JP2011032232A - Composition for excitation inhibition or sedation use and food and drink containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new composition for excitation inhibition or sedation use. <P>SOLUTION: There are provided the composition for excitation inhibition or sedation use containing a tomato-derived substance as an active component, and foods and drinks containing the excitation inhibiting or sedating composition. The tomato-derived substance of the excitation inhibiting or sedating composition contains a non-γ-aminobutyric acid fraction, contains guanosine or adenosine, or contains cytidine or uridine and further γ-aminobutyric acid in an amount smaller than the sum of cytidine, uridine, guanosine and adenosine. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a composition for suppressing excitement or sedating.

  In recent years, foods have been used for treatment or prevention methods that can be said to be intermediate between drug therapy and general therapy. Such foods are called health foods, functional foods, health supplements or foods for specified health use. As these foods, there are those to which a substance having a sedative effect and an excitement suppressing effect is added, and the like are sold as health foods and health drinks. Theanine, L-carnitine, L-dopa, γ-aminobutyric acid (GABA) and the like are known as components having an effect on such sedative action and excitement suppressing action.

  There is a growing need for foods that have therapeutic or preventive effects, and there is always a need for products that are more effective, products that can be taken safely and for a long time, and products with less economic burden.

  Tomatoes have long been eaten by many people as a delicious and healthy food. The main nutritional components of tomato are vitamin A, vitamin B6, vitamin C, potassium, dietary fiber, iron and the like (Non-patent Document 1).

  Specifically, tomatoes have vitamin C that works effectively for cold prevention, vitamin B6 that facilitates fat metabolism, and nutrients such as potassium that drains salt in the blood and is effective in preventing high blood pressure. Is known to be abundant.

  In addition, it is known that lycopene, which is also a red pigment of tomato, has a strong antioxidant action, and has an aging inhibitory effect, a cosmetic effect that keeps skin and skin youthful, and a cancer prevention effect.

  Furthermore, tomato contains water-soluble dietary fiber pectin, which is known to have an effect of improving constipation and preventing lifestyle-related diseases (adult diseases) by promoting the function of discharging waste products and harmful substances. It has been.

  Patent Document 1 discloses that tomato juice and its serum have fatigue-reducing effects.

JP 2006-193435 A

5th Amendment Japanese Food Standard Composition Table, Ministry of Education, Culture, Sports, Science and Technology, Science and Technology Council, Resource Survey Subcommittee, 2005

  However, it is not yet known that tomato has an excitement suppressing action or a sedative action.

  The present invention has been made from such a viewpoint, and a problem to be solved by the present invention is to provide a new composition for suppressing excitement or sedation.

  The present inventors diligently studied to solve the above problems. As a result, it was found that the tomato-derived substance exhibits an excitement suppressing action and a sedative action, and the present invention has been completed.

The present invention is as follows.
[1]

A composition for suppressing or suppressing sedation comprising a tomato-derived substance as an active ingredient.
[2]

The composition for excitation suppression or sedation according to [1], wherein the tomato-derived substance is a non-γ-aminobutyric acid fraction.
[3]

The composition for stimulating or suppressing sedation according to [1] or [2], wherein the tomato-derived substance contains guanosine or adenosine.
[4]

The composition for stimulating or suppressing sedation according to [3], wherein the tomato-derived substance further comprises cytidine or uridine, and further contains γ-aminobutyric acid in an amount smaller than the total content of cytidine, uridine, guanosine and adenosine. object.
[5]

The tomato-derived substance is selected from the group consisting of aspartic acid, glutamic acid, asparagine, serine, glutamine, arginine and alanine, which contains γ-aminobutyric acid and is less in total content than the content of γ-aminobutyric acid. The composition for exciting suppression or sedation according to [3] or [4], further comprising at least one selected from the group consisting of
[6]

The composition for stimulating or suppressing sedation according to any one of [1] to [5], wherein the tomato-derived substance is obtained by adsorbing with a synthetic adsorbent.
[7]

The composition for excitation suppression or sedation according to [6], wherein the synthetic adsorbent is SP207.
[8]

  Food / beverage products containing the composition for excitation suppression or sedation in any one of [1]-[7].

  According to the present invention, a new composition for suppressing excitement or sedation can be obtained.

It is a graph which shows content of the uridine and GABA of each fraction. It is a graph showing the measurement result of the spontaneous momentum from the start of measurement to 13 hours later, where n number of groups is 10. It is a graph showing the measurement result of the spontaneous momentum from the start of measurement to 13 hours later, where n number of groups is 10.

  Hereinafter, embodiments for carrying out the present invention will be described in detail. In addition, this invention is not restrict | limited to the following embodiment, In the range of the summary, various deformation | transformation can be implemented.

[Composition for suppressing excitement or sedation containing tomato-derived substance as active ingredient]
The composition for suppressing excitement or sedating according to the present invention comprises a tomato-derived substance as an active ingredient. Here, the tomato-derived substance in the present specification refers to a liquid obtained by squeezing tomato fruit, or a liquid or powder obtained by processing this. Such liquid can be used for drinking as it is, and the tomato-derived substance can be mixed with other vegetable juice and / or fruit juice for drinking. Furthermore, a seasoning, a sour agent, various additives, etc. can be added to the said tomato origin substance as needed. The tomato-derived substance can be filled in various containers (for example, paper containers, PET containers, steel can containers, aluminum can containers, etc.) and sold in the form of a container-packed beverage. The material, shape, size, color and the like of various containers are not particularly limited.

  Examples of the tomato-derived substance include a crude extract of tomato and a fraction (liquid or powder) obtained by adsorbing a liquid extract of the crude extract with a synthetic adsorbent. Here, “crude extract” in the present specification refers to any tomato extract (liquid, powdery form) excluding a fraction obtained by adsorbing a liquid extract of the above-described crude extract with a synthetic adsorbent. ). Specific examples of the crude extract include, but are not limited to, a tomato juice or serum thereof, or a liquid crude extract powdered. Moreover, these dilutions or concentrates may be sufficient. Among the above, from the viewpoint of obtaining a high concentration of components contained in tomatoes, a fraction obtained by adsorbing a liquid extract of a crude extract with a synthetic adsorbent (liquid, powder) Is preferred. In other words, the composition for suppressing excitement or sedation according to the present invention is preferably obtained by adsorbing with a synthetic adsorbent. The state of the fraction may be a liquid or a powder obtained by processing the liquid.

  The method for obtaining the juice juice is not particularly limited, as long as the juice can be obtained by squeezing the tomato fruit. For example, a method of squeezing tomato (washed and selected as desired) using a reamer, a method of squeezing and squeezing using a hydraulic press, a roller squeezer or an inline squeezer, a pulper finisher, etc. The method of crushing and squeezing using, the method of crushing using a crusher etc., heating with a tube heater etc., performing sterilization and enzyme deactivation, and squeezing using an extractor etc. are mentioned. Furthermore, what was squeezed (squeezed) according to these methods may be applied to a juicer and / or sterilized as desired.

  The serum in the present specification means a supernatant (sap), and the method for obtaining the serum is not limited to the following, but can be obtained, for example, by centrifuging tomato juice. Further, sterilization may be performed as desired.

  The method for obtaining the concentrate is not limited to the following, and examples thereof include normal heating concentration, reduced pressure concentration, low temperature concentration, freeze concentration, and reverse osmosis concentration.

  The method for obtaining a powdered liquid crude extract as described above is not limited to the following, but includes concentrating tomato juice, serum, etc. under reduced pressure and then freeze-drying.

  As the tomato-derived substance, a product obtained through the above-described production process may be used, or a commercially available product (for example, refer to Examples described later) may be used.

  Subsequently, a fraction obtained by adsorbing a liquid extract of a crude extract with a synthetic adsorbent will be described in detail. As described above, a fraction obtained by adsorbing a liquid extract of a crude extract with a synthetic adsorbent is preferable in that a specific one of components contained in tomato can be obtained at a high concentration. Among these, a non-γ-aminobutyric acid fraction is preferable. This is because, when the component other than γ-aminobutyric acid is the main component, the amount of spontaneous exercise is significantly reduced with a smaller amount compared to the case where γ-aminobutyric acid is the main component, including the γ-aminobutyric acid fraction. This is because an excitement suppressing effect and a sedative effect can be effectively obtained. Here, the “non-γ-aminobutyric acid fraction” in the present specification means a fraction in which the content of γ-aminobutyric acid is 1/10 or less of the content of the crude extract.

  Of the specific tomato-derived substances described so far, not limited to the above-mentioned fractions, those in which the content of γ-aminobutyric acid is 1/10 or less of the content of the crude extract are preferred. This is because when the component other than γ-aminobutyric acid is the main component, the locomotor activity can be significantly reduced in a smaller amount compared to the case where γ-aminobutyric acid is the main component, and the excitement suppressing effect and sedation can be reduced. This is because the effect can be obtained effectively.

  Here, tomato-derived substances such as the above-described non-γ-aminobutyric acid fraction will be described in terms of components. From the viewpoint of reducing the amount of spontaneous exercise and exerting an excitement suppressing action and a sedative action, the tomato-derived substance preferably contains guanosine or adenosine. In addition, it is more preferable that the tomato-derived substance further includes cytidine or uridine, and further includes γ-aminobutyric acid in an amount smaller than the total content of cytidine, uridine, guanosine and adenosine. In other words, in the tomato-derived substance, the total content of cytidine, uridine, guanosine and adenosine is more preferably larger than the content of γ-aminobutyric acid on a mass basis. The tomato-derived substance contains guanosine or adenosine, and also contains γ-aminobutyric acid, and the total content is smaller than the content of γ-aminobutyric acid. Aspartic acid, glutamic acid, asparagine, serine It is more preferable to further include one or more selected from the group consisting of glutamine, arginine and alanine. In other words, in the tomato-derived substance, the content of γ-aminobutyric acid is more preferably larger than the total content of aspartic acid, glutamic acid, asparagine, serine, glutamine, arginine and alanine on a mass basis. More preferably, it is a tomato-derived substance that satisfies both of the above two more preferable conditions.

  The tomato synthetic adsorbent adsorbed fraction (hereinafter also simply referred to as “adsorbed fraction”) is obtained by, for example, subjecting tomato-derived substances (crude extract) such as juice to be adsorbed with a synthetic adsorbent, It can be obtained by elution treatment with an elution solvent. On the other hand, the non-adsorbed fraction of tomato synthetic adsorbent (hereinafter also simply referred to as “non-adsorbed fraction”) does not adsorb when, for example, a tomato-derived substance (crude extract) is adsorbed with a synthetic adsorbent. It can be obtained by collecting the fraction that flows out into

  The method of performing the adsorption treatment by adsorbing with the synthetic adsorbent can be performed according to a conventionally known method, and is not particularly limited. Examples of the adsorption method include the following methods.

  After squeezing juice and its serum with a synthetic adsorbent, passing water through the synthetic adsorbent and pushing the water, for example, by passing 60% ethanol and collecting it, synthetic adsorption of the target tomato An agent adsorption fraction can be obtained.

  The treatment with the synthetic adsorbent may be performed by either a batch method or a column method, but a column method capable of efficiently treating with a relatively small amount of the synthetic adsorbent is preferable. The treatment with the synthetic adsorbent may be performed at least once. The synthetic adsorbent is preferably a porous adsorbent.

The specific surface area (by the BET method) of the synthetic adsorbent is preferably 100 to 1,200 m ² / g, more preferably 250 to 900 m ² / g.

  The pore volume, particle size distribution, and most frequent radius of the synthetic adsorbent are each 0.9 to 1.6 mL / g, 250 μm or more is 90% or more, and preferably 30 to 260 mm.

  Examples of the synthetic adsorbent include, but are not limited to, a hydrophilic synthetic adsorbent and a hydrophobic synthetic adsorbent, and a hydrophobic synthetic adsorbent is preferable.

  The resin matrix of the hydrophilic synthetic adsorbent is not limited to the following, and examples thereof include styrene macroporous, silica, and methacrylic acid ester polymers. Specific examples of the hydrophilic synthetic adsorbent include, but are not limited to, Diaion (registered trademark; Mitsubishi Chemical Corporation), and Muromac (registered trademark; Muromachi Technos Co., Ltd.).

Examples of the resin matrix of the hydrophobic synthetic adsorbent include, but are not limited to, styrene polymer, styrene-divinylbenzene copolymer, styrene-acrylic acid amide copolymer, and phenol resin. The hydrophobic synthetic adsorbent is not limited to the following, but is preferably a porous modified polystyrene synthetic adsorbent obtained by chemically modifying and bonding a polar group such as bromine to an aromatic resin matrix such as styrene. Examples of the hydrophobic synthetic adsorbent include, but are not limited to, for example, Sepabeads (registered trademark; Mitsubishi Chemical Corporation). Among them, Sepabeads SP70, SP700, SP850, and SP207 are preferable, and SP207 (ratio) is more preferable. The surface area is 590 m ² / g, the pore volume is 1.1 mL / g, the particle size distribution is 250 μm or more, 90% or more, and the most frequent radius is 120 mm.

  As shown in the Examples below, when the above-described SP207 is used as a synthetic adsorbent, the present inventors have found that both the synthetic adsorbent non-adsorbed fraction and the synthetic adsorbent adsorbed fraction of tomato have an excitatory inhibitory action. It was found to have a sedative effect. In other words, when the synthetic adsorbent is SP207, the γ-aminobutyric acid fraction and the non-γ-aminobutyric acid fraction are preferably used in the form of a synthetic adsorbent non-adsorbed fraction and a synthetic adsorbent adsorbed fraction, respectively. Can be separated. Moreover, said non-gamma-aminobutyric acid fraction contained guanosine or adenosine.

  The synthetic adsorbent may be pretreated prior to the adsorption treatment. Such pretreatment is not limited to the following, but, for example, the synthetic adsorbent is washed with a solvent such as methanol or ethanol to remove impurities, and further washed with water to remove the solvent such as methanol or ethanol. Is mentioned. Ethanol is preferably used for washing the synthetic adsorbent.

  The ratio between the synthetic adsorbent and the tomato-derived substance (crude extract) is selected according to the type of adsorbent used.

  As the solvent used for the elution treatment, an elution solvent suitable for the synthetic adsorbent to be used can be appropriately selected. For example, when an aromatic synthetic adsorbent is used, a large amount of water or warm water may be used as the elution solvent. From the viewpoint of increasing elution efficiency, ethanol diluted with water to a concentration of preferably 50 to 70%, more preferably 55 to 65% may be used.

  More specifically, after passing a tomato-derived substance (crude extract) through a column packed with a synthetic adsorbent, the column discharge liquid is Brix 0.5% or less, preferably Brix 0.2% or less as measured by Brix. The adsorbed fraction may be recovered by washing with pure water and elution using an elution solvent such as ethanol having an appropriate concentration.

  For example, in the case of a 60% ethanol solution, the adsorption fraction is collected by passing 1 to 10 times the resin volume, more preferably 1 to 5 times, and even more preferably 1 to 2.5 times. Adsorbed material can be collected efficiently. In addition, when water is used, the adsorbate can be recovered by washing the tomato-derived substance remaining in the resin with pure water and then passing the liquid at least three times the resin capacity.

  In the adsorption treatment, if necessary, centrifugation or filtration may be performed in advance. For example, the sample is centrifuged under conditions of 3,000 rpm, 25 ° C. and 10 minutes. Suction filtration can be performed using 2 filter papers. The fraction obtained by the adsorption treatment (tomato synthetic adsorbent adsorbed fraction or synthetic adsorbent non-adsorbed fraction) may be further purified, for example by cation exchange column chromatography or gel filtration chromatography. It can be carried out. The fractions collected by the adsorption process (synthetic adsorbent adsorbed fraction and synthetic adsorbent non-adsorbed fraction) may be concentrated under reduced pressure to an appropriate concentration, or may be freeze-dried as a powder. Good.

  In addition, the composition for suppressing excitement or sedation containing a tomato-derived substance as an active ingredient according to the present invention may be the tomato-derived substance itself, and in addition to the tomato-derived substance, for foods and drinks and for pharmaceuticals Other optional components that are usually used for the purpose (for example, seasonings, acidulants and various additives) may be included.

  The composition for stimulating or suppressing sedation containing the tomato-derived substance as an active ingredient is a solution, a concentrate obtained by concentrating the solution under reduced pressure, or a powder obtained by spray-drying, drum-drying, freeze-drying, or the like. Can be obtained as These can be used as beverages as they are, or can be used by blending with other fruit juices, vegetable juices or mixed juices. Among these, obtaining the composition for suppressing excitement or soothing as a powder is preferable because of excellent storage stability.

[Excitement-suppressing or sedating food or drink or medicine]
The composition for suppressing excitement or sedation containing the tomato-derived substance according to the present invention as an active ingredient can be used as it is as a food or drink, or as a medicine, or as a form containing such a composition, it can also be used as a food or drink or medicine. .

  The food and drink containing the composition for stimulating or suppressing sedation containing the above-mentioned tomato-derived substance as an active ingredient can not only be a food for specified health use, a nutritional functional food, a health food, a functional food or a health supplement. It can also be used as a compounding ingredient for soft drinks and foods. An indication that the composition for suppressing excitement or sedation is used for suppressing excitement or sedation may be attached to the food or drink.

  Although it does not restrict | limit as food / beverage products below, For example, a natural or processed food and drink are mentioned, Preferably it is container drinks, such as fruit juice drink, vegetable juice, fruit vegetable juice, tea drink, and a sports drink. Also preferred are processed foods and processed beverages of tomatoes that use tomatoes as the main component. Examples of the processed food and processed beverage include, but are not limited to, tomato puree, tomato paste, tomato sauce, tomato ketchup, tomato soup, tomato juice, vegetable juice with tomato-derived substance, fruit vegetable juice with tomato-derived substance, tomato Examples include tea drinks with derived substances, sports drinks with tomato-derived substances, tomato cake, and tomato jelly.

  The composition for stimulating or suppressing sedation comprising the above-mentioned tomato-derived substance as an active ingredient can be administered (or ingested) orally in a liquid, powder or solid form, as such or on an appropriate formulation For convenience, it can be mixed with excipients and administered in the form of powder, granules, tablets, capsules and the like. Moreover, a tomato-derived substance can be filled in a capsule as it is, or a powdered or solid tomato-derived substance can be used as a supplement.

  The content of the tomato-derived substance in the food or drink varies depending on the type of the food or drink, but from the viewpoint of obtaining a sufficient excitement suppressing effect or sedative effect without impairing the taste, a solid derived from tomato is obtained with respect to 100 g of the food or drink. It is preferable to contain 0.1-20g in conversion of a thing.

  When the beverage contains the composition for suppressing excitement or sedation containing the above-mentioned tomato-derived substance as an active ingredient, preferably 500 to 100,000 mg, more preferably 1,500 mg in terms of solid matter derived from tomato per 500 mL. 000-100,000 mg. Moreover, it can be set as the formulation mixed suitably with the other additive as a foodstuff or a pharmaceutical.

  The intake (dosage) of the composition for suppressing excitement or sedation containing the above-mentioned tomato-derived substance as an active ingredient can be appropriately adjusted according to the use.

  The number of times of intake (number of times of administration) is not limited to the following, but is preferably 1 to 3 times a day, and the number of times of intake may be increased or decreased as necessary. The number of days of intake (number of days of administration) is not particularly limited, and in the present invention, a food material that is safe even if ingested for a long time, such as tomato, is used, and can be continuously ingested.

  Moreover, the composition for excitation suppression or sedation which uses a tomato origin substance as an active ingredient based on this invention can add a pharmaceutically acceptable excipient | filler as a pharmaceutical, and can use it as a pharmaceutical formulation. Examples of the pharmaceutically acceptable excipient include, but are not limited to, pharmaceutically acceptable proteins such as gelatin and human serum albumin.

  The pharmaceutical preparation is not limited to the following, but for example, oral preparations such as powders, tablets, capsules, granules, fine granules, powders, liquids, syrups, chewable, troches, ointments, gels, creams It can be used as an external preparation such as a patch, an injection, a sublingual, an inhalant, an eye drop or a suppository. Although it does not restrict | limit below as a dosage form of a pharmaceutical formulation, Preferably it is a tablet, a capsule, a powder, a granule, or an injection. The pharmaceutical preparation can also be used as a liquid such as a liquid or a paste.

  The pharmaceutical preparation is useful for suppression of excitement or sedation in animals (particularly mammals), and can be administered to animals (particularly mammals). Examples of mammals include humans, dogs, cats, cows and horses, and humans are preferred.

  The dosage of the above-mentioned pharmaceutical preparation can be appropriately adjusted according to various conditions such as the activity of individual drugs, patient symptoms, age and weight. The dose is preferably 500 to 100,000 mg / time, more preferably 1,000 to 10,000 mg / time, more preferably 3,000 to 500 ml in terms of tomato-derived solid matter. 50,000 mg / time. In addition, since it is the same as the above about the frequency | count of administration and the number of administration days, description is abbreviate | omitted here.

  Moreover, the present inventors discovered that the composition for excitation suppression or sedation which uses a tomato origin substance as an active ingredient has a spontaneous-momentum-lowering effect, as shown in the Example mentioned later. In the present invention, the spontaneous exercise amount lowering effect can be confirmed by reducing the spontaneous exercise amount using a spontaneous exercise amount measurement test. In addition, it is a well-known matter for those skilled in the art that it can be evaluated that it has an excitement suppression action and a sedation action when the spontaneous exercise quantity of a mouse | mouth falls by the spontaneous exercise quantity measurement test using a mouse | mouth. In the present invention, the tomato-derived substance has a spontaneous exercise amount lowering action, so that when it is ingested (administered) to an animal, an excitement suppressing action or a sedative action can be exhibited.

  EXAMPLES Hereinafter, although an Example and a comparative example demonstrate this invention concretely, this invention is not limited to these Examples at all.

[Preparation of tomato serum, synthetic adsorbent adsorbed fraction and synthetic adsorbent non-adsorbed fraction]
As a tomato-derived substance, commercially available concentrated tomato juice Clear Tomato Concentrate 60 ° Brix (manufactured by LYCORED) was used. 5.0 g of Clear Tomato Concentrate 60 ° Brix was diluted with 300 g of distilled water. The Brix of the juice after dilution was 1.1%.

  Further, 50 mL of synthetic adsorbent SP207 (manufactured by Mitsubishi Chemical Corporation) in a wet state in pure water was filled into a glass column, and sufficient pure water was passed through to form a SP207-filled column.

  Subsequently, the obtained dilution was centrifuged under conditions of 3,000 rpm, 25 ° C. and 10 minutes. Then, no. The residue was removed by suction filtration using filter paper No. 2 to obtain a tomato serum.

  The obtained serum was concentrated as it was under reduced pressure and then freeze-dried to obtain a powdery crude extract (hereinafter referred to as “crude extract group”).

  On the other hand, the whole amount of the obtained serum was passed through the glass column (SP207 packed column) at a speed of SV = 6.7 (6 bed volume), and then the tomato serum was sufficiently washed out with 1 bed volume of pure water. Immediately after the start of the passage of tomato serum, the column effluent was sequentially collected at regular intervals and divided as fractions (Fr) 1 to 7 to be non-adsorbed fractions.

  Subsequently, ethanol adjusted to 60% concentration was passed through 3 bed volumes, collection of the column discharge liquid was started, and the ethanol was sequentially collected at regular intervals until the end of the ethanol passage. Fractions (Fr) 8 to 10 were separated and used as adsorption fractions.

  The non-adsorbed fraction (Fr. 1 to Fr. 7) and the adsorbed fraction (Fr. 8 to Fr. 10) were combined and concentrated under reduced pressure, and then freeze-dried to obtain a powdery non-adsorbed fraction. And an adsorbed fraction (hereinafter referred to as “non-adsorbed fraction group”, “fraction I” or “γ-aminobutyric acid fraction”, and “adsorbed fraction group”, “fraction II” or “non-γ- Aminobutyric acid fraction "). Note that “bed” indicates the filling volume (bulk) of the adsorbent.

  The obtained crude extract group, non-adsorbed fraction group, and adsorbed fraction group were each lyophilized and then adjusted to the administration concentration of the animal test described below. Specifically, the crude extract group was 1,000 mg / 5 mL, the non-adsorbed fraction group was 937 mg / 5 mL, and the adsorbed fraction group was 71 mg / 5 mL. In addition, about the administration density | concentration of a non-adsorption fraction group and an adsorption fraction group, it united with the yield with respect to a crude extract group.

Tables 1 and 2 below show the results of various analysis of the animal test samples after adjusting to the respective administration concentrations. Moreover, the graph which shows content of uridine and (gamma) -aminobutyric acid (GABA) of each fraction is shown in FIG. Here, “yield ^* ” in Table 1 represents the yield with respect to 5.0 g of raw material (tomato juice; Brix 60 °). In FIG. 1, Fr. The vertical line at the point No. 7 includes the non-adsorbed fraction (Fr. 1 to Fr. 7) on the left side including the vertical line, and the adsorbed fraction (Fr. 8) on the right side not including the vertical line. To Fr.10).

  This time, the raw material 5.0g / 300mL for SP207 50mL D. W. (Brix 1.1%) was allowed to flow through 6 beds, but the uridine content of the non-adsorbed fraction group was slightly higher, and the recovery rate relative to the crude extract group was 14.6%. This is considered to be because a relatively large amount of uridine was contained in the non-adsorbed part after the 5th bed. On the other hand, regarding γ-aminobutyric acid, the content of the adsorption fraction group was relatively low, and the recovery rate relative to the crude extract group was 5.2%. From this, it was found that γ-aminobutyric acid was hardly adsorbed on SP207.

Example 1 Measurement of Spontaneous Momentum Using a Spontaneous Momentum Measuring Device
For the measurement of the amount of spontaneous movement, mice (CD1 (ICR), male, 7 weeks old; Nippon Charles River Co., Ltd.) were used after preliminary breeding for 1 week. During the experiment, the mice were allowed to freely take water and feed (FR-2, Funabashi Farm Co., Ltd.). The following experiments were performed on the crude extract group, the non-adsorbed fraction group and the adsorbed fraction group obtained as described above, and the group administered with water for injection (control group).

  Using a sonde, the crude extract group, the non-adsorbed fraction group, and the adsorbed fraction group were each orally administered at 5 mL / kg (mouse body weight) taking into account the above-mentioned administration concentration. Forcibly administered orally at 18:00, and after 60 minutes (19:00), the mouse was placed in a cage for measuring spontaneous momentum, and measurement was started using a spontaneous momentum measuring device (NS-AS01, Neuroscience Corporation) ( From 19 o'clock) to 13 hours later (8 o'clock the next day), the spontaneous exercise amount was measured every 30 minutes (the spontaneous exercise amount at 19:00 was not measured). The crude extract group, non-adsorbed fraction group, and adsorbed fraction group were dissolved in 5 mL / kg (mouse body weight) of water for injection (Otsuka Pharmaceutical Factory Co., Ltd.).

  Spontaneous exercise amount was also measured in the same manner as described above for the case where only water for injection was orally administered with a sonde at 5 mL / kg (the body weight of the mouse).

FIG. 2A and FIG. 2B show graphs (n = 10 means ± SE) showing the measurement results of the spontaneous momentum from the start of measurement to 13 hours after the number of groups of n is 10. Moreover, the measurement result of the component content of the tomato test substance is shown in Table 3 and Table 4 below. In FIG. 2, “*” means P <0.05, “**” means P <0.01, and these represent a significant difference with respect to the injection water administration group by Student's t test. In addition, “#” means P <0.05, “##” means P <0.01, and these represent a significant difference with respect to the group administered with water for injection by the Aspin-Welch t test.

  From Tables 3 and 4 and FIG. 2A, in the crude extract group, compared with the control group, the oral period (particularly, the dotted line on the left side of the figure) to the late stage (particularly, the dotted line part on the right side of the figure). ) Over a long period of time). Further, from Tables 3 and 4 and FIG. 2B, it was found that the non-adsorbed fraction group (fraction I, γ-aminobutyric acid fraction) exhibited a significant spontaneous momentum reduction effect as compared to the control group. Such a result can be presumed that the non-adsorbed fraction group exerts an effect on suppression of excitation and sedation due to γ-aminobutyric acid (GABA) present in a large amount in fraction I.

  On the other hand, it was confirmed that the adsorbed fraction group (fraction II, non-γ-aminobutyric acid fraction) also exerted a significant spontaneous momentum reduction effect as compared to the control group. Furthermore, when the case of uridine alone was examined by the same method as in Example 1, no effect was found on the spontaneous momentum (data not shown).

  From the above results, it can be seen that uridine present in a large amount in fraction II (non-γ-aminobutyric acid fraction) does not have an effect on sedation and suppression of excitement at least alone. Specifically, it can be assumed that the combination of uridine and other components present in fraction II, or only the other components present in fraction II, exert an effect on sedation and suppression of excitement. . It has been suggested that as candidates for the other components, there may be aspartic acid, glutamic acid, asparagine, serine, glutamine, arginine and alanine.

  The adsorbed fraction group (fraction II, non-γ-aminobutyric acid fraction) is different from the non-adsorbed fraction group (fraction I, γ-aminobutyric acid fraction), and contains guanosine and adenosine, and also contains cytidine, uridine, and guanosine. And the total content of adenosine is higher than the content of γ-aminobutyric acid, and the content of γ-aminobutyric acid is higher than the total content of aspartic acid, glutamic acid, asparagine, serine, glutamine, arginine and alanine. It was found to have a specific component structure.

  Furthermore, from FIG. 2B, fraction II (dose 71 mg / kg) exerts the same significant spontaneous locomotor activity reduction effect with a much smaller dose compared to fraction I (dose 937 mg / kg). It became clear to do. This indicates that the non-γ-aminobutyric acid fraction (fraction II) has particularly excellent excitement suppressing action and sedation action.

  The tomato-derived substance in the present invention has an excitement suppressing action and a sedative action. The tomato-derived substance in the present invention has industrial applicability as a composition for suppressing excitement or sedation for foods and drinks and pharmaceuticals.

Claims (8)

  A composition for suppressing or suppressing sedation comprising a tomato-derived substance as an active ingredient.   The composition for excitation suppression or sedation according to claim 1, wherein the tomato-derived substance is a non-γ-aminobutyric acid fraction.   The composition for stimulating or suppressing sedation according to claim 1 or 2, wherein the tomato-derived substance contains guanosine or adenosine.   The composition for stimulating or suppressing sedation according to claim 3, wherein the tomato-derived substance further contains cytidine or uridine, and further contains γ-aminobutyric acid in an amount smaller than the total content of cytidine, uridine, guanosine and adenosine. object.   The tomato-derived substance is selected from the group consisting of aspartic acid, glutamic acid, asparagine, serine, glutamine, arginine and alanine, which contains γ-aminobutyric acid and is less in total content than the content of γ-aminobutyric acid. The composition for excitation suppression or sedation of Claim 3 or 4 which further contains 1 or more types.   The composition for stimulating suppression or sedation according to any one of claims 1 to 5, wherein the tomato-derived substance is obtained by adsorbing with a synthetic adsorbent.   The composition for excitation suppression or sedation according to claim 6, wherein the synthetic adsorbent is SP207.   The food-drinks containing the composition for excitation suppression or sedation of any one of Claims 1-7.