JP2012520058A - NaVを発現する細胞株とその使用方法 - Google Patents
NaVを発現する細胞株とその使用方法 Download PDFInfo
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Abstract
Description
配列番号20に示されるアミノ酸配列を有するアルファ1サブユニット;
配列番号21に示されるアミノ酸配列を有するアルファ2サブユニット;
配列番号22に示されるアミノ酸配列を有するアルファ3サブユニット;
配列番号23に示されるアミノ酸配列を有するアルファ4サブユニット;
配列番号24に示されるアミノ酸配列を有するアルファ5サブユニット;
配列番号25に示されるアミノ酸配列を有するアルファ7サブユニット;
配列番号26に示されるアミノ酸配列を有するアルファ8サブユニット;
配列番号27に示されるアミノ酸配列を有するアルファ9サブユニット;
配列番号28に示されるアミノ酸配列を有するアルファ10サブユニット;
配列番号29に示されるアミノ酸配列を有するアルファ11サブユニット;
配列番号20〜29のいずれか1つと配列が少なくとも95%同一である、又は実質的に同一であり、電位依存性イオンチャネルを形成することができるポリペプチド;及び
配列番号20〜29のいずれか1つの対立遺伝子変異体であるポリペプチド、からなる群より選択される。
配列番号30に示されるアミノ酸配列を有するベータ1サブユニット;
配列番号31に示されるアミノ酸配列を有するベータ2サブユニット;
配列番号32に示されるアミノ酸配列を有するベータ3サブユニット;
配列番号33に示されるアミノ酸配列を有するベータ4サブユニット;
配列番号30〜33のいずれか1つと配列が少なくとも95%同一又は実質的に同一であり、電位依存性イオンチャネルを調節する可能性があるポリペプチド;及び
配列番号30〜33のいずれか1つの対立遺伝子変異体であるポリペプチド、からなる群より選択される。
Z’ファクター=1−((3σpositive control+3σnegative control)/(μpositive control−μnegative control))
実施例1
NaV1.7ヘテロ三量体を安定して発現する細胞株の作出
発現コンストラクトの作出
pCMV−SCRIPT(Stratagene)及びこれに含まれるCMV及びSV40真核プロモーター;SV40及びHSV−TKポリアデニル化配列;複数のクローニング部位;コザック配列;並びにネオマイシン/カナマイシン耐性カセット(又はアンピシリン、ハイグロマイシン、ピューロマイシン、ゼオシン耐性カセット)などの目的の遺伝子の転写及び翻訳に必要な様々な要素に基づいて、最新式のクローニングを可能にするプラスミド発現ベクターをクローニングした。
ヒトNaV1.7αサブユニット(配列番号13)をコードするプラスミド、ヒトNaV1.7β1サブユニット(配列番号16)をコードするプラスミド及びヒトNaV1.7β2サブユニット(配列番号17)をコードするプラスミドの3つの別個のプラスミドを、標準的な方法を用いて293T細胞に共に導入した(核酸を宿主細胞に導入するために用いられ得る試薬の例にはLIPOFECTAMINE(商標)、LIPOFECTAMINE(商標)2000、OLIGOFECTAMINE(商標)、TFX(商標)試薬、FUGENE(登録商標)6、DOTAP/DOPE、Metafectine又はFECTURIN(商標)が挙げられるがこれらには限定されない)。
以下の配列はNaV1.7サブユニット導入遺伝子のために用いられた。
5’−GTTCTTAAGGCACAGGAACTGGGAC−3’(配列番号1)(NaV1.7αサブユニット)
5’−GAAGTTAACCCTGTCgttctgcgac−3’(配列番号2)(NaV1.7β1サブユニット)
5’−GTTCTATAGGGTCTGCTTGTCGCTC−3’(配列番号3)(NaV1.7β2サブユニット)
100μMのストックとして提供された。
5’−Cy5GCCAGTCCCAGTTCCTGTGCCTTAAGAACCTCGCBHQ3クエンチ−3’(配列番号4)
5’−Cy5.5CGAGTCGCAGAACGACAGGGTTAACTTCCTCGCBHQ3クエンチ−3’(配列番号5)
5’−FamCGAGAGCGACAAGCAGACCCTATAGAACCTCGCBHQ1クエンチ−3’(配列番号34)
ゲーティングの一致→一重ゲート→ゲーティングの確認→FAM対Cy5:生存細胞の0.1〜1.0%プロットにおけるゲーティングの分類
NaV1.7を安定して発現する細胞株における、異種NaV1.7サブユニットの相対的な発現の解析
作成したNaV1.7を安定して発現する細胞株における、異種ヒトNaV1.7α、β1、及びβ2サブユニットの相対的な発現を決定するために、定量的RT−PCR(qRT−PCR)を用いた。RNA抽出用キット(RNeasy Mini Kit、Qiagen)を用いて、1〜3x106の哺乳類細胞からトータルRNAを精製した。DNアーゼ処理プロトコール(TURBO DNA−free Kit、Ambion)に厳密に従って、DNアーゼ処理を行った。逆転写酵素キット(SuperScript III、Invitrogen)を用い、20μL反応液中に1μgのDNAを含まないトータルRNAと250ngのランダムプライマー(Invitrogen)を加えてファーストストランドcDNA合成を行った。逆転写酵素を含まない試料とRNAを含まない試料をこの反応におけるネガティブコントロールとして用いた。25°C5分、50°C60分の条件でサーマルサイクラー(Mastercycler、Eppendorf)を用いて合成を行い、反応の停止は70°Cに15分間置くことにより行った。
電気生理学的アッセイを用いた、天然のNaV機能に対するNaV1.7を安定して発現する細胞株の機能解析
自動化パッチクランプシステムを用いて、作成したNaV1.7α、β1、及びβ2サブユニットを発現する安定なHEK293T細胞株からのナトリウム伝導を記録した。以下に示したプロトコールは、QPatch、Sophion又はPatchliner、Nanionシステムにおいてもまた使用することができる。細胞外のリンガー液には140mM NaCl、4.7mM KCl、2.6mM MgCl2、11mM グルコース及び5mM HEPESが含まれ、室温におけるpHは7.4だった。細胞内のリンガー液には120mM CsF、20mM Cs−EGTA、1mM CaCl2、1mM MgCl2、及び10mM HEPESが含まれ、pHは7.2であった。実験は室温において行った。
膜電位アッセイを用いた、天然のNaV機能に対するNaV1.7を安定して発現する細胞株の機能解析
作成したNaV1.7α、β1、及びβ2サブユニットを発現する安定した細胞を、標準的な条件下で、10%ウシ胎仔血清、グルタミン及びHEPESを加えたダルベッコ改変イーグル培地を用いて維持した。アッセイを行う前日に、例えばCDB(GIBCO)又はcell−stripper(Mediatech)などの細胞分離緩衝液を用いて保存プレートから細胞を回収し、そして増殖培地を加えた384ウェルプレートに、1ウェル当たり10,000〜25,000個の細胞を播種した。アッセイプレートを、37°Cの細胞培養インキュベーター中、5%CO2条件下で22〜24時間維持した。その後アッセイプレートから培地を除去し、そしてロードバッファー(137mM NaCl、5mM KCl、1.25mM CaCl2、25mM HEPES、10mM グルコース)で希釈した青色蛍光膜電位色素(Molecular Devices Inc.)を加えた。細胞を青色蛍光膜電位色素と共に1時間、37℃でインキュベートした。アッセイプレートをその後、ハイスループット蛍光プレートリーダー(Hamamastu FDSS)にセットした。この蛍光プレートリーダーは、セルプレートの画像における細胞の蛍光を1秒に1回測定し、そしてそのデータを相対的な蛍光単位として表示する。
NaV1.7アルファサブユニットのベータサブユニットによる制御の解析
アルファサブユニット遺伝子発現のベータサブユニットによる制御
それぞれのプラスミドDNA又はα及びコントロールプラスミドのモル比を操作することにより、様々な比率のα及びβサブユニットを発現する(例えばα:β1:β2=1:1:1)ようにHEK293T細胞プールを改変した。薬剤選択の後、異なる6つの細胞プールにおけるサブユニットの発現を、実施例2に記載したようにqRT−PCRを用いて評価した。比較qRT−PCRによって、αサブユニットとコントロールプラスミドのみを形質転換した場合(図2、右列)と比較して、3つ全てのヒトNaV1.7サブユニット(すなわちα、β1、及びβ2)を共に形質転換した場合に(図2、左列)、薬剤選抜した細胞において検出されるαサブユニットの発現の上昇が見られた。βサブユニット転写産物の存在がαサブユニットの遺伝子発現に影響を及ぼすことは、機能解析における生理学的な関連には、3つ全てのNaV1.7サブユニットが共に発現することが重要であることを示している。
3つ全てのNaV1.7サブユニット(すなわちα、β1、及びβ2)を安定して共に発現している細胞、及びNaV1.7αサブユニットのみを安定して発現しているコントロール細胞の、試験化合物への反応を測定するためには細胞を用いた膜電位アッセイを用いた。実施例4のプロトコールに実質的に従って行った膜電位アッセイによって、2つの化合物(図5)(すなわちC18及びK21)を試験した。具体的には、この実施例においては試験化合物を最初の添加工程において添加した。
配列表
標的配列1
5’−GTTCTTAAGGCACAGGAACTGGGAC−3’(配列番号1)
標的配列2
5’−GAAGTTAACCCTGTCgttctgcgac−3’(配列番号2)
標的配列3
5’−GTTCTATAGGGTCTGCTTGTCGCTC−3’(配列番号3)
シグナル伝達プローブ1(標的1に結合する)
5’−Cy5GCCAGTCCCAGTTCCTGTGCCTTAAGAACCTCGCBHQ2クエンチ−3’(配列番号4)
シグナル伝達プローブ2−(標的2に結合する)
5’−Cy5.5GCGAGTCGCAGAACGACAGGGTTAACTTCCTCGCBHQ2クエンチ−3’(配列番号5)
ホモ サピエンス(H.s.)SCN1A
atggagcaaacagtgcttgtaccaccaggacctgacagcttcaacttcttcaccagagaatctcttgcggctattgaaagacgcattgcagaagaaaaggcaaagaatcccaaaccagacaaaaaagatgacgacgaaaatggcccaaagccaaatagtgacttggaagctggaaagaaccttccatttatttatggagacattcctccagagatggtgtcagagcccctggaggacctggacccctactatatcaataagaaaacttttatagtattgaataaagggaaggccatcttccggttcagtgccacctctgccctgtacattttaactcccttcaatcctcttaggaaaatagctattaagattttggtacattcattattcagcatgctaattatgtgcactattttgacaaactgtgtgtttatgacaatgagtaaccctcctgattggacaaagaatgtagaatacaccttcacaggaatatatacttttgaatcacttataaaaattattgcaaggggattctgtttagaagattttactttccttcgggatccatggaactggctcgatttcactgtcattacatttgcgtacgtcacagagtttgtggacctgggcaatgtctcggcattgagaacattcagagttctccgagcattgaagacgatttcagtcattccaggcctgaaaaccattgtgggagccctgatccagtctgtgaagaagctctcagatgtaatgatcctgactgtgttctgtctgagcgtatttgctctaattgggctgcagctgttcatgggcaacctgaggaataaatgtatacaatggcctcccaccaatgcttccttggaggaacatagtatagaaaagaatataactgtgaattataatggtacacttataaatgaaactgtctttgagtttgactggaagtcatatattcaagattcaagatatcattatttcctggagggttttttagatgcactactatgtggaaatagctctgatgcaggccaatgtccagagggatatatgtgtgtgaaagctggtagaaatcccaattatggctacacaagctttgataccttcagttgggcttttttgtccttgtttcgactaatgactcaggacttctgggaaaatctttatcaactgacattacgtgctgctgggaaaacgtacatgatattttttgtattggtcattttcttgggctcattctacctaataaatttgatcctggctgtggtggccatggcctacgaggaacagaatcaggccaccttggaagaagcagaacagaaagaggccgaatttcagcagatgattgaacagcttaaaaagcaacaggaggcagctcagcaggcagcaacggcaactgcctcagaacattccagagagcccagtgcagcaggcaggctctcagacagctcatctgaagcctctaagttgagttccaagagtgctaaggaaagaagaaatcggaggaagaaaagaaaacagaaagagcagtctggtggggaagagaaagatgaggatgaattccaaaaatctgaatctgaggacagcatcaggaggaaaggttttcgcttctccattgaagggaaccgattgacatatgaaaagaggtactcctccccacaccagtctttgttgagcatccgtggctccctattttcaccaaggcgaaatagcagaacaagccttttcagctttagagggcgagcaaaggatgtgggatctgagaacgacttcgcagatgatgagcacagcacctttgaggataacgagagccgtagagattccttgtttgtgccccgacgacacggagagagacgcaacagcaacctgagtcagaccagtaggtcatcccggatgctggcagtgtttccagcgaatgggaagatgcacagcactgtggattgcaatggtgtggtttccttggttggtggaccttcagttcctacatcgcctgttggacagcttctgccagagggaacaaccactgaaactgaaatgagaaagagaaggtcaagttctttccacgtttccatggactttctagaagatccttcccaaaggcaacgagcaatgagtatagccagcattctaacaaatacagtagaagaacttgaagaatccaggcagaaatgcccaccctgttggtataaattttccaacatattcttaatctgggactgttctccatattggttaaaagtgaaacatgttgtcaacctggttgtgatggacccatttgttgacctggccatcaccatctgtattgtcttaaatactcttttcatggccatggagcactatccaatgacggaccatttcaataatgtgcttacagtaggaaacttggttttcactgggatctttacagcagaaatgtttctgaaaattattgccatggatccttactattatttccaagaaggctggaatatctttgacggttttattgtgacgcttagcctggtagaacttggactcgccaatgtggaaggattatctgttctccgttcatttcgattgctgcgagttttcaagttggcaaaatcttggccaacgttaaatatgctaataaagatcatcggcaattccgtgggggctctgggaaatttaaccctcgtcttggccatcatcgtcttcatttttgccgtggtcggcatgcagctctttggtaaaagctacaaagattgtgtctgcaagatcgccagtgattgtcaactcccacgctggcacatgaatgacttcttccactccttcctgattgtgttccgcgtgctgtgtggggagtggatagagaccatgtgggactgtatggaggttgctggtcaagccatgtgccttactgtcttcatgatggtcatggtgattggaaacctagtggtcctgaatctctttctggccttgcttctgagctcatttagtgcagacaaccttgcagccactgatgatgataatgaaatgaataatctccaaattgctgtggataggatgcacaaaggagtagcttatgtgaaaagaaaaatatatgaatttattcaacagtccttcattaggaaacaaaagattttagatgaaattaaaccacttgatgatctaaacaacaagaaagacagttgtatgtccaatcatacagcagaaattgggaaagatcttgactatcttaaagatgtaaatggaactacaagtggtataggaactggcagcagtgttgaaaaatacattattgatgaaagtgattacatgtcattcataaacaaccccagtcttactgtgactgtaccaattgctgtaggagaatctgactttgaaaatttaaacacggaagactttagtagtgaatcggatctggaagaaagcaaagagaaactgaatgaaagcagtagctcatcagaaggtagcactgtggacatcggcgcacctgtagaagaacagcccgtagtggaacctgaagaaactcttgaaccagaagcttgtttcactgaaggctgtgtacaaagattcaagtgttgtcaaatcaatgtggaagaaggcagaggaaaacaatggtggaacctgagaaggacgtgtttccgaatagttgaacataactggtttgagaccttcattgttttcatgattctccttagtagtggtgctctggcatttgaagatatatatattgatcagcgaaagacgattaagacgatgttggaatatgctgacaaggttttcacttacattttcattctggaaatgcttctaaaatgggtggcatatggctatcaaacatatttcaccaatgcctggtgttggctggacttcttaattgttgatgtttcattggtcagtttaacagcaaatgccttgggttactcagaacttggagccatcaaatctctcaggacactaagagctctgagacctctaagagccttatctcgatttgaagggatgagggtggttgtgaatgcccttttaggagcaattccatccatcatgaatgtgcttctggtttgtcttatattctggctaattttcagcatcatgggcgtaaatttgtttgctggcaaattctaccactgtattaacaccacaactggtgacaggtttgacatcgaagacgtgaataatcatactgattgcctaaaactaatagaaagaaatgagactgctcgatggaaaaatgtgaaagtaaactttgataatgtaggatttgggtatctctctttgcttcaagttgccacattcaaaggatggatggatataatgtatgcagcagttgattccagaaatgtggaactccagcctaagtatgaagaaagtctgtacatgtatctttactttgttattttcatcatctttgggtccttcttcaccttgaacctgtttattggtgtcatcatagataatttcaaccagcagaaaaagaagtttggaggtcaagacatctttatgacagaagaacagaagaaatactataatgcaatgaaaaaattaggatcgaaaaaaccgcaaaagcctatacctcgaccaggaaacaaatttcaaggaatggtctttgacttcgtaaccagacaagtttttgacataagcatcatgattctcatctgtcttaacatggtcacaatgatggtggaaacagatgaccagagtgaatatgtgactaccattttgtcacgcatcaatctggtgttcattgtgctatttactggagagtgtgtactgaaactcatctctctacgccattattattttaccattggatggaatatttttgattttgtggttgtcattctctccattgtaggtatgtttcttgccgagctgatagaaaagtatttcgtgtcccctaccctgttccgagtgatccgtcttgctaggattggccgaatcctacgtctgatcaaaggagcaaaggggatccgcacgctgctctttgctttgatgatgtcccttcctgcgttgtttaacatcggcctcctactcttcctagtcatgttcatctacgccatctttgggatgtccaactttgcctatgttaagagggaagttgggatcgatgacatgttcaactttgagacctttggcaacagcatgatctgcctattc
caaattacaacctctgctggctgggatggattgctagcacccattctcaacagtaagccacccgactgtgaccctaataaagttaaccctggaagctcagttaagggagactgtgggaacccatctgttggaattttcttttttgtcagttacatcatcatatccttcctggttgtggtgaacatgtacatcgcggtcatcctggagaacttcagtgttgctactgaagaaagtgcagagcctctgagtgaggatgactttgagatgttctatgaggtttgggagaagtttgatcccgatgcaactcagttcatggaatttgaaaaattatctcagtttgcagctgcgcttgaaccgcctctcaatctgccacaaccaaacaaactccagctcattgccatggatttgcccatggtgagtggtgaccggatccactgtcttgatatcttatttgcttttacaaagcgggttctaggagagagtggagagatggatgctctacgaatacagatggaagagcgattcatggcttccaatccttccaaggtctcctatcagccaatcactactactttaaaacgaaaacaagaggaagtatctgctgtcattattcagcgtgcttacagacgccaccttttaaagcgaactgtaaaacaagcttcctttacgtacaataaaaacaaaatcaaaggtggggctaatcttcttataaaagaagacatgataattgacagaataaatgaaaactctattacagaaaaaactgatctgaccatgtccactgcagcttgtccaccttcctatgaccgggtgacaaagccaattgtggaaaaacatgagcaagaaggcaaagatgaaaaagccaaagggaaataa(配列番号6)
H.s.SCN2A
atggcacagtcagtgctggtaccgccaggacctgacagcttccgcttctttaccagggaatcccttgctgctattgaacaacgcattgcagaagagaaagctaagagacccaaacaggaacgcaaggatgaggatgatgaaaatggcccaaagccaaacagtgacttggaagcaggaaaatctcttccatttatttatggagacattcctccagagatggtgtcagtgcccctggaggatctggacccctactatatcaataagaaaacgtttatagtattgaataaagggaaagcaatctctcgattcagtgccacccctgccctttacattttaactcccttcaaccctattagaaaattagctattaagattttggtacattctttattcaatatgctcattatgtgcacgattcttaccaactgtgtatttatgaccatgagtaaccctccagactggacaaagaatgtggagtatacctttacaggaatttatacttttgaatcacttattaaaatacttgcaaggggcttttgtttagaagatttcacatttttacgggatccatggaattggttggatttcacagtcattacttttgcatatgtgacagagtttgtggacctgggcaatgtctcagcgttgagaacattcagagttctccgagcattgaaaacaatttcagtcattccaggcctgaagaccattgtgggggccctgatccagtcagtgaagaagctttctgatgtcatgatcttgactgtgttctgtctaagcgtgtttgcgctaataggattgcagttgttcatgggcaacctacgaaataaatgtttgcaatggcctccagataattcttcctttgaaataaatatcacttccttctttaacaattcattggatgggaatggtactactttcaataggacagtgagcatatttaactgggatgaatatattgaggataaaagtcacttttattttttagaggggcaaaatgatgctctgctttgtggcaacagctcagatgcaggccagtgtcctgaaggatacatctgtgtgaaggctggtagaaaccccaactatggctacacgagctttgacacctttagttgggcctttttgtccttatttcgtctcatgactcaagacttctgggaaaacctttatcaactgacactacgtgctgctgggaaaacgtacatgatattttttgtgctggtcattttcttgggctcattctatctaataaatttgatcttggctgtggtggccatggcctatgaggaacagaatcaggccacattggaagaggctgaacagaaggaagctgaatttcagcagatgctcgaacagttgaaaaagcaacaagaagaagctcaggcggcagctgcagccgcatctgctgaatcaagagacttcagtggtgctggtgggataggagttttttcagagagttcttcagtagcatctaagttgagctccaaaagtgaaaaagagctgaaaaacagaagaaagaaaaagaaacagaaagaacagtctggagaagaagagaaaaatgacagagtccgaaaatcggaatctgaagacagcataagaagaaaaggtttccgtttttccttggaaggaagtaggctgacatatgaaaagagattttcttctccacaccagtccttactgagcatccgtggctcccttttctctccaagacgcaacagtagggcgagccttttcagcttcagaggtcgagcaaaggacattggctctgagaatgactttgctgatgatgagcacagcacctttgaggacaatgacagccgaagagactctctgttcgtgccgcacagacatggagaacggcgccacagcaatgtcagccaggccagccgtgcctccagggtgctccccatcctgcccatgaatgggaagatgcatagcgctgtggactgcaatggtgtggtctccctggtcgggggcccttctaccctcacatctgctgggcagctcctaccagagggcacaactactgaaacagaaataagaaagagacggtccagttcttatcatgtttccatggatttattggaagatcctacatcaaggcaaagagcaatgagtatagccagtattttgaccaacaccatggaagaacttgaagaatccagacagaaatgcccaccatgctggtataaatttgctaatatgtgtttgatttgggactgttgtaaaccatggttaaaggtgaaacaccttgtcaacctggttgtaatggacccatttgttgacctggccatcaccatctgcattgtcttaaatacactcttcatggctatggagcactatcccatgacggagcagttcagcagtgtactgtctgttggaaacctggtcttcacagggatcttcacagcagaaatgtttctcaagataattgccatggatccatattattactttcaagaaggctggaatatttttgatggttttattgtgagccttagtttaatggaacttggtttggcaaatgtggaaggattgtcagttctccgatcattccggctgctccgagttttcaagttggcaaaatcttggccaactctaaatatgctaattaagatcattggcaattctgtgggggctctaggaaacctcaccttggtattggccatcatcgtcttcatttttgctgtggtcggcatgcagctctttggtaagagctacaaagaatgtgtctgcaagatttccaatgattgtgaactcccacgctggcacatgcatgactttttccactccttcctgatcgtgttccgcgtgctgtgtggagagtggatagagaccatgtgggactgtatggaggtcgctggccaaaccatgtgccttactgtcttcatgatggtcatggtgattggaaatctagtggttctgaacctcttcttggccttgcttttgagttccttcagttctgacaatcttgctgccactgatgatgataacgaaatgaataatctccagattgctgtgggaaggatgcagaaaggaatcgattttgttaaaagaaaaatacgtgaatttattcagaaagcctttgttaggaagcagaaagctttagatgaaattaaaccgcttgaagatctaaataataaaaaagacagctgtatttccaaccataccaccatagaaataggcaaagacctcaattatctcaaagacggaaatggaactactagtggcataggcagcagtgtagaaaaatatgtcgtggatgaaagtgattacatgtcatttataaacaaccctagcctcactgtgacagtaccaattgctgttggagaatctgactttgaaaatttaaatactgaagaattcagcagcgagtcagatatggaggaaagcaaagagaagctaaatgcaactagttcatctgaaggcagcacggttgatattggagctcccgccgagggagaacagcctgaggttgaacctgaggaatcccttgaacctgaagcctgttttacagaagactgtgtacggaagttcaagtgttgtcagataagcatagaagaaggcaaagggaaactctggtggaatttgaggaaaacatgctataagatagtggagcacaattggttcgaaaccttcattgtcttcatgattctgctgagcagtggggctctggcctttgaagatatatacattgagcagcgaaaaaccattaagaccatgttagaatatgctgacaaggttttcacttacatattcattctggaaatgctgctaaagtgggttgcatatggttttcaagtgtattttaccaatgcctggtgctggctagacttcctgattgttgatgtctcactggttagcttaactgcaaatgccttgggttactcagaacttggtgccatcaaatccctcagaacactaagagctctgaggccactgagagctttgtcccggtttgaaggaatgagggttgttgtaaatgctcttttaggagccattccatctatcatgaatgtacttctggtttgtctgatcttttggctaatattcagtatcatgggagtgaatctctttgctggcaagttttaccattgtattaattacaccactggagagatgtttgatgtaagcgtggtcaacaactacagtgagtgcaaagctctcattgagagcaatcaaactgccaggtggaaaaatgtgaaagtaaactttgataacgtaggacttggatatctgtctctacttcaagtagccacgtttaagggatggatggatattatgtatgcagctgttgattcacgaaatgtagaattacaacccaagtatgaagacaacctgtacatgtatctttattttgtcatctttattatttttggttcattctttaccttgaatcttttcattggtgtcatcatagataacttcaaccaacagaaaaagaagtttggaggtcaagacatttttatgacagaagaacagaagaaatactacaatgcaatgaaaaaactgggttcaaagaaaccacaaaaacccatacctcgacctgctaacaaattccaaggaatggtctttgattttgtaaccaaacaagtctttgatatcagcatcatgatcctcatctgccttaacatggtcaccatgatggtggaaaccgatgaccagagtcaagaaatgacaaacattctgtactggattaatctggtgtttattgttctgttcactggagaatgtgtgctgaaactgatctctcttcgttactactatttcactattggatggaatatttttgattttgtggtggtcattctctccattgtaggaatgtttctggctgaactgatagaaaagtattttgtgtcccctaccctgttccgagtgatccgtcttgccaggattggccgaatcctacgtctgatcaaaggagcaaaggggatccgcacgctgctctttgctttgatgatgtcccttcctgcgttgtttaacatcggcctccttcttttcctggtcatgttcatctacgccatctttgggatgtccaattttgcctatgttaagagggaagttgggatcgatgacatgttcaactttgagacctttggcaacagcatgatctgcctg
ttccaaattacaacctctgctggctgggatggattgctagcacctattcttaatagtggacctccagactgtgaccctgacaaagatcaccctggaagctcagttaaaggagactgtgggaacccatctgttgggattttcttttttgtcagttacatcatcatatccttcctggttgtggtgaacatgtacatcgcggtcatcctggagaacttcagtgttgctactgaagaaagtgcagagcctctgagtgaggatgactttgagatgttctatgaggtttgggagaagtttgatcccgatgcgacccagtttatagagtttgccaaactttctgattttgcagatgccctggatcctcctcttctcatagcaaaacccaacaaagtccagctcattgccatggatctgcccatggtgagtggtgaccggatccactgtcttgacatcttatttgcttttacaaagcgtgttttgggtgagagtggagagatggatgcccttcgaatacagatggaagagcgattcatggcatcaaacccctccaaagtctcttatgagcccattacgaccacgttgaaacgcaaacaagaggaggtgtctgctattattatccagagggcttacagacgctacctcttgaagcaaaaagttaaaaaggtatcaagtatatacaagaaagacaaaggcaaagaatgtgatggaacacccatcaaagaagatactctcattgataaactgaatgagaattcaactccagagaaaaccgatatgacgccttccaccacgtctccaccctcgtatgatagtgtgaccaaaccagaaaaagaaaaatttgaaaaagacaaatcagaaaaggaagacaaagggaaagatatcagggaaagtaaaaagtaa(配列番号7)
H.s.SCN3A
atggcacaggcactgttggtacccccaggacctgaaagcttccgcctttttactagagaatctcttgctgctatcgaaaaacgtgctgcagaagagaaagccaagaagcccaaaaaggaacaagataatgatgatgagaacaaaccaaagccaaatagtgacttggaagctggaaagaaccttccatttatttatggagacattcctccagagatggtgtcagagcccctggaggacctggatccctactatatcaataagaaaacttttatagtaatgaataaaggaaaggcaattttccgattcagtgccacctctgccttgtatattttaactccactaaaccctgttaggaaaattgctatcaagattttggtacattctttattcagcatgcttatcatgtgcactattttgaccaactgtgtatttatgaccttgagcaaccctcctgactggacaaagaatgtagagtacacattcactggaatctatacctttgagtcacttataaaaatcttggcaagagggttttgcttagaagattttacgtttcttcgtgatccatggaactggctggatttcagtgtcattgtgatggcatatgtgacagagtttgtggacctgggcaatgtctcagcgttgagaacattcagagttctccgagcactgaaaacaatttcagtcattccaggtttaaagaccattgtgggggccctgatccagtcggtaaagaagctttctgatgtgatgatcctgactgtgttctgtctgagcgtgtttgctctcattgggctgcagctgttcatgggcaatctgaggaataaatgtttgcagtggcccccaagcgattctgcttttgaaaccaacaccacttcctactttaatggcacaatggattcaaatgggacatttgttaatgtaacaatgagcacatttaactggaaggattacattggagatgacagtcacttttatgttttggatgggcaaaaagaccctttactctgtggaaatggctcagatgcaggccagtgtccagaaggatacatctgtgtgaaggctggtcgaaaccccaactatggctacacaagctttgacacctttagctgggctttcctgtctctatttcgactcatgactcaagactactgggaaaatctttaccagttgacattacgtgctgctgggaaaacatacatgatattttttgtcctggtcattttcttgggctcattttatttggtgaatttgatcctggctgtggtggccatggcctatgaggagcagaatcaggccaccttggaagaagcagaacaaaaagaggccgaatttcagcagatgctcgaacagcttaaaaagcaacaggaagaagctcaggcagttgcggcagcatcagctgcttcaagagatttcagtggaataggtgggttaggagagctgttggaaagttcttcagaagcatcaaagttgagttccaaaagtgctaaagaatggaggaaccgaaggaagaaaagaagacagagagagcaccttgaaggaaacaacaaaggagagagagacagctttcccaaatccgaatctgaagacagcgtcaaaagaagcagcttccttttctccatggatggaaacagactgaccagtgacaaaaaattctgctcccctcatcagtctctcttgagtatccgtggctccctgttttccccaagacgcaatagcaaaacaagcattttcagtttcagaggtcgggcaaaggatgttggatctgaaaatgactttgctgatgatgaacacagcacatttgaagacagcgaaagcaggagagactcactgtttgtgccgcacagacatggagagcgacgcaacagtaacgttagtcaggccagtatgtcatccaggatggtgccagggcttccagcaaatgggaagatgcacagcactgtggattgcaatggtgtggtttccttggtgggtggaccttcagctctaacgtcacctactggacaacttcccccagagggcaccaccacagaaacggaagtcagaaagagaaggttaagctcttaccagatttcaatggagatgctggaggattcctctggaaggcaaagagccgtgagcatagccagcattctgaccaacacaatggaagaacttgaagaatctagacagaaatgtccgccatgctggtatagatttgccaatgtgttcttgatctgggactgctgtgatgcatggttaaaagtaaaacatcttgtgaatttaattgttatggatccatttgttgatcttgccatcactatttgcattgtcttaaataccctctttatggccatggagcactaccccatgactgagcaattcagtagtgtgttgactgtaggaaacctggtctttactgggattttcacagcagaaatggttctcaagatcattgccatggatccttattactatttccaagaaggctggaatatctttgatggaattattgtcagcctcagtttaatggagcttggtctgtcaaatgtggagggattgtctgtactgcgatcattcagactgcttagagttttcaagttggcaaaatcctggcccacactaaatatgctaattaagatcattggcaattctgtgggggctctaggaaacctcaccttggtgttggccatcatcgtcttcatttttgctgtggtcggcatgcagctctttggtaagagctacaaagaatgtgtctgcaagatcaatgatgactgtacgctcccacggtggcacatgaacgacttcttccactccttcctgattgtgttccgcgtgctgtgtggagagtggatagagaccatgtgggactgtatggaggtcgctggccaaaccatgtgccttattgttttcatgttggtcatggtcattggaaaccttgtggttctgaacctctttctggccttattgttgagttcatttagctcagacaaccttgctgctactgatgatgacaatgaaatgaataatctgcagattgcagtaggaagaatgcaaaagggaattgattatgtgaaaaataagatgcgggagtgtttccaaaaagccttttttagaaagccaaaagttatagaaatccatgaaggcaataagatagacagctgcatgtccaataatactggaattgaaataagcaaagagcttaattatcttagagatgggaatggaaccaccagtggtgtaggtactggaagcagtgttgaaaaatacgtaatcgatgaaaatgattatatgtcattcataaacaaccccagcctcaccgtcacagtgccaattgctgttggagagtctgactttgaaaacttaaatactgaagagttcagcagtgagtcagaactagaagaaagcaaagagaaattaaatgcaaccagctcatctgaaggaagcacagttgatgttgttctaccccgagaaggtgaacaagctgaaactgaacccgaagaagaccttaaaccggaagcttgttttactgaaggatgtattaaaaagtttccattctgtcaagtaagtacagaagaaggcaaagggaagatctggtggaatcttcgaaaaacctgctacagtattgttgagcacaactggtttgagactttcattgtgttcatgatccttctcagtagtggtgcattggcctttgaagatatatacattgaacagcgaaagactatcaaaaccatgctagaatatgctgacaaagtctttacctatatattcattctggaaatgcttctcaaatgggttgcttatggatttcaaacatatttcactaatgcctggtgctggctagatttcttgatcgttgatgtttctttggttagcctggtagccaatgctcttggctactcagaactcggtgccatcaaatcattacggacattaagagctttaagacctctaagagccttatcccggtttgaaggcatgagggtggttgtgaatgctcttgttggagcaattccctctatcatgaatgtgctgttggtctgtctcatcttctggttgatctttagcatcatgggtgtgaatttgtttgctggcaagttctaccactgtgttaacatgacaacgggtaacatgtttgacattagtgatgttaacaatttgagtgactgtcaggctcttggcaagcaagctcggtggaaaaacgtgaaagtaaactttgataatgttggcgctggctatcttgcactgcttcaagtggccacatttaaaggctggatggatattatgtatgcagctgttgattcacgagatgttaaacttcagcctgtatatgaagaaaatctgtacatgtatttatactttgtcatctttatcatctttgggtcattcttcactctgaatctattcattggtgtcatcatagataacttcaaccagcagaaaaagaagtttggaggtcaagacatctttatgacagaggaacagaaaaaatattacaatgcaatgaagaaacttggatccaagaaacctcagaaacccatacctcgcccagcaaacaaattccaaggaatggtctttgattttgtaaccagacaagtctttgatatcagcatcatgatcctcatctgcctcaacatggtcaccatgatggtggaaacggatgaccagggcaaatacatgaccctagttttgtcccggatcaacctagtgttcattgttctgttcactggagaatttgtgctgaagctcgtctccctcagacactactacttcactataggctggaacatctttgactttgtggtggtgattctctccattgtaggtatgtttctggctgagatgatagaaaagtattttgtgtcccctaccttgttccgagtgatccgtcttgccaggattggccgaatcctacgtctgatcaaaggagcaaaggggatccgcacgctgctctttgctttgatgatgtcccttcctgcgttgtttaacatcggcctcctgctcttcctggtcatgtttatctatgccatctttgggatgtccaactttgcctatgttaaaaaggaagctggaattgatgacatgttcaactttgagacctttggcaacagcatgatctgcttgttccaaattacaacc
tctgctggctgggatggattgctagcacctattcttaatagtgcaccacccgactgtgaccctgacacaattcaccctggcagctcagttaagggagactgtgggaacccatctgttgggattttcttttttgtcagttacatcatcatatccttcctggttgtggtgaacatgtacatcgcggtcatcctggagaacttcagtgttgctactgaagaaagtgcagagcccctgagtgaggatgactttgagatgttctatgaggtttgggaaaagtttgatcccgatgcgacccagtttatagagttctctaaactctctgattttgcagctgccctggatcctcctcttctcatagcaaaacccaacaaagtccagcttattgccatggatctgcccatggtcagtggtgaccggatccactgtcttgatattttatttgcctttacaaagcgtgttttgggtgagagtggagagatggatgcccttcgaatacagatggaagacaggtttatggcatcaaacccctccaaagtctcttatgagcctattacaaccactttgaaacgtaaacaagaggaggtgtctgccgctatcattcagcgtaatttcagatgttatcttttaaagcaaaggttaaaaaatatatcaagtaactataacaaagaggcaattaaagggaggattgacttacctataaaacaagacatgattattgacaaactaaatgggaactccactccagaaaaaacagatgggagttcctctaccacctctcctccttcctatgatagtgtaacaaaaccagacaaggaaaagtttgagaaagacaaaccagaaaaagaaagcaaaggaaaagaggtcagagaaaatcaaaagtaa(配列番号8)
H.s.SCN4A
atggccagaccatctctgtgcaccctggtgcctctgggccctgagtgcttgcgccccttcacccgggagtcactggcagccatagaacagcgggcggtggaggaggaggcccggctgcagcggaataagcagatggagattgaggagcccgaacggaagccacgaagtgacttggaggctggcaagaacctacccatgatctacggagaccccccgccggaggtcatcggcatccccctggaggacctggatccctactacagcaataagaagaccttcatcgtactcaacaagggcaaggccatcttccgcttctccgccacacctgctctctacctgctgagccccttcagcgtagtcaggcgcggggccatcaaggtgctcatccatgcgctgttcagcatgttcatcatgatcaccatcttgaccaactgcgtattcatgaccatgagtgacccgcctccctggtccaagaatgtggagtacaccttcacagggatctacacctttgagtccctcatcaagatactggcccgaggcttctgtgtcgacgacttcacattcctccgggacccctggaactggctggacttcagtgtcatcatgatggcgtacctgacagagtttgtggacttgggcaacatctcagccctgaggaccttccgggtgctgcgggccctcaaaaccatcacggtcatcccagggctgaagacgatcgtgggggccctgatccagtcggtgaaaaagctgtcggatgtgatgatcctcactgtcttctgcctgagcgtctttgcgctggtaggactgcagctcttcatgggaaacctgaggcagaagtgtgtgcgctggcccccgccgttcaacgacaccaacaccacgtggtacagcaatgacacgtggtacggcaatgacacatggtatggcaatgagatgtggtacggcaatgactcatggtatgccaacgacacgtggaacagccatgcaagctgggccaccaacgatacctttgattgggacgcctacatcagtgatgaagggaacttctacttcctggagggctccaacgatgccctgctctgtgggaacagcagtgatgctgggcactgccctgagggttatgagtgcatcaagaccgggcggaaccccaactatggctacaccagctatgacaccttcagctgggccttcttggctctcttccgcctcatgacacaggactattgggagaacctcttccagctgacccttcgagcagctggcaagacctacatgatcttcttcgtggtcatcatcttcctgggctctttctacctcatcaatctgatcctggccgtggtggccatggcatatgccgagcagaatgaggccaccctggccgaggataaggagaaagaggaggagtttcagcagatgcttgagaagttcaaaaagcaccaggaggagctggagaaggccaaggccgcccaagctctggaaggtggggaggcagatggggacccagcccatggcaaagactgcaatggcagcctggacacatcgcaaggggagaagggagccccgaggcagagcagcagcggagacagcggcatctccgacgccatggaagaactggaagaggcccaccaaaagtgcccaccatggtggtacaagtgcgcccacaaagtgctcatatggaactgctgcgccccgtggctgaagttcaagaacatcatccacctgatcgtcatggacccgttcgtggacctgggcatcaccatctgcatcgtgctcaacaccctcttcatggccatggaacattaccccatgacggagcactttgacaacgtgctcactgtgggcaacctggtcttcacaggcatcttcacagcagagatggttctgaagctgattgccatggacccctacgagtatttccagcagggttggaatatcttcgacagcatcatcgtcaccctcagcctggtagagctaggcctggccaacgtacagggactgtctgtgctacgctccttccgtctgctgcgggtcttcaagctggccaagtcgtggccaacgctgaacatgctcatcaagatcattggcaattcagtgggggcgctgggtaacctgacgctggtgctggctatcatcgtgttcatcttcgccgtggtgggcatgcagctgtttggcaagagctacaaggagtgcgtgtgcaagattgccttggactgcaacctgccgcgctggcacatgcatgatttcttccactccttcctcatcgtcttccgcatcctgtgcggggagtggatcgagaccatgtgggactgcatggaggtggccggccaagccatgtgcctcaccgtcttcctcatggtcatggtcatcggcaatcttgtggtcctgaacctgttcctggctctgctgctgagctccttcagcgccgacagtctggcagcctcggatgaggatggcgagatgaacaacctgcagattgccatcgggcgcatcaagttgggcatcggctttgccaaggccttcctcctggggctgctgcatggcaagatcctgagccccaaggacatcatgctcagcctcggggaggctgacggggccggggaggctggagaggcgggggagactgcccccgaggatgagaagaaggagccgcccgaggaggacctgaagaaggacaatcacatcctgaaccacatgggcctggctgacggccccccatccagcctcgagctggaccaccttaacttcatcaacaacccctacctgaccatacaggtgcccatcgcctccgaggagtccgacctggagatgcccaccgaggaggaaaccgacactttctcagagcctgaggatagcaagaagccgccgcagcctctctatgatgggaactcgtccgtctgcagcacagctgactacaagccccccgaggaggaccctgaggagcaggcagaggagaaccccgagggggagcagcctgaggagtgcttcactgaggcctgcgtgcagcgctggccctgcctctacgtggacatctcccagggccgtgggaagaagtggtggactctgcgcagggcctgcttcaagattgtcgagcacaactggttcgagaccttcattgtcttcatgatcctgctcagcagtggggctctggccttcgaggacatctacattgagcagcggcgagtcattcgcaccatcctagaatatgccgacaaggtcttcacctacatcttcatcatggagatgctgctcaaatgggtggcctacggctttaaggtgtacttcaccaacgcctggtgctggctcgacttcctcatcgtggatgtctccatcatcagcttggtggccaactggctgggctactcggagctgggacccatcaaatccctgcggacactgcgggccctgcgtcccctgagggcactgtcccgattcgagggcatgagggtggtggtgaacgccctcctaggcgccatcccctccatcatgaatgtgctgcttgtctgcctcatcttctggctgatcttcagcatcatgggtgtcaacctgtttgccggcaagttctactactgcatcaacaccaccacctctgagaggttcgacatctccgaggtcaacaacaagtctgagtgcgagagcctcatgcacacaggccaggtccgctggctcaatgtcaaggtcaactacgacaacgtgggtctgggctacctctccctcctgcaggtggccaccttcaagggttggatggacatcatgtatgcagccgtggactcccgggagaaggaggagcagccgcagtacgaggtgaacctctacatgtacctctactttgtcatcttcatcatctttggctccttcttcaccctcaacctcttcattggcgtcatcattgacaacttcaaccagcagaagaagaagttaggggggaaagacatctttatgacggaggaacagaagaaatactataacgccatgaagaagcttggctccaagaagcctcagaagccaattccccggccccagaacaagatccagggcatggtgtatgacctcgtgacgaagcaggccttcgacatcaccatcatgatcctcatctgcctcaacatggtcaccatgatggtggagacagacaaccagagccagctcaaggtggacatcctgtacaacatcaacatgatcttcatcatcatcttcacaggggagtgcgtgctcaagatgctcgccctgcgccagtactacttcaccgttggctggaacatctttgacttcgtggtcgtcatcctgtccattgtgggccttgccctctctgacctgatccagaagtacttcgtgtcacccacgctgttccgtgtgatccgcctggcgcggattgggcgtgtcctgcggctgatccgcggggccaagggcatccggacgctgctgttcgccctcatgatgtcgctgcctgccctcttcaacatcggcctcctcctcttcctggtcatgttcatctactccatcttcggcatgtccaactttgcctacgtcaagaaggagtcgggcatcgatgatatgttcaacttcgagaccttcggcaacagcatcatctgcctgttcgagatcaccacgtcggccggctgggacgggctcctcaaccccatcctcaacagcgggcccccagactgtgaccccaacctggagaacccgggcaccagtgtcaagggtgactgcggcaacccctccatcggcatctgcttcttctgcagctatatcatcatctccttcctcatcgtggtcaacatgtacatcgccatcatcctggagaacttcaatgtggccacagaggagagcagcgagccccttggtgaagatgactttgagatgttctacgagacatgggagaagttcgaccccgacgccacccagttcatcgcctacagccgcctctcagacttcgtggacaccctgcaggaaccgctgaggattgccaagcccaacaagatcaagctcatcacactggacttgcccatggtgccaggggacaagatccactgcctggacatcctctttgccctgaccaaagaggtcctgggtgactctggggaaatggacgccctcaagcagaccatggaggagaagttcatggca
gccaacccctccaaggtgtcctacgagcccatcaccaccaccctcaagaggaagcacgaggaggtgtgcgccatcaagatccagagggcctaccgccggcacctgctacagcgctccatgaagcaggcatcctacatgtaccgccacagccacgacggcagcggggatgacgcccctgagaaggaggggctgcttgccaacaccatgagcaagatgtatggccacgagaatgggaacagcagctcgccaagcccggaggagaagggcgaggcaggggacgccggacccactatggggctgatgcccatcagcccctcagacactgcctggcctcccgcccctcccccagggcagactgtgcgcccaggtgtcaaggagtctcttgtctag(配列番号9)
H.s.SCN5A
atggcaaacttcctattacctcggggcaccagcagcttccgcaggttcacacgggagtccctggcagccatcgagaagcgcatggcagagaagcaagcccgcggctcaaccaccttgcaggagagccgagaggggctgcccgaggaggaggctccccggccccagctggacctgcaggcctccaaaaagctgccagatctctatggcaatccaccccaagagctcatcggagagcccctggaggacctggaccccttctatagcacccaaaagactttcatcgtactgaataaaggcaagaccatcttccggttcagtgccaccaacgccttgtatgtcctcagtcccttccaccccatccggagagcggctgtgaagattctggttcactcgctcttcaacatgctcatcatgtgcaccatcctcaccaactgcgtgttcatggcccagcacgaccctccaccctggaccaagtatgtcgagtacaccttcaccgccatttacacctttgagtctctggtcaagattctggctcgaggcttctgcctgcacgcgttcactttccttcgggacccatggaactggctggactttagtgtgattatcatggcatacacaactgaatttgtggacctgggcaatgtctcagccttacgcaccttccgagtcctccgggccctgaaaactatatcagtcatttcagggctgaagaccatcgtgggggccctgatccagtctgtgaagaagctggctgatgtgatggtcctcacagtcttctgcctcagcgtctttgccctcatcggcctgcagctcttcatgggcaacctaaggcacaagtgcgtgcgcaacttcacagcgctcaacggcaccaacggctccgtggaggccgacggcttggtctgggaatccctggacctttacctcagtgatccagaaaattacctgctcaagaacggcacctctgatgtgttactgtgtgggaacagctctgacgctgggacatgtccggagggctaccggtgcctaaaggcaggcgagaaccccgaccacggctacaccagcttcgattcctttgcctgggcctttcttgcactcttccgcctgatgacgcaggactgctgggagcgcctctatcagcagaccctcaggtccgcagggaagatctacatgatcttcttcatgcttgtcatcttcctggggtccttctacctggtgaacctgatcctggccgtggtcgcaatggcctatgaggagcaaaaccaagccaccatcgctgagaccgaggagaaggaaaagcgcttccaggaggccatggaaatgctcaagaaagaacacgaggccctcaccatcaggggtgtggataccgtgtcccgtagctccttggagatgtcccctttggccccagtaaacagccatgagagaagaagcaagaggagaaaacggatgtcttcaggaactgaggagtgtggggaggacaggctccccaagtctgactcagaagatggtcccagagcaatgaatcatctcagcctcacccgtggcctcagcaggacttctatgaagccacgttccagccgcgggagcattttcacctttcgcaggcgagacctgggttctgaagcagattttgcagatgatgaaaacagcacagcgggggagagcgagagccaccacacatcactgctggtgccctggcccctgcgccggaccagtgcccagggacagcccagtcccggaacctcggctcctggccacgccctccatggcaaaaagaacagcactgtggactgcaatggggtggtctcattactgggggcaggcgacccagaggccacatccccaggaagccacctcctccgccctgtgatgctagagcacccgccagacacgaccacgccatcggaggagccaggcgggccccagatgctgacctcccaggctccgtgtgtagatggcttcgaggagccaggagcacggcagcgggccctcagcgcagtcagcgtcctcaccagcgcactggaagagttagaggagtctcgccacaagtgtccaccatgctggaaccgtctcgcccagcgctacctgatctgggagtgctgcccgctgtggatgtccatcaagcagggagtgaagttggtggtcatggacccgtttactgacctcaccatcactatgtgcatcgtactcaacacactcttcatggcgctggagcactacaacatgacaagtgaattcgaggagatgctgcaggtcggaaacctggtcttcacagggattttcacagcagagatgaccttcaagatcattgccctcgacccctactactacttccaacagggctggaacatcttcgacagcatcatcgtcatccttagcctcatggagctgggcctgtcccgcatgagcaacttgtcggtgctgcgctccttccgcctgctgcgggtcttcaagctggccaaatcatggcccaccctgaacacactcatcaagatcatcgggaactcagtgggggcactggggaacctgacactggtgctagccatcatcgtgttcatctttgctgtggtgggcatgcagctctttggcaagaactactcggagctgagggacagcgactcaggcctgctgcctcgctggcacatgatggacttctttcatgccttcctcatcatcttccgcatcctctgtggagagtggatcgagaccatgtgggactgcatggaggtgtcggggcagtcattatgcctgctggtcttcttgcttgttatggtcattggcaaccttgtggtcctgaatctcttcctggccttgctgctcagctccttcagtgcagacaacctcacagcccctgatgaggacagagagatgaacaacctccagctggccctggcccgcatccagaggggcctgcgctttgtcaagcggaccacctgggatttctgctgtggtctcctgcggcagcggcctcagaagcccgcagcccttgccgcccagggccagctgcccagctgcattgccaccccctactccccgccacccccagagacggagaaggtgcctcccacccgcaaggaaacacggtttgaggaaggcgagcaaccaggccagggcacccccggggatccagagcccgtgtgtgtgcccatcgctgtggccgagtcagacacagatgaccaagaagaagatgaggagaacagcctgggcacggaggaggagtccagcaagcagcaggaatcccagcctgtgtccggtggcccagaggcccctccggattccaggacctggagccaggtgtcagcgactgcctcctctgaggccgaggccagtgcatctcaggccgactggcggcagcagtggaaagcggaaccccaggccccagggtgcggtgagaccccagaggacagttgctccgagggcagcacagcagacatgaccaacaccgctgagctcctggagcagatccctgacctcggccaggatgtcaaggacccagaggactgcttcactgaaggctgtgtccggcgctgtccctgctgtgcggtggacaccacacaggccccagggaaggtctggtggcggttgcgcaagacctgctaccacatcgtggagcacagctggttcgagacattcatcatcttcatgatcctactcagcagtggagcgctggccttcgaggacatctacctagaggagcggaagaccatcaaggttctgcttgagtatgccgacaagatgttcacatatgtcttcgtgctggagatgctgctcaagtgggtggcctacggcttcaagaagtacttcaccaatgcctggtgctggctcgacttcctcatcgtagacgtctctctggtcagcctggtggccaacaccctgggctttgccgagatgggccccatcaagtcactgcggacgctgcgtgcactccgtcctctgagagctctgtcacgatttgagggcatgagggtggtggtcaatgccctggtgggcgccatcccgtccatcatgaacgtcctcctcgtctgcctcatcttctggctcatcttcagcatcatgggcgtgaacctctttgcggggaagtttgggaggtgcatcaaccagacagagggagacttgcctttgaactacaccatcgtgaacaacaagagccagtgtgagtccttgaacttgaccggagaattgtactggaccaaggtgaaagtcaactttgacaacgtgggggccgggtacctggcccttctgcaggtggcaacatttaaaggctggatggacattatgtatgcagctgtggactccagggggtatgaagagcagcctcagtgggaatacaacctctacatgtacatctattttgtcattttcatcatctttgggtctttcttcaccctgaacctctttattggtgtcatcattgacaacttcaaccaacagaagaaaaagttagggggccaggacatcttcatgacagaggagcagaagaagtactacaatgccatgaagaagctgggctccaagaagccccagaagcccatcccacggcccctgaacaagtaccagggcttcatattcgacattgtgaccaagcaggcctttgacgtcaccatcatgtttctgatctgcttgaatatggtgaccatgatggtggagacagatgaccaaagtcctgagaaaatcaacatcttggccaagatcaacctgctctttgtggccatcttcacaggcgagtgtattgtcaagctggctgccctgcgccactactacttcaccaacagctggaatatcttcgacttcgtggttgtcatcctctccatcgtgggcactgtgctctcggacatcatccagaagtacttcttctccccgacgctcttccgagtcatccgcctggcccgaataggccgcatcctcagactgatccgaggggccaaggggatccgcacgctgctctttgccctcatgatgtccctgcctgccctcttcaacatcgggctgctgctcttcctcgtcatgttcatctactccatctttggcatggccaacttcgcttatgtcaagtgggaggctggcatcgacgacatgttcaacttccagaccttcgccaacagcatgctgtgcctcttccagatc
accacgtcggccggctgggatggcctcctcagccccatcctcaacactgggccgccctactgcgaccccactctgcccaacagcaatggctctcggggggactgcgggagcccagccgtgggcatcctcttcttcaccacctacatcatcatctccttcctcatcgtggtcaacatgtacattgccatcatcctggagaacttcagcgtggccacggaggagagcaccgagcccctgagtgaggacgacttcgatatgttctatgagatctgggagaaatttgacccagaggccactcagtttattgagtattcggtcctgtctgactttgccgatgccctgtctgagccactccgtatcgccaagcccaaccagataagcctcatcaacatggacctgcccatggtgagtggggaccgcatccattgcatggacattctctttgccttcaccaaaagggtcctgggggagtctggggagatggacgccctgaagatccagatggaggagaagttcatggcagccaacccatccaagatctcctacgagcccatcaccaccacactccggcgcaagcacgaagaggtgtcggccatggttatccagagagccttccgcaggcacctgctgcaacgctctttgaagcatgcctccttcctcttccgtcagcaggcgggcagcggcctctccgaagaggatgcccctgagcgagagggcctcatcgcctacgtgatgagtgagaacttctcccgaccccttggcccaccctccagctcctccatctcctccacttccttcccaccctcctatgacagtgtcactagagccaccagcgataacctccaggtgcgggggtctgactacagccacagtgaagatctcgccgacttccccccttctccggacagggaccgtgagtccatcgtgtga(配列番号10)
H.s.SCN7A
atgttggcttcaccagaacctaagggccttgttcccttcactaaagagtcttttgaacttataaaacagcatattgctaaaacacataatgaagaccatgaagaagaagacttaaagccaactcctgatttggaagttggcaaaaagcttccatttatttatggaaacctttctcaaggaatggtgtcagagcccttggaagatgtggacccatattactacaagaaaaaaaatactttcatagtattaaataaaaatagaacaatcttcagattcaatgcggcttccatcttgtgtacattgtctcctttcaattgtattagaagaacaactatcaaggttttggtacatccctttttccaactgtttattctaattagtgtcctgattgattgcgtattcatgtccctgactaatttgccaaaatggagaccagtattagagaatactttgcttggaatttacacatttgaaatacttgtaaaactctttgcaagaggtgtctgggcaggatcattttccttcctcggtgatccatggaactggctcgatttcagcgtaactgtgtttgaggttattataagatactcacctctggacttcattccaacgcttcaaactgcaagaactttgagaattttaaaaattattcctttaaatcaaggtctgaaatcccttgtaggggtcctgatccactgcttgaagcagcttattggtgtcattatcctaactctgttttttctgagcatattttctctaattgggatggggctcttcatgggcaacttgaaacataaatgttttcgatggccccaagagaatgaaaatgaaaccctgcacaacagaactggaaacccatattatattcgagaaacagaaaacttttattatttggaaggagaaagatatgctctcctttgtggcaacaggacagatgctggtcagtgtcctgaaggatatgtgtgtgtaaaagctggcataaatcctgatcaaggcttcacaaattttgacagttttggctgggccttatttgccctatttcggttaatggctcaggattaccctgaagtactttatcaccagatactttatgcttctgggaaggtctacatgatattttttgtggtggtaagttttttgttttccttttatatggcaagtttgttcttaggcatacttgccatggcctatgaagaagaaaagcagagagttggtgaaatatctaagaagattgaaccaaaatttcaacagactggaaaagaacttcaagaaggaaatgaaacagatgaggccaagaccatacaaatagaaatgaagaaaaggtcaccaatttccacagacacatcattggatgtgttggaagatgctactctcagacataaggaagaacttgaaaaatccaagaagatatgcccattatactggtataagtttgctaaaactttcttgatctggaattgttctccctgttggttaaaattgaaagagtttgtccataggattataatggcaccatttactgatcttttccttatcatatgcataattttaaacgtatgttttctgaccttggagcattatccaatgagtaaacaaactaacactcttctcaacattggaaacctggttttcattggaattttcacagcagaaatgatttttaaaataattgcaatgcatccatatgggtatttccaagtaggttggaacatttttgatagcatgatagtgttccatggtttaatagaactttgtctagcaaatgttgcaggaatggctcttcttcgattattcaggatgttaagaattttcaagttgggaaagtattggccaacattccagattttgatgtggtctcttagtaactcatgggtggccctgaaagacttggtcctgttgttgttcacattcatcttcttttctgctgcattcggcatgaagctgtttggtaagaattatgaagaatttgtctgccacatagacaaagactgtcaactcccacgctggcacatgcatgactttttccactccttcctgaatgtgttccgaattctctgtggagagtgggtagagaccttgtgggactgtatggaggttgcaggccaatcctggtgtattcctttttacctgatggtcattttaattggaaatttactggtactttacctgtttctggcattggtgagctcatttagttcatgcaaggatgtaacagctgaagagaataatgaagcaaaaaatctccagcttgcagtggcaagaattaaaaaaggaataaactatgtgcttcttaaaatactatgcaaaacacaaaatgtcccaaaggacacaatggaccatgtaaatgaggtatatgttaaagaagatatttctgaccataccctttctgaattgagcaacacccaagattttctcaaagataaggaaaaaagcagtggcacagagaaaaacgctactgaaaatgagagccaatcacttatccccagtcctagtgtctcagaaactgtaccaattgcttcaggagaatctgatatagaaaatctggataataaggagattcagagtaagtctggtgatggaggcagcaaagagaaaataaagcaatctagctcatctgaatgcagtactgttgatattgctatctctgaagaagaagaaatgttctatggaggtgaaagatcaaagcatctgaaaaatggttgcagacgcggatcttcacttggtcaaatcagtggagcatccaagaaaggaaaaatctggcagaacatcaggaaaacctgctgcaagattgtagagaacaattggtttaagtgttttattgggcttgttactctgctcagcactggcactctggcttttgaagatatatatatggatcagagaaagacaattaaaattttattagaatatgctgacatgatctttacttatatcttcattctggaaatgcttctaaaatggatggcatatggttttaaggcctatttctctaatggctggtacaggctggacttcgtggttgttattgtgttttgtcttagcttaataggcaaaactcgggaagaactaaaacctcttatttccatgaaattccttcggcccctcagagttctatctcaatttgaaagaatgaaggtggttgtgagagctttgatcaaaacaaccttacccactttgaatgtgtttcttgtctgcctgatgatctggctgatttttagtatcatgggagtagacttatttgctggcagattctatgaatgcattgacccaacaagtggagaaaggtttccttcatctgaagtcatgaataagagtcggtgtgaaagccttctgtttaacgaatccatgctatgggaaaatgcaaaaatgaactttgataatgttggaaatggtttcctttctctgcttcaagtagcaacatttaatggatggatcactattatgaattcagcaattgattctgttgctgttaatatacagcctcattttgaagtcaacatctacatgtattgttactttatcaactttattatatttggagtatttctccctctgagtatgctgattactgttattattgataatttcaacaagcataaaataaagctgggaggctcaaatatctttataacggttaaacagagaaaacagtaccgcaggctgaagaagctaatgtatgaggattctcaaagaccagtacctcgcccattaaacaagctccaaggattcatctttgatgtggtaacaagccaagcttttaatgtcattgttatggttcttatatgtttccaagcaatagccatgatgatagacactgatgttcagagtctacaaatgtccattgctctctactggattaactcaatttttgttatgctatatactatggaatgtatactgaagctcatcgctttccgttgtttttatttcaccattgcgtggaacatttttgattttatggtggttattttctccatcacaggactatgtctgcctatgacagtaggatcctaccttgtgcctccttcacttgtgcaactgatacttctctcacggatcattcacatgctgcgtcttggaaaaggaccaaaggtgtttcataatctgatgcttcctttgatgctgtccctcccagcattattgaacatcattcttctcatcttcctggtcatgttcatctatgccgtatttggaatgtataattttgcctatgttaaaaaagaagctggaattaatgatgtgtctaattttgaaacctttggcaacagtatgctctgtctttttcaagttgcaatatttgctggttgggatgggatgcttgatgcaattttcaacagtaaatggtctgactgtgatcctgataaaattaaccctgggactcaagttagaggagattgtgggaacccctctgttgggattttttattttgtcagttatatcctcatatcatggctgatcattgtaaatatgtacattgttgttgtcatggagtttttaaatattgcttctaagaagaaaaacaagaccttgagtgaagatgattttaggaaattctttcaggtatggaaaaggtttgatcctgataggacccagtacatagactctagcaagctttcagattttgcagctgctcttgatcctcctcttttcatggcaaaaccaaacaagggccagctcattgctttggacctccccatggctgttggggacagaattcattgcctcgatatcttacttgcttttacaaagagagttatgggtcaagatgtgaggatggagaaagttgtttcagaaatagaatcagggtttttgttagccaacccttttaagatcacatgtgagccaattacgactactttgaaacgaaaacaagaggcagtttcagcaaccatcattcaacgtgcttataaaaattaccgcttgaggcgaaatgacaaaaatacatcagatattcatatgatagatggtgacagagatgttcatgctactaaagaaggtgcctattttgacaaagctaaggaaaagtcacctattcaaagccagatctaa(配列番号11)
H.s.SCN8A
atggcagcgcggctgcttgcaccaccaggccctgatagtttcaagcctttcacccctgagtcactggcaaacattgagaggcgcattgctgagagcaagctcaagaaaccaccaaaggccgatggcagtcatcgggaggacgatgaggacagcaagcccaagccaaacagcgacctggaagcagggaagagtttgcctttcatctacggggacatcccccaaggcctggttgcagttcccctggaggactttgacccatactatttgacgcagaaaacctttgtagtattaaacagagggaaaactctcttcagatttagtgccacgcctgccttgtacattttaagtccttttaacctgataagaagaatagctattaaaattttgatacattcagtatttagcatgatcattatgtgcactattttgaccaactgtgtattcatgacttttagtaaccctcctgactggtcgaagaatgtggagtacacgttcacagggatttatacatttgaatcactagtgaaaatcattgcaagaggtttctgcatagatggctttacctttttacgggacccatggaactggttagatttcagtgtcatcatgatggcgtatataacagagtttgtaaacctaggcaatgtttcagctctacgcactttcagggtactgagggctttgaaaactatttcggtaatcccaggcctgaagacaattgtgggtgccctgattcagtctgtgaagaaactgtcagatgtgatgatcctgacagtgttctgcctgagtgtttttgccttgatcggactgcagctgttcatggggaaccttcgaaacaagtgtgttgtgtggcccataaacttcaacgagagctatcttgaaaatggcaccaaaggctttgattgggaagagtatatcaacaataaaacaaatttctacacagttcctggcatgctggaacctttactctgtgggaacagttctgatgctgggcaatgcccagagggataccagtgtatgaaagcaggaaggaaccccaactatggttacacaagttttgacacttttagctgggccttcttggcattatttcgccttatgacccaggactattgggaaaacttgtatcaattgactttacgagcagccgggaaaacatacatgatcttcttcgtcttggtcatctttgtgggttctttctatctggtgaacttgatcttggctgtggtggccatggcttatgaagaacagaatcaggcaacactggaggaggcagaacaaaaagaggctgaatttaaagcaatgttggagcaacttaagaagcaacaggaagaggcacaggctgctgcgatggccacttcagcaggaactgtctcagaagatgccatagaggaagaaggtgaagaaggagggggctcccctcggagctcttctgaaatctctaaactcagctcaaagagtgcaaaggaaagacgtaacaggagaaagaagaggaagcaaaaggaactctctgaaggagaggagaaaggggatcccgagaaggtgtttaagtcagagtcagaagatggcatgagaaggaaggcctttcggctgccagacaacagaatagggaggaaattttccatcatgaatcagtcactgctcagcatcccaggctcgcccttcctctcccgccacaacagcaagagcagcatcttcagtttcaggggacctgggcggttccgagacccgggctccgagaatgagttcgcggatgacgagcacagcacggtggaggagagcgagggccgccgggactccctcttcatccccatccgggcccgcgagcgccggagcagctacagcggctacagcggctacagccagggcagccgctcctcgcgcatcttccccagcctgcggcgcagcgtgaagcgcaacagcacggtggactgcaacggcgtggtgtccctcatcggcggccccggctcccacatcggcgggcgtctcctgccagaggctacaactgaggtggaaattaagaagaaaggccctggatctcttttagtttccatggaccaattagcctcctacgggcggaaggacagaatcaacagtataatgagtgttgttacaaatacactagtagaagaactggaagagtctcagagaaagtgcccgccatgctggtataaatttgccaacactttcctcatctgggagtgccacccctactggataaaactgaaagagattgtgaacttgatagttatggacccttttgtggatttagccatcaccatctgcatcgtcctgaatacactgtttatggcaatggagcaccatcctatgacaccacaatttgaacatgtcttggctgtaggaaatctggttttcactggaattttcacagcggaaatgttcctgaagctcatagccatggatccctactattatttccaagaaggttggaacatttttgacggatttattgtctccctcagtttaatggaactgagtctagcagacgtggaggggctttcagtgctgcgatctttccgattgctccgagtcttcaaattggccaaatcctggcccaccctgaacatgctaatcaagattattggaaattcagtgggtgccctgggcaacctgacactggtgctggccattattgtcttcatctttgccgtggtggggatgcaactctttggaaaaagctacaaagagtgtgtctgcaagatcaaccaggactgtgaactccctcgctggcatatgcatgactttttccattccttcctcattgtctttcgagtgttgtgcggggagtggattgagaccatgtgggactgcatggaagtggcaggccaggccatgtgcctcattgtctttatgatggtcatggtgattggcaacttggtggtgctgaacctgtttctggccttgctcctgagctccttcagtgcagacaacctggctgccacagatgacgatggggaaatgaacaacctccagatctcagtgatccgtatcaagaagggtgtggcctggaccaaactaaaggtgcacgccttcatgcaggcccactttaagcagcgtgaggctgatgaggtgaagcctctggatgagttgtatgaaaagaaggccaactgtatcgccaatcacaccggtgcagacatccaccggaatggtgacttccagaagaatggcaatggcacaaccagcggcattggcagcagcgtggagaagtacatcattgatgaggaccacatgtccttcatcaacaaccccaacttgactgtacgggtacccattgctgtgggcgagtctgactttgagaacctcaacacagaggatgttagcagcgagtcggatcctgaaggcagcaaagataaactagatgacaccagctcctctgaaggaagcaccattgatatcaaaccagaagtagaagaggtccctgtggaacagcctgaggaatacttggatccagatgcctgcttcacagaaggttgtgtccagcggttcaagtgctgccaggtcaacatcgaggaagggctaggcaagtcttggtggatcctgcggaaaacctgcttcctcatcgtggagcacaactggtttgagaccttcatcatcttcatgattctgctgagcagtggcgccctggccttcgaggacatctacattgagcagagaaagaccatccgcaccatcctggaatatgctgacaaagtcttcacctatatcttcatcctggagatgttgctcaagtggacagcctatggcttcgtcaagttcttcaccaatgcctggtgttggctggacttcctcattgtggctgtctctttagtcagccttatagctaatgccctgggctactcggaactaggtgccataaagtcccttaggaccctaagagctttgagacccttaagagccttatcacgatttgaagggatgagggtggtggtgaatgccttggtgggcgccatcccctccatcatgaatgtgctgctggtgtgtctcatcttctggctgattttcagcatcatgggagttaacttgtttgcgggaaagtaccactactgctttaatgagacttctgaaatccgatttgaaattgaagatgtcaacaataaaactgaatgtgaaaagcttatggaggggaacaatacagagatcagatggaagaacgtgaagatcaactttgacaatgttggggcaggatacctggcccttcttcaagtagcaaccttcaaaggctggatggacatcatgtatgcagctgtagattcccggaagcctgatgagcagcctaagtatgaggacaatatctacatgtacatctattttgtcatcttcatcatcttcggctccttcttcaccctgaacctgttcattggtgtcatcattgataacttcaatcaacaaaagaaaaagttcggaggtcaggacatcttcatgaccgaagaacagaagaagtactacaatgccatgaaaaagctgggctcaaagaagccacagaaacctattccccgccccttgaacaaaatccaaggaatcgtctttgattttgtcactcagcaagcctttgacattgttatcatgatgctcatctgccttaacatggtgacaatgatggtggagacagacactcaaagcaagcagatggagaacatcctctactggattaacctggtgtttgttatcttcttcacctgtgagtgtgtgctcaaaatgtttgcgttgaggcactactacttcaccattggctggaacatcttcgacttcgtggtagtcatcctctccattgtgggaatgttcctggcagatataattgagaaatactttgtttccccaaccctattccgagtcatccgattggcccgtattgggcgcatcttgcgtctgatcaaaggcgccaaagggattcgtaccctgctctttgccttaatgatgtccttgcctgccctgttcaacatcggccttctgctcttcctggtcatgttcatcttctccatttttgggatgtccaattttgcatatgtgaagcacgaggctggtatcgatgacatgttcaactttgagacatttggcaacagcatgatctgcctgtttcaaatcacaacctcagctggttgg
gatggcctgctgctgcccatcctaaaccgcccccctgactgcagcctagataaggaacacccagggagtggctttaagggagattgtgggaacccctcagtgggcatcttcttctttgtaagctacatcatcatctctttcctaattgtcgtgaacatgtacattgccatcatcctggagaacttcagtgtagccacagaggaaagtgcagaccctctgagtgaggatgactttgagaccttctatgagatctgggagaagttcgaccccgatgccacccagttcattgagtactgtaagctggcagactttgcagatgccttggagcatcctctccgagtgcccaagcccaataccattgagctcatcgctatggatctgccaatggtgagcggggatcgcatccactgcttggacatcctttttgccttcaccaagcgggtcctgggagatagcggggagttggacatcctgcggcagcagatggaagagcggttcgtggcatccaatccttccaaagtgtcttacgagccaatcacaaccacactgcgtcgcaagcaggaggaggtatctgcagtggtcctgcagcgtgcctaccggggacatttggcaaggcggggcttcatctgcaaaaagacaacttctaataagctggagaatggaggcacacaccgggagaaaaaagagagcaccccatctacagcctccctcccgtcctatgacagtgtaactaaacctgaaaaggagaaacagcagcgggcagaggaaggaagaagggaaagagccaaaagacaaaaagaggtcagagaatccaagtgttag(配列番号12)
H.s.SCN9A
atggcaatgttgcctcccccaggacctcagagctttgtccatttcacaaaacagtctcttgccctcattgaacaacgcattgctgaaagaaaatcaaaggaacccaaagaagaaaagaaagatgatgatgaagaagccccaaagccaagcagtgacttggaagctggcaaacaactgcccttcatctatggggacattcctcccggcatggtgtcagagcccctggaggacttggacccctactatgcagacaaaaagactttcatagtattgaacaaagggaaaacaatcttccgtttcaatgccacacctgctttatatatgctttctcctttcagtcctctaagaagaatatctattaagattttagtacactccttattcagcatgctcatcatgtgcactattctgacaaactgcatatttatgaccatgaataacccgccggactggaccaaaaatgtcgagtacacttttactggaatatatacttttgaatcacttgtaaaaatccttgcaagaggcttctgtgtaggagaattcacttttcttcgtgacccgtggaactggctggattttgtcgtcattgtttttgcgtatttaacagaatttgtaaacctaggcaatgtttcagctcttcgaactttcagagtattgagagctttgaaaactatttctgtaatcccaggcctgaagacaattgtaggggctttgatccagtcagtgaagaagctttctgatgtcatgatcctgactgtgttctgtctgagtgtgtttgcactaattggactacagctgttcatgggaaacctgaagcataaatgttttcgaaattcacttgaaaataatgaaacattagaaagcataatgaataccctagagagtgaagaagactttagaaaatatttttattacttggaaggatccaaagatgctctcctttgtggtttcagcacagattcaggtcagtgtccagaggggtacacctgtgtgaaaattggcagaaaccctgattatggctacacgagctttgacactttcagctgggccttcttagccttgtttaggctaatgacccaagattactgggaaaacctttaccaacagacgctgcgtgctgctggcaaaacctacatgatcttctttgtcgtagtgattttcctgggctccttttatctaataaacttgatcctggctgtggttgccatggcatatgaagaacagaaccaggcaaacattgaagaagctaaacagaaagaattagaatttcaacagatgttagaccgtcttaaaaaagagcaagaagaagctgaggcaattgcagcggcagcggctgaatatacaagtattaggagaagcagaattatgggcctctcagagagttcttctgaaacatccaaactgagctctaaaagtgctaaagaaagaagaaacagaagaaagaaaaagaatcaaaagaagctctccagtggagaggaaaagggagatgctgagaaattgtcgaaatcagaatcagaggacagcatcagaagaaaaagtttccaccttggtgtcgaagggcataggcgagcacatgaaaagaggttgtctacccccaatcagtcaccactcagcattcgtggctccttgttttctgcaaggcgaagcagcagaacaagtctttttagtttcaaaggcagaggaagagatataggatctgagactgaatttgccgatgatgagcacagcatttttggagacaatgagagcagaaggggctcactgtttgtgccccacagaccccaggagcgacgcagcagtaacatcagccaagccagtaggtccccaccaatgctgccggtgaacgggaaaatgcacagtgctgtggactgcaacggtgtggtctccctggttgatggacgctcagccctcatgctccccaatggacagcttctgccagagggcacgaccaatcaaatacacaagaaaaggcgttgtagttcctatctcctttcagaggatatgctgaatgatcccaacctcagacagagagcaatgagtagagcaagcatattaacaaacactgtggaagaacttgaagagtccagacaaaaatgtccaccttggtggtacagatttgcacacaaattcttgatctggaattgctctccatattggataaaattcaaaaagtgtatctattttattgtaatggatccttttgtagatcttgcaattaccatttgcatagttttaaacacattatttatggctatggaacaccacccaatgactgaggaattcaaaaatgtacttgctataggaaatttggtctttactggaatctttgcagctgaaatggtattaaaactgattgccatggatccatatgagtatttccaagtaggctggaatatttttgacagccttattgtgactttaagtttagtggagctctttctagcagatgtggaaggattgtcagttctgcgatcattcagactgctccgagtcttcaagttggcaaaatcctggccaacattgaacatgctgattaagatcattggtaactcagtaggggctctaggtaacctcaccttagtgttggccatcatcgtcttcatttttgctgtggtcggcatgcagctctttggtaagagctacaaagaatgtgtctgcaagatcaatgatgactgtacgctcccacggtggcacatgaacgacttcttccactccttcctgattgtgttccgcgtgctgtgtggagagtggatagagaccatgtgggactgtatggaggtcgctggtcaagctatgtgccttattgtttacatgatggtcatggtcattggaaacctggtggtcctaaacctatttctggccttattattgagctcatttagttcagacaatcttacagcaattgaagaagaccctgatgcaaacaacctccagattgcagtgactagaattaaaaagggaataaattatgtgaaacaaaccttacgtgaatttattctaaaagcattttccaaaaagccaaagatttccagggagataagacaagcagaagatctgaatactaagaaggaaaactatatttctaaccatacacttgctgaaatgagcaaaggtcacaatttcctcaaggaaaaagataaaatcagtggttttggaagcagcgtggacaaacacttgatggaagacagtgatggtcaatcatttattcacaatcccagcctcacagtgacagtgccaattgcacctggggaatccgatttggaaaatatgaatgctgaggaacttagcagtgattcggatagtgaatacagcaaagtgagattaaaccggtcaagctcctcagagtgcagcacagttgataaccctttgcctggagaaggagaagaagcagaggctgaacctatgaattccgatgagccagaggcctgtttcacagatggttgtgtacggaggttctcatgctgccaagttaacatagagtcagggaaaggaaaaatctggtggaacatcaggaaaacctgctacaagattgttgaacacagttggtttgaaagcttcattgtcctcatgatcctgctcagcagtggtgccctggcttttgaagatatttatattgaaaggaaaaagaccattaagattatcctggagtatgcagacaagatcttcacttacatcttcattctggaaatgcttctaaaatggatagcatatggttataaaacatatttcaccaatgcctggtgttggctggatttcctaattgttgatgtttctttggttactttagtggcaaacactcttggctactcagatcttggccccattaaatcccttcggacactgagagctttaagacctctaagagccttatctagatttgaaggaatgagggtcgttgtgaatgcactcataggagcaattccttccatcatgaatgtgctacttgtgtgtcttatattctggctgatattcagcatcatgggagtaaatttgtttgctggcaagttctatgagtgtattaacaccacagatgggtcacggtttcctgcaagtcaagttccaaatcgttccgaatgttttgcccttatgaatgttagtcaaaatgtgcgatggaaaaacctgaaagtgaactttgataatgtcggacttggttacctatctctgcttcaagttgcaacttttaagggatggacgattattatgtatgcagcagtggattctgttaatgtagacaagcagcccaaatatgaatatagcctctacatgtatatttattttgtcgtctttatcatctttgggtcattcttcactttgaacttgttcattggtgtcatcatagataatttcaaccaacagaaaaagaagcttggaggtcaagacatctttatgacagaagaacagaagaaatactataatgcaatgaaaaagctggggtccaagaagccacaaaagccaattcctcgaccagggaacaaaatccaaggatgtatatttgacctagtgacaaatcaagcctttgatattagtatcatggttcttatctgtctcaacatggtaaccatgatggtagaaaaggagggtcaaagtcaacatatgactgaagttttatattggataaatgtggtttttataatccttttcactggagaatgtgtgctaaaactgatctccctcagacactactacttcactgtaggatggaatatttttgattttgtggttgtgattatctccattgtaggtatgtttctagctgatttgattgaaacgtattttgtgtcccctaccctgttccgagtgatccgtcttgccaggattggccgaatcctacgtctagtcaaaggagcaaaggggatccgcacgctgctctttgctttgatgatgtcccttcctgcgttgtttaacatcggcctcctgctcttcctggtcatgttcatctacgccatctttggaatgtccaactttgcctatgttaaaaaggaagatggaattaatgacatgttcaattttgagacctttggcaacagtatgatttgcctgttccaaattacaacctctgctggctgggatggattgctagcacctattcttaacagtaagccacccgactgtgacccaaaa
aaagttcatcctggaagttcagttgaaggagactgtggtaacccatctgttggaatattctactttgttagttatatcatcatatccttcctggttgtggtgaacatgtacattgcagtcatactggagaattttagtgttgccactgaagaaagtactgaacctctgagtgaggatgactttgagatgttctatgaggtttgggagaagtttgatcccgatgcgacccagtttatagagttctctaaactctctgattttgcagctgccctggatcctcctcttctcatagcaaaacccaacaaagtccagctcattgccatggatctgcccatggttagtggtgaccggatccattgtcttgacatcttatttgcttttacaaagcgtgttttgggtgagagtggggagatggattctcttcgttcacagatggaagaaaggttcatgtctgcaaatccttccaaagtgtcctatgaacccatcacaaccacactaaaacggaaacaagaggatgtgtctgctactgtcattcagcgtgcttatagacgttaccgcttaaggcaaaatgtcaaaaatatatcaagtatatacataaaagatggagacagagatgatgatttactcaataaaaaagatatggcttttgataatgttaatgagaactcaagtccagaaaaaacagatgccacttcatccaccacctctccaccttcatatgatagtgtaacaaagccagacaaagagaaatatgaacaagacagaacagaaaaggaagacaaagggaaagacagcaaggaaagcaaaaaatag(配列番号13)
H.s.SCN10A
atggaattccccattggatccctcgaaactaacaacttccgtcgctttactccggagtcactggtggagatagagaagcaaattgctgccaagcagggaacaaagaaagccagagagaagcatagggagcagaaggaccaagaagagaagcctcggccccagctggacttgaaagcctgcaaccagctgcccaagttctatggtgagctcccagcagaactgatcggggagcccctggaggatctagatccgttctacagcacacaccggacatttatggtgctgaacaaagggaggaccatttcccggtttagtgccactcgggccctgtggctattcagtcctttcaacctgatcagaagaacggccatcaaagtgtctgtccactcgtggttcagtttatttattacggtcactattttggttaattgtgtgtgcatgacccgaactgaccttccagagaaaattgaatatgtcttcactgtcatttacacctttgaagccttgataaagatactggcaagaggattttgtctaaatgagttcacgtacctgagagatccttggaactggctggattttagcgtcattaccctggcatatgttggcacagcaatagatctccgtgggatctcaggcctgcggacattcagagttcttagagcattaaaaacagtttctgtgatcccaggcctgaaggtcattgtgggggccctgattcactcagtgaagaaactggctgatgtgaccatcctcaccatcttctgcctaagtgtttttgccttggtggggctgcaactcttcaagggcaacctcaaaaataaatgtgtcaagaatgacatggctgtcaatgagacaaccaactactcatctcacagaaaaccagatatctacataaataagcgaggcacttctgaccccttactgtgtggcaatggatctgactcaggccactgccctgatggttatatctgccttaaaacttctgacaacccggattttaactacaccagctttgattcctttgcttgggctttcctctcactgttccgcctcatgacacaggattcctgggaacgcctctaccagcagaccctgaggacttctgggaaaatctatatgatcttttttgtgctcgtaatcttcctgggatctttctacctggtcaacttgatcttggctgtagtcaccatggcgtatgaggagcagaaccaggcaaccactgatgaaattgaagcaaaggagaagaagttccaggaggccctcgagatgctccggaaggagcaggaggtgctagcagcactagggattgacacaacctctctccactcccacaatggatcacctttaacctccaaaaatgccagtgagagaaggcatagaataaagccaagagtgtcagagggctccacagaagacaacaaatcaccccgctctgatccttacaaccagcgcaggatgtcttttctaggcctcgcctctggaaaacgccgggctagtcatggcagtgtgttccatttccggtcccctggccgagatatctcactccctgagggagtcacagatgatggagtctttcctggagaccacgaaagccatcggggctctctgctgctgggtgggggtgctggccagcaaggccccctccctagaagccctcttcctcaacccagcaaccctgactccaggcatggagaagatgaacaccaaccgccgcccactagtgagcttgcccctggagctgtcgatgtctcggcattcgatgcaggacaaaagaagactttcttgtcagcagaatacttagatgaacctttccgggcccaaagggcaatgagtgttgtcagtatcataacctccgtccttgaggaactcgaggagtctgaacagaagtgcccaccctgcttgaccagcttgtctcagaagtatctgatctgggattgctgccccatgtgggtgaagctcaagacaattctctttgggcttgtgacggatccctttgcagagctcaccatcaccttgtgcatcgtggtgaacaccatcttcatggccatggagcaccatggcatgagccctaccttcgaagccatgctccagataggcaacatcgtctttaccatattttttactgctgaaatggtcttcaaaatcattgccttcgacccatactattatttccagaagaagtggaatatctttgactgcatcatcgtcactgtgagtctgctagagctgggcgtggccaagaagggaagcctgtctgtgctgcggagcttccgcttgctgcgcgtattcaagctggccaaatcctggcccaccttaaacacactcatcaagatcatcggaaactcagtgggggcactggggaacctcaccatcatcctggccatcattgtctttgtctttgctctggttggcaagcagctcctaggggaaaactaccgtaacaaccgaaaaaatatctccgcgccccatgaagactggccccgctggcacatgcacgacttcttccactctttcctcattgtcttccgtatcctctgtggagagtggattgagaacatgtgggcctgcatggaagttggccaaaaatccatatgcctcatccttttcttgacggtgatggtgctagggaacctggtggtgcttaacctgttcatcgccctgctattgaactctttcagtgctgacaacctcacagccccggaggacgatggggaggtgaacaacctgcaggtggccctggcacggatccaggtctttggccatcgtaccaaacaggctctttgcagcttcttcagcaggtcctgcccattcccccagcccaaggcagagcctgagctggtggtgaaactcccactctccagctccaaggctgagaaccacattgctgccaacactgccagggggagctctggagggctccaagctcccagaggccccagggatgagcacagtgacttcatcgctaatccgactgtgtgggtctctgtgcccattgctgagggtgaatctgatcttgatgacttggaggatgatggtggggaagatgctcagagcttccagcaggaagtgatccccaaaggacagcaggagcagctgcagcaagtcgagaggtgtggggaccacctgacacccaggagcccaggcactggaacatcttctgaggacctggctccatccctgggtgagacgtggaaagatgagtctgttcctcaggtccctgctgagggagtggacgacacaagctcctctgagggcagcacggtggactgcctagatcctgaggaaatcctgaggaagatccctgagctggcagatgacctggaagaaccagatgactgcttcacagaaggatgcattcgccactgtccctgctgcaaactggataccaccaagagtccatgggatgtgggctggcaggtgcgcaagacttgctaccgtatcgtggagcacagctggtttgagagcttcatcatcttcatgatcctgctcagcagtggatctctggcctttgaagactattacctggaccagaagcccacggtgaaagctttgctggagtacactgacagggtcttcacctttatctttgtgttcgagatgctgcttaagtgggtggcctatggcttcaaaaagtacttcaccaatgcctggtgctggctggacttcctcattgtgaatatctcactgataagtctcacagcgaagattctggaatattctgaagtggctcccatcaaagcccttcgaacccttcgcgctctgcggccactgcgggctctttctcgatttgaaggcatgcgggtggtggtggatgccctggtgggcgccatcccatccatcatgaatgtcctcctcgtctgcctcatcttctggctcatcttcagcatcatgggtgtgaacctcttcgcagggaagttttggaggtgcatcaactataccgatggagagttttcccttgtacctttgtcgattgtgaataacaagtctgactgcaagattcaaaactccactggcagcttcttctgggtcaatgtgaaagtcaactttgataatgttgcaatgggttaccttgcacttctgcaggtggcaacctttaaaggctggatggacattatgtatgcagctgttgattcccgggaggtcaacatgcaacccaagtgggaggacaacgtgtacatgtatttgtactttgtcatcttcatcatttttggaggcttcttcacactgaatctctttgttggggtcataattgacaacttcaatcaacagaaaaaaaagttagggggccaggacatcttcatgacagaggagcagaagaaatactacaatgccatgaagaagttgggctccaagaagccccagaagcccatcccacggcccctgaacaagttccagggttttgtctttgacatcgtgaccagacaagcttttgacatcaccatcatggtcctcatctgcctcaacatgatcaccatgatggtggagactgatgaccaaagtgaagaaaagacgaaaattctgggcaaaatcaaccagttctttgtggccgtcttcacaggcgaatgtgtcatgaagatgttcgctttgaggcagtactacttcacaaatggctggaatgtgtttgacttcattgtggtggttctctccattgcgagcctgattttttctgcaattcttaagtcacttcaaagttacttctccccaacgctcttcagagtcatccgcctggcccgaattggccgcatcctcagactgatccgagcggccaaggggatccgcacactgctctttgccctcatgatgtccctgcctgccctcttcaacatcgggctgttgctattccttgtcatgttcatctactctatcttcggtatgtccagctttccccatgtgaggtgggaggctggcatcgacgacatgttcaacttccagaccttcgccaacagcatgctgtgcctcttccagattaccacgtcggccggctgggatggcctcctcagccccatcctcaacacagggcccccctactgtgaccccaatctgcccaacagcaatggcaccagaggggactgtgggagcccagccgtaggcatcatcttcttcaccacctacatcatc
atctccttcctcatcatggtcaacatgtacattgcagtgattctggagaacttcaatgtggccacggaggagagcactgagcccctgagtgaggacgactttgacatgttctatgagacctgggagaagtttgacccagaggccactcagtttattaccttttctgctctctcggactttgcagacactctctctggtcccctgagaatcccaaaacccaatcgaaatatactgatccagatggacctgcctttggtccctggagataagatccactgcttggacatcctttttgctttcaccaagaatgtcctaggagaatccggggagttggattctctgaaggcaaatatggaggagaagtttatggcaactaatctttcaaaatcatcctatgaaccaatagcaaccactctccgatggaagcaagaagacatttcagccactgtcattcaaaaggcctatcggagctatgtgctgcaccgctccatggcactctctaacaccccatgtgtgcccagagctgaggaggaggctgcatcactcccagatgaaggttttgttgcattcacagcaaatgaaaattgtgtactcccagacaaatctgaaactgcttctgccacatcattcccaccgtcctatgagagtgtcactagaggccttagtgatagagtcaacatgaggacatctagctcaatacaaaatgaagatgaagccaccagtatggagctgattgcccctgggccctag(配列番号14)
H.s.SCN11A
atggatgacagatgctacccagtaatctttccagatgagcggaatttccgccccttcacttccgactctctggctgcaattgagaagcggattgccatccaaaaggagaaaaagaagtctaaagaccagacaggagaagtaccccagcctcggcctcagcttgacctaaaggcctccaggaagttgcccaagctctatggcgacattcctcgtgagctcataggaaagcctctggaagacttggacccattctaccgaaatcataagacatttatggtgttaaacagaaagaggacaatctaccgcttcagtgccaagcatgccttgttcatttttgggcctttcaattcaatcagaagtttagccattagagtctcagtccattcattgttcagcatgttcattatcggcaccgttatcatcaactgcgtgttcatggctacagggcctgctaaaaacagcaacagtaacaatactgacattgcagagtgtgtcttcactgggatttatatttttgaagctttgattaaaatattggcaagaggtttcattctggatgagttttctttccttcgagatccatggaactggctggactccattgtcattggaatagcgattgtgtcatatattccaggaatcaccatcaaactattgcccctgcgtaccttccgtgtgttcagagctttgaaagcaatttcagtagtttcacgtctgaaggtcatcgtgggggccttgctacgctctgtgaagaagctggtcaacgtgattatcctcaccttcttttgcctcagcatctttgccctggtaggtcagcagctcttcatgggaagtctgaacctgaaatgcatctcgagggactgtaaaaatatcagtaacccggaagcttatgaccattgctttgaaaagaaagaaaattcacctgaattcaaaatgtgtggcatctggatgggtaacagtgcctgttccatacaatatgaatgtaagcacaccaaaattaatcctgactataattatacgaattttgacaactttggctggtcttttcttgccatgttccggctgatgacccaagattcctgggagaagctttatcaacagaccctgcgtactactgggctctactcagtcttcttcttcattgtggtcattttcctgggctccttctacctgattaacttaaccctggctgttgttaccatggcatatgaggagcagaacaagaatgtagctgcagagatagaggccaaggaaaagatgtttcaggaagcccagcagctgttaaaggaggaaaaggaggctctggttgccatgggaattgacagaagttcacttacttcccttgaaacatcatattttaccccaaaaaagagaaagctctttggtaataagaaaaggaagtccttctttttgagagagtctgggaaagaccagcctcctgggtcagattctgatgaagattgccaaaaaaagccacagctcctagagcaaaccaaacgactgtcccagaatctatcactggaccactttgatgagcatggagatcctctccaaaggcagagagcactgagtgctgtcagcatcctcaccatcaccatgaaggaacaagaaaaatcacaagagccttgtctcccttgtggagaaaacctggcatccaagtacctcgtgtggaactgttgcccccagtggctgtgcgttaagaaggtcctgagaactgtgatgactgacccgtttactgagctggccatcaccatctgcatcatcatcaacactgtcttcttggccatggagcatcacaagatggaggccagttttgagaagatgttgaatatagggaatttggttttcactagcatttttatagcagaaatgtgcctaaaaatcattgcgctcgatccctaccactactttcgccgaggctggaacatttttgacagcattgttgctcttctgagttttgcagatgtaatgaactgtgtacttcaaaagagaagctggccattcttgcgttccttcagagtgctcagggtcttcaagttagccaaatcctggccaactttgaacacactaattaagataatcggcaactctgtcggagcccttggaagcctgactgtggtcctggtcattgtgatctttattttctcagtagttggcatgcagctttttggccgtagcttcaattcccaaaagagtccaaaactctgtaacccgacaggcccgacagtctcatgtttacggcactggcacatgggggatttctggcactccttcctagtggtattccgcatcctctgcggggaatggatcgaaaatatgtgggaatgtatgcaagaagcgaatgcatcatcatcattgtgtgttattgtcttcatattgatcacggtgataggaaaacttgtggtgctcaacctcttcattgccttactgctcaattcctttagcaatgaggaaagaaatggaaacttagaaggagaggccaggaaaactaaagtccagttagcactggatcgattccgccgggctttttgttttgtgagacacactcttgagcatttctgtcacaagtggtgcaggaagcaaaacttaccacagcaaaaagaggtggcaggaggctgtgctgcacaaagcaaagacatcattcccctggtcatggagatgaaaaggggctcagagacccaggaggagcttggtatactaacctctgtaccaaagaccctgggcgtcaggcatgattggacttggttggcaccacttgcggaggaggaagatgacgttgaattttctggtgaagataatgcacagcgcatcacacaacctgagcctgaacaacaggcctatgagctccatcaggagaacaagaagcccacgagccagagagttcaaagtgtggaaattgacatgttctctgaagatgagcctcatctgaccatacaggatccccgaaagaagtctgatgttaccagtatactatcagaatgtagcaccattgatcttcaggatggctttggatggttacctgagatggttcccaaaaagcaaccagagagatgtttgcccaaaggctttggttgctgctttccatgctgtagcgtggacaagagaaagcctccctgggtcatttggtggaacctgcggaaaacctgctaccaaatagtgaaacacagctggtttgagagctttattatctttgtgattctgctgagcagtggggcactgatatttgaagatgttcaccttgagaaccaacccaaaatccaagaattactaaattgtactgacattatttttacacatatttttatcctggagatggtactaaaatgggtagccttcggatttggaaagtatttcaccagtgcctggtgctgccttgatttcatcattgtgattgtctctgtgaccaccctcattaacttaatggaattgaagtccttccggactctacgagcactgaggcctcttcgtgcgctgtcccagtttgaaggaatgaaggtggtggtcaatgctctcataggtgccatacctgccattctgaatgttttgcttgtctgcctcattttctggctcgtattttgtattctgggagtatacttcttttctggaaaatttgggaaatgcattaatggaacagactcagttataaattataccatcattacaaataaaagtcaatgtgaaagtggcaatttctcttggatcaaccagaaagtcaactttgacaatgtgggaaatgcttacctcgctctgctgcaagtggcaacatttaagggctggatggatattatatatgcagctgttgattccacagagaaagaacaacagccagagtttgagagcaattcactcggttacatttacttcgtagtctttatcatctttggctcattcttcactctgaatctcttcattggcgttatcattgacaacttcaaccaacagcagaaaaagttaggtggccaagacatttttatgacagaagaacagaagaaatactataatgcaatgaaaaaattaggatccaaaaaacctcaaaaacccattccacggcctctgaacaaatgtcaaggtctcgtgttcgacatagtcacaagccagatctttgacatcatcatcataagtctcattatcctaaacatgattagcatgatggctgaatcatacaaccaacccaaagccatgaaatccatccttgaccatctcaactgggtctttgtggtcatctttacgttagaatgtctcatcaaaatctttgctttgaggcaatactacttcaccaatggctggaatttatttgactgtgtggtcgtgcttctttccattgttagtacaatgatttctaccttggaaaatcaggagcacattcctttccctccgacgctcttcagaattgtccgcttggctcggattggccgaatcctgaggcttgtccgggctgcacgaggaatcaggactctcctctttgctctgatgatgtcgcttccttctctgttcaacattggtcttctactctttctgattatgtttatctatgccattctgggtatgaactggttttccaaagtgaatccagagtctggaatcgatgacatattcaacttcaagacttttgccagcagcatgctctgtctcttccagataagcacatcagcaggttgggattccctgctcagccccatgctgcgatcaaaagaatcatgtaactcttcctcagaaaactgccacctccctggcatagccacatcctactttgtcagttacattatcatctcctttctcattgttgtcaacatgtacattgctgtgattttagagaacttcaatacagccactgaagaaagtgaggaccctttgggtgaagatgactttgacatattttatgaagtgtgggaaaagtttgacccagaagcaacacaatttatcaaatattctgccctttctgactttgctgatgccttgcctgagcctttgcgtgtcgcaaagccaaataaatatcaatttctagtaatggacttgcccatggtgagtgaagatcgcctccactgcatggatattcttttcgccttcaccgctagggtactcggtggctctgatggcctagatagtatgaaagcaatgatg
gaagagaagttcatggaagccaatcctctcaagaagttgtatgaacccatagtcaccaccaccaagagaaaggaagaggaaagaggtgctgctattattcaaaaggcctttcgaaagtacatgatgaaggtgaccaagggtgaccaaggtgaccaaaatgacttggaaaacgggcctcattcaccactccagactctttgcaatggagacttgtctagctttggggtggccaagggcaaggtccactgtgactga(配列番号15)
H.s.SCN1B
Atggggaggctgctggccttagtggtcggcgcggcactggtgtcctcagcctgcgggggctgcgtggaggtggactcggagaccgaggccgtgtatgggatgaccttcaaaattctttgcatctcctgcaagcgccgcagcgagaccaacgctgagaccttcaccgagtggaccttccgccagaagggcactgaggagtttgtcaagatcctgcgctatgagaatgaggtgttgcagctggaggaggatgagcgcttcgagggccgcgtggtgtggaatggcagccggggcaccaaagacctgcaggatctgtctatcttcatcaccaatgtcacctacaaccactcgggcgactacgagtgccacgtctaccgcctgctcttcttcgaaaactacgagcacaacaccagcgtcgtcaagaagatccacattgaggtagtggacaaagccaacagagacatggcatccatcgtgtctgagatcatgatgtatgtgctcattgtggtgttgaccatatggctcgtggcagagatgatttactgctacaagaagatcgctgccgccacggagactgctgcacaggagaatgcctcggaatacctggccatcacctctgaaagcaaagagaactgcacgggcgtccaggtggccgaatag(配列番号16)
H.s.SCN2B
Atgcacagagatgcctggctacctcgccctgccttcagcctcacggggctcagtctctttttctctttggtgccaccaggacggagcatggaggtcacagtacctgccaccctcaacgtcctcaatggctctgacgcccgcctgccctgcaccttcaactcctgctacacagtgaaccacaaacagttctccctgaactggacttaccaggagtgcaacaactgctctgaggagatgttcctccagttccgcatgaagatcattaacctgaagctggagcggtttcaagaccgcgtggagttctcagggaaccccagcaagtacgatgtgtcggtgatgctgagaaacgtgcagccggaggatgaggggatttacaactgctacatcatgaacccccctgaccgccaccgtggccatggcaagatccatctgcaggtcctcatggaagagccccctgagcgggactccacggtggccgtgattgtgggtgcctccgtcgggggcttcctggctgtggtcatcttggtgctgatggtggtcaagtgtgtgaggagaaaaaaagagcagaagctgagcacagatgacctgaagaccgaggaggagggcaagacggacggtgaaggcaacccggatgatggcgccaagtag(配列番号17)
H.s.SCN3B
Atgcctgccttcaatagattgtttcccctggcttctctcgtgcttatctactgggtcagtgtctgcttccctgtgtgtgtggaagtgccctcggagacggaggccgtgcagggcaaccccatgaagctgcgctgcatctcctgcatgaagagagaggaggtggaggccaccacggtggtggaatggttctacaggcccgagggcggtaaagatttccttatttacgagtatcggaatggccaccaggaggtggagagcccctttcaggggcgcctgcagtggaatggcagcaaggacctgcaggacgtgtccatcactgtgctcaacgtcactctgaacgactctggcctctacacctgcaatgtgtcccgggagtttgagtttgaggcgcatcggccctttgtgaagacgacgcggctgatccccctaagagtcaccgaggaggctggagaggacttcacctctgtggtctcagaaatcatgatgtacatccttctggtcttcctcaccttgtggctgctcatcgagatgatatattgctacagaaaggtctcaaaagccgaagaggcagcccaagaaaacgcgtctgactaccttgccatcccatctgagaacaaggagaactctgcggtaccagtggaggaatag(配列番号18)
H.s.SCN4B
Atgcccggggctggggacggaggcaaagccccggcgagatggctgggcactgggcttttgggcctcttcctgctccccgtaaccctgtcgctggaggtgtctgtgggaaaggccaccgacatctacgctgtcaatggcacggagatcctgctgccctgcaccttctccagctgctttggcttcgaggacctccacttccggtggacctacaacagcagtgacgcattcaagattctcatagaggggactgtgaagaatgagaagtctgaccccaaggtgacgttgaaagacgatgaccgcatcactctggtaggctctactaaggagaagatgaacaacatttccattgtgctgagggacctggagttcagcgacacgggcaaatacacctgccatgtgaagaaccccaaggagaataatctccagcaccacgccaccatcttcctccaagtcgttgatagactggaagaagtggacaacacagtgacactcatcatcctggctgtcgtgggcggggtcatcgggctcctcatcctcatcctgctgatcaagaaactcatcatcttcatcctgaagaagactcgggagaagaagaaggagtgtctcgtgagctcctcggggaatgacaacacggagaacggcttgcctggctccaaggcagaggagaaaccaccttcaaaagtgtga(配列番号19)
H.s.SCN1A
meqtvlvppgpdsfnfftreslaaierriaeekaknpkpdkkdddengpkpnsdleagknlpfiygdippemvsepledldpyyinkktfivlnkgkaifrfsatsalyiltpfnplrkiaikilvhslfsmlimctiltncvfmtmsnppdwtknveytftgiytfeslikiiargfcledftflrdpwnwldftvitfayvtefvdlgnvsalrtfrvlralktisvipglktivgaliqsvkklsdvmiltvfclsvfaliglqlfmgnlrnkciqwpptnasleehsieknitvnyngtlinetvfefdwksyiqdsryhyflegfldallcgnssdagqcpegymcvkagrnpnygytsfdtfswaflslfrlmtqdfwenlyqltlraagktymiffvlviflgsfylinlilavvamayeeqnqatleeaeqkeaefqqmieqlkkqqeaaqqaatatasehsrepsaagrlsdssseasklssksakerrnrrkkrkqkeqsggeekdedefqksesedsirrkgfrfsiegnrltyekryssphqsllsirgslfsprrnsrtslfsfrgrakdvgsendfaddehstfednesrrdslfvprrhgerrnsnlsqtsrssrmlavfpangkmhstvdcngvvslvggpsvptspvgqllpegtttetemrkrrsssfhvsmdfledpsqrqramsiasiltntveeleesrqkcppcwykfsnifliwdcspywlkvkhvvnlvvmdpfvdlaiticivlntlfmamehypmtdhfnnvltvgnlvftgiftaemflkiiamdpyyyfqegwnifdgfivtlslvelglanveglsvlrsfrllrvfklakswptlnmlikiignsvgalgnltlvlaiivfifavvgmqlfgksykdcvckiasdcqlprwhmndffhsflivfrvlcgewietmwdcmevagqamcltvfmmvmvignlvvlnlflalllssfsadnlaatdddnemnnlqiavdrmhkgvayvkrkiyefiqqsfirkqkildeikplddlnnkkdscmsnhtaeigkdldylkdvngttsgigtgssvekyiidesdymsfinnpsltvtvpiavgesdfenlntedfssesdleeskeklnesssssegstvdigapveeqpvvepeetlepeacftegcvqrfkccqinveegrgkqwwnlrrtcfrivehnwfetfivfmillssgalafediyidqrktiktmleyadkvftyifilemllkwvaygyqtyftnawcwldflivdvslvsltanalgyselgaikslrtlralrplralsrfegmrvvvnallgaipsimnvllvclifwlifsimgvnlfagkfyhcintttgdrfdiedvnnhtdclkliernetarwknvkvnfdnvgfgylsllqvatfkgwmdimyaavdsrnvelqpkyeeslymylyfvifiifgsfftlnlfigviidnfnqqkkkfggqdifmteeqkkyynamkklgskkpqkpiprpgnkfqgmvfdfvtrqvfdisimiliclnmvtmmvetddqseyvttilsrinlvfivlftgecvlklislrhyyftigwnifdfvvvilsivgmflaeliekyfvsptlfrvirlarigrilrlikgakgirtllfalmmslpalfniglllflvmfiyaifgmsnfayvkrevgiddmfnfetfgnsmiclfqittsagwdgllapilnskppdcdpnkvnpgssvkgdcgnpsvgifffvsyiiisflvvvnmyiavilenfsvateesaeplseddfemfyevwekfdpdatqfmefeklsqfaaalepplnlpqpnklqliamdlpmvsgdrihcldilfaftkrvlgesgemdalriqmeerfmasnpskvsyqpitttlkrkqeevsaviiqrayrrhllkrtvkqasftynknkikgganllikedmiidrinensitektdltmstaacppsydrvtkpivekheqegkdekakgk(配列番号20)
H.s.SCN2A
maqsvlvppgpdsfrfftreslaaieqriaeekakrpkqerkdeddengpkpnsdleagkslpfiygdippemvsvpledldpyyinkktfivlnkgkaisrfsatpalyiltpfnpirklaikilvhslfnmlimctiltncvfmtmsnppdwtknveytftgiytfeslikilargfcledftflrdpwnwldftvitfayvtefvdlgnvsalrtfrvlralktisvipglktivgaliqsvkklsdvmiltvfclsvfaliglqlfmgnlrnkclqwppdnssfeinitsffnnsldgngttfnrtvsifnwdeyiedkshfyflegqndallcgnssdagqcpegyicvkagrnpnygytsfdtfswaflslfrlmtqdfwenlyqltlraagktymiffvlviflgsfylinlilavvamayeeqnqatleeaeqkeaefqqmleqlkkqqeeaqaaaaaasaesrdfsgaggigvfsesssvasklssksekelknrrkkkkqkeqsgeeekndrvrksesedsirrkgfrfslegsrltyekrfssphqsllsirgslfsprrnsraslfsfrgrakdigsendfaddehstfedndsrrdslfvphrhgerrhsnvsqasrasrvlpilpmngkmhsavdcngvvslvggpstltsagqllpegttteteirkrrsssyhvsmdlledptsrqramsiasiltntmeeleesrqkcppcwykfanmcliwdcckpwlkvkhlvnlvvmdpfvdlaiticivlntlfmamehypmteqfssvlsvgnlvftgiftaemflkiiamdpyyyfqegwnifdgfivslslmelglanveglsvlrsfrllrvfklakswptlnmlikiignsvgalgnltlvlaiivfifavvgmqlfgksykecvckisndcelprwhmhdffhsflivfrvlcgewietmwdcmevagqtmcltvfmmvmvignlvvlnlflalllssfssdnlaatdddnemnnlqiavgrmqkgidfvkrkirefiqkafvrkqkaldeikpledlnnkkdscisnhttieigkdlnylkdgngttsgigssvekyvvdesdymsfinnpsltvtvpiavgesdfenlnteefssesdmeeskeklnatsssegstvdigapaegeqpevepeeslepeacftedcvrkfkccqisieegkgklwwnlrktcykivehnwfetfivfmillssgalafediyieqrktiktmleyadkvftyifilemllkwvaygfqvyftnawcwldflivdvslvsltanalgyselgaikslrtlralrplralsrfegmrvvvnallgaipsimnvllvclifwlifsimgvnlfagkfyhcinyttgemfdvsvvnnyseckaliesnqtarwknvkvnfdnvglgylsllqvatfkgwmdimyaavdsrnvelqpkyednlymylyfvifiifgsfftlnlfigviidnfnqqkkkfggqdifmteeqkkyynamkklgskkpqkpiprpankfqgmvfdfvtkqvfdisimiliclnmvtmmvetddqsqemtnilywinlvfivlftgecvlklislryyyftigwnifdfvvvilsivgmflaeliekyfvsptlfrvirlarigrilrlikgakgirtllfalmmslpalfniglllflvmfiyaifgmsnfayvkrevgiddmfnfetfgnsmiclfqittsagwdgllapilnsgppdcdpdkdhpgssvkgdcgnpsvgifffvsyiiisflvvvnmyiavilenfsvateesaeplseddfemfyevwekfdpdatqfiefaklsdfadaldpplliakpnkvqliamdlpmvsgdrihcldilfaftkrvlgesgemdalriqmeerfmasnpskvsyepitttlkrkqeevsaiiiqrayrryllkqkvkkvssiykkdkgkecdgtpikedtlidklnenstpektdmtpsttsppsydsvtkpekekfekdksekedkgkdireskk(配列番号21)
H.s.SCN3A
maqallvppgpesfrlftreslaaiekraaeekakkpkkeqdnddenkpkpnsdleagknlpfiygdippemvsepledldpyyinkktfivmnkgkaifrfsatsalyiltplnpvrkiaikilvhslfsmlimctiltncvfmtlsnppdwtknveytftgiytfeslikilargfcledftflrdpwnwldfsvivmayvtefvdlgnvsalrtfrvlralktisvipglktivgaliqsvkklsdvmiltvfclsvfaliglqlfmgnlrnkclqwppsdsafetnttsyfngtmdsngtfvnvtmstfnwkdyigddshfyvldgqkdpllcgngsdagqcpegyicvkagrnpnygytsfdtfswaflslfrlmtqdywenlyqltlraagktymiffvlviflgsfylvnlilavvamayeeqnqatleeaeqkeaefqqmleqlkkqqeeaqavaaasaasrdfsgigglgellessseasklssksakewrnrrkkrrqrehlegnnkgerdsfpksesedsvkrssflfsmdgnrltsdkkfcsphqsllsirgslfsprrnsktsifsfrgrakdvgsendfaddehstfedsesrrdslfvphrhgerrnsnvsqasmssrmvpglpangkmhstvdcngvvslvggpsaltsptgqlppegtttetevrkrrlssyqismemledssgrqravsiasiltntmeeleesrqkcppcwyrfanvfliwdccdawlkvkhlvnlivmdpfvdlaiticivlntlfmamehypmteqfssvltvgnlvftgiftaemvlkiiamdpyyyfqegwnifdgiivslslmelglsnveglsvlrsfrllrvfklakswptlnmlikiignsvgalgnltlvlaiivfifavvgmqlfgksykecvckinddctlprwhmndffhsflivfrvlcgewietmwdcmevagqtmclivfmlvmvignlvvlnlflalllssfssdnlaatdddnemnnlqiavgrmqkgidyvknkmrecfqkaffrkpkvieihegnkidscmsnntgieiskelnylrdgngttsgvgtgssvekyvidendymsfinnpsltvtvpiavgesdfenlnteefsseseleeskeklnatsssegstvdvvlpregeqaetepeedlkpeacftegcikkfpfcqvsteegkgkiwwnlrktcysivehnwfetfivfmillssgalafediyieqrktiktmleyadkvftyifilemllkwvaygfqtyftnawcwldflivdvslvslvanalgyselgaikslrtlralrplralsrfegmrvvvnalvgaipsimnvllvclifwlifsimgvnlfagkfyhcvnmttgnmfdisdvnnlsdcqalgkqarwknvkvnfdnvgagylallqvatfkgwmdimyaavdsrdvklqpvyeenlymylyfvifiifgsfftlnlfigviidnfnqqkkkfggqdifmteeqkkyynamkklgskkpqkpiprpankfqgmvfdfvtrqvfdisimiliclnmvtmmvetddqgkymtlvlsrinlvfivlftgefvlklvslrhyyftigwnifdfvvvilsivgmflaemiekyfvsptlfrvirlarigrilrlikgakgirtllfalmmslpalfniglllflvmfiyaifgmsnfayvkkeagiddmfnfetfgnsmiclfqittsagwdgllapilnsappdcdpdtihpgssvkgdcgnpsvgifffvsyiiisflvvvnmyiavilenfsvateesaeplseddfemfyevwekfdpdatqfiefsklsdfaaaldpplliakpnkvqliamdlpmvsgdrihcldilfaftkrvlgesgemdalriqmedrfmasnpskvsyepitttlkrkqeevsaaiiqrnfrcyllkqrlknissnynkeaikgridlpikqdmiidklngnstpektdgsssttsppsydsvtkpdkekfekdkpekeskgkevrenqk(配列番号22)
H.s.SCN4A
marpslctlvplgpeclrpftreslaaieqraveeearlqrnkqmeieeperkprsdleagknlpmiygdpppevigipledldpyysnkktfivlnkgkaifrfsatpalyllspfsvvrrgaikvlihalfsmfimitiltncvfmtmsdpppwsknveytftgiytfeslikilargfcvddftflrdpwnwldfsvimmayltefvdlgnisalrtfrvlralktitvipglktivgaliqsvkklsdvmiltvfclsvfalvglqlfmgnlrqkcvrwpppfndtnttwysndtwygndtwygnemwygndswyandtwnshaswatndtfdwdayisdegnfyflegsndallcgnssdaghcpegyeciktgrnpnygytsydtfswaflalfrlmtqdywenlfqltlraagktymiffvviiflgsfylinlilavvamayaeqneatlaedkekeeefqqmlekfkkhqeelekakaaqaleggeadgdpahgkdcngsldtsqgekgaprqsssgdsgisdameeleeahqkcppwwykcahkvliwnccapwlkfkniihlivmdpfvdlgiticivlntlfmamehypmtehfdnvltvgnlvftgiftaemvlkliamdpyeyfqqgwnifdsiivtlslvelglanvqglsvlrsfrllrvfklakswptlnmlikiignsvgalgnltlvlaiivfifavvgmqlfgksykecvckialdcnlprwhmhdffhsflivfrilcgewietmwdcmevagqamcltvflmvmvignlvvlnlflalllssfsadslaasdedgemnnlqiaigriklgigfakafllgllhgkilspkdimlslgeadgageageagetapedekkeppeedlkkdnhilnhmgladgppssleldhlnfinnpyltiqvpiaseesdlempteeetdtfsepedskkppqplydgnssvcstadykppeedpeeqaeenpegeqpeecfteacvqrwpclyvdisqgrgkkwwtlrracfkivehnwfetfivfmillssgalafediyieqrrvirtileyadkvftyifimemllkwvaygfkvyftnawcwldflivdvsiislvanwlgyselgpikslrtlralrplralsrfegmrvvvnallgaipsimnvllvclifwlifsimgvnlfagkfyycintttserfdisevnnkseceslmhtgqvrwlnvkvnydnvglgylsllqvatfkgwmdimyaavdsrekeeqpqyevnlymylyfvifiifgsfftlnlfigviidnfnqqkkklggkdifmteeqkkyynamkklgskkpqkpiprpqnkiqgmvydlvtkqafditimiliclnmvtmmvetdnqsqlkvdilyninmifiiiftgecvlkmlalrqyyftvgwnifdfvvvilsivglalsdliqkyfvsptlfrvirlarigrvlrlirgakgirtllfalmmslpalfniglllflvmfiysifgmsnfayvkkesgiddmfnfetfgnsiiclfeittsagwdgllnpilnsgppdcdpnlenpgtsvkgdcgnpsigicffcsyiiisflivvnmyiaiilenfnvateesseplgeddfemfyetwekfdpdatqfiaysrlsdfvdtlqeplriakpnkiklitldlpmvpgdkihcldilfaltkevlgdsgemdalkqtmeekfmaanpskvsyepitttlkrkheevcaikiqrayrrhllqrsmkqasymyrhshdgsgddapekegllantmskmyghengnssspspeekgeagdagptmglmpispsdtawppapppgqtvrpgvkeslv(配列番号23)
H.s.SCN5A
manfllprgtssfrrftreslaaiekrmaekqargsttlqesreglpeeeaprpqldlqaskklpdlygnppqeligepledldpfystqktfivlnkgktifrfsatnalyvlspfhpirraavkilvhslfnmlimctiltncvfmaqhdpppwtkyveytftaiytfeslvkilargfclhaftflrdpwnwldfsviimayttefvdlgnvsalrtfrvlralktisvisglktivgaliqsvkkladvmvltvfclsvfaliglqlfmgnlrhkcvrnftalngtngsveadglvwesldlylsdpenyllkngtsdvllcgnssdagtcpegyrclkagenpdhgytsfdsfawaflalfrlmtqdcwerlyqqtlrsagkiymiffmlviflgsfylvnlilavvamayeeqnqatiaeteekekrfqeamemlkkehealtirgvdtvsrsslemsplapvnsherrskrrkrmssgteecgedrlpksdsedgpramnhlsltrglsrtsmkprssrgsiftfrrrdlgseadfaddenstageseshhtsllvpwplrrtsaqgqpspgtsapghalhgkknstvdcngvvsllgagdpeatspgshllrpvmlehppdtttpseepggpqmltsqapcvdgfeepgarqralsavsvltsaleeleesrhkcppcwnrlaqryliweccplwmsikqgvklvvmdpftdltitmcivlntlfmalehynmtsefeemlqvgnlvftgiftaemtfkiialdpyyyfqqgwnifdsiivilslmelglsrmsnlsvlrsfrllrvfklakswptlntlikiignsvgalgnltlvlaiivfifavvgmqlfgknyselrdsdsgllprwhmmdffhafliifrilcgewietmwdcmevsgqslcllvfllvmvignlvvlnlflalllssfsadnltapdedremnnlqlalariqrglrfvkrttwdfccgllrqrpqkpaalaaqgqlpsciatpysppppetekvpptrketrfeegeqpgqgtpgdpepvcvpiavaesdtddqeedeenslgteeesskqqesqpvsggpeappdsrtwsqvsatasseaeasasqadwrqqwkaepqapgcgetpedscsegstadmtntaelleqipdlgqdvkdpedcftegcvrrcpccavdttqapgkvwwrlrktcyhivehswfetfiifmillssgalafediyleerktikvlleyadkmftyvfvlemllkwvaygfkkyftnawcwldflivdvslvslvantlgfaemgpikslrtlralrplralsrfegmrvvvnalvgaipsimnvllvclifwlifsimgvnlfagkfgrcinqtegdlplnytivnnksqceslnltgelywtkvkvnfdnvgagylallqvatfkgwmdimyaavdsrgyeeqpqweynlymyiyfvifiifgsfftlnlfigviidnfnqqkkklggqdifmteeqkkyynamkklgskkpqkpiprplnkyqgfifdivtkqafdvtimfliclnmvtmmvetddqspekinilakinllfvaiftgecivklaalrhyyftnswnifdfvvvilsivgtvlsdiiqkyffsptlfrvirlarigrilrlirgakgirtllfalmmslpalfniglllflvmfiysifgmanfayvkweagiddmfnfqtfansmlclfqittsagwdgllspilntgppycdptlpnsngsrgdcgspavgilffttyiiisflivvnmyiaiilenfsvateesteplseddfdmfyeiwekfdpeatqfieysvlsdfadalseplriakpnqislinmdlpmvsgdrihcmdilfaftkrvlgesgemdalkiqmeekfmaanpskisyepitttlrrkheevsamviqrafrrhllqrslkhasflfrqqagsglseedaperegliayvmsenfsrplgppssssisstsfppsydsvtratsdnlqvrgsdyshsedladfppspdrdresiv(配列番号24)
H.s.SCN7A(occasionallyreferredtoasSCN6A)
mlaspepkglvpftkesfelikqhiakthnedheeedlkptpdlevgkklpfiygnlsqgmvsepledvdpyyykkkntfivlnknrtifrfnaasilctlspfncirrttikvlvhpffqlfilisvlidcvfmsltnlpkwrpvlentllgiytfeilvklfargvwagsfsflgdpwnwldfsvtvfeviiryspldfiptlqtartlrilkiiplnqglkslvgvlihclkqligviiltlfflsifsligmglfmgnlkhkcfrwpqenenetlhnrtgnpyyiretenfyylegeryallcgnrtdagqcpegyvcvkaginpdqgftnfdsfgwalfalfrlmaqdypevlyhqilyasgkvymiffvvvsflfsfymaslflgilamayeeekqrvgeiskkiepkfqqtgkelqegnetdeaktiqiemkkrspistdtsldvledatlrhkeelekskkicplywykfaktfliwncspcwlklkefvhriimapftdlfliiciilnvcfltlehypmskqtntllnignlvfigiftaemifkiiamhpygyfqvgwnifdsmivfhglielclanvagmallrlfrmlrifklgkywptfqilmwslsnswvalkdlvlllftfiffsaafgmklfgknyeefvchidkdcqlprwhmhdffhsflnvfrilcgewvetlwdcmevagqswcipfylmvilignllvlylflalvssfssckdvtaeenneaknlqlavarikkginyvllkilcktqnvpkdtmdhvnevyvkedisdhtlselsntqdflkdkekssgteknatenesqslipspsvsetvpiasgesdienldnkeiqsksgdggskekikqssssecstvdiaiseeeemfyggerskhlkngcrrgsslgqisgaskkgkiwqnirktcckivennwfkcfiglvtllstgtlafediymdqrktikilleyadmiftyifilemllkwmaygfkayfsngwyrldfvvvivfclsligktreelkplismkflrplrvlsqfermkvvvralikttlptlnvflvclmiwlifsimgvdlfagrfyecidptsgerfpssevmnksrcesllfnesmlwenakmnfdnvgngflsllqvatfngwitimnsaidsvavniqphfevniymycyfinfiifgvflplsmlitviidnfnkhkiklggsnifitvkqrkqyrrlkklmyedsqrpvprplnklqgfifdvvtsqafnvivmvlicfqaiammidtdvqslqmsialywinsifvmlytmecilkliafrcfyftiawnifdfmvvifsitglclpmtvgsylvppslvqlillsriihmlrlgkgpkvfhnlmlplmlslpallniilliflvmfiyavfgmynfayvkkeagindvsnfetfgnsmlclfqvaifagwdgmldaifnskwsdcdpdkinpgtqvrgdcgnpsvgifyfvsyiliswliivnmyivvvmeflniaskkknktlseddfrkffqvwkrfdpdrtqyidssklsdfaaaldpplfmakpnkgqlialdlpmavgdrihcldillaftkrvmgqdvrmekvvseiesgfllanpfkitcepitttlkrkqeavsatiiqrayknyrlrrndkntsdihmidgdrdvhatkegayfdkakekspiqsqi(配列番号25)
H.s.SCN8A
maarllappgpdsfkpftpeslanierriaesklkkppkadgshreddedskpkpnsdleagkslpfiygdipqglvavpledfdpyyltqktfvvlnrgktlfrfsatpalyilspfnlirriaikilihsvfsmiimctiltncvfmtfsnppdwsknveytftgiytfeslvkiiargfcidgftflrdpwnwldfsvimmayitefvnlgnvsalrtfrvlralktisvipglktivgaliqsvkklsdvmiltvfclsvfaliglqlfmgnlrnkcvvwpinfnesylengtkgfdweeyinnktnfytvpgmlepllcgnssdagqcpegyqcmkagrnpnygytsfdtfswaflalfrlmtqdywenlyqltlraagktymiffvlvifvgsfylvnlilavvamayeeqnqatleeaeqkeaefkamleqlkkqqeeaqaaamatsagtvsedaieeegeegggsprssseisklssksakerrnrrkkrkqkelsegeekgdpekvfksesedgmrrkafrlpdnrigrkfsimnqsllsipgspflsrhnskssifsfrgpgrfrdpgsenefaddehstveesegrrdslfipirarerrssysgysgysqgsrssrifpslrrsvkrnstvdcngvvsliggpgshiggrllpeatteveikkkgpgsllvsmdqlasygrkdrinsimsvvtntlveeleesqrkcppcwykfantfliwechpywiklkeivnlivmdpfvdlaiticivlntlfmamehhpmtpqfehvlavgnlvftgiftaemflkliamdpyyyfqegwnifdgfivslslmelsladveglsvlrsfrllrvfklakswptlnmlikiignsvgalgnltlvlaiivfifavvgmqlfgksykecvckinqdcelprwhmhdffhsflivfrvlcgewietmwdcmevagqamclivfmmvmvignlvvlnlflalllssfsadnlaatdddgemnnlqisvirikkgvawtklkvhafmqahfkqreadevkpldelyekkancianhtgadihrngdfqkngngttsgigssvekyiidedhmsfinnpnltvrvpiavgesdfenlntedvssesdpegskdklddtsssegstidikpeveevpveqpeeyldpdacftegcvqrfkccqvnieeglgkswwilrktcflivehnwfetfiifmillssgalafediyieqrktirtileyadkvftyifilemllkwtaygfvkfftnawcwldflivavslvslianalgyselgaikslrtlralrplralsrfegmrvvvnalvgaipsimnvllvclifwlifsimgvnlfagkyhycfnetseirfeiedvnnkteceklmegnnteirwknvkinfdnvgagylallqvatfkgwmdimyaavdsrkpdeqpkyedniymyiyfvifiifgsfftlnlfigviidnfnqqkkkfggqdifmteeqkkyynamkklgskkpqkpiprplnkiqgivfdfvtqqafdivimmliclnmvtmmvetdtqskqmenilywinlvfvifftcecvlkmfalrhyyftigwnifdfvvvilsivgmfladiiekyfvsptlfrvirlarigrilrlikgakgirtllfalmmslpalfniglllflvmfifsifgmsnfayvkheagiddmfnfetfgnsmiclfqittsagwdglllpilnrppdcsldkehpgsgfkgdcgnpsvgifffvsyiiisflivvnmyiaiilenfsvateesadplseddfetfyeiwekfdpdatqfieyckladfadalehplrvpkpntieliamdlpmvsgdrihcldilfaftkrvlgdsgeldilrqqmeerfvasnpskvsyepitttlrrkqeevsavvlqrayrghlarrgfickkttsnklenggthrekkestpstaslpsydsvtkpekekqqraeegrrerakrqkevreskc(配列番号26)
H.s.SCN9A
mamlpppgpqsfvhftkqslalieqriaerkskepkeekkdddeeapkpssdleagkqlpfiygdippgmvsepledldpyyadkktfivlnkgktifrfnatpalymlspfsplrrisikilvhslfsmlimctiltncifmtmnnppdwtknveytftgiytfeslvkilargfcvgeftflrdpwnwldfvvivfayltefvnlgnvsalrtfrvlralktisvipglktivgaliqsvkklsdvmiltvfclsvfaliglqlfmgnlkhkcfrnslennetlesimntleseedfrkyfyylegskdallcgfstdsgqcpegytcvkigrnpdygytsfdtfswaflalfrlmtqdywenlyqqtlraagktymiffvvviflgsfylinlilavvamayeeqnqanieeakqkelefqqmldrlkkeqeeaeaiaaaaaeytsirrsrimglsesssetsklssksakerrnrrkkknqkklssgeekgdaeklsksesedsirrksfhlgveghrrahekrlstpnqsplsirgslfsarrssrtslfsfkgrgrdigsetefaddehsifgdnesrrgslfvphrpqerrssnisqasrsppmlpvngkmhsavdcngvvslvdgrsalmlpngqllpegttnqihkkrrcssyllsedmlndpnlrqramsrasiltntveeleesrqkcppwwyrfahkfliwncspywikfkkciyfivmdpfvdlaiticivlntlfmamehhpmteefknvlaignlvftgifaaemvlkliamdpyeyfqvgwnifdslivtlslvelfladveglsvlrsfrllrvfklakswptlnmlikiignsvgalgnltlvlaiivfifavvgmqlfgksykecvckinddctlprwhmndffhsflivfrvlcgewietmwdcmevagqamclivymmvmvignlvvlnlflalllssfssdnltaieedpdannlqiavtrikkginyvkqtlrefilkafskkpkisreirqaedlntkkenyisnhtlaemskghnflkekdkisgfgssvdkhlmedsdgqsfihnpsltvtvpiapgesdlenmnaeelssdsdseyskvrlnrssssecstvdnplpgegeeaeaepmnsdepeacftdgcvrrfsccqvniesgkgkiwwnirktcykivehswfesfivlmillssgalafediyierkktikiileyadkiftyifilemllkwiaygyktyftnawcwldflivdvslvtlvantlgysdlgpikslrtlralrplralsrfegmrvvvnaligaipsimnvllvclifwlifsimgvnlfagkfyecinttdgsrfpasqvpnrsecfalmnvsqnvrwknlkvnfdnvglgylsllqvatfkgwtiimyaavdsvnvdkqpkyeyslymyiyfvvfiifgsfftlnlfigviidnfnqqkkklggqdifmteeqkkyynamkklgskkpqkpiprpgnkiqgcifdlvtnqafdisimvliclnmvtmmvekegqsqhmtevlywinvvfiilftgecvlklislrhyyftvgwnifdfvvviisivgmfladlietyfvsptlfrvirlarigrilrlvkgakgirtllfalmmslpalfniglllflvmfiyaifgmsnfayvkkedgindmfnfetfgnsmiclfqittsagwdgllapilnskppdcdpkkvhpgssvegdcgnpsvgifyfvsyiiisflvvvnmyiavilenfsvateesteplseddfemfyevwekfdpdatqfiefsklsdfaaaldpplliakpnkvqliamdlpmvsgdrihcldilfaftkrvlgesgemdslrsqmeerfmsanpskvsyepitttlkrkqedvsatviqrayrryrlrqnvknissiyikdgdrdddllnkkdmafdnvnensspektdatssttsppsydsvtkpdkekyeqdrtekedkgkdskeskk(配列番号27)
H.s.SCN10A
mefpigsletnnfrrftpeslveiekqiaakqgtkkarekhreqkdqeekprpqldlkacnqlpkfygelpaeligepledldpfysthrtfmvlnkgrtisrfsatralwlfspfnlirrtaikvsvhswfslfitvtilvncvcmtrtdlpekieyvftviytfealikilargfclneftylrdpwnwldfsvitlayvgtaidlrgisglrtfrvlralktvsvipglkvivgalihsvkkladvtiltifclsvfalvglqlfkgnlknkcvkndmavnettnysshrkpdiyinkrgtsdpllcgngsdsghcpdgyiclktsdnpdfnytsfdsfawaflslfrlmtqdswerlyqqtlrtsgkiymiffvlviflgsfylvnlilavvtmayeeqnqattdeieakekkfqealemlrkeqevlaalgidttslhshngspltsknaserrhrikprvsegstednksprsdpynqrrmsflglasgkrrashgsvfhfrspgrdislpegvtddgvfpgdheshrgslllgggagqqgplprsplpqpsnpdsrhgedehqppptselapgavdvsafdagqkktflsaeyldepfraqramsvvsiitsvleeleeseqkcppcltslsqkyliwdccpmwvklktilfglvtdpfaeltitlcivvntifmamehhgmsptfeamlqignivftifftaemvfkiiafdpyyyfqkkwnifdciivtvsllelgvakkgslsvlrsfrllrvfklakswptlntlikiignsvgalgnltiilaiivfvfalvgkqllgenyrnnrknisaphedwprwhmhdffhsflivfrilcgewienmwacmevgqksiclilfltvmvlgnlvvlnlfialllnsfsadnltapeddgevnnlqvalariqvfghrtkqalcsffsrscpfpqpkaepelvvklplssskaenhiaantargssgglqaprgprdehsdfianptvwvsvpiaegesdlddleddggedaqsfqqevipkgqqeqlqqvercgdhltprspgtgtssedlapslgetwkdesvpqvpaegvddtsssegstvdcldpeeilrkipeladdleepddcftegcirhcpcckldttkspwdvgwqvrktcyrivehswfesfiifmillssgslafedyyldqkptvkalleytdrvftfifvfemllkwvaygfkkyftnawcwldflivnislisltakileysevapikalrtlralrplralsrfegmrvvvdalvgaipsimnvllvclifwlifsimgvnlfagkfwrcinytdgefslvplsivnnksdckiqnstgsffwvnvkvnfdnvamgylallqvatfkgwmdimyaavdsrevnmqpkwednvymylyfvifiifggfftlnlfvgviidnfnqqkkklggqdifmteeqkkyynamkklgskkpqkpiprplnkfqgfvfdivtrqafditimvliclnmitmmvetddqseektkilgkinqffvavftgecvmkmfalrqyyftngwnvfdfivvvlsiaslifsailkslqsyfsptlfrvirlarigrilrliraakgirtllfalmmslpalfniglllflvmfiysifgmssfphvrweagiddmfnfqtfansmlclfqittsagwdgllspilntgppycdpnlpnsngtrgdcgspavgiiffttyiiisflimvnmyiavilenfnvateesteplseddfdmfyetwekfdpeatqfitfsalsdfadtlsgplripkpnrniliqmdlplvpgdkihcldilfaftknvlgesgeldslkanmeekfmatnlskssyepiattlrwkqedisatviqkayrsyvlhrsmalsntpcvpraeeeaaslpdegfvaftanencvlpdksetasatsfppsyesvtrglsdrvnmrtsssiqnedeatsmeliapgp(配列番号28)
H.s.SCN11A
mddrcypvifpdernfrpftsdslaaiekriaiqkekkkskdqtgevpqprpqldlkasrklpklygdipreligkpledldpfyrnhktfmvlnrkrtiyrfsakhalfifgpfnsirslairvsvhslfsmfiigtviincvfmatgpaknsnsnntdiaecvftgiyifealikilargfildefsflrdpwnwldsivigiaivsyipgitikllplrtfrvfralkaisvvsrlkvivgallrsvkklvnviiltffclsifalvgqqlfmgslnlkcisrdcknisnpeaydhcfekkenspefkmcgiwmgnsacsiqyeckhtkinpdynytnfdnfgwsflamfrlmtqdsweklyqqtlrttglysvfffivviflgsfylinltlavvtmayeeqnknvaaeieakekmfqeaqqllkeekealvamgidrssltsletsyftpkkrklfgnkkrksfflresgkdqppgsdsdedcqkkpqlleqtkrlsqnlsldhfdehgdplqrqralsavsiltitmkeqeksqepclpcgenlaskylvwnccpqwlcvkkvlrtvmtdpftelaiticiiintvflamehhkmeasfekmlnignlvftsifiaemclkiialdpyhyfrrgwnifdsivallsfadvmncvlqkrswpflrsfrvlrvfklakswptlntlikiignsvgalgsltvvlvivififsvvgmqlfgrsfnsqkspklcnptgptvsclrhwhmgdfwhsflvvfrilcgewienmwecmqeanassslcvivfilitvigklvvlnlfialllnsfsneerngnlegearktkvqlaldrfrrafcfvrhtlehfchkwcrkqnlpqqkevaggcaaqskdiiplvmemkrgsetqeelgiltsvpktlgvrhdwtwlaplaeeeddvefsgednaqritqpepeqqayelhqenkkptsqrvqsveidmfsedephltiqdprkksdvtsilsecstidlqdgfgwlpemvpkkqperclpkgfgccfpccsvdkrkppwviwwnlrktcyqivkhswfesfiifvillssgalifedvhlenqpkiqellnctdiifthifilemvlkwvafgfgkyftsawccldfiivivsvttlinlmelksfrtlralrplralsqfegmkvvvnaligaipailnvllvclifwlvfcilgvyffsgkfgkcingtdsvinytiitnksqcesgnfswinqkvnfdnvgnaylallqvatfkgwmdiiyaavdstekeqqpefesnslgyiyfvvfiifgsfftlnlfigviidnfnqqqkklggqdifmteeqkkyynamkklgskkpqkpiprplnkcqglvfdivtsqifdiiiisliilnmismmaesynqpkamksildhlnwvfvviftleclikifalrqyyftngwnlfdcvvvllsivstmistlenqehipfpptlfrivrlarigrilrlvraargirtllfalmmslpslfniglllflimfiyailgmnwfskvnpesgiddifnfktfassmlclfqistsagwdsllspmlrskescnsssenchlpgiatsyfvsyiiisflivvnmyiavilenfntateesedplgeddfdifyevwekfdpeatqfikysalsdfadalpeplrvakpnkyqflvmdlpmvsedrlhcmdilfaftarvlggsdgldsmkammeekfmeanplkklyepivtttkrkeeergaaiiqkafrkymmkvtkgdqgdqndlengphsplqtlcngdlssfgvakgkvhcd(配列番号29)
H.s.SCN1B
Mgrllalvvgaalvssacggcvevdseteavygmtfkilcisckrrsetnaetftewtfrqkgteefvkilryenevlqleederfegrvvwngsrgtkdlqdlsifitnvtynhsgdyechvyrllffenyehntsvvkkihievvdkanrdmasivseimmyvlivvltiwlvaemiycykkiaaatetaaqenaseylaitseskenctgvqvae(配列番号30)
H.s.SCN2B
Mhrdawlprpafsltglslffslvppgrsmevtvpatlnvlngsdarlpctfnscytvnhkqfslnwtyqecnncseemflqfrmkiinlklerfqdrvefsgnpskydvsvmlrnvqpedegiyncyimnppdrhrghgkihlqvlmeepperdstvavivgasvggflavvilvlmvvkcvrrkkeqklstddlkteeegktdgegnpddgak(配列番号31)
H.s.SCN3B
Mpafnrlfplaslvliywvsvcfpvcvevpseteavqgnpmklrciscmkreeveattvvewfyrpeggkdfliyeyrnghqevespfqgrlqwngskdlqdvsitvlnvtlndsglytcnvsrefefeahrpfvkttrliplrvteeagedftsvvseimmyillvfltlwlliemiycyrkvskaeeaaqenasdylaipsenkensavpvee(配列番号32)
H.s.SCN4B
Mpgagdggkaparwlgtgllglfllpvtlslevsvgkatdiyavngteillpctfsscfgfedlhfrwtynssdafkiliegtvkneksdpkvtlkdddritlvgstkekmnnisivlrdlefsdtgkytchvknpkennlqhhatiflqvvdrleevdntvtliilavvggvigllilillikkliifilkktrekkkeclvsssgndntenglpgskaeekppskv(配列番号33)
シグナル伝達プローブ3−(標的3に結合する)
5’−FamGCGAGAGCGACAAGCAGACCCTATAGAACCTCGCBHQ1クエンチ−3’(配列番号34)
Claims (34)
- アッセイにおいて少なくとも0.5のZ’ファクターを生じる、NaVを安定して発現するように改変された細胞又は細胞株。
- 前記Z’ファクターが少なくとも0.55、0.60、0.65、0.70、0.75、0.80、又は0.85である、請求項1に記載の細胞又は細胞株。
- 前記細胞又は細胞株が選択圧のない条件で生育する、請求項1に記載の細胞又は細胞株。
- 前記細胞又は細胞株が選択圧のない条件で少なくとも15日、30日、45日、60日、75日、100日、120日、又は150日の間NaVを発現する、請求項1に記載の細胞又は細胞株。
- 前記細胞又は細胞株が選択圧のない条件で一定のレベルのNaVを、少なくとも15日、30日、45日、60日、75日、100日、120日、又は150日の間発現する、請求項1に記載の細胞又は細胞株。
- 前記NaVがアルファサブユニット及びベータサブユニットを含む、請求項1に記載の細胞又は細胞株。
- 前記NaVが異なるベータサブユニットをさらに含む、請求項6に記載の細胞又は細胞株。
- 前記NaVが、サブユニットをコードする導入された核酸から発現するサブユニットを含む、請求項1に記載の細胞又は細胞株。
- 前記NaVが、遺伝子活性化によって活性化された内生の遺伝子から発現するNaVサブユニットを含む、請求項1に記載の細胞又は細胞株。
- 前記細胞又は細胞株が、改変に先立って内生NaVを発現しない、請求項1に記載の細胞又は細胞株。
- 前記NaVがいずれのポリペプチドタグをも含まない、請求項1に記載の細胞又は細胞株。
- 前記細胞又は細胞株が哺乳類である、請求項1に記載の細胞又は細胞株。
- 前記NaVが2つ、3つ、又は4つのサブユニットを含む、請求項1に記載の細胞又は細胞株。
- 前記NaVがヒトNaVである、請求項1に記載の細胞又は細胞株。
- 前記アルファサブユニットがアルファ1、アルファ2、アルファ3、アルファ4、アルファ5、アルファ7、アルファ8、アルファ9、アルファ10、又はアルファ11サブユニットである、請求項6に記載の細胞又は細胞株。
- 前記ベータサブユニットがベータ1、ベータ2、ベータ3、又はベータ4サブユニットである、請求項6に記載の細胞又は細胞株。
- 前記アルファサブユニットがヒトNaVアルファ9サブユニットである、請求項6に記載の細胞又は細胞株。
- 前記ベータサブユニットがヒトベータ1サブユニットである、請求項6又は16に記載の細胞又は細胞株。
- 前記NaVがヒトベータ2サブユニットをさらに含む、請求項18に記載の細胞又は細胞株。
- 前記細胞又は細胞株が異なる形状のNaVを発現するコレクション中に含まれる、請求項1に記載の細胞又は細胞株のコレクション。
- 前記細胞又は細胞株を、並行処理を可能にする同じ生理学的特性を有するように同調させた、請求項20に記載のコレクション。
- 前記細胞又は細胞株が全て同じ細胞型である、請求項20に記載のコレクション。
- 前記生理学的特性が成長速度である、請求項20に記載のコレクション。
- 前記生理学的特性が組織培養表面への付着である、請求項20に記載のコレクション。
- 前記生理学的特性がZ’ファクターである、請求項20に記載のコレクション。
- 前記生理学的特性がNaVの発現レベルである、請求項20に記載のコレクション。
- NaV調節因子を同定する方法であって、前記方法は、
請求項1〜19のいずれかに記載の細胞又は細胞株、又は請求項20〜26のいずれか1項に記載のコレクションに試験化合物を接触させる工程;及び
試験化合物に接触していない細胞と比較して、細胞におけるNaV機能の変化を検出する工程を含み、ここで前記機能の変化は該試験化合物がNaV調節因子であることを示す。 - 前記試験化合物が小分子、ポリペプチド、ペプチド、又は抗体若しくはその抗原結合部位である、請求項27に記載の方法。
- 前記試験化合物が化合物のライブラリーである、請求項27に記載の方法。
- 前記ライブラリーが小分子ライブラリー、組み合わせライブラリー、ペプチドライブラリー又は抗体ライブラリーである、請求項29に記載の方法。
- 前記検出工程が、膜電位アッセイ、電気生理学的アッセイ、及び結合アッセイから選択される、請求項27に記載の方法。
- 前記工程がハイスループット法で実施される、請求項27に記載の方法。
- 請求項27に記載の方法によって同定された調節因子。
- 長期にわたってNaVを一定のレベルで安定して発現するように改変された細胞であり、
a)NaVをコードするmRNAを発現する複数の細胞を準備する工程;
b)前記細胞を個別の培養ベッセル中に個々に分散させ、それにより複数の別個の細胞培養を準備する工程;
c)それぞれの別個の細胞培養における条件が実質的に同一であることを特徴とする自動化された細胞培養法を用いて、一連の所望の培養条件下で細胞を培養する工程(この培養期間においては別個の細胞培養当たりの細胞数は標準化され、及び別個の細胞培養は同じスケジュールで継代される);
d)NaVの発現を測定するために、別個の細胞培養を少なくとも2回アッセイする工程;及び
e)2回のアッセイの両方において一定のレベルでNaVを発現する別個の細胞培養を同定し、それにより前記細胞を得る工程、
を含む方法によって作出された細胞。
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ES2640817T3 (es) | 2017-11-06 |
MX2011008130A (es) | 2011-11-18 |
WO2010087864A1 (en) | 2010-08-05 |
JP5809065B2 (ja) | 2015-11-10 |
EP2391647B1 (en) | 2017-07-26 |
US20170219606A1 (en) | 2017-08-03 |
CA2751159A1 (en) | 2010-08-05 |
US20110312533A1 (en) | 2011-12-22 |
EP2391647A1 (en) | 2011-12-07 |
BRPI0924225A2 (pt) | 2016-10-11 |
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