JP2011032334A - Antioxidant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an excellent antioxidant containing pine bark extracts and sesamin. <P>SOLUTION: Especially excellent antioxidant action is recognized when 1-100 pts.wt. of sesamin relative to 100 pts.wt. of pine bark extracts is contained, so that the antioxidant is achieved. The antioxidant is used orally or percutaneously without modification or with various components added, as foods and drinks or cosmetics. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to an antioxidant comprising pine bark extract and sesamin, and containing 1 to 100 parts by weight of sesamin with respect to 100 parts by weight of pine bark extract.

  It is known that cells are damaged when excess active oxygen is present in the living body. As a defense mechanism against this, there are various active oxygen production suppression mechanisms and elimination mechanisms in living organisms. However, when the active oxygen production suppression mechanism or the elimination mechanism does not function sufficiently, or when the production of active oxygen increases due to factors such as aging or external stress, the active oxygen is excessively present and the excess of lipid is present. Oxidation is caused. As a result, it is known to cause various disorders and diseases.

Various antioxidants have been developed in order to prevent oxidation in vivo by active oxygen as described above. For example, fermented product obtained from garlic (Patent Document 1), fermented product obtained from bitter gourd (Patent Document 2), lactic acid fermented product of onion (Patent Document 3), fermented product containing citrus peel (Patent Document 4), professional A composition (Patent Document 5) containing anthocyanidin and at least one selected from the group consisting of astaxanthin, ubiquinone, lignans, curcumin, and curcumin derivatives is known. However, regarding the antioxidant containing pine bark extract and sesamin, it was not clear what combination ratio of pine bark extract and sesamin would be expected to have a particularly excellent antioxidant effect. It was.

JP 2006-94853 A JP 2006-75083 A JP-A-2005-97222 JP 2004-189718 A Japanese Patent Laid-Open No. 2005-21098

  An object of the present invention is to provide an antioxidant, which contains a pine bark extract and sesamin, and contains 1 to 100 parts by weight of sesamin with respect to 100 parts by weight of the pine bark extract. To do. More preferably, it relates to the antioxidant, wherein sesamin is derived from sesame oil.

  As a result of intensive studies on the antioxidant effect of a composition containing a pine bark extract and sesamin, the present inventors contain a pine bark extract and sesamin, and sesamin with respect to 100 parts by weight of the pine bark extract. The present inventors have found that a particularly excellent antioxidation effect is observed when 1 to 100 parts by weight of is contained, and have completed the present invention.

That is, the present invention relates to an antioxidant containing pine bark extract and sesamin, and containing 1 to 100 parts by weight of sesamin with respect to 100 parts by weight of pine bark extract. More preferably, it relates to the antioxidant, wherein sesamin is derived from sesame oil.

  According to the present invention, by using an antioxidant, which contains pine bark extract and sesamin, and contains 1 to 100 parts by weight of sesamin with respect to 100 parts by weight of pine bark extract, It can be used orally or transdermally as it is, or with various components added, as foods and beverages and cosmetics.

  Hereinafter, embodiments of the present invention will be described. In addition, this invention should not be limited and interpreted by description of the following embodiment, A various change is possible within the range of description in a claim.

  The pine bark extract used in the present invention contains proanthocyanidins as one of the main active ingredients. Proanthocyanidins refer to a group of compounds consisting of a condensation polymer having a degree of polymerization of 2 or more, with flavan-3-ol and / or flavan-3,4-diol as a structural unit. It is a powerful antioxidant produced by plants and is intensively contained in plant leaves, bark, fruit peel and seeds. This proanthocyanidin is a substance that cannot be produced in the human body.

  The pine bark extract contains a condensed polymer having a polymerization degree of 2 or more as proanthocyanidins, and further contains catechin and the like. In particular, proanthocyanidins containing a large amount of a condensation polymer having a low degree of polymerization are preferably used. The condensation polymer having a low polymerization degree is preferably a condensation polymer having a polymerization degree of 2 to 30 (2 to 30 mer), more preferably a condensation polymer having a polymerization degree of 2 to 10 (2 to 10 mer). Further, a condensation polymer (2 to 4 mer) having a polymerization degree of 2 to 4 is more preferable. Proanthocyanidins, which are condensation polymers having a degree of polymerization of 2 to 4 (2 to 4 mer), are particularly easily absorbed into the body. In the present specification, the polymer having a degree of polymerization of 2 to 4 is referred to as oligomeric proanthocyanidin (hereinafter referred to as “OPC”).

  Moreover, as a pine bark extract, 15% by mass, preferably 20% by mass or more, and more preferably 30% by mass of a condensation polymer having a degree of polymerization of 2 to 4 (2 to 4 mer; that is, OPC). An extract containing a pentamer or more proanthocyanidins in a proportion of 10% by mass or more, preferably 15% by mass or more is preferable.

  The pine bark extract may further contain catechins. The catechins are preferably contained in a proportion of 5% by mass or more, more preferably 10% by mass or more. Catechin can be extracted together with proanthocyanidins (OPC) by the above extraction method. Catechin is a general term for polyhydroxyflavan-3-ol. As catechins, (+)-catechin (referred to as catechin in the narrow sense), (−)-epicatechin, (+)-gallocatechin, (−)-epigallocatechin, epigallocatechin gallate, epicatechin gallate, and afzelechin Etc. are known. In addition to the above (+)-catechin, gallocatechin, afzelekin, and (+)-catechin or 3-galloyl derivatives of gallocatechin have been isolated from the pine bark extract. Catechins are known to have a carcinogenic inhibitory effect, an active oxygen and free radical scavenging effect, and an antioxidant effect. Catechins are poor in water solubility by themselves and have low physiological activity, but in the presence of OPC, catechins have the property of being activated at the same time as water solubility increases. Therefore, catechins work effectively when ingested with OPC.

  The catechins are preferably contained in the pine bark extract in an amount of 5% by mass or more. More preferably, the pine bark extract containing 20% by mass or more of OPC and 10% by mass or more of a pentamer or more of proanthocyanidins preferably contains 5% by mass or more of catechins. For example, when the catechin content of the pine bark extract is less than 5% by mass, the catechins may be added so that the content is 5% by mass or more. It is most preferable to use a pine bark extract containing 5% by mass or more of catechins, 20% by mass or more of OPC, and 10% by mass or more of proanthocyanidins of 5 or more.

Pine bark extracts used in the present invention include Pinus Martina, larch, Japanese black pine, Japanese red pine, Japanese pine, Japanese pine, Korean pine, Japanese pine, Ryukyu pine, Utsukushima, Japanese pine, white pine, Canadian Quebec region Bark extracts such as Aneda are preferably used. Among these, a bark extract of French coastal pine is preferably used.

  French coastal pine is a marine pine that grows on the Atlantic coast of southern France. The bark of French coastal pine contains organic acids as well as other physiologically active components in addition to proanthocyanidins, which are flavonoids, as main components. This main component, proanthocyanidins, is known to have a strong antioxidant action to remove active oxygen.

  The pine bark extract is obtained by extracting the pine bark with water or an organic solvent. When water is used, warm water or hot water is used. As an organic solvent used for extraction, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, ethyl methyl ketone, Organic solvents that are acceptable for the production of food or drugs such as glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible fats and oils, 1,1,1,2-tetrafluoroethane, 1,1,2-trichloroethene Preferably used. These water and organic solvents may be used alone or in combination. In particular, hot water, hydrous ethanol, hydrous propylene glycol and the like are preferably used.

  The extraction method from pine bark is not particularly limited, and for example, a warm extraction method, a supercritical fluid extraction method, or the like is used.

  The supercritical fluid extraction method is a method of performing extraction using a supercritical fluid that is a fluid that exceeds the critical point (critical temperature, critical pressure) of a gas-liquid substance. As the supercritical fluid, carbon dioxide, ethylene, propane, nitrous oxide (laughing gas) or the like is used, and carbon dioxide is preferably used.

  In the supercritical fluid extraction method, an extraction process for extracting a target component with a supercritical fluid and a separation process for separating the target component and the supercritical fluid are performed. In the separation step, any one of extraction separation by pressure change, extraction separation by temperature change, and extraction separation using an adsorbent / absorbent may be performed.

  Moreover, you may perform supercritical fluid extraction by the entrainer addition method. In this method, for example, ethanol, propanol, n-hexane, acetone, toluene, other aliphatic lower alcohols, aliphatic hydrocarbons, aromatic hydrocarbons, and ketones are added to the extraction fluid in an amount of 2 to 20 W / V. Add about% and perform supercritical fluid extraction using this fluid to dramatically increase the solubility of the target extract, such as OPC and catechins, in the extraction solvent, or enhance the selectivity of the separation. This is a method for obtaining an efficient pine bark extract.

  Since the supercritical fluid extraction method can be operated at a relatively low temperature, it can be applied to substances that are altered or decomposed at high temperatures, the advantage that the extraction fluid does not remain, and the recycling of the solvent is possible. There is an advantage that the process can be omitted and the process becomes simple.

  Extraction from pine bark may be performed by a method such as a liquid carbon dioxide batch method, a liquid carbon dioxide reflux method, or a supercritical carbon dioxide reflux method other than the above extraction method.

  For extraction from pine bark, a plurality of extraction methods may be combined. By combining a plurality of extraction methods, it becomes possible to obtain pine bark extracts having various compositions.

  The pine bark extract obtained by the above extraction is safe to be purified by ultrafiltration or a column method or a batch method using an adsorptive carrier (Diaion HP-20, Sephadex-LH20, chitin, etc.). From the viewpoint of

  The sesamin used in the present invention is not particularly limited, and usually available sesamin, a substance containing sesamin, and the like are used. Preferably, sesame oil containing sesamin is used.

  The mixing ratio of sesamin used in the present invention is 1-100 parts by weight of sesamin per 100 parts by weight of pine bark extract, more preferably 1-10 parts by weight of sesamin, most preferably sesamin. It contains in the ratio of 1-5 weight part.

  The antioxidants described in the present invention can be used orally or percutaneously as they are or as various foods and cosmetics with various components added.

  As foods and beverages, the antioxidant described in the present invention is used as it is or various nutritional components are added, and the food is suitable, for example, powder, granule, granule, liquid, paste, cream, tablet , Capsule, caplet, soft capsule, tablet, rod, plate, block, pill, solid, gel, jelly, gummy, wafer, biscuit, bowl, chewable, syrup It can be formed into a shape or stick shape and provided as a food material. Further, it may be used by adding to water, milk, soy milk, fruit juice drink, whey drink, soft drink, green juice, yogurt and the like.

  As cosmetics, for example, as a cosmetic material in the form of lotion, cosmetic cream, milky lotion, cream, pack, hair tonic, hair cream, shampoo, hair rinse, treatment, body shampoo, facial cleanser, soap, foundation, hair conditioner, etc. Can be used.

  Examples of the present invention will be described below. It should be noted that the present invention should not be construed as being limited to the following examples, and various modifications can be made within the scope of the claims.

(Preparation of test substance)
Prepare a test substance by dissolving or suspending pine bark extract (manufactured by Toyo Shinyaku Co., Ltd.) and sesamin (sesame oil containing 1.6% by weight of sesamin) in corn oil in the proportions shown in Table 1. went.

(Measurement of in vivo lipid peroxidation inhibitory effect)
Each test substance was administered by single oral gavage to 5 to 8 9-week-old male SD rats per group. The dose was 5 mL / kg. 1 mg after administration of the test substance, 9 mg of iron nitrilotriacetic acid (Fe-NTA)
It was administered intraperitoneally at a dose of Fe / kg. Blood was collected from the orbital venous plexus before and 4 hours after administration of Fe-NTA, and serum was collected. The amount of lipid peroxide (TBARS) in serum was measured, and the antioxidant action of each test substance was evaluated.

  Using the measured amount of serum TBARS, the amount of change in serum TBARS amount (ΔTBARS) was calculated from the following equation. The results are shown in Table 2. In addition, it means that the lipid peroxide in serum is suppressed, so that (DELTA) TBARS is small, and shows that antioxidant activity is high.

  From the results of Table 2, in the group (Example 1) in which the test substance containing 1 to 100 parts by weight of sesamin was administered to 100 parts by weight of the pine bark extract, ΔTBARS was compared with the control group (Comparative Example 1). The value of was extremely small. Even in the group (Example 2) in which the pine bark extract was administered alone, the value of ΔTBARS was smaller than that in Comparative Example 1, but the degree of suppression did not reach that in Example 1. On the other hand, in the group (Example 3) to which sesamin (sesame oil) was administered alone, no significant difference was observed compared to Comparative Example 1.

Therefore, when a test substance containing 1 to 100 parts by weight of sesamin is administered to 100 parts by weight of a pine bark extract, a synergistic effect that is not expected from the results when each is administered alone. It was found that lipid peroxide in serum was remarkably suppressed.

  The antioxidant described in the present invention contains pine bark extract and sesamin, and is characterized by containing 1 to 100 parts by weight of sesamin with respect to 100 parts by weight of pine bark extract. Shows the effect. The antioxidants described in the present invention can be used orally or percutaneously as they are or as various foods and cosmetics with various components added.

Claims (2)

  An antioxidant, comprising pine bark extract and sesamin, and containing 1 to 100 parts by weight of sesamin with respect to 100 parts by weight of pine bark extract.   The antioxidant according to claim 1, wherein sesamin is derived from sesame oil.