JP2012034699A - 汎用収集用保存液 - Google Patents
汎用収集用保存液 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
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| US59857100A | 2000-06-21 | 2000-06-21 | |
| US09/598,571 | 2000-06-21 |
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| JP2002504690A Division JP4879447B6 (ja) | 2000-06-21 | 2001-06-21 | 汎用収集用保存液 |
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Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CA2313641A1 (en) * | 1997-12-12 | 1999-06-24 | Digene Corporation | Universal collection medium |
| US20040115692A1 (en) * | 2000-04-03 | 2004-06-17 | Cytyc Corporation | Methods, compositions and apparatuses for detecting a target in a preservative solution |
| US7439016B1 (en) * | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| CA2445204C (en) | 2002-10-16 | 2014-08-12 | Streck Laboratories, Inc. | Method and device for collecting and preserving cells for analysis |
| EP1605244A1 (en) * | 2004-06-09 | 2005-12-14 | Boon, Mathilde Elisabeth | Fixative composition |
| WO2009066695A1 (ja) | 2007-11-20 | 2009-05-28 | Olympus Corporation | 糞便試料の調製方法、糞便試料調製用溶液、及び採便用キット |
| JP2011518333A (ja) * | 2008-04-17 | 2011-06-23 | キアジェン ゲイサーズバーグ インコーポレイテッド | 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット |
| CN102027132A (zh) | 2008-05-12 | 2011-04-20 | 奥林巴斯株式会社 | 粪便处理方法及粪便处理容器 |
| TWI419974B (zh) | 2008-10-27 | 2013-12-21 | Qiagen Gaithersburg Inc | 於自動平台上之快速結果雜交捕捉法 |
| EP2379752B1 (fr) * | 2009-01-19 | 2015-09-30 | Biomérieux S.A. | Procedes pour determiner la susceptibilite a contracter une infection nosocomiale chez un patient et pour etablir un pronostic d'evolution d'un syndrome septique |
| US11634747B2 (en) | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| US8304187B2 (en) | 2009-02-18 | 2012-11-06 | Streck, Inc. | Preservation of cell-free RNA in blood samples |
| AU2010242867B2 (en) | 2009-05-01 | 2016-05-12 | Qiagen Gaithersburg, Inc. | A non-target amplification method for detection of RNA splice-forms in a sample |
| EP2478087B1 (en) | 2009-09-14 | 2017-01-18 | QIAGEN Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
| WO2011094514A1 (en) * | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP2013517803A (ja) | 2010-01-29 | 2013-05-20 | キアジェン ゲイサーズバーグ インコーポレイテッド | サンプル中のｈｐｖの存在を決定および確認する方法 |
| WO2011146683A1 (en) * | 2010-05-19 | 2011-11-24 | Metabolon, Inc. | Methods and reagents for metabolomics and histology in a biological sample and a kit for the same |
| EP2572001A2 (en) | 2010-05-19 | 2013-03-27 | QIAGEN Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| WO2011157678A1 (en) | 2010-06-14 | 2011-12-22 | Qiagen Gmbh | Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples |
| US10011884B2 (en) | 2010-11-12 | 2018-07-03 | Incelldx, Inc. | Methods and systems for predicting whether a subject has a cervical intraepithelial neoplasia (CIN) lesion from a suspension sample of cervical cells |
| EP2678442B1 (en) | 2011-02-24 | 2019-01-16 | QIAGEN Gaithersburg, Inc. | Materials and methods for detection of hpv nucleic acid |
| EP2704740B1 (en) | 2011-05-04 | 2016-10-05 | Streck, Inc. | Inactivated swine flu virus and methods of preparing it |
| EP3024323B1 (en) | 2013-07-24 | 2022-10-19 | Streck, Inc. | Compositions and methods for stabilizing circulating tumor cells |
| CA2940692C (en) * | 2014-03-18 | 2025-07-08 | Qiagen Gmbh | STABILIZATION AND ISOLATION OF EXTRACELLULAR NUCLEIC ACIDS |
| US11168351B2 (en) | 2015-03-05 | 2021-11-09 | Streck, Inc. | Stabilization of nucleic acids in urine |
| US10365189B2 (en) * | 2015-05-07 | 2019-07-30 | Steven Wheeler | Histological specimen treatment |
| EP3329244B1 (en) | 2015-07-30 | 2022-10-05 | Qiagen GmbH | Method of preparing a frozen biological sample |
| US20170145475A1 (en) | 2015-11-20 | 2017-05-25 | Streck, Inc. | Single spin process for blood plasma separation and plasma composition including preservative |
| EP3491381B1 (en) | 2016-07-29 | 2025-09-24 | Streck LLC | Suspension composition for hematology analysis control |
| CN106929421B (zh) * | 2017-02-27 | 2020-12-01 | 中国计量科学研究院 | 定量保存微生物的冷冻干燥小球的制备方法 |
| JP7293244B2 (ja) | 2017-10-19 | 2023-06-19 | ストレック， インコーポレイテッド | 溶血、凝固調節および細胞外小胞の安定化のための組成物 |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58206965A (ja) * | 1982-02-04 | 1983-12-02 | ジエ−ムズ・エス・ハリソン | 生物学的系用保存剤および固定剤調剤 |
| JPS59222401A (ja) * | 1983-05-30 | 1984-12-14 | アルツ−ル・フアイフエル・ヴア−クウムテクニツク・ヴエツツラ−・ゲ−エムベ−ハ− | 生物学的標本の製造方法 |
| JPH0523179A (ja) * | 1991-05-01 | 1993-02-02 | Cytyc Corp | 細胞保存溶液 |
| US5370128A (en) * | 1993-12-02 | 1994-12-06 | Wainwright; Sharon R. | Pap brush and pap unit container systems |
| US5679333A (en) * | 1996-10-25 | 1997-10-21 | Dunphy; Brian William | Formaldehyde-free tissue preservative compositions |
| WO1998051693A1 (en) * | 1997-05-13 | 1998-11-19 | Genpoint As | Solid-phase nucleic acid isolation |
| WO1999031273A2 (en) * | 1997-12-12 | 1999-06-24 | Digene Corporation | Universal collection medium |
| US5976829A (en) * | 1994-04-22 | 1999-11-02 | Birnboim; Hyman C. | Dual purpose tissue fixative |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US129223A (en) * | 1872-07-16 | Improvement in privy-seats | ||
| US133972A (en) * | 1872-12-17 | Improvement in bolt-trimmers | ||
| US4578282A (en) * | 1982-02-04 | 1986-03-25 | Harrison James S | Composition for diagnostic reagents |
| US4465078A (en) * | 1982-09-30 | 1984-08-14 | Medtest Corporation | Method for cell sampling in a body cavity |
| US4888278A (en) * | 1985-10-22 | 1989-12-19 | University Of Massachusetts Medical Center | In-situ hybridization to detect nucleic acid sequences in morphologically intact cells |
| DE69022246T2 (de) | 1989-12-01 | 1996-02-29 | Amoco Corp | Detektion von hpv-transkripts. |
| US5580970A (en) * | 1989-12-01 | 1996-12-03 | Amoco Corporation | Detection of HPV transcripts |
| US5543294A (en) * | 1991-07-19 | 1996-08-06 | The Trustees Of Columbia University In The City Of New York | Polymerase chain reaction/restriction fragment length polymorphism method for the detection and typing of myobacteria |
| IL106273A0 (en) | 1992-07-17 | 1993-11-15 | Res Dev Foundation | Rapid detection of biopolymers in stained specimens |
| US5357977A (en) * | 1993-04-23 | 1994-10-25 | St. Mary's Hospital And Medical Center, Inc. | Cytological sampling method and device |
-
2001
- 2001-06-21 AU AU1548802A patent/AU1548802A/xx active Pending
- 2001-06-21 BR BR0112385-8A patent/BR0112385A/pt not_active IP Right Cessation
- 2001-06-21 DE DE60142048T patent/DE60142048D1/de not_active Expired - Lifetime
- 2001-06-21 AT AT01984035T patent/ATE466930T1/de not_active IP Right Cessation
- 2001-06-21 AU AU2002215488A patent/AU2002215488B8/en not_active Ceased
- 2001-06-21 EP EP01984035A patent/EP1294853B1/en not_active Expired - Lifetime
- 2001-06-21 WO PCT/US2001/041091 patent/WO2001098542A2/en not_active Ceased
- 2001-06-21 CA CA002414102A patent/CA2414102A1/en not_active Abandoned
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2003
- 2003-01-24 US US10/351,149 patent/US7371518B2/en not_active Expired - Fee Related
-
2007
- 2007-07-19 AU AU2007203372A patent/AU2007203372B2/en not_active Ceased
-
2011
- 2011-09-30 JP JP2011216080A patent/JP2012034699A/ja active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58206965A (ja) * | 1982-02-04 | 1983-12-02 | ジエ−ムズ・エス・ハリソン | 生物学的系用保存剤および固定剤調剤 |
| JPS59222401A (ja) * | 1983-05-30 | 1984-12-14 | アルツ−ル・フアイフエル・ヴア−クウムテクニツク・ヴエツツラ−・ゲ−エムベ−ハ− | 生物学的標本の製造方法 |
| JPH0523179A (ja) * | 1991-05-01 | 1993-02-02 | Cytyc Corp | 細胞保存溶液 |
| US5370128A (en) * | 1993-12-02 | 1994-12-06 | Wainwright; Sharon R. | Pap brush and pap unit container systems |
| US5976829A (en) * | 1994-04-22 | 1999-11-02 | Birnboim; Hyman C. | Dual purpose tissue fixative |
| US5679333A (en) * | 1996-10-25 | 1997-10-21 | Dunphy; Brian William | Formaldehyde-free tissue preservative compositions |
| WO1998051693A1 (en) * | 1997-05-13 | 1998-11-19 | Genpoint As | Solid-phase nucleic acid isolation |
| WO1999031273A2 (en) * | 1997-12-12 | 1999-06-24 | Digene Corporation | Universal collection medium |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002215488B2 (en) | 2007-04-19 |
| DE60142048D1 (de) | 2010-06-17 |
| US20030119049A1 (en) | 2003-06-26 |
| WO2001098542A3 (en) | 2002-06-20 |
| JP4879447B2 (ja) | 2012-02-22 |
| EP1294853B1 (en) | 2010-05-05 |
| BR0112385A (pt) | 2003-06-17 |
| CA2414102A1 (en) | 2001-12-27 |
| ATE466930T1 (de) | 2010-05-15 |
| JP2004500897A (ja) | 2004-01-15 |
| AU1548802A (en) | 2002-01-02 |
| US7371518B2 (en) | 2008-05-13 |
| AU2002215488B8 (en) | 2007-11-15 |
| AU2007203372A1 (en) | 2007-08-09 |
| AU2007203372B2 (en) | 2011-02-03 |
| WO2001098542A2 (en) | 2001-12-27 |
| EP1294853A2 (en) | 2003-03-26 |
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