JP2012509084A - ポリヌクレオチドアセンブリに関する組成物及び方法 - Google Patents

ポリヌクレオチドアセンブリに関する組成物及び方法 Download PDF

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JP2012509084A
JP2012509084A JP2011537596A JP2011537596A JP2012509084A JP 2012509084 A JP2012509084 A JP 2012509084A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2011537596 A JP2011537596 A JP 2011537596A JP 2012509084 A JP2012509084 A JP 2012509084A
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nucleic acid
annealable linker
restriction site
restriction
linker sequence
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JP2012509084A5 (cg-RX-API-DMAC7.html
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セルベル ザクフ
ロウエ ライモンド
エー． ウベルサク ジェエフフレイ
エス． チャンドラン スニル
ジェエデドイアフ デアン エリク
エム． プルアトト ダルレン
トシキ タケオカ ケンネトフ
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アムイリス， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2011537596A 2008-11-19 2009-11-19 ポリヌクレオチドアセンブリに関する組成物及び方法 Pending JP2012509084A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11610908P 2008-11-19 2008-11-19
US61/116,109 2008-11-19
US16223009P 2009-03-20 2009-03-20
US61/162,230 2009-03-20
PCT/US2009/065048 WO2010059763A1 (en) 2008-11-19 2009-11-19 Compositions and methods for the assembly of polynucleotides

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JP2012509084A true JP2012509084A (ja) 2012-04-19
JP2012509084A5 JP2012509084A5 (cg-RX-API-DMAC7.html) 2012-10-11

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EP (1) EP2358875B1 (cg-RX-API-DMAC7.html)
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KR (1) KR101794298B1 (cg-RX-API-DMAC7.html)
CN (1) CN102282255B (cg-RX-API-DMAC7.html)
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BR (1) BRPI0922187B1 (cg-RX-API-DMAC7.html)
CA (1) CA2744153C (cg-RX-API-DMAC7.html)
MX (1) MX2011005195A (cg-RX-API-DMAC7.html)
SG (1) SG171760A1 (cg-RX-API-DMAC7.html)
WO (1) WO2010059763A1 (cg-RX-API-DMAC7.html)
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015521850A (ja) * 2012-06-29 2015-08-03 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
JP2021151200A (ja) * 2020-03-24 2021-09-30 国立大学法人 東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ

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US7919605B1 (en) 2010-08-30 2011-04-05 Amyris, Inc. Nucleic acids, compositions and methods for the excision of target nucleic acids
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WO2013144257A1 (en) * 2012-03-27 2013-10-03 Dsm Ip Assets B.V. Cloning method
US9976162B2 (en) 2012-12-10 2018-05-22 Agilent Technologies, Inc. Pairing code directed assembly
CN105189772B (zh) 2013-03-15 2019-09-13 阿迈瑞斯公司 磷酸转酮酶和磷酸转乙酰酶生产乙酰-辅酶a衍生化合物的用途
CN105408352B (zh) 2013-04-18 2020-01-14 泰莱托恩基金会 通过双重aav载体有效递送大基因
MX2016007797A (es) 2013-12-19 2016-09-07 Amyris Inc Metodos para integracion genomica.
US10287627B2 (en) 2014-02-08 2019-05-14 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking PCR
WO2015184016A2 (en) * 2014-05-27 2015-12-03 The Broad Institute, Inc. High-thoughput assembly of genetic elements
US11518797B2 (en) 2014-11-11 2022-12-06 Clara Foods Co. Methods and compositions for egg white protein production
US20180127804A1 (en) 2014-12-05 2018-05-10 Amyris, Inc. High-throughput sequencing of polynucleotides
SI3265571T1 (sl) * 2015-03-03 2022-10-28 Fondazione Telethon Več vektorski sistem in njegove uporabe
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
WO2017024173A1 (en) 2015-08-04 2017-02-09 The Regents Of The University Of Colorado Multiplexed binary assembly and quantitative tracking of bacterial populations
EP3368673B1 (en) 2015-10-29 2020-07-29 Amyris, Inc. Compositions and methods for production of myrcene
CN106811459B (zh) * 2015-11-27 2019-09-27 清华大学 一种利用短链引物阻遏提高dna克隆及组装效率的方法
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
JP6821598B2 (ja) 2015-12-07 2021-01-27 ザイマージェン インコーポレイテッド Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーター
US11293033B2 (en) 2016-05-18 2022-04-05 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
US10544411B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a glucose permease library and uses thereof
EP3478833A4 (en) 2016-06-30 2019-10-02 Zymergen, Inc. METHOD FOR PRODUCING A BACTERIAL HEMOGLOBIN LIBRARY AND ITS USE
GB201621589D0 (en) * 2016-12-19 2017-02-01 Univ Gent And Katholieke Univ Leuven K U Leuven R&D Polynucleotide shuffling method
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AU2018291041B2 (en) 2017-06-30 2021-08-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019055535A1 (en) 2017-09-18 2019-03-21 Amyris, Inc. METHODS OF GENOMIC INTEGRATION FOR HOST CELLS COMPRISING HANSENULA AND ARXULA
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US10376889B1 (en) 2018-04-13 2019-08-13 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
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US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
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WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
AU2019292919A1 (en) 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
CA3108892A1 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
US11066663B2 (en) * 2018-10-31 2021-07-20 Zymergen Inc. Multiplexed deterministic assembly of DNA libraries
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US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
AU2020309602B2 (en) 2019-07-11 2025-05-15 Clara Foods Co. Protein compositions and consumable products thereof
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
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US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
WO2021211972A1 (en) * 2020-04-16 2021-10-21 Zymergen Inc. Circular-permuted nucleic acids for homology-directed editing
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
JP2003533979A (ja) * 2000-03-07 2003-11-18 ユニバーシティー オブ ゲルフ Ｄｎａの連結方法
WO2007021944A2 (en) * 2005-08-11 2007-02-22 The J. Craig Venter Institute In vitro recombination method
WO2007032837A2 (en) * 2005-08-11 2007-03-22 The J. Craig Venter Institute Method for in vitro recombination
WO2008045380A2 (en) * 2006-10-04 2008-04-17 Codon Devices, Inc. Nucleic acid libraries and their design and assembly
WO2009048885A2 (en) * 2007-10-08 2009-04-16 Synthetic Genomics, Inc. Assembly of large nucleic acids

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5057419A (en) * 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
AU9577998A (en) 1997-09-29 1999-04-23 City Of Hope Efficient linking of nucleic acid segments
ES2324513T3 (es) * 1999-03-18 2009-08-10 Complete Genomics As Procedimientos de clonacion y produccion de cadenas de fragmentos con contenido de informacion legible.
WO2002016594A2 (en) * 2000-08-21 2002-02-28 Invitrogen Corporation Methods and reagents for molecular cloning
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics
US20020165175A1 (en) 2001-04-17 2002-11-07 Xiaowu Liang Fast and enzymeless cloning of nucleic acid fragments
CN1263860C (zh) * 2002-09-30 2006-07-12 华南农业大学 多基因载体的构建方法与应用
US20080050808A1 (en) * 2002-10-09 2008-02-28 Reed Thomas D DNA modular cloning vector plasmids and methods for their use
AU2008212907A1 (en) * 2007-02-05 2008-08-14 Philipps-Universitat Marburg Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases
JP2012509084A (ja) * 2008-11-19 2012-04-19 アムイリス， インコーポレイテッド ポリヌクレオチドアセンブリに関する組成物及び方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
JP2003533979A (ja) * 2000-03-07 2003-11-18 ユニバーシティー オブ ゲルフ Ｄｎａの連結方法
WO2007021944A2 (en) * 2005-08-11 2007-02-22 The J. Craig Venter Institute In vitro recombination method
WO2007032837A2 (en) * 2005-08-11 2007-03-22 The J. Craig Venter Institute Method for in vitro recombination
WO2008045380A2 (en) * 2006-10-04 2008-04-17 Codon Devices, Inc. Nucleic acid libraries and their design and assembly
WO2009048885A2 (en) * 2007-10-08 2009-04-16 Synthetic Genomics, Inc. Assembly of large nucleic acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6014010159; Ｓｃｉｅｎｃｅ ｖｏｌ．３１９，　ｎｏ．５８６７, 20080124, ｐｐ．１２１５-１２２０ *
JPN6014051321; Ｔｒｅｎｄｓ　Ｂｉｏｔｅｃｈｎｏｌ． ｖｏｌ．２３，　ｎｏ．４, 2005, ｐｐ．１９９-２０７ *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015521850A (ja) * 2012-06-29 2015-08-03 マサチューセッツ インスティテュート オブ テクノロジー 超並列コンビナトリアル遺伝学
JP2021151200A (ja) * 2020-03-24 2021-09-30 国立大学法人 東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ
WO2021192596A1 (ja) * 2020-03-24 2021-09-30 国立大学法人東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ
JP7473953B2 (ja) 2020-03-24 2024-04-24 国立大学法人 東京大学 連結ｄｎａの製造方法及びそれに用いるためのベクターの組み合わせ

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KR20110102351A (ko) 2011-09-16
WO2010059763A1 (en) 2010-05-27
CA2744153C (en) 2020-07-07
EP2358875A1 (en) 2011-08-24
US20100124768A1 (en) 2010-05-20
AU2009316660A1 (en) 2011-07-07
KR101794298B1 (ko) 2017-11-20
ZA201103637B (en) 2012-08-29
US8546136B2 (en) 2013-10-01
US8110360B2 (en) 2012-02-07
BRPI0922187B1 (pt) 2021-05-04
EP2358875B1 (en) 2015-08-12
BRPI0922187A2 (pt) 2018-10-23
AU2009316660B2 (en) 2015-01-29
CN102282255B (zh) 2014-05-07
SG171760A1 (en) 2011-07-28
US20100136633A1 (en) 2010-06-03
CA2744153A1 (en) 2010-05-27
MX2011005195A (es) 2011-09-01
US8221982B2 (en) 2012-07-17
CN102282255A (zh) 2011-12-14
US20120245056A1 (en) 2012-09-27

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