08 Steven A Benner In vitro selection with expanded genetic alphabets
US8586303B1 (en) 2007-01-22 2013-11-19 Steven Albert Benner In vitro selection with expanded genetic alphabets
US20100151465A1 (en) 2008-03-27 2010-06-17 Jingyue Ju Selective Capture and Release of Analytes
US8404830B2 (en) * 2007-07-17 2013-03-26 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US8703416B2 (en) 2008-07-17 2014-04-22 Somalogic, Inc. Method for purification and identification of sperm cells
US9090663B2 (en) * 2009-04-21 2015-07-28 The Trustees Of Columbia University In The City Of New York Systems and methods for the capture and separation of microparticles
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
JP5905003B2 (ja) 2010-07-09 2016-04-20 ソマロジック・インコーポレーテッド 肺癌バイオマーカーとその使用
WO2012055573A1 (en) * 2010-10-29 2012-05-03 Noxxon Pharma Ag Use of hepcidin binding nucleic acids for depletion of hepcidin from the body
WO2012122540A1 (en) * 2011-03-10 2012-09-13 Somalogic, Inc. Aptamers for clostridium difficile diagnostics
WO2012174496A2 (en) * 2011-06-17 2012-12-20 Somalogic, Inc. Method for purification and identification of sperm cells
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
JP6301258B2 (ja) * 2011-11-03 2018-03-28 トライパス イメージング インコーポレイテッド 免疫染色試料を調製するための方法および構成物
MX355567B (es) 2011-12-30 2018-04-23 Quest Diagnostics Invest Inc Aptameros y metodos de diagnostico para detectar el receptor de egf.
US20150141259A1 (en) 2012-06-07 2015-05-21 Somalogic, Inc. Aptamer-Based Multiplexed Assays
TWI465566B (zh) * 2012-06-07 2014-12-21 Univ Nat Taiwan 具專一性核適體之製備方法及套組
WO2014068553A1 (en) * 2012-10-29 2014-05-08 Yeda Research And Development Co. Ltd. Aptamers, multimeric aptamers and uses thereof
SG11201507335QA (en) * 2013-03-14 2015-10-29 Somalogic Inc Aptamers that bind to il-6 and their use in treating or diagnosing il-6 mediated conditions
ES2834910T3 (es) * 2013-09-09 2021-06-21 Somalogic Inc Aptámeros de PDGF y VEGF que tienen una estabilidad mejorada y su uso en el tratamiento de enfermedades y trastornos mediados por PDGF y VEGF
WO2016022696A1 (en) 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
WO2016123058A1 (en) 2015-01-27 2016-08-04 Somalogic, Inc. Biomarkers for detection of tuberculosis risk
KR102018774B1 (ko) * 2016-03-07 2019-09-04 포항공과대학교 산학협력단 Glypican-3에 선택적으로 결합하는 압타머 및 이의 용도
AU2017235661B2 (en) * 2016-03-18 2023-06-08 Caris Science, Inc. Oligonucleotide probes and uses thereof
CN110049763A (zh) 2016-10-14 2019-07-23 Cns生物科学股份有限公司 治疗脊髓损伤个体的神经性疼痛的方法
JP2020514746A (ja) 2016-12-01 2020-05-21 ノーティラス バイオテクノロジー インコーポレイテッド タンパク質をアッセイする方法
CA3086915A1 (en) 2017-12-29 2019-07-04 Sujal M. Patel Decoding approaches for protein identification
US10481155B2 (en) 2018-03-09 2019-11-19 Somalogic, Inc. Proteomic assay using quantum sensors
WO2019202448A1 (en) 2018-04-16 2019-10-24 University Of Cape Town A three-protein proteomic biomarker for prospective determination of risk for development of active tuberculosis
US20210247387A1 (en) 2018-06-22 2021-08-12 Somalogic, Inc. Improved proteomic multiplex assays
JP7332694B2 (ja) 2018-12-04 2023-08-23 エフ. ホフマン-ラ ロシュ アーゲー 細胞標的の空間指向性量子バーコード化
WO2020132269A1 (en) 2018-12-20 2020-06-25 KSQ Therapeutics, Inc. Substituted pyrazolopyrimidines and substituted purines and their use as ubiquitin-specific-processing protease 1 (usp1) inhibitors
WO2020204151A1 (ja) * 2019-04-04 2020-10-08 株式会社リボミック Fgf9に対するアプタマー及びその使用
JP7323162B2 (ja) * 2019-06-27 2023-08-08 株式会社アドバンジェン 核酸アプタマー
ES3025474T3 (en) 2020-01-15 2025-06-09 Ksq Therapeutics Inc Compositions of substituted pyrazolopyrimidines and uses thereof
WO2021202858A1 (en) * 2020-04-02 2021-10-07 Board Of Regents, The University Of Texas System Rna aptamers and use thereof for treating cancer
CA3182266A1 (en) 2020-06-11 2021-12-16 Vadim Lobanov Methods and systems for computational decoding of biological, chemical, and physical entities
US12181481B2 (en) * 2020-10-29 2024-12-31 Ambergen, Inc. Photocleavable mass-tags for multiplexed mass spectrometric imaging of tissues using biomolecular probes
EP4236958A4 (en) 2020-10-30 2024-09-11 KSQ Therapeutics, Inc. SOLID STATE FORMS OF SUBSTITUTED PYRAZOLOPYRIMIDINES AND THEIR USES
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
US20220227890A1 (en) 2021-01-21 2022-07-21 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
US12037637B2 (en) * 2021-01-25 2024-07-16 The Broad Institute, Inc. Photoselective non-invasive targeted genomic and epigenomic sequencing of spatially-defined cells or subcellular regions
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
WO2022232308A1 (en) 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
EP4399528A1 (en) 2021-09-09 2024-07-17 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
EP4405683A1 (en) 2021-09-22 2024-07-31 Nautilus Subsidiary, Inc. Methods and systems for determining polypeptide interactions
WO2023066881A1 (en) 2021-10-18 2023-04-27 Astrazeneca Ab Inhibition of map3k15 for treating and preventing diabetes
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
EP4441503A1 (en) 2021-11-30 2024-10-09 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
CA3245990A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. INTEGRATED NETWORKS FOR SINGLE-ANALYTE PROCESSES
JP2025512974A (ja) 2022-04-08 2025-04-22 イルミナ インコーポレイテッド アプタマーダイナミックレンジ圧縮及び検出技法
EP4515026A1 (en) 2022-04-25 2025-03-05 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
US20240183858A1 (en) 2022-11-15 2024-06-06 Nautilus Subsidiary, Inc. Standard polypeptides
US20240192202A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. Fluidic media for single-analtye arrays
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
WO2024191806A1 (en) 2023-03-10 2024-09-19 Illumina, Inc. Aptamer detection techniques
EP4689173A1 (en) 2023-03-24 2026-02-11 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
WO2024216233A1 (en) 2023-04-13 2024-10-17 Nautilus Subsidiary, Inc. Artificial proteins for displaying epitopes
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
WO2025006625A1 (en) 2023-06-26 2025-01-02 Illumina, Inc. Interlocking nucleic acids for target hybridization
EP4739992A1 (en) 2023-07-06 2026-05-13 SomaLogic Operating Co., Inc. Detection of target molecules in dried biologic matrices
CN121969766A (zh) 2023-08-31 2026-05-01 因美纳有限公司 适体检测技术
EP4771170A2 (en) 2023-08-31 2026-07-08 Illumina, Inc. Aptamer dynamic range compression and detection techniques
EP4780988A1 (en) 2023-09-19 2026-07-29 SomaLogic Operating Co., Inc. Methods of solid-phase nucleic acid hybridization
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
US20250205706A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
WO2025178951A1 (en) 2024-02-22 2025-08-28 Illumina, Inc. Techniques for dynamic range compression grouping in analyte assays
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
US20250305034A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5599720A (en) 1982-08-27 1997-02-04 Multilyte Limited Measurement of analyte concentration
GB8314523D0 (en) 1983-05-25 1983-06-29 Lowe C R Diagnostic device
US4737453A (en) 1984-12-12 1988-04-12 Immunomedics, Inc. Sandwich immunoassay utilizing a separation specific binding substance
DE3587814T2 (de) 1985-03-30 1994-11-10 Marc Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
US4752566A (en) 1985-12-17 1988-06-21 Genetics Institute, Inc. Displacement polynucleotide method and reagent complex employing labeled probe polynucleotide
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5707796A (en) 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
US5861254A (en) * 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US5750342A (en) 1990-06-11 1998-05-12 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US6001577A (en) 1998-06-08 1999-12-14 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US6127119A (en) 1990-06-11 2000-10-03 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US5789157A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5763177A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
ATE318832T1 (de) 1990-06-11 2006-03-15 Gilead Sciences Inc Verfahren zur vervendung von nukleinsäureliganden
US5705337A (en) 1990-06-11 1998-01-06 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US5962219A (en) 1990-06-11 1999-10-05 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-selex
US5763566A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US5864026A (en) 1990-06-11 1999-01-26 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5712375A (en) 1990-06-11 1998-01-27 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5496938A (en) 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5688935A (en) 1990-06-11 1997-11-18 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US6716580B2 (en) 1990-06-11 2004-04-06 Somalogic, Inc. Method for the automated generation of nucleic acid ligands
US5874218A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound in a substance using a nucleic acid ligand
US5459015A (en) 1990-06-11 1995-10-17 Nexstar Pharmaceuticals, Inc. High-affinity RNA ligands of basic fibroblast growth factor
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
IL101038A0 (en) 1991-02-21 1992-11-15 Gilead Sciences Inc Agent specific for trombin and its use
IE920562A1 (en) 1991-02-21 1992-08-26 Gilead Sciences Aptamer specific for biomolecules and method of making
US5582981A (en) 1991-08-14 1996-12-10 Gilead Sciences, Inc. Method for identifying an oligonucleotide aptamer specific for a target
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
AU4842693A (en) 1992-09-11 1994-04-12 Duke University Method for deriving epitopes
US5428149A (en) 1993-06-14 1995-06-27 Washington State University Research Foundation Method for palladium catalyzed carbon-carbon coulping and products
US5580972A (en) 1993-06-14 1996-12-03 Nexstar Pharmaceuticals, Inc. Purine nucleoside modifications by palladium catalyzed methods
US6458539B1 (en) 1993-09-17 2002-10-01 Somalogic, Inc. Photoselection of nucleic acid ligands
US5614503A (en) 1993-11-12 1997-03-25 Aronex Pharmaceuticals, Inc. Amphipathic nucleic acid transporter
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5658738A (en) * 1994-05-31 1997-08-19 Becton Dickinson And Company Bi-directional oligonucleotides that bind thrombin
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
EP0823914B1 (en) 1995-05-03 2004-08-25 Gilead Sciences, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US6013443A (en) 1995-05-03 2000-01-11 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US5945527A (en) * 1996-05-30 1999-08-31 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modification methods using nucleophiles and carbon monoxide
WO1999027133A1 (en) 1997-11-26 1999-06-03 Medical Research Council Improved selex procedure and an anti-cd4 aptamer
US20060057573A1 (en) 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US6376424B1 (en) 1998-01-21 2002-04-23 Syngenta Crop Protection, Inc. Herbicidal composition
US6403693B2 (en) * 1999-06-18 2002-06-11 The Goodyear Tire & Rubber Company Silica reinforced rubber composition and use in tires
US6897015B2 (en) 2000-03-07 2005-05-24 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of pathogens and biological materials
JP2004500891A (ja) 2000-06-22 2004-01-15 クリノミクス バイオサイエンシズ,インク. 凍結組織マイクロアレイ及びその使用法
US6376190B1 (en) 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
AU2002224401B2 (en) * 2000-10-16 2007-12-06 Gilead Sciences, Inc. Nucleic acid ligands to the prostate specific membrane antigen
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
US20030228603A1 (en) 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same
US7767803B2 (en) 2002-06-18 2010-08-03 Archemix Corp. Stabilized aptamers to PSMA and their use as prostate cancer therapeutics
CA2407825A1 (en) 2002-10-11 2004-04-11 Andrew J. Simmonds Trap-tagging: a novel method for the identification and purification of rna-protein complexes
CA2513072A1 (en) 2003-01-09 2004-07-29 Invitrogen Corporation Cellular delivery and activation polypeptide-nucleic acid complexes
WO2004085049A2 (en) 2003-03-28 2004-10-07 The Regents Of The University Of California Preparation and application of encoded bead aggregates in combinatorial chemistry
US7672786B2 (en) 2003-07-02 2010-03-02 Sergey Krylov Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development
US8062897B2 (en) 2003-07-21 2011-11-22 Aureon Laboratories, Inc. Diagnostic histopathology using multiplex gene expression FISH
EP1584923A3 (en) 2004-04-07 2006-01-04 Roche Diagnostics GmbH Stabilization of biomolecules in samples
AU2005323437B2 (en) 2004-04-30 2011-10-06 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a C5-modified pyrimidine
WO2007044427A2 (en) 2005-10-05 2007-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
HUE025489T2 (en) * 2006-01-17 2016-04-28 Somalogic Inc Multiplexed analysis of test samples
US7855054B2 (en) 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7964356B2 (en) 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
ES3045958T3 (en) 2007-07-17 2025-12-01 Somalogic Operating Co Inc Method for generating aptamers with improved off-rates

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