JP2012140487A - Enzyme-containing cleanser composition for endoscope-cleansing machine - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an enzyme-containing cleanser composition for endoscope-cleansing machines, little in foaming even at low temperature and excellent in cleansing force, and to provide a method for cleansing an endoscope.SOLUTION: The enzyme-containing cleanser composition for the endoscope-cleansing machines contains (A) 1-50 mass% of a surfactant and (B) a protease (hereinafter referred to as a component (B)), wherein, the rate of a non-ionic surfactant represented by general formula (I) below in the surfactant is ≥90 mass%: R-O-[(EO)/(PO)]-H (I), wherein R is 7-9C branched alkyl; EO is ethanediyloxy; PO is propanediyloxy; m and n are average addition molar numbers; m is a number of 1-30; n is a number of 2-50; "/" is a symbol showing that EO and PO may be random or block; the addition order of EO and PO is not asked.

Description

  The present invention relates to an enzyme-containing cleaning composition for an endoscope cleaner and a method for cleaning an endoscope.

  Conventionally, as a reprocessing method after use, an endoscope has been used for the next treatment after being thoroughly cleaned and disinfected. Although there is an automatic endoscope cleaning machine for cleaning endoscopes, it is necessary to wash by hand before using the endoscope cleaning machine due to insufficient cleaning power. Met. One reason for this is thought to be that foaming occurs during cleaning, and physical force due to ultrasonic waves or water flow cannot be applied to the endoscope. In addition, dirt that adheres to the surface, such as coagulated blood, can be washed to some extent with visible dirt on the surface, but residual proteins such as fibrin cannot be removed with ordinary detergents. Another cause is that this residual protein may cause disinfection failure in the disinfection process after cleaning.

  Patent Document 1 discloses a specific endoscope cleaning method using a cleaning liquid containing a polyoxyethylene-polyoxypropylene nonionic surfactant. Patent Document 1 describes that a cleaning liquid in which an endoscope is immersed is circulated by a circulating liquid discharging means existing below the surface of the cleaning liquid. Patent Document 2 discloses a method of cleaning an endoscope tube by a gas-liquid two-phase flow. Patent Document 3 discloses a cleaning composition for medical devices containing a specific polyoxyethylene-polyoxypropylene block polymer, a specific polyoxyalkylene alkyl ether, an alkanolamine, and a specific inorganic alkaline agent under specific conditions. Is disclosed. Patent Document 4 includes a liquid medium, an enzyme composition, a specific dispersible stabilizing component, and a specific surfactant, and is substantially free of alkali metal hydroxide or an active chlorine source. A chemical enzyme-containing detergent composition is disclosed.

JP 2010-57846 A International Publication No. 2010/39736 Pamphlet JP 2009-144070 A Japanese National Patent Publication No. 10-505374

  In washing with an endoscope automatic washer, it is common to use tap water at the same temperature without heating, but the surfactant of general formula (I) in Patent Document 1 has a low water temperature. In addition, significant foaming occurs and the cleaning properties are reduced. Also, in the cited document 1, it is described that the discharge nozzle for circulation is provided in water, that is, below the surface of the cleaning liquid. However, when the nozzle is installed below the liquid surface, the state of the water flow should be confirmed visually. Therefore, it is difficult to notice even if the cleaning performance deteriorates due to clogging of the nozzles, which causes poor cleaning, and the water flow does not reach the endoscope cover, etc., and dirt easily accumulates in the cleaning machine. . Moreover, the method of patent document 2 wash | cleans the inside of the pipe line of an endoscope, and cannot be applied to an outer surface. Moreover, the detergent composition of Patent Document 3 cannot wash the coagulated blood adhering to the endoscope detergent to the invisible residual protein portion. Moreover, in the washing | cleaning in the tableware processing field like patent document 4, although warm water is generally used, in the washing | cleaning by an endoscope washing machine, it is often used without heating tap water etc., and low temperature This causes foaming problems. As described above, a cleaning agent for an automatic endoscope cleaning machine that has low foaming even at low temperatures and has excellent cleaning power has not been found.

  An object of the present invention is to provide an enzyme-containing cleaning composition for an endoscope cleaning machine and a method for cleaning an endoscope that have less foaming even at a low temperature and have excellent cleaning power.

The present invention is an enzyme-containing cleaning composition for an endoscope cleaning machine, which comprises (A) a surfactant (hereinafter referred to as (A) component) in an amount of 1 to 50% by mass and (B) a protease (hereinafter referred to as ( B) component] and the proportion of the nonionic surfactant represented by the following general formula (I) [hereinafter referred to as component (A1)] is 90% by mass or more. The present invention relates to an enzyme-containing cleaning composition for an endoscope cleaning machine.
R—O — [(EO) _m / (PO) _n ] —H (I)
[Wherein, R is a branched alkyl group having 7 to 9 carbon atoms, EO is an ethanediyloxy group, PO is a propanediyloxy group, m and n are average added mole numbers, m is a number of 1 to 30, n is a number from 2 to 50. “/” Is a symbol indicating that EO and PO may be random or block. The order of adding EO and PO is not limited. ]

  In the present invention, the enzyme-containing cleaning composition for an endoscope cleaning machine means a cleaning composition that is used in an endoscope cleaning machine and contains an enzyme. An enzyme-containing cleaning composition for cleaning an endoscope.

  The present invention also relates to a method for cleaning an endoscope with an endoscope cleaning machine using a diluted cleaning solution obtained by diluting the enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention with water 50 to 1000 times. Further, the present invention relates to a cleaning method for cleaning an endoscope with a water flow of the diluted cleaning liquid.

  According to the present invention, there are provided an enzyme-containing cleaning composition for an endoscope cleaner and a method for cleaning an endoscope, which are less foamed even at a low temperature and have excellent cleaning power.

<(A) component>
The component (A) is a surfactant. As the component (A), any of a nonionic surfactant, an anionic surfactant, a cationic surfactant, and a zwitterionic surfactant can be used. In many cases, the temperature of the water is not controlled, and when it is washed with room temperature or warm water, the bubbles are difficult to disappear when the water temperature is low, even if the bubbles are not a problem.

  On the other hand, in order to increase the cleaning power, the water jetted at a high pressure is constantly circulating in the endoscope cleaning machine, which makes it very easy to foam. When the bubbles build up, the physical force of the ultrasonic waves and water flow is relaxed by the bubbles, and it becomes difficult to be transmitted to the endoscope surface and the cleaning power is reduced. Not only that, the water level sensor for detecting the supply and discharge of the cleaning water provided in the endoscope cleaning machine causes a false detection and the cleaning stops. The same problem is also observed when extremely low hardness water such as RO water or ion exchange water is used. Therefore, it is necessary that foaming is suppressed even when water having a low hardness of 5 ° C. is used. Under such conditions, almost all surfactants have high foaming properties and are not suitable for cleaning. On the other hand, if a surfactant having a very low foaming property is used, the cleaning power is too weak to be suitable for use.

Only when a certain amount or more of the component (A1) is used as the component (A) as in the present invention, it is possible to reduce both foaming and cleaning power at a low temperature. Moreover, when other types of surfactants are mixed even in a small amount, foaming increases or the cleaning power decreases, so that most of the surfactants in the cleaning composition are composed of the component (A1). It must consist only of specific surfactants. Specifically, the proportion of the nonionic surfactant represented by the general formula (I) must be 90% by mass or more in the component (A). Moreover, it is preferable that it is 95 mass% or more, and it is more preferable that it is 99 mass% or more.
R—O — [(EO) _m / (PO) _n ] —H (I)
[Wherein, R is a branched alkyl group having 7 to 9 carbon atoms, EO is an ethanediyloxy group, PO is a propanediyloxy group, m and n are average added mole numbers, m is a number of 1 to 30, n is a number from 2 to 50. “/” Is a symbol indicating that EO and PO may be random or block. The order of adding EO and PO is not limited. ]

  From the viewpoint of detergency, the carbon number of R is preferably 8-9. Moreover, in the point which suppresses foaming at low temperature, m is preferably 1-20, more preferably 2-15, and particularly preferably 3-10. N is preferably from 3 to 20, particularly preferably from 4 to 10. EO and PO are preferably random adducts.

  Since the surfactant of the general formula (I) has a low cloud point, it can be easily separated at a high temperature. Therefore, other types of surfactants can be used in combination, but other types can be used to suppress foaming. The surfactant should be 10% by mass or less based on the total surfactant.

<(B) component>
Component (B) is a protease. The protease may be any enzyme as long as it has an optimum pH from neutral to alkaline, and a plurality of proteases satisfying this condition can be used in combination. The component (D) of the present invention is preferably a subtilisin protease derived from Bacillus SP, and among them, a subtilisin protease derived from Bacillus Halodurans or Bacillus clausii is preferred. Examples of commercially available alkaline proteases include Alcalase, Sabinase, Evalase, Esperase, Cannase, Ovozyme, and Perfect, Properase available from Genencor International, available from Novozymes Japan. In addition, proteases described in JP-A-2007-61101 can also be suitably used.

  In addition, in the diluted cleaning solution obtained by diluting the enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention 50 to 1000 times with water, the content (proteolytic activity) of the component (B) is the fixed protein removal effect and From a viewpoint of cost, 0.01-200 PU is preferable per 1 g of cleaning agents, 0.05-100 PU is more preferable, 0.1-50 PU is further more preferable, 0.5-20 PU is especially preferable. The content of the component (B) in the enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention can be appropriately determined in consideration of the content in such a diluted cleaning solution.

The proteolytic activity (PU / g) is measured by the following method.
1 mL of 50 mmol / L borate buffer solution (pH 10.5) containing casein (Hammerstein: Merck) at a concentration of 1 w / v% was kept at 30 ° C. for 5 minutes, and then mixed with 0.1 g of enzyme solution. The reaction is performed at 30 ° C. for 15 minutes. Add 2 mL of the reaction stop solution (0.11 mol / L trichloroacetic acid-0.22 mol / L sodium acetate-0.33 mol / L acetic acid) and let stand at room temperature for 10 minutes. Next, the acid-denatured protein was filtered (No. 2 filter paper; manufactured by Whatman), and an alkaline copper solution [1 w / v% potassium tartrate / sodium tartrate aqueous solution: 1 w / v% copper sulfate aqueous solution: sodium carbonate was added to 0.5 mL of the filtrate. 0.1 mol / L aqueous sodium hydroxide solution (sodium carbonate concentration 2 w / v%) = 1: 1: 100 (V / V)] 2.5 mL is added, and the mixture is kept at 30 ° C. for 10 minutes. Further, 0.25 mL of a diluted phenol reagent [phenol reagent (manufactured by Kanto Chemical Co., Ltd.) diluted twice with ion-exchanged water] is added, and the mixture is incubated at 30 ° C. for 30 minutes, and then the absorbance at 660 nm of this sample is measured. Moreover, after mixing a reaction stop liquid with said enzyme reaction system, what added an enzyme solution is measured similarly as a blank. Next, the amount of acid-soluble proteolysate that has been liberated (the amount converted to tyrosine) is obtained from the difference in absorbance between the sample and the blank, and this is the reaction time (in this case: 15 minutes) and the amount of enzyme solution (this In the case of conditions: Dividing by 0.1 g), the proteolytic activity value can be determined. Note that 1 PU is the amount of enzyme that liberates an acid-soluble proteolysate corresponding to 1 mmol of tyrosine per minute under the above reaction conditions.

<(C) component>
The enzyme-containing cleaning composition for an endoscope cleaner according to the present invention preferably contains a polyhydric alcohol [component (C)].

  The component (A1) is a surfactant that has a low foaming property but has a low cloud point and is easily separated at a high temperature. When other surfactants are added to solubilize the component (A1) at a high temperature, foaming problems at low temperatures occur, and when a general hydrotrope such as sodium paratoluenesulfonate is added, Stability is reduced. However, blending polyhydric alcohol can improve blending stability at high temperatures without affecting foaming or enzyme stability.

  The polyhydric alcohol of component (C) is a compound that has two or more hydroxy groups in the molecule and does not contain a nitrogen atom. Specific examples include those having a basic skeleton of a straight chain, branched chain or cyclic hydrocarbon having 2 to 10 carbon atoms, or those having a sugar skeleton as a basic skeleton, and at least two hydrogen atoms or more. Is substituted with a hydroxyl group, or 1 to 4 molecules thereof are condensed by an ether bond. The polyhydric alcohol of the present invention can have other functional groups such as a ketone group and an aldehyde group, but preferably has no other functional group other than a hydroxyl group and an ether group. As a polyhydric alcohol, what has a C3-C6 linear hydrocarbon as a basic skeleton is preferable. Specific polyhydric alcohols include ethylene glycol, propylene glycol, dipropylene glycol, 1,3-butanediol, 1,2-butanediol, dibutylene glycol, 2,4-pentanediol, and 1.2-pentanediol. 1,5-pentanediol, 3-methyl 2,4-pentanediol, 1,6-hexanediol, 1,2-hexanediol, glycerol monoalkyl ether, glycerol, 1,2,3-hexanetriol, sorbitol, Examples include xylitol, glucose, erythritol, pentaerythritol, trehalose, maltitol, sucralose, inositol, diglycerin, triglycerin, tetraglycerin, and cyclohexanetetraol. More preferably, it has 3 to 6 carbon atoms and two hydroxyl groups. Particularly preferred are propylene glycol, 1,3 butanediol, and dipropylene glycol. The blending amount of the polyhydric alcohol is preferably 5 to 80% by mass, and preferably 10 to 80% by mass in the enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention in terms of solubilizing the component (A1). Is more preferable, 20-70 mass% is still more preferable, and 30-60 mass% is still more preferable. The mass ratio of component (A) / component (C), preferably the mass ratio of component (A1) / component (C) is preferably 1/1 or more, more preferably 1/1 to 1/20, still more preferably. Is 1/2 to 1/20, more preferably 1/3 to 1/10.

<(D) component>
The enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention preferably contains an alkaline agent (component (D)).

Detergency can be further improved by adding an alkaline agent to the cleaning composition of the present invention. As an alkaline agent, it is possible to mix | blend 1 or more types chosen from organic alkali compounds, such as an alkanolamine, an alkylamine, and quaternary ammonium, an alkali metal hydroxide, carbonate, phosphate, and silicate. Alkali metal hydroxides, carbonates, phosphates and silicates include potassium hydroxide, sodium hydroxide, potassium carbonate, sodium carbonate, potassium phosphate, sodium phosphate, No. 1 potassium silicate, No. 1 sodium silicate Examples include No. 2 potassium silicate, No. 2 sodium silicate, ortho orthosilicate, and ortho orthosilicate, and alkanolamine is preferable. Examples of the alkanolamine include those represented by the general formula N (R ¹ ) (R ² ) (R ³ ). R ¹ is a hydrocarbon group having 1 to 8 carbon atoms containing 1 to 3 OH groups, and R ² and R ³ are each independently a hydrogen atom, an alkyl group having 1 to 4 carbon atoms or 1 to 1 carbon atoms. 4 alkanol groups. R ¹ is preferably a C 2-4 alkanol group, and R ² and R ³ are preferably hydrogen atoms. Examples of the alkanolamine of the general formula include monoethanolamine, monopropanolamine, monoisopropanolamine, diethanolamine, triethanolamine, N-methylpropanolamine, N-dimethylethanolamine, 2-amino-2-methyl-1-propanol, Among these, monoethanolamine, monopropanolamine, monoisopropanolamine, and trishydroxyaminomethane are preferable from the viewpoint of detergency, and monoethanolamine is most preferable. As a compounding quantity of an alkaline agent, 1-30 mass% is preferable in the enzyme-containing cleaning composition for endoscope washing machines of this invention, 2-20 mass% is more preferable, 5-15 mass% is especially preferable. .

<Other ingredients>
The enzyme-containing cleaning composition for endoscope cleaning machine (high concentration product) of the present invention is a metal sequestering agent, solvent, hydrotrope agent, dispersant, pH adjuster, as long as the object of the present invention is not impaired. Thickeners, viscosity modifiers, fragrances, colorants, antioxidants, preservatives, foam inhibitors, bleaches, bleach activators, and the like can be blended. You may mix | blend these components with the washing | cleaning liquid prepared by diluting this cleaning composition.

  The enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention can contain a metal sequestering agent. By containing a metal sequestering agent, protein soil bound and fixed by alkaline earth metal ions or alkaline earth metal salts can be more efficiently washed.

  As the metal sequestering agent, any of aminocarboxylic acid type, organic acid type, phosphonic acid type, phosphoric acid type and polycarboxylic acid type can be used. For example, aminopolyacetic acid such as nitrilotriacetic acid, iminodiacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, glycol etherdiaminetetraacetic acid, hydroxyethyliminodiacetic acid, triethylenetetraaminehexaacetic acid, diencoric acid, etc. Glycolic acid, oxydisuccinic acid, carboxymethyloxysuccinic acid, citric acid, lactic acid, tartaric acid, oxalic acid, malic acid, gluconic acid, carboxymethyl succinic acid, carboxymethyl tartaric acid, glutamic acid diacetic acid, etc., or salts thereof, Phosphonic acids or salts thereof such as aminotri (methylenephosphonic acid), 1-hydroxyethylidene-1,1-diphosphonic acid, ethylenediaminetetra (methylenephosphonic acid), diethylenetriaminepenta (methylenephosphonic acid), Phosphoric acid or a salt thereof, such as phosphoric acid, polyacrylic acid, polyacrylic acid, polycarboxylic acid or a salt thereof and the like.

  The counter ions of these salts include alkali metals, quaternary amines, alkanolamines, etc., but alkanolamine salts are preferred from the viewpoint of anticorrosive properties for medical devices. Furthermore, monoethanolamine salts are preferred. When an alkanolamine salt type compound is used as the metal sequestering agent, the amount of alkanolamine alone is handled as part of the component (D).

  The metal sequestering agent is preferably 0.002 to 0.5 mass in the cleaning liquid prepared from the enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention of the present invention from the viewpoint of the effect of removing protein stains and cost. %, More preferably 0.005 to 0.3% by mass, still more preferably 0.01 to 0.2% by mass, and still more preferably 0.02 to 0.1% by mass. preferable.

  Moreover, the enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention contains a water-soluble calcium salt, boric acid or a salt thereof, a boron compound such as borax, formic acid or a salt thereof as an enzyme stabilizer. Can do.

  Solvents include monohydric alcohols such as ethanol and propanol, glycol ethers such as ethylene glycol ethyl ether, propylene glycol ethyl ether, ethylene glycol butyl ether, and diethylene glycol butyl ether. Hydrotropes include para-toluenesulfonic acid, benzoic acid, Xylenesulfonic acid or a salt thereof, urea, etc., as a dispersant, polyvinylpyrrolidone, as an antioxidant, butylhydroxytoluene, sodium sulfite, sodium hydrogen sulfite, as a foam inhibitor, polypropylene glycol having an average molecular weight of 500 to 10,000, Examples thereof include polypropylene glycol alkyl ethers having 8 to 18 carbon atoms and an average number of moles of added polypropylene glycol of 1 to 10, silicone, silica and the like. Examples of the pH adjuster include gluconic acid, malic acid, succinic acid, acetic acid and the like. In addition, although the compound applicable to (C) component can also be used as a solvent, the amount of the said compound is counted in the quantity of (C) component in that case.

  In the enzyme-containing cleaning composition for an endoscope cleaning machine of the present invention, the pH at 25 ° C. is preferably 10.5 or more, more preferably 10.5 to 13, more preferably 10.5 to 12.5, and still more. Preferably it is 11-12. Although the cleaning composition for an endoscope cleaning machine of the present invention may be used as it is, a cleaning solution prepared by diluting the cleaning composition with water is usually used for cleaning. Although a dilution rate is not limited, it is preferable to dilute normally about 50 to 1000 times. The pH at the time of washing is also important for the detergency, and the cleaning composition for an endoscope washing machine of the present invention has a pH of 9.5 or more at 25 ° C., more than 10 at a 200-fold dilution with water, Furthermore, it is desirable that it is 10.5 or more. The upper limit of the pH of the 200-fold diluted product is preferably 12 or less from the viewpoint of substrate damage.

<Cleaning method of endoscope>
The endoscope cleaning method according to the present invention includes an endoscope cleaning machine using a diluted cleaning solution obtained by diluting the enzyme-containing cleaning composition for an endoscope cleaning machine according to the present invention 50 to 1000 times with water. A method for cleaning a mirror, wherein an endoscope is cleaned with a water flow of the diluted cleaning solution. In the cleaning method of the present invention, it is preferable that a liquid part for immersing the endoscope is provided in the endoscope cleaning machine, and the water flow of the diluted cleaning liquid is supplied from above the water surface of the liquid part. When the liquid part is provided, it is preferable to circulate and use the liquid in the liquid part as the diluted cleaning liquid. In the cleaning method of the present invention, the pH of the diluted cleaning solution is preferably 9.5 or higher, more preferably 10 or higher, and still more preferably 10.5 or higher, and is preferably 12 or lower from the viewpoint of substrate damage.

  As an endoscope cleaning method using an endoscope cleaning machine, a method using water injection as disclosed in JP-A-60-220032, or an ultrasonic wave described in JP-A-11-151198 is used. There is a cleaning method. Such a cleaning method using flowing water or ultrasonic waves is used in an endoscope cleaning / disinfecting machine OER-2, OER-3, etc. manufactured by Olympus. In such a cleaning machine, cleaning is performed by ejecting cleaning liquid stored in a cleaning tank that can be immersed in the endoscope from a nozzle installed on the water surface to the endoscope surface or the cleaning tub cover at high pressure. Yes. Furthermore, the endoscope is reusable by continuously discharging the cleaning liquid, rinsing, and subsequently immersing in the disinfecting liquid. Here, installing the nozzle for ejecting the cleaning liquid on the water surface has the advantage that the spraying state of the cleaning liquid can be visually confirmed and the cover can be cleaned, while bubbles are very easily formed in the cleaning tank. There's a problem. If the cleaning liquid circulation line or nozzle is clogged, the cleaning power will decrease. If the cleaning is not enough afterwards, even if disinfection is performed, sufficient effects cannot be obtained, and the bacteria may survive and may cause nosocomial infection. It is very important that it can be confirmed visually. On the other hand, if bubbles are generated in the cleaning tank, the water level sensor installed in the cleaning tank malfunctions and the cleaning operation stops, bubbles overflow from the cleaning tank, and the cleaning power decreases. It is known to occur.

  The cleaning composition of the present invention can be used in the above-described cleaning method, but can be cleaned without causing a problem of foaming when supplied to an endoscope cleaning machine. The dilution ratio of the cleaning composition of the present invention is preferably 50 to 1000 times, more preferably 100 to 500 times, and particularly preferably 200 to 400 times from the viewpoint of detergency and cost. preferable. In addition, it is preferable that the cleaning composition is automatically supplied to the cleaning machine by setting the cleaning composition in the cleaning machine because it is possible to prevent forgetting to put in the cleaning composition. The viscosity of the diluted cleaning liquid prepared from the cleaning composition of the present invention is preferably 10,000 mPa · s or less, more preferably 1000 mPa · s, and particularly preferably 300 ppm or less. The washing temperature (temperature of the diluted washing solution) at this time is preferably 5 to 50 ° C., more preferably 10 to 40 ° C. in terms of washing power. The washing time is preferably 30 seconds to 30 minutes, more preferably 1 minute to 15 minutes.

  The enzyme-containing detergent composition for an endoscope washer shown in Table 1 was prepared, and the antifoaming property and the detergency were evaluated by the following methods. The results are shown in Table 1. In addition, pH was measured using HORIBA, Ltd. pH meter F-21.

<Foam suppression>
Evaluation was performed using an endoscope cleaning / disinfecting apparatus OER-2 manufactured by Olympus Medical Systems. Tap water cooled to 5 ° C. was supplied. The enzyme-containing cleaning composition for an endoscope cleaning machine was put into a cleaning tank simultaneously with tap water. The washing time was set to 10 minutes, and the foam state during washing was evaluated according to the following criteria.
4 There are few bubbles and there is no problem in operation.
3 There is much foaming, and the liquid level is slightly increased by the foam. There is no particular problem with cleaning. 2 Foaming is intense, the liquid level rises, and if washed for a long time, bubbles may overflow. Deterioration of cleaning performance due to foaming is observed. 1 Unusable due to significant foaming because liquid leaks from the device.
If the evaluation score is 3 or more, it can be used as an enzyme-containing cleaning composition for an endoscope cleaning machine.

<Detergency>
A model soil described in EN / ISO15883-5 Annex R consisting of glycerin, serum, mucin, flour and safranin was applied to a Teflon test piece at a rate of 10 mg / cm ² and dried at room temperature for 1 hour. Used for experiments. The cleaning was carried out by fixing in an endoscope cleaning / disinfecting device OER-2 manufactured by Olympus Medical Systems Co., Ltd., and setting the cleaning time to 10 minutes. After washing, the test piece was taken out and rinsed gently with ion-exchanged water in a separately prepared water tank. After drying, determine whether there is any residual dirt visually, and if there is no visible residue, immerse in Coomassie Protein Assay Reagent (reagent supplied with the protein quantification kit, manufactured by Thermo Scientific) for 3 minutes ( CBB staining), and after rinsing with ion-exchanged water, the dyeing state was determined according to the following criteria. In the test, five test pieces were used, and the result was an average value.
Judgment criteria 5: Residual dirt is hardly observed even visually or after CBB staining.
4: No residue is visually observed, but protein residue is partially observed in CBB staining.
3: Although no residue is visually observed, protein residue is observed on the entire surface by CBB staining. 2: A slight residue is visually observed 1: A large amount of blood is visually observed Evaluation point 3.6 If it is above, it is a level which does not have a problem in reuse, and it is judged that it was able to wash | clean well.

Branched nonionic surfactant (1): R in the general formula (I) is a branched alkyl group having 9 carbon atoms, m is 9, n is 5.2, and EO and PO are random adducts. Ionic surfactant (Plurafac LF901 (manufactured by BASF Japan))
Branched nonionic surfactant (2): R in the general formula (I) is a branched alkyl group having 9 carbon atoms, m is 5.8, n is 4.8, and EO and PO are random adducts. Certain nonionic surfactant (Plurafac LF900 (manufactured by BASF Japan))
Branched nonionic surfactant (3): When represented by the structure of the general formula (I) for convenience, R in the general formula (I) is a branched alkyl group having 12 to 14 carbon atoms, m is 7.0, Nonionic surfactant (Softanol 7085 (manufactured by Nippon Shokubai Co., Ltd.)), which is a block adduct in the order of n = 7.5, EO and PO = EO, PO
Branched nonionic surfactant (4): When represented by the structure of general formula (I) for convenience, R in general formula (I) is a branched alkyl group having 8 carbon atoms, m is 3, and n is 0. A nonionic surfactant

-Linear nonionic surfactant (1): For convenience, when represented by the structure of the general formula (I), R in the general formula (I) is a linear alkyl group having 12 carbon atoms, m is 5.7, n 3.6, EO and PO are block adducts in the order of EO and PO. Nonionic surfactant / linear nonionic surfactant (2): For convenience, the structure of the general formula (I) R in general formula (I) is a linear alkyl group having 8 carbon atoms, m is 0, and n is 3.0. Nonionic surfactant / linear nonionic surfactant (3): General formula for convenience In the structure of (I), R in the general formula (I) is a linear alkyl group having 8 carbon atoms, m is 3.0, n is 2.5, EO and PO are EO, and PO in this order. Nonionic surfactant / linear nonionic surfactant as an adduct (4): When represented by the structure of general formula (I) for the sake of convenience, R in general formula (I) is a straight chain having 8 carbon atoms. Nonionic surfactant / linear nonionic surfactant (5): R ′ which is an alkyl group, m is 7.0, n is 10.0, EO and PO are block adducts in the order of EO and PO A nonionic surfactant represented by —O— (EO) _m1 — (PO) _n1 — (EO) _m2 —H, wherein R ′ is a linear alkyl group having 8 carbon atoms, and m1 is 3.5, n1 is 2.5, m2 is 3.5, EO, PO, and EO are non-ionic surfactants in this order.

Sabinase: Novozyme, protease, enzyme activity 12 PU / g

Claims (8)

An enzyme-containing cleaning composition for an endoscope cleaning machine, which contains (A) a surfactant in an amount of 1 to 50% by mass and (B) a protease, and contains the following general formula (I The enzyme-containing cleaning composition for an endoscope cleaning machine, wherein the ratio of the nonionic surfactant represented by) is 90% by mass or more.
R—O — [(EO) _m / (PO) _n ] —H (I)
[Wherein, R is a branched alkyl group having 7 to 9 carbon atoms, EO is an ethanediyloxy group, PO is a propanediyloxy group, m and n are average added mole numbers, m is a number of 1 to 30, n is a number from 2 to 50. “/” Is a symbol indicating that EO and PO may be random or block. The order of adding EO and PO is not limited. ] The enzyme-containing cleaning composition for an endoscope cleaning machine according to claim 1, comprising 5 to 80% by mass of a polyhydric alcohol. The enzyme-containing cleaning composition for an endoscope cleaning machine according to claim 1 or 2, which has 1 to 30% by mass of an alkaline agent. The enzyme-containing cleaning composition for an endoscope cleaning machine according to claim 3, wherein the alkaline agent is monoethanolamine. Cleaning an endoscope with an endoscope cleaner using a diluted cleaning solution obtained by diluting the enzyme-containing cleaning composition for an endoscope cleaner according to any one of claims 1 to 4 with water by 50 to 1000 times. A cleaning method for cleaning an endoscope with a water flow of the diluted cleaning solution. The cleaning method according to claim 5, wherein a liquid part for immersing the endoscope is provided inside the endoscope cleaning machine, and a water flow of the diluted cleaning liquid is supplied from above the water surface of the liquid part. The cleaning method according to claim 5 or 6, wherein the diluted cleaning solution has a pH of 9.5 or more. The cleaning method according to claim 6 or 7, wherein the liquid in the liquid part is circulated and used as the diluted cleaning liquid.