JP2012515534A5 - - Google Patents
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- JP2012515534A5 JP2012515534A5 JP2011546450A JP2011546450A JP2012515534A5 JP 2012515534 A5 JP2012515534 A5 JP 2012515534A5 JP 2011546450 A JP2011546450 A JP 2011546450A JP 2011546450 A JP2011546450 A JP 2011546450A JP 2012515534 A5 JP2012515534 A5 JP 2012515534A5
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- Prior art keywords
- seq
- nucleotide sequence
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- nucleotide
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- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000002773 nucleotide Substances 0.000 claims description 562
- 125000003729 nucleotide group Chemical group 0.000 claims description 562
- 238000000034 method Methods 0.000 claims description 102
- 241000711549 Hepacivirus C Species 0.000 claims description 96
- 238000003752 polymerase chain reaction Methods 0.000 claims description 95
- 108020004707 nucleic acids Proteins 0.000 claims description 49
- 102000039446 nucleic acids Human genes 0.000 claims description 49
- 150000007523 nucleic acids Chemical class 0.000 claims description 49
- 238000010839 reverse transcription Methods 0.000 claims description 42
- 108020004414 DNA Proteins 0.000 claims description 20
- 239000011541 reaction mixture Substances 0.000 claims description 14
- 230000000295 complement effect Effects 0.000 claims description 12
- 108091093088 Amplicon Proteins 0.000 claims description 10
- 238000007862 touchdown PCR Methods 0.000 claims description 8
- 238000012163 sequencing technique Methods 0.000 claims description 7
- 101800001554 RNA-directed RNA polymerase Proteins 0.000 claims description 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical group C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 4
- 101710118188 DNA-binding protein HU-alpha Proteins 0.000 claims description 4
- 101710144128 Non-structural protein 2 Proteins 0.000 claims description 4
- 101710144111 Non-structural protein 3 Proteins 0.000 claims description 4
- 101800001020 Non-structural protein 4A Proteins 0.000 claims description 4
- 101800001019 Non-structural protein 4B Proteins 0.000 claims description 4
- 101800001014 Non-structural protein 5A Proteins 0.000 claims description 4
- 101710199667 Nuclear export protein Proteins 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 238000010367 cloning Methods 0.000 claims description 2
- 208000015181 infectious disease Diseases 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims 1
- 239000010703 silicon Substances 0.000 claims 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 14
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 8
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 8
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 8
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 2
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229960003237 betaine Drugs 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 108020005345 3' Untranslated Regions Proteins 0.000 description 1
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- 238000009007 Diagnostic Kit Methods 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- RFHAOTPXVQNOHP-UHFFFAOYSA-N fluconazole Chemical compound C1=NC=NN1CC(C=1C(=CC(F)=CC=1)F)(O)CN1C=NC=N1 RFHAOTPXVQNOHP-UHFFFAOYSA-N 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14608309P | 2009-01-21 | 2009-01-21 | |
| US61/146,083 | 2009-01-21 | ||
| PCT/US2010/021589 WO2010090857A2 (en) | 2009-01-21 | 2010-01-21 | Methods for amplifying hepatitis c virus nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012515534A JP2012515534A (ja) | 2012-07-12 |
| JP2012515534A5 true JP2012515534A5 (enExample) | 2013-03-07 |
Family
ID=42334055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011546450A Pending JP2012515534A (ja) | 2009-01-21 | 2010-01-21 | Ｃ型肝炎ウイルス核酸を増幅する方法 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US8679740B2 (enExample) |
| EP (1) | EP2389452A2 (enExample) |
| JP (1) | JP2012515534A (enExample) |
| KR (1) | KR20120101278A (enExample) |
| CN (1) | CN102341506A (enExample) |
| AU (1) | AU2010210892A1 (enExample) |
| CA (1) | CA2749969A1 (enExample) |
| IL (1) | IL214195A0 (enExample) |
| MX (1) | MX2011007693A (enExample) |
| NZ (1) | NZ594155A (enExample) |
| RU (1) | RU2011134857A (enExample) |
| WO (1) | WO2010090857A2 (enExample) |
| ZA (1) | ZA201105349B (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX352987B (es) | 2007-10-02 | 2017-12-15 | Theranos Ip Co Llc | Dispositivos modulares de punto de cuidado y usos de los mismos. |
| CA2825196C (en) | 2011-01-21 | 2021-01-05 | Theranos, Inc. | Systems and methods for sample use maximization |
| US9619627B2 (en) | 2011-09-25 | 2017-04-11 | Theranos, Inc. | Systems and methods for collecting and transmitting assay results |
| US9632102B2 (en) | 2011-09-25 | 2017-04-25 | Theranos, Inc. | Systems and methods for multi-purpose analysis |
| US8475739B2 (en) | 2011-09-25 | 2013-07-02 | Theranos, Inc. | Systems and methods for fluid handling |
| US20140170735A1 (en) | 2011-09-25 | 2014-06-19 | Elizabeth A. Holmes | Systems and methods for multi-analysis |
| US9268915B2 (en) | 2011-09-25 | 2016-02-23 | Theranos, Inc. | Systems and methods for diagnosis or treatment |
| US8840838B2 (en) | 2011-09-25 | 2014-09-23 | Theranos, Inc. | Centrifuge configurations |
| US9664702B2 (en) | 2011-09-25 | 2017-05-30 | Theranos, Inc. | Fluid handling apparatus and configurations |
| US9250229B2 (en) | 2011-09-25 | 2016-02-02 | Theranos, Inc. | Systems and methods for multi-analysis |
| US10012664B2 (en) | 2011-09-25 | 2018-07-03 | Theranos Ip Company, Llc | Systems and methods for fluid and component handling |
| US9810704B2 (en) | 2013-02-18 | 2017-11-07 | Theranos, Inc. | Systems and methods for multi-analysis |
| CN105683751B (zh) | 2013-09-06 | 2020-01-17 | 赛拉诺斯知识产权有限责任公司 | 用于接收拭子的装置、系统、方法和套件 |
| BR112016004994A2 (pt) | 2013-09-06 | 2021-05-11 | Theranos Ip Company, Llc | Sistemas e métodos para detectar doenças infecciosas |
| CN106222306A (zh) * | 2016-08-30 | 2016-12-14 | 合肥金域医学检验所有限公司 | 一种hcv病毒ns5a蛋白编码序列突变检测试剂盒 |
| GB201819726D0 (en) * | 2018-12-03 | 2019-01-16 | Diagnostics For The Real World Ltd | HCV detection |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
| US4973679A (en) | 1981-03-27 | 1990-11-27 | University Patents, Inc. | Process for oligonucleo tide synthesis using phosphormidite intermediates |
| DE3329892A1 (de) | 1983-08-18 | 1985-03-07 | Köster, Hubert, Prof. Dr., 2000 Hamburg | Verfahren zur herstellung von oligonucleotiden |
| FR2596761B1 (fr) | 1986-04-08 | 1988-05-20 | Commissariat Energie Atomique | Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides |
| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
| EP0787807B1 (en) | 1991-08-27 | 2003-05-28 | F. Hoffmann-La Roche Ag | Primers and probes for hepatitis C detection |
| EP0532258A2 (en) * | 1991-09-09 | 1993-03-17 | Immuno Japan Inc. | Oligonucleotides and determination system of HCV genotypes |
| JPH0775585A (ja) * | 1993-06-14 | 1995-03-20 | Immuno Japan:Kk | Ｃ型肝炎ウイルス関連オリゴヌクレオチドならびにｃ型肝炎 ウイルス遺伝子型判定方法 |
| ZA966663B (en) | 1995-08-17 | 1998-02-06 | Genentech Inc | Traf Inhibitors. |
| US6153421A (en) * | 1997-07-18 | 2000-11-28 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Cloned genomes of infectious hepatitis C viruses and uses thereof |
| WO2002064842A2 (en) * | 2001-02-13 | 2002-08-22 | Children`S Hospital Research Foundation | Quantitative epstein barr virus pcr rapid assay |
| JP3751867B2 (ja) * | 2001-09-18 | 2006-03-01 | 株式会社東芝 | インターフェロンを投与されるべき個体においてインターフェロン療法の有効性を予測する方法、その方法をコンピュータにより実行させるためのプログラム、インターフェロン感受性に関連する多型部位の遺伝子型を検出するための核酸プローブ、およびその核酸プローブを具備する塩基配列検出用チップ |
| AU2003230790A1 (en) * | 2002-04-03 | 2003-10-20 | Smithkline Beecham Corporation | Hepatitis c virus sub-genomic replicons |
| US20040137539A1 (en) * | 2003-01-10 | 2004-07-15 | Bradford Sherry A. | Cancer comprehensive method for identifying cancer protein patterns and determination of cancer treatment strategies |
| WO2004073599A2 (en) | 2003-02-18 | 2004-09-02 | Pfizer Inc. | Inhibitors of hepatitis c virus, compositions and treatments using the same |
| US20050282179A1 (en) | 2003-12-05 | 2005-12-22 | Applera Corporation | Biomarkers for interferon-alpha response in hepatitis C virus infected patients |
| JPWO2006085407A1 (ja) * | 2005-02-09 | 2008-12-18 | 学校法人日本大学 | Ｈｃｖ量に関連する遺伝子のスクリーニング方法 |
| EP2711427A1 (en) * | 2007-04-27 | 2014-03-26 | Advanced Life Science Institute, Inc. | HCV gene |
| JP4213759B2 (ja) * | 2007-08-10 | 2009-01-21 | 株式会社東芝 | 標的核酸の遺伝子型、および変異の判定方法、不溶性支持体に核酸断片を固定化する方法、所望の核酸を精製し、かつ増幅するための方法、並びに遺伝子型アッセイキット |
-
2010
- 2010-01-21 AU AU2010210892A patent/AU2010210892A1/en not_active Abandoned
- 2010-01-21 CN CN2010800098527A patent/CN102341506A/zh active Pending
- 2010-01-21 NZ NZ594155A patent/NZ594155A/xx not_active IP Right Cessation
- 2010-01-21 CA CA2749969A patent/CA2749969A1/en not_active Abandoned
- 2010-01-21 JP JP2011546450A patent/JP2012515534A/ja active Pending
- 2010-01-21 WO PCT/US2010/021589 patent/WO2010090857A2/en not_active Ceased
- 2010-01-21 KR KR1020117019326A patent/KR20120101278A/ko not_active Withdrawn
- 2010-01-21 MX MX2011007693A patent/MX2011007693A/es not_active Application Discontinuation
- 2010-01-21 EP EP10703544A patent/EP2389452A2/en not_active Ceased
- 2010-01-21 RU RU2011134857/10A patent/RU2011134857A/ru not_active Application Discontinuation
-
2011
- 2011-07-19 IL IL214195A patent/IL214195A0/en unknown
- 2011-07-20 ZA ZA2011/05349A patent/ZA201105349B/en unknown
- 2011-07-20 US US13/186,985 patent/US8679740B2/en not_active Expired - Fee Related
-
2014
- 2014-01-28 US US14/165,757 patent/US20140199684A1/en not_active Abandoned
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