JP2011190258A - Sedum dendroideum ssp. praealtum (sedan grass) extract - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide new cosmetics, health food materials and medical materials from plant ingredients, comprising natural product-original ingredients as active ingredients, having no fear on safety, exerting excellent effects on rough skin improvement, skin antiaging and health foods, and kind to the skin by the effects. <P>SOLUTION: The ingredients extracted with a solvent from leaves and stems of Sedum dendroideum ssp. praealtum (sedan grass) exhibit in the body, effectiveness on cosmetics, medical materials and health food materials. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

The present invention relates to cosmetics and health foods, and more particularly, improves the rough skin, prevents aging of the skin, has excellent hygiene effects, and has excellent effects of stabilization. Sedan dendroideum ssp. praealtum), also known as sedan grass or a vegetable cosmetic, health food and medical material containing an extract as an active ingredient.

Conventionally, various cosmetics such as emulsions, creams, lotions, packs, cleaning agents, ointments and the like have been used for the purpose of improving rough skin and preventing skin aging. As the medicinal ingredients of such cosmetics, a wide variety of medicinal ingredients such as synthetic products, extracts from natural products, derivatives thereof and the like have been proposed, and many natural products such as aloe, It has been proposed that an extract from dokudomi and wormwood is effective as an external medical agent (see Patent Document 1).

There are not enough medicinal herbs, sesame, forsythia, and extracts from these natural products as medical materials for anti-allergic prevention, etc., and the effectiveness of medicinal components from natural products is ineffective. There is a drawback that it is sufficient or there are variations in its effect (see Patent Document 2).

As these natural plants, sesame, pepper, dokudami, bakumondo and the like are described as medical materials relating to the skin (see Patent Document 3).
There are many types and genera in the genus, such as Aeonium spp., Crassula spp. In particular, there is a description that there are many medicinal effects for the Red Scenery genus plants, and there are many patent documents (see Patent Documents 3 to 6).

  In the genus Craxaceae, this is extracted with water, methyl alcohol, ethyl alcohol, etc., and is proposed for dermatological surgery agents, skin prevention agents, emulsions, creams, lotions, cosmetics, cleaning products, cosmetics, etc. (See Patent Document 4).

The plant of the genus Astragalus is extracted with water, alcohol or the like, and has been proposed for external preparations, cosmetics, shampoos, rinses, bathing agents, and foods and beverages (see Patent Document 5).
Furthermore, Ryukyu benkei plants belonging to the genus Craxaceae are extracted with water, alcohol, etc., and used for cosmetics, soaps, body shampoos, skin aging prevention, foods and drinks, etc. (see Patent Document 6).

However, there is no description about the medicinal effect on sedan dendribin (sedan grass) belonging to the genus Sedum genus, and the detailed medicinal effect of natural crassulaceae sedan dendrivin is hardly known.
Sedan grass, which is a succulent plant of the genus Sedum, differs from each of the other genus Pseudoaceae in terms of active ingredient or efficacy, and sedan grass is a new and effective plant having specific active ingredients and efficacy.

JP 2000-119155 A Japanese Patent Laid-Open No. 11-180885 JP-A-10-45615 JP 2004-210653 A JP 2002-020301 A JP 2005-029483 A

The problem to be solved by the present invention is a natural product or an extract, which is excellent in the effect of medicinal ingredients for improving rough skin and preventing skin aging and the effect excellent in health food, and has no variation in the effect , Providing health food and medical materials.

Moreover, the water containing the active ingredient of a diatomaceae sedan dendribin (sedan grass) extract is obtained.

In particular, it is a valuable extract collected from the sedum dendrobin (sedan grass), which contains life-active substances. This component has a universal effect and is generally said to have effects such as disease treatment, metabolic activity, bactericidal effect, and stabilizing effect.

Application of this ingredient to cosmetics, health foods, and medical materials has not been promoted. The objective of this study was to incorporate the active ingredients of this diatomaceous sedan dendribin (sedan grass) into cosmetics, health foods, and medical materials that are effective as daily necessities.

As a result of studying the present invention to solve the above-mentioned problems, the extract of Sedum dendroideum ssp. Praelutum as a main active ingredient is used as a main active ingredient for safety, improvement of rough skin, It can be provided as a raw material for anti-aging cosmetics. As the main active ingredient, it can be provided as a raw material for medical materials for physical hygiene and as a raw material for health materials that can exert excellent effects on health foods.

It is an extract of crassulaceae sedan dendribin (sedans grass) containing components obtained by crushing crassulaceae sedan dendrivin (sedans grass) and extracted with a solvent.
The leaves and stems of the crassulaceae sedan dendribin (sedan grass) are crushed and extracted with alcohols or water at a temperature of 20 ° C. to 60 ° C., and the distillate is obtained.
As a main active ingredient, diatomaceous sedan dendribin (sedan grass) can be provided as a raw material for cosmetics effective in improving rough skin and preventing skin aging.

As the main active ingredient, a diatomaceous sedan dendribin (sedan grass) extract can be provided as a raw material for medical materials for body hygiene.
As the main active ingredient, a diatomaceous sedan dendribin (sedan grass) or an extract can be provided as a raw material for health food materials.

  A diatomaceous sedan dendribin (sedan grass) has bactericidal and antibacterial effects. In addition, cosmetic emulsions, lotions, facial cleansers, shampoos, rinses and bar soaps are used as raw materials for cosmetics effective in improving rough skin and preventing skin aging. The raw materials for health food materials are healthy juice drinks and jelly-like health confectionery.

  Any humectant can be applied as the humectant used in the cosmetic according to the present invention. Examples thereof include hyaluronic acid, collagen, glycine, alanine, valine and isoleucine. These can also be added. Furthermore, the extract of the diatomaceous sedan dendribin (sedan grass) has a good compatibility with animal fats such as horse oil and becomes a novel cosmetic.

  In the cosmetic according to the present invention, other components that are usually used as cosmetic ingredients can be applied within a range that does not impair the effect. This includes, for example, oils, surfactants, metal soaps, gelling agents, powders, alcohols, water-soluble polymers, etc. in addition to water such as purified water, hot spring water, and deep layer water.

The types of cosmetics according to the present invention include basic cosmetics such as emulsions, creams, lotions, packs, oils, facial cleansers and whole body cleansers, toothpastes, shampoos, rinses, hair treatments, hair creams, hair sprays, hair Tonics, hair preparations, hair cosmetics such as hair growth and nourishing agents, makeup cosmetics such as foundations, white powder, lipsticks, and beauty nails, anti-odor agents, acne antiseptics, ointments and the like. Examples of the form include solid, paste, mousse, gel, powder, solution, solubilization, emulsification, powder dispersion, and multilayer.

The types of food include juice, jelly, yogurt, ice cream, soup, bread, cake, and cookies.
Add sedan extract (0.05% to 20%) to each food to make it a health food.

In the present invention described above, a natural product, Sedum dendroideum ssp. Praelutum, which is a natural product, is used as an active ingredient, and thus there is no concern about safety, improvement of rough skin and prevention of skin aging. In addition, there is an effect that it is possible to provide cosmetics, medical materials, and health materials that exhibit excellent effects on skin symptoms and the like and that do not vary in their effects. The prescription described in the following symptoms is effective.

1) Putting sedan grass extract as a bathing agent into a bathtub and bathing brought about mental stability due to volatile components, and further activated the skin due to sedan grass components.
2) By applying sedan grass extract to the face before going to bed, acne and pimples were reduced.
3) Drop 1 or 2 drops on the insole of the shoe, rubbing between the toes to prevent the foot from smelling. It was effective against athlete's foot.

4) In case of burns, after cooling the affected area, applying sedan grass extract accelerated recovery.
5) To prevent bad breath, rinsing gargle and mouth with a few drops of sedan grass extract in water eliminated the germs in the mouth and eliminated bad breath.

6) Inclusion of a sedan grass extract in a cotton swab etc. and rubbing into the affected area before going to bed reduced dental caries, alveolar pyorrhea, stomatitis and periodontal disease.
7) As allergic rhinitis and hay fever, it was effective when contained in a mask and inhaled.

8) It is effective in eliminating Staphylococcus aureus by applying to the skin of a person with atopic dermatitis.
9) As an insect bite, when I rub it into the bite with a cotton swab, the pain is soft.
10) Injecting sedan grass extract into foods or pet foods showed antiseptic and antibacterial effects, and the foods lasted longer. It could also be used as a sterilizing spray as an antibacterial liquid.
11) It is effective for internal diseases by ingesting as drinking water (such as juice).

As for the effect, all the extracts of the crassulaceae sedan dendribin (sedan grass) of the present invention exert the desired effects on the improvement of rough skin and the prevention of skin aging. The extract of grass) exerted the desired effect on the disinfecting health material.

Photo of crassulaceae sedan dendribin (sedan grass) Figure of antibacterial test results of sedan dendribin A: Escherichia coli B: Staphylococcus aureus C: General bacteria Figure of SOD-like activity test results of sedan dendribin Pastel cleanser with crassulaceae sedan dendribin (sedan grass) Toothpaste with crassulaceae sedan dendribin (sedan grass)

The following points are mentioned as embodiments according to the present invention.

The stem and leaf portions of 300 g of sedan dendribin (sedan grass) in FIG. 1 were cut to about 3 cm and placed in a 3 l Erlenmeyer flask. To this, 2500 ml of 90% ethanol aqueous solution was added and sealed, and then allowed to stand at room temperature for 14 days.

The above solution was filtered to collect the filtrate, which was heated and concentrated. In the concentration by evaporating to dryness at 60 ° C. for 12 hours, ethanol was completely removed, water was evaporated as much as possible, and the remaining residue was weighed. When the water could not evaporate, the concentration was stopped at that time in consideration of decomposition of the components.

As a result, since the extract could not be completely dried, the concentration was stopped while water remained, and as a result, 231.7 g of a suspension was recovered. As a result of confirming the concentration, the extract was 5.1 g, which was 2.2%.
Therefore, the following test examples were carried out using this aqueous solution (this solution was used as a sedan dendrobin (sedan grass) extract). Depending on the test, this solution was diluted at any time and concentrated after use.

The tyrosinase inhibitory activity test of sedan dendribin (sedan grass) extract was performed.
It was examined whether this extract has an inhibitory effect on tyrosinase, which is one of the causes of stains and buckwheat. As test samples, there are five types of sedan dendribin (sedan grass) extract: 0.01% aqueous solution, 0.1% aqueous solution, 0.5% aqueous solution, 1.0% aqueous solution and non-contained semen solution (control). Tested with samples.

In this test, the substrate solution of doper and tyrosinase were reacted at 37 ° C. for 5 minutes, and the absorbance was measured at 475 nm, which is the absorption wavelength of the reaction product of doperchrome, and the tyrosinase activity was measured therefrom. As the buffer solution, a phosphate buffer solution (0.2 M, pH 6.8) was used. A spectrophotometer UV mini 1240 (manufactured by Shimadzu Corporation) was used as a measuring apparatus.
Samples for measurement are as follows.
L-DOPA solution (2.17 mM) 0.69 ml
0.75 ml of test sample
Tyrosinase solution (1250 units / ml) 0.06 ml
1.50 ml in total
Tyrosinase activity was calculated based on the measurement result of the control (purified water) as 100% (inhibition activity was 0%), and the tyrosinase inhibitory activity of each test sample was calculated based on this.
The above results are shown in Table 1.
First, a tyrosinase inhibitory activity test is located in the whitening effectiveness test in the cosmetics field. Tyrosinase activity, which is an enzyme, is largely involved as a cause of melanin production such as spots and buckwheat. Since the activity of this tyrosinase works to produce melanin and make spots, etc., many searches for components that inhibit tyrosinase have been made in the past.

Therefore, as a result of examining whether or not the sedan dendribin (sedan grass) extract has tyrosinase inhibitory activity this time, 71% inhibitory activity was observed in a 1.0% aqueous solution.
Considering that this is a sedan dendribin (sedan grass) extract, it is considered that there are relatively many components having inhibitory activity. Although there are some components that are equivalent to or better than those currently common, it is difficult to search for crude extracts that have equivalent or better effects. It is considered to be useful for whitening because it is a sedan dendribin (sedan grass) extract solution and has an inhibitory activity of 71%.

In order to examine whether the sedan dendribin (sedan grass) extract has water retention ability, a water retention test of the extract was performed.
As test samples, a sedan dendribin (sedan grass) extract of 1.0% aqueous solution, 5.0% aqueous solution, for comparison, 1% sodium hyaluronate 0.5% aqueous solution, no semen solution (control) Four types of samples were tested.

As a test method, filter paper (No. 2 55 mm, manufactured by Toyo Filter Paper Co., Ltd.) was prepared, and one side was coated with a gum tape (in order to unify moisture evaporation in one direction). Next, before the test sample was dropped, the weight of the filter paper was precisely measured, and then 0.2 ml of the above four test samples were dropped. The weight dropped at this time was accurately measured as immediately after. Thereafter, the total weight was measured at a predetermined time, the weight of water remaining on the filter paper was calculated, and the water retention capacity was evaluated with the water content immediately after being taken as 100%. This test was performed three times for one specimen, and the average value was used for evaluation.

In addition, at the time of implementation of this test, the room temperature and humidity were constant (25 ° C., 51 to 53%), and the test was performed in a windless environment. As the equipment used, a precision electronic balance GR-200 (manufactured by A & D Co., Ltd.) was used. The results are shown in Table 2.

Regarding the moisture retention test, no remarkable results were observed in the 1.0% aqueous solution and the 5.0% aqueous solution. From this, it is estimated that the sedan dendribin (sedan grass) extract is not the one with the high water retention ability. This time, we conducted a test using filter paper. This is a test that considers the safety before using human skin, and at the same time, because of the high correlation of human skin, extracts that have not been confirmed to be safe Etc. is a convenient test.

As a result, even if the sedan dendribin (sedan grass) extract does not have water retention, an increase in water retention is observed by increasing the concentration from 1.0% to 5.0%. Therefore, sedan dendribin (sedan grass) extract is likely to contain some moisturizing active ingredients, and there is a possibility that a synergistic effect can be obtained by mixing with existing moisturizing ingredients. Cannot be denied.

In the antibacterial test, the effect of sedan dendrobin (sedan grass) extract on Escherichia coli, Staphylococcus aureus and general bacteria was examined.
As test samples, three types of samples, a 1.0% aqueous solution and a 5.0% aqueous solution of sedan dendrobin (sedan grass) extract, and a physiological saline solution (control) for comparison were tested. Three types of bacterial cells were used as target bacteria: Escherichia coli, Staphylococcus aureus, and general bacteria.

As a test method, first, various types of bacteria were pre-cultured, and a bacterial solution was prepared using this. Test samples were prepared so as to have final concentrations of 1 and 5% after addition of the bacterial solution, as many as the number of target bacteria, and each bacterial solution was added to the test sample so that the final concentration was 104 to 106 CFU / ml. Thereafter, shaking culture was started at 37 ° C., and the number of bacteria was measured at a predetermined time.

For the measurement of the number of bacteria when acting for a predetermined time, a bacterial solution diluted in a 10-fold dilution series using physiological saline was inoculated by a pour method using an SCD agar medium. After culturing at 0 ° C., colonies were counted and the number of bacteria was measured. The results are shown in FIG.

In the antibacterial test, the effect on Escherichia coli, Staphylococcus aureus and general bacteria was examined. As a result, Escherichia coli and Staphylococcus aureus were found to be extremely effective against Escherichia coli and Staphylococcus aureus since no bacteria were observed in 1.0% and 5.0% aqueous solutions after 24 hours. It was. Considering that it was not observed after 24 hours, death in a shorter time was estimated, suggesting the possibility of obtaining sterilization effects.

On the other hand, in general bacteria, although the death after 24 hours was not observed in a 1.0% aqueous solution, it decreased after 48 hours. Moreover, in the 5.0% aqueous solution, the survival of the bacteria was confirmed very slightly after 24 hours, but considering that no bacteria were observed after 48 hours, the sedan dendribin (sedan grass) extract was Although it does not have a significant effect on general bacteria, it is considered to have sufficient antibacterial effect depending on continuous use, and it works to promote those effects by combining with existing antibacterial ingredients and materials. It was thought that there was.

The SOD-like activity test of sedan dendribin (sedan grass) extract was performed.
The test samples consisted of five kinds of sedan dendribin (sedan grass) extract: 0.01% aqueous solution, 0.1% aqueous solution, 0.5% aqueous solution, 1.0% aqueous solution, and no semen solution (control). Tested with samples.

The test method is performed using a superoxide dismutase kit “SOD Test Wako” manufactured by Wako Pure Chemical Industries, Ltd. as a use kit and a spectrophotometer UV mini 1240 (manufactured by Shimadzu Corporation) as a device used. It was. Since this test used a measurement kit, the test was performed according to the instructions.

That is, various reagents were prepared, a test sample, a color developing reagent solution and an enzyme solution were added, and then reacted at 37 ° C. for 20 minutes. Next, the reaction stop solution was added, and the absorbance was measured at a wavelength of 560 nm, thereby calculating the SOD-like activity value (the control used purified water according to the instructions). In addition, the test sample adjusted to the predetermined density | concentration with purified water was used for the test. The results are shown in FIG.

As a result of measuring the SOD-like activity, a relatively good result was obtained. It is well known that humans live in need of oxygen, but this oxygen changes into the form of active oxygen in the body, leading to damage to various organs in the body, the production of oxidants, and hence the promotion of aging. Yes. Oxidation is also involved in stress and fatigue. Antioxidants are present in the body, and a typical example is superoxide dismutase, that is, SOD. Oxidation in the body of SOD suppresses oxidants, but SOD also decreases with age, so it is necessary to supplement components that act like SOD, so-called SOD-like active ingredients .

For these reasons, the search and development of SOD-like active ingredients are desired in modern times. Here, sedan dendribin (sedan grass) extract 1.0% aqueous solution showed a relatively high activity of 52.0%. It is significantly higher than the reference values shown in the literature, which are almost equivalent to the SOD-like activity of a 0.01% aqueous solution of sedan dendribin (sedan grass) extract.

The sedan dendribin (sedan grass) extract has a high SOD-like activity, and is considered to be extremely useful in the cosmetics and health food fields.

An effective cream was prepared for people with atopic dermatitis.
The skin of a person with atopic dermatitis is said to be the cause of allergies due to adherence of Staphylococcus aureus. People with atopic dermatitis were asked to apply a cream of 1.0% extract of sedan dendribin (Sedan grass) for 1 week.

As a result, the following points became clear.
1. All of them had no onset immediately after onset, onset of irritation, skin irritation, or itching.
2. All patients were swollen one day after onset, but application was started. Application was performed at intervals of 6 hours. It suppressed itching without amplifying the swelling.
3. Itching and swelling were relieved and the skin began to dry.
4. There are individual differences, but it has not been completely cured in a week, but itching has disappeared and swelling has subsided. By continuing in the future, it was gradually cured.
As for the effect, all the extracts of the crassulaceae sedan dendribin (sedan grass) of the present invention exhibited the desired effects for improving rough skin and preventing skin aging.

A 10% allergic person was allowed to eat and drink for 1 week by using a 1% aqueous solution of juice of the extract of crassulaceae sedan dendribin (sedan grass).
The two people did not change much.
Eight people gradually relieved itching, and sneezing and runny nose.
It seems to be improved by continuing drinking in the future.

Add purified water into a glass bottle or heat-resistant cup, add caustic soda a little at a time, mix the oil and caustic soda water temperatures, add oil, collagen and sedan extract, and keep it well ventilated for 2-3 months. I made a laid bar soap.
The extract of sedan grass was used as a soap for face or body because of its high bactericidal effect to kill S. aureus, inhibitory effect of tyrosinase (whitening effect), and high SOD-like activity.

In order to clean the face, moisturizing was required, so collagen was added, and a synthetic soap base was not added, and 10 people used a 1% sedan grass extract facial cleanser for 20 days. Even if there was no foam, it was said that after washing it was moist and clean.
The three clients felt tension on their skin. For 7 people, the skin became tense and the spots were thinner, the warts were smaller, and wrinkles were reduced. The atopy was not itchy while washing with soap.

To make it easier to use, make gel water with "Xanthan Gum" using "Xanthan Gum" (trade name), dissolve the soap base in it, and keep the resulting liquid soap base in a sealed bottle container etc. for one day. So, the alkalinity decreased naturally, and it changed to a state where it could be used on the skin, and oil was added to make a shampoo. Five people who became ugly using a commercially available shampoo used it.

A shampoo was made by adding some vegetable soap base to make it foam. There is not much foam, but all of them don't get scalp after use. Dandruff has gradually decreased. There was no particular change in one person, but it was refreshing after use.

A lotion was prepared by adding 20% sedan grass extract to purified water and glycerin. After the face wash, 10 people applied the lotion to be applied.
Nine people became skin tight, the spots and wrinkles lightened, and the itch disappeared. One person did not change much, but the grudge disappeared.

In order to prevent skin roughness and aging from the inside of the body, 10% sedan grass extract was added to a melted mixture of gelatin and water to prepare a healthy jelly.
Sedan grass jelly, like aloe, expressed its impression that it was easy to eat without bitterness. It was said that constipation was also resolved.

The layers, citric acid, and 10% sedan grass extract were mixed well, and water was added and solidified to the extent that the moisture would come out.
The bath was used for a week after bathing, but the rough skin was alleviated and the volatile components stabilized the spirit.

A person who had acne or pimples had a sedan grass extract diluted with water.
Acne and pimples were reduced, and skin condition improved.

Five people who smelled their sedan grass extract with water and smelled their feet were asked to leave when they came home every day. The three people did not smell any more, and the smell was decreasing. The two did not change much. Also, 3 people who are athlete's foot were attached. The two began to lose itch. One person did not change much.

It was used as a preservative by diluting the sedan grass extract with water and spraying it on food. About 5 g of dog food was put in two glass containers and kept at a room temperature of 20 ° C. and a humidity of 70%.
A sprays sedan grass extract once a day for a week. Leave B as it is.
A had no mold for a week. B began to grow a little on the 4th day, and 60% of the mold grew in a week.

1. 1. Container lid 2. Face wash with sedan extract 3. Container with face wash containing sedan grass extract 4. A container with lotion containing sedan grass extract Finger-pump type container

Claims (7)

An extract of crassulaceae sedan dendribin (sedan grass), characterized by containing a component extracted from a solvent by crushing Sedum dendroideum ssp. Praealtum. Claims characterized in that the leaves and stems of crassulaceae sedan dendribin (sedan grass) are crushed, extracted with alcohols or water at a temperature of 20 ° C to 60 ° C, and the distillate is fractionated. Item 1. An extract of the crassulaceae sedan dendribin (sedan grass) according to item 1. 3. The diatomaceous sedan dendribin (sedan grass) as a main active ingredient can be provided as a raw material for cosmetics effective in improving rough skin and preventing skin aging. An extract of the crassulaceae sedan dendribin (sedan grass). 3. An extract of the crassulaceae sedan dendribin (sedan grass) can be provided as a main active ingredient in a raw material of a medical material for physical hygiene. Extract of bottle (sedan grass) ingredients. The diatomaceous sedan dendribroin (sedan) according to claim 1 or 2, characterized in that it can be used as a raw material for health food materials as a main active ingredient. Extract of grass) The main active ingredient of crassulaceae sedan dendribin (sedan grass) is beauty milk, lotion, facial cleanser, shampoo, rinse, solid soap as a raw material for cosmetics effective in improving rough skin and preventing skin aging. The extract of the crassulaceae sedan dendribin (sedan grass) according to any one of claims 1, 2, or 3. The extract of the crassulaceae sedan dendribine (sedan grass) is used as a main active ingredient, and the raw material of the health food material is a health juice drink, a jelly-like health confectionery, or An extract of the crassulaceae sedan dendribin (sedan grass) according to claim 5