JP2011004636A - Method for breeding livestock and poultry by probiotics - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for breeding livestock and poultry by probiotics (utilization of viable bacteria) preventing livestock and poultry from becoming infected with bacillus or developing disease when drinking water, feed and/or the like are/is contaminated with disease-causing germs so as to increase growth effect.SOLUTION: The method for breeding livestock and poultry by probiotics comprises feeding aqueous mixture containing lactobacillus powder obtained by powdering lactobacillus cultured and increased in vegetable culture medium or animal culture medium as drinking water, spray water and/or feed, or feeding solid mixture containing the lactobacillus powder as active ingredient, as feed.

Description

  The present invention relates to a livestock poultry breeding method using probiotics (utilizing live bacteria), and more specifically, livestock such as pigs, cows, horses, sheep and goats, or poultry such as chickens, duck, rabbits and ostriches. If feed, drinking water or the environment is contaminated with pathogenic bacteria, infection or disease can occur, preventing contamination of feed, drinking water or the environment with pathogenic bacteria, and infecting or causing livestock poultry. The present invention relates to a livestock poultry breeding method using probiotics (utilization of live bacteria) that can prevent this from occurring and increase the breeding effect.

  In recent years, side effects of administration of drugs such as antibiotics and disinfectants to livestock and poultry, decreased immunity caused by killing even beneficial bacteria in the body, emergence of resistant bacteria, and when livestock poultry are used as meat In addition, the effects of harmful substances remaining on the meat of livestock poultry on the human body are problematic.

There are many health hazards of livestock poultry due to current livestock poultry management. For example, among multiple diseases in fattening pigs, it is not easy to suffer from swine proliferative enteritis (PPE / Lleis). multisystemic wasting syndrome (PMWS), and there is a fate of 48 kinds of diseases. In particular, improvement of the hygienic environment, which is important for the growth of livestock poultry, has been delayed.
For this reason, breeding is carried out by injecting vaccines or administering antibacterial agents. However, breeding methods due to overcrowding tend to cause stress, and stress is the cause of onset and is susceptible to illness.
Temperature and humidity stress are also important issues. In addition to appropriate temperature control in summer and winter, immunity decreases due to increased stress in the respiratory tract due to decreased humidity, and the onset of pneumonia is a major cause of increased mortality.

  On the other hand, the present inventor makes a fermented feed using Lactobacillus fermentum belonging to lactic acid bacteria and adds the fermented feed, thereby maintaining good health of livestock and poultry without administering antibiotics and other drugs. (See Patent Documents 1 to 3). In addition, the same effect can be obtained with lactic acid-fermenting bacteria belonging to the genus Enterococcus and other Lactobacillus species.

JP 2005-124433 A JP 2005-198536 A JP 2008-295462 A

  The object of the present invention is that livestock such as pigs, cattle, horses, sheep and goats, or poultry such as chickens, duck, moths and ostriches can become infected or diseased when feed, drinking water or the environment is contaminated with pathogenic bacteria. Probiotics that prevent feed, drinking water or the environment from being polluted by pathogens, prevent infection and development of livestock and poultry, and increase the breeding effect It is to provide livestock poultry breeding method (using live bacteria).

  Claim 1 of the present invention for solving the above-mentioned problems is that an aqueous mixture containing lactic acid bacteria powder obtained by pulverizing lactic acid bacteria grown and cultured in a vegetable medium or animal medium as an active ingredient is drinking water and / or spray water and It is a livestock poultry breeding method characterized by being provided and / or raised as feed.

  Claim 2 of the present invention is characterized in that livestock poultry is characterized in that a solid mixture containing, as an active ingredient, lactic acid bacteria powder obtained by pulverizing lactic acid bacteria grown and cultured in a vegetable medium or animal medium is provided as feed. It is a breeding method.

  Claim 3 of the present invention provides an aqueous mixture containing, as an active ingredient, lactic acid bacteria powder obtained by powdering lactic acid bacteria grown and cultured in a plant medium or animal medium as drinking water and / or spray water and / or feed. A livestock poultry breeding method characterized in that a solid mixture containing, as an active ingredient, lactic acid bacteria powder obtained by pulverizing lactic acid bacteria grown and cultured in a vegetable medium or animal medium is provided as feed.

  According to claim 4 of the present invention, in the livestock poultry breeding method according to claim 1 or claim 3, the aqueous mixture is sprayed into the barn to control the humidity according to the seasons, and the temperature of the barn is changed from season to season. It controls according to.

Claim 5 of the present invention is the livestock poultry breeding method according to claim 1, 3 or 4, wherein the concentration of lactic acid bacteria in the aqueous mixture is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces). / Ml).

Claim 6 of the present invention is the livestock poultry breeding method according to claim 2 or claim 3, wherein the concentration of lactic acid bacteria in the solid mixture is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / g). It is characterized by being.

  According to a seventh aspect of the present invention, in the livestock poultry breeding method according to the fifth aspect, an aqueous mixture having a pH of 3.7 to 3.9 is used as the aqueous mixture.

Claim 8 of the present invention is the livestock poultry breeding method according to claim 1, 3 or 4, wherein the concentration of the lactic acid bacteria is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml). When an aqueous mixture is used as drinking water and / or spray water, it is diluted with water so that the concentration of lactic acid bacteria is 1.2 × 10 ² to 1.2 × 10 ³ (cells / ml). To do.

Claim 9 of the present invention is the livestock poultry breeding method according to claim 1, wherein the aqueous mixture having the lactic acid bacteria concentration of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml) is used as a liquid feed. When used, it is characterized in that the concentration of lactic acid bacteria is 1.2 × 10 ⁵ to 1.2 × 10 ⁶ (pieces / ml) by diluting with a general formula feed and water.

Claim 10 of the present invention is the livestock poultry breeding method according to claim 2 or claim 3, wherein the concentration of the lactic acid bacteria is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / g). Is used as a feed, it is diluted with a general blended feed and water so that the concentration of lactic acid bacteria is 1.2 × 10 ⁵ to 1.2 × 10 ⁶ (pieces / g).

The eleventh aspect of the present invention is the livestock poultry breeding method according to the fifth or sixth aspect, wherein the lactic acid bacteria powder is 1 to 10% by mass, the soybean defatted powder is 40 to 60% by mass, and the straw is 10 to 30% by mass. , Using an aqueous mixture containing 5 to 20% by mass of rice bran and further containing water and having a concentration of lactic acid bacteria of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml), Alternatively, a solid mixture having a concentration of lactic acid bacteria of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / g) is used.

  Claim 12 of the present invention is the livestock poultry breeding method according to any one of claims 1 to 11, wherein the lactic acid bacterium belongs to one of the genus Enterococcus and the genus Lactobacillus. It is characterized by being a lactic acid bacterium.

  A thirteenth aspect of the present invention is the livestock poultry breeding method according to any one of the first to twelfth aspects, wherein the lactic acid bacteria powder is produced by a process including the following steps (1) to (4): It is characterized by using lactic acid bacteria powder.

(1) Glucose, yeast extract, and peptone are mixed and heat-sterilized medium is inoculated with an inoculum of lactic acid bacteria and purely cultured in an incubator.
(2) Purified water, various animal or plant nutrients are added to the culture solution obtained in step (1), and lactic acid bacteria are grown and cultured for 72 hours.
(3) The mixture obtained by the growth culture in step (2) is mixed with a mixer to form a slurry.
(4) The slurry obtained in the step (3) is sprayed into a drying chamber and dried by hot air to form a powder, and the obtained powder is heated from the drying chamber by hot air through a delivery tube and is stored at a lower temperature than the drying chamber. To prepare lactic acid bacteria powder.

Claim 1 of the present invention provides an aqueous mixture containing, as an active ingredient, lactic acid bacteria powder obtained by pulverizing lactic acid bacteria grown and cultured in a plant medium or animal medium as drinking water and / or spray water and / or feed. It is a livestock poultry breeding method characterized by breeding,
Domestic animals such as pigs, cows, horses, sheep, goats, and poultry such as chickens, duck, moths, and ostriches can become infected or become sick when drinking water or feed or the environment is contaminated with pathogenic bacteria. By supplying and breeding the aqueous mixture as drinking water and / or spray water and / or feed for oral administration, the drinking water or feed or environment is prevented from being contaminated by pathogenic bacteria, and livestock poultry are infected. It is possible to prevent the onset of disease and increase the nurturing effect.

Claim 2 of the present invention is characterized in that livestock poultry is characterized in that a solid mixture containing, as an active ingredient, lactic acid bacteria powder obtained by pulverizing lactic acid bacteria grown and cultured in a vegetable medium or animal medium is provided as feed. It ’s a breeding method,
Domestic animals such as pigs, cows, horses, sheep, goats, and poultry such as chickens, duck, moths, and ostriches can become infected or become sick when drinking water or feed or the environment is contaminated with pathogenic bacteria. By providing and breeding the solid mixture as a feed, it is possible to prevent the livestock poultry from being infected or sick, and to increase the breeding effect.

Claim 3 of the present invention provides an aqueous mixture containing, as an active ingredient, lactic acid bacteria powder obtained by powdering lactic acid bacteria grown and cultured in a plant medium or animal medium as drinking water and / or spray water and / or feed. , A livestock poultry breeding method characterized in that a solid mixture containing as an active ingredient a lactic acid bacterium powder obtained by pulverizing lactic acid bacteria grown and cultured in a plant medium or an animal medium is provided as a feed and bred.
Domestic animals such as pigs, cows, horses, sheep, goats, and poultry such as chickens, duck, moths, and ostriches can become infected or become sick when drinking water or feed or the environment is contaminated with pathogenic bacteria. Providing the water-based mixture as drinking water and / or spray water and / or feed, and feeding the solid mixture as feed prevents the drinking water, feed, or environment from being contaminated by pathogenic bacteria. Thus, it is possible to prevent the livestock poultry from being infected or sick, and to increase the breeding effect.

According to claim 4 of the present invention, in the livestock poultry breeding method according to claim 1 or claim 3, the aqueous mixture is sprayed into the barn to control the humidity according to the seasons, and the temperature of the barn is changed from season to season. According to the control,
By spraying the water mixture into livestock poultry and controlling the temperature and humidity according to the seasons, and controlling the temperature and humidity of the livestock poultry according to the seasons, drinking water and feed are contaminated by pathogenic bacteria. For example, it is possible to prevent infection and disease of livestock poultry, such as prevention of skin disease by sprayed water adhering to the skin of livestock poultry, There is a further remarkable effect that the breeding effect can be increased.

Claim 5 of the present invention is the livestock poultry breeding method according to claim 1, 3 or 4, wherein the concentration of lactic acid bacteria in the aqueous mixture is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces). / Ml), and
When the concentration of lactic acid bacteria is controlled within the above range, there is a further remarkable effect that the livestock poultry can be more reliably prevented from being infected or diseased.
If the concentration of lactic acid bacteria is less than 1.2 × 10 ⁷ (cells / ml), the effect is impaired, and the long-term storage stability of the aqueous mixture may be impaired, and the concentration of lactic acid bacteria is 1.2 × 10 ⁸ (cells / ml). Exceeding) may impair economic efficiency.

Claim 6 of the present invention is the livestock poultry breeding method according to claim 2 or claim 3, wherein the concentration of lactic acid bacteria in the solid mixture is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / g). It is characterized by being,
When the concentration of lactic acid bacteria is controlled within the above range, there is a further remarkable effect that the livestock poultry can be more reliably prevented from being infected or diseased.
If the concentration of lactic acid bacteria is less than 1.2 × 10 ⁷ (pieces / g), the effect is impaired, and the long-term storage stability of the solid mixture may be impaired, and the concentration of lactic acid bacteria is 1.2 × 10 ⁸ (pieces / piece). If the amount exceeds (ml), economic efficiency may be impaired.

Claim 7 of the present invention is characterized in that in the livestock poultry breeding method according to claim 5, an aqueous mixture having a pH of 3.7 to 3.9 is used as the aqueous mixture.
When the pH is controlled within the above range, the effect is excellent, and when producing drinking water, spray water, liquid feed, or the like, it is possible to further suppress the contamination of the bacteria by pathogenic bacteria.
If the pH is less than 3.7, the economic effect may be impaired. If the pH exceeds 3.9, the effect may be impaired, and the long-term storage stability of the aqueous mixture may be impaired.

Claim 8 of the present invention is the livestock poultry breeding method according to claim 1, 3 or 4, wherein the concentration of the lactic acid bacteria is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml). When an aqueous mixture is used as drinking water and / or spray water, it is diluted with water so that the concentration of lactic acid bacteria is 1.2 × 10 ² to 1.2 × 10 ³ (cells / ml). Is what
When used, it is possible to prevent drinking water from being contaminated by pathogenic bacteria by diluting with water to a concentration of lactic acid bacteria of 1.2 × 10 ² to 1.2 × 10 ³ (cells / ml). It is possible to maintain the preservability of about 1 day and improve usability, and the sprayed water adheres to the skin of livestock poultry, thereby making it possible to prevent skin diseases.
If the concentration of lactic acid bacteria is less than 1.2 × 10 ² (cells / ml), the preservability of about 1 day may not be maintained, and if the concentration of lactic acid bacteria exceeds 1.2 × 10 ³ (cells / ml), economic efficiency is impaired. There is a fear.

Claim 9 of the present invention is the livestock poultry breeding method according to claim 1, wherein the aqueous mixture having the lactic acid bacteria concentration of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml) is used as a liquid feed. When used, it is characterized in that the concentration of lactic acid bacteria is 1.2 × 10 ⁵ to 1.2 × 10 ⁶ (pieces / ml) by diluting with a general formula feed and water,
By adjusting the concentration of lactic acid bacteria to this range, the liquid feed can be prevented from being contaminated by pathogenic bacteria, and the storage stability of about 1 day can be maintained. Can be prevented, so that a further remarkable effect that the breeding effect can be increased is achieved.
If the concentration of lactic acid bacteria is less than 1.2 × 10 ⁵ (cells / ml), the effect may be impaired, and the storage stability of about 1 day may not be maintained, and the concentration of lactic acid bacteria is 1.2 × 10 ⁶ (cells / ml). If the amount exceeds (ml), economic efficiency may be impaired.

Claim 10 of the present invention is the livestock poultry breeding method according to claim 2 or claim 3, wherein the concentration of the lactic acid bacteria is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / g). When using as a feed, it is characterized in that the concentration of lactic acid bacteria is 1.2 × 10 ⁵ to 1.2 × 10 ⁶ (pieces / g) by diluting with a general blended feed and water. Yes,
When used, dilute with general mixed feed and water to make the concentration of lactic acid bacteria 1.2 × 10 ⁵ to 1.2 × 10 ⁶ (pieces / g), so that the feed is contaminated with pathogenic bacteria. It is possible to prevent, maintain storability for about 1 day, improve usability, and prevent livestock and poultry from being infected or diseased. Play.
There is a risk lactobacillus concentration which effect is impaired is less than 1.2 × 10 ⁵ (number / g), also there may not be maintained storage of about one day, the lactic acid bacteria concentration of 1.2 × 10 ⁶ (pieces / If g) is exceeded, the economic efficiency may be impaired.

The eleventh aspect of the present invention is the livestock poultry breeding method according to the fifth or sixth aspect, wherein the lactic acid bacteria powder is 1 to 10% by mass, the soybean defatted powder is 40 to 60% by mass, and the straw is 10 to 30% by mass. Use an aqueous mixture having a concentration of lactic acid bacteria of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml) prepared by blending 5 to 20% by mass of rice bran and further blending moisture, or A solid mixture having a concentration of lactic acid bacteria of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / g) is used,
The aqueous mixture and solid mixture thus prepared can be stored for a long period of time of about two weeks, and an inexpensive aqueous material or solid mixture can be prepared using an inexpensive material, so that the economic effect can be increased. There is a further remarkable effect.

Claim 12 of the present invention is the livestock poultry breeding method according to any one of claims 1 to 11, wherein the lactic acid bacterium belongs to one of the genus Enterococcus and the genus Lactobacillus. It is characterized by being a lactic acid bacterium,
Enterococcus genus and Lactobacillus lactic acid bacteria can be mixed together to increase the density of lactic acid bacteria, complement each other, expand the range of temperature and pH that can be adapted, and increase the utility.

A thirteenth aspect of the present invention is the livestock poultry breeding method according to any one of the first to twelfth aspects, wherein the lactic acid bacteria powder is produced by the steps including the steps (1) to (4). It is characterized by using lactic acid bacteria powder,
There is a further remarkable effect that the lactic acid bacteria powder used in the present invention which is sufficiently dried and can be stored for a long time without impairing lactic acid bacteria (viable bacteria) can be more efficiently and easily prepared.

(A) is a diagram showing the results of growth inhibition experiments of Enterococcus fusium and Lactobacillus fermentum against Salmonella, and (B) is a diagram showing the results of growth inhibition experiments of Enterococcus faecium and Lactobacillus fermentum against E. coli. (C) is a figure which shows the growth-inhibition experiment result of Enterococcus faecium and Lactobacillus fermentum with respect to Clostridium perfringens. It is a figure which shows a part of manufacturing apparatus of the lactic acid bacteria powder used for the livestock poultry breeding method which concerns on embodiment of this invention. It is explanatory drawing which shows the average body weight after the weaning of a piglet until the 101st day. In the growing environment of pigs from weaning (about 25 days after birth) to 150 days of slaughter, the temperature (20 ° C ± 5 ° C) and humidity (65% ± 5%) of the piggery are measured using a humidity temperature control system. The actual measurement chart of a part (8 days from June 7, 2009 to June 14, 2009) of the result of being automatically managed and controlled within the range of is shown. It is explanatory drawing which shows the result of having analyzed the hue of meat, the hardness of fat, food texture, fatty acid, the taste of meat, a sweet component, etc. It is explanatory drawing which shows the result of having analyzed the nutritional component etc. of chicken meat.

  The best mode for carrying out the present invention will be described below with reference to the drawings. In this embodiment, the breeding method when the livestock is a pig will be described, but it can be applied to livestock such as cows, horses, sheep, goats other than pigs or poultry such as chickens, duck, moths and ostriches. Of course. In the present invention, breeding includes everything related to breeding and rearing of livestock poultry.

The details will be described below. In the present invention, lactic acid bacteria grown and cultured in a vegetable medium or animal medium are used.
Hereinafter, the effect of inhibiting the growth of malignant or pathogenic bacteria by plant lactic acid bacteria will be described.
According to experiments conducted by the present inventor, this plant lactic acid bacterium has a growth inhibitory effect on various malignant or pathogenic bacteria (Gram-negative bacteria such as Escherichia coli and Salmonella, Gram-positive bacteria such as Welsh bacteria). I understood.
Lactic acid bacteria suppress the growth of bacteria that become malignant or pathogenic bacteria by lactic acid bacteria themselves or their products (such as lactic acid).
If growth is suppressed, these bacteria will not act as malignant or pathogenic bacteria.
Examples of lactic acid bacteria used in the experiment are Enterococcus faecium and Lactobacillus fermentum of Lactobacillus.

Lactobacillus enterococcus faecium is a cocci form, does not produce gas from glucose, and grows under alkaline conditions of PH 9.6 and acidic conditions of PH 4.8.
High bile resistance. Moreover, it is strong at low temperatures and can survive even at 4 ° C.
Lactobacillus fermentum, a lactic acid bacterium, is gonococcus and produces gas from glucose. Since it has acid resistance, it can survive even under strong acidity (PH 2.0). In addition, it is resistant to heat and can survive at 60 ° C. for 30 minutes.

Next, an experimental method will be described.
First, 1 g of Enterococcus faecium and Lactobacillus fermentum lactic acid bacteria powder (lactic acid bacteria powder will be described in detail later) is dissolved in 10 ml of physiological saline, and a lactic acid sample solution having about 0.9 × 10 ⁹ bacteria / ml is obtained. create.
Next, an MRS agar medium mixed with a sample fungus (E. coli or Salmonella) is placed in a petri dish SH, and the effect of lactic acid bacteria is examined. An experimental filter paper RA cut into a circular shape with a diameter of 4 cm is placed in the center of the petri dish SH to absorb the lactic acid bacteria sample solution. Then, an MRS agar medium in which 1 × 10 ⁷ sample bacteria (E. coli, Salmonella, or Clostridium perfringens) are mixed is poured into the Petri dish SH, left at 37 ° C. for 72 hours, and then observed. The experimental filter paper used was NO26-WA, code 02481180, manufactured by ADVANTEC.

For example, FIG. 1 (b) is a photograph of the results of an experiment in which Escherichia coli, Enterococcus faecium and Lactobacillus fermentum were cultured together on an agar medium. The effects of Enterococcus faecium and Lactobacillus fermentum that inhibit E. coli growth are observed.
A sample filter solution of Enterococcus faecium and Lactobacillus fermentum is infiltrated into the laboratory filter paper RA placed in the center of the petri dish SH. The inhibitory substance produced by Enterococcus faecium and Lactobacillus fermentum from the filter paper penetrates and diffuses in the agar medium and spreads to the periphery. Escherichia coli has already been planted in the entire agar medium and is in an environment where it can grow. Therefore, E. coli is growing in the region RC.
However, in the region RB, E. coli cannot grow and is a transparent region. Region RB is measured by its width WB to determine the inhibitory effect of Enterococcus faecium and Lactobacillus fermentum on E. coli.
Similarly, as a result of the experiment on the inhibitory effect of Enterococcus faecium and Lactobacillus fermentum on Salmonella, it was shown in FIG. 1 (a) that region RB was observed. FIG. 1C shows that the region RB is observed as a result of the experiment on the suppression effect.
As this test method shows, it is important that inhibitors produced by lactic acid bacteria such as Enterococcus faecium and Lactobacillus fermentum inhibit the growth of various malignant or pathogenic bacteria such as Escherichia coli.
In addition, it was confirmed that these lactic acid bacteria cultured in a vegetable medium similarly suppressed the growth of Escherichia coli and Enterococcus faecium, Salmonella and Enterococcus faecium, Salmonella and Lactobacillus fermentum.

Thus, this lactic acid bacterium has a growth inhibitory effect against various malignant or pathogenic bacteria.
In addition, this lactic acid bacterium is economical because it can easily grow in large quantities on a vegetable medium compared to animal lactic acid bacteria grown on a medium mainly containing nutrients collected from animals such as milk and meat. .
The plant lactic acid bacteria may be symbiotic with at least one species, that is, alone (one species) or a combination of two or more species. Combining the genus Enterococcus and Lactobacillus also has the effect of increasing the population density by mixing cocci and bacilli.
Further, in the case of a combination of Enterococcus faecium and Lactobacillus fermentum, they complement each other due to the difference in properties as described above, and the range of temperature and pH that can be adapted as a whole plant lactic acid bacterium is widened to increase the usability.

  Next, a method for producing lactic acid bacteria powder (lactic acid bacteria powder) for raising livestock and poultry as an aqueous mixture of lactic acid bacteria used in the present invention will be described.

  The production process of lactic acid bacteria powder can be divided into (1) pure culture process, (2) growth culture process, (3) slurry process, and (4) powdering process.

(1) In the pure culture process, glucose, yeast extract, and peptone are mixed in purified water, and inoculated lactic acid bacteria (a combination of all inoculated bacteria when combining lactic acid bacteria) to a solution sterilized by heating in an autoclave, Culture in an incubator (for example, 48 hours at 40 ° C.).
In this way, a pure culture solution of mixed lactic acid bacteria is obtained.

(2) The growth culture step is a step of mixing and stirring the purified water, the pure culture bacterial solution, and each nutrient serving as the vegetable culture medium, and performing the growth culture.
Specifically, the above pure culture solution is added to 1.5%, defatted soybean flour 13%, potato starch 6%, (black) molasses 1%, skim milk 4%, natural salt 0.5-1.5%, Lactic acid bacteria are grown by mixing 0.6% sodium glutamate, 0.3% amino acid, and the remainder as purified water in a total amount of 100% and keeping the mixture at 40 ° C. for 48 hours. In addition, a percentage shows the ratio about mass (it is the same also in the following description).

  (3) A slurry process is a process of making the said mixture into a slurry form with a mixer.

(4) The powdering step is a step of spray-drying the slurry mixture with a spray dryer to form powder.
Specifically, as shown in FIG. 2, the manufacturing apparatus 1 is provided with a drying chamber 2, and a nozzle 3 is attached to the upper portion of the drying chamber 2 and a delivery pipe 4 is attached to the lower portion. The delivery pipe 4 extends not only in the lateral direction but also in the upward direction, and the other end is attached to the powder accumulation chamber 5 juxtaposed in the drying chamber 2. The temperature of the inlet EN of the hot air WI in the drying chamber 2 is set to 150 to 180 ° C., the temperature of the outlet EX is set to about 100 ° C., and the turning speed (caused by rotation of a motor (not shown)) is set to about 4000 rpm.

  In the drying chamber 2, the slurry-like mixture MI is sprayed from the nozzle 3, and moisture is quickly removed (dried) by the hot air WI swirling at a high wind speed to become powder. The powder rides on the flow of hot air WI and is sent to the powder accumulation chamber 5 at a lower temperature (40 ° C.) than the drying chamber 2 through the delivery pipe 4.

  Thus, the rapid drying in the drying chamber 2 and the rapid cooling in the powder accumulation chamber 5 can significantly suppress the damage and death of lactic acid bacteria due to high heat. In addition, a protective film made of starch and saccharide is formed on the outer surface during the drying process to protect live bacteria inside, which also suppresses the influence of high heat on lactic acid bacteria.

Thus, by the powdering step, lactic acid bacteria powder having a fine average particle diameter can be obtained by sufficiently drying (to a moisture content of about 3% or less).
This lactic acid bacteria powder had a high viable count of plant lactic acid bacteria, and was 1.2 × 10 ¹⁰ cells / g to 1.2 × 10 ¹¹ cells / g when measured for Enterococcus faecium and Lactobacillus fermentum.
In addition, the lactic acid bacteria powder is sufficiently dry, and the live bacteria inside are protected by a protective film of starch and saccharides, so that it can be stored for a long time (for example, 10 to 20 years in a low humidity ambient environment). Is possible.

The concentration of lactic acid bacteria in the aqueous mixture or solid mixture used in the present invention is not particularly limited, but is preferably 1.2 × 10 ^{7 to} 1.2 × 10 ⁸ (pieces / ml or g). When the concentration of lactic acid bacteria is controlled within the above range, it is excellent in long-term preservation and can more reliably prevent the livestock poultry from being infected or sick.
When the acid bacteria concentration in the aqueous mixture is 1.2 × 10 ⁷ cells / ml, the pH is 3.9, and the lactic acid bacteria concentration in the aqueous mixture is 1.2 × 10 ⁸ cells / ml at the maximum. In that case, the pH is 3.7.
Controlling the pH within the range of 3.7 to 3.9 as an aqueous mixture is excellent in effect, and more reliably ensures that they are contaminated with pathogenic bacteria when preparing drinking water, spray water or liquid feed. Can be suppressed.

In this manner, the lactic acid bacteria can be handled easily because the aqueous mixture or solid mixture used in the present invention can be produced by adding and blending lactic acid bacteria powder that can be stored for a long time each time if necessary.
The aqueous mixture or solid mixture having a lactic acid bacteria concentration of 1.2 × 10 ⁷ (cells / ml or g) to 1.2 × 10 ⁸ (cells / ml or g) used in the present invention becomes a malignant or pathogenic bacterium. Since it has a growth-inhibiting effect on bacteria, it can be used for a long period of storage for about 2 weeks.

As described above, lactic acid bacteria have a growth inhibitory effect against various malignant or pathogenic bacteria. The experimental results of the growth inhibitory effect against Clostridium perfringens which are likely to cause diseases of livestock such as Escherichia coli, Salmonella and pigs, which are easy to reproduce, have been described above with reference to FIGS. 1 (a), (b) and (c).
The growth suppression effect was similarly confirmed about other bacteria. In addition, this plant lactic acid bacterium has a higher acid resistance and a larger number (about 7 to 8 times) reaching the digestive tract than animal lactic acid bacteria grown and cultured in a medium mainly containing nutrients collected from animals.

Next, the management of livestock houses in this livestock poultry breeding method will be described. In this example, management of a piggery will be described.
The livestock poultry breeding method of the present invention that uses lactic acid bacteria and does not use drugs such as antibiotics and disinfectants adjusts the intestinal environment of pigs, relieves stress, and creates a clean environment in the barn.
It is desirable to manage pig houses as follows.
The piggery is arranged in the order of breeding piggery, delivery piggery, weanling piggery, breeding piggery, and shipping piggery, and grow in each piggery according to the pig breeding process.

And it is effective for the control of epidemics as well as the effect of creating a comfortable environment for pigs by always performing clean management of pig manure and pig urine and adjusting the temperature and humidity of the pig house.
For example, with regard to heating in winter, in particular, poor growth of piglets can be prevented and the mortality rate can be reduced.
Humidity control in winter and summer can prevent bronchitis and pneumonia caused by swine virus infection.

Specifically, in the case of piglets, the temperature is 20 to 25 ° C. and the humidity is 55 to 60% in winter, and the temperature is 25 to 30 ° C. and the humidity is 60 to 65% in summer.
In the case of medium pigs, the temperature is 15 to 20 ° C. and the humidity is 60 to 65% in winter, and the temperature is 25 to 30 ° C. and the humidity is 60 to 65% in summer.
In the case of adult pigs, the temperature is adjusted to 10 to 20 ° C. and humidity 65 to 70% in winter, and the temperature is adjusted to 25 to 30 ° C. and humidity 60 to 65% in summer.
In particular, in a breeding piggery, it is effective to install a curtain on the side of the piggery in order to keep the temperature and humidity constant and prevent infectious diseases from the outside.

The humidity of the piggery poultry house is generally adjusted by spraying tap water. However, in the present invention, lactic acid bacteria are added to drinking tap water in the piggery, served as drinking water as an aqueous mixture, and sprayed on the piggery poultry house.
In this way, the drinking water prevents infection and pathogenesis due to pathogenic bacteria like the above-mentioned feed, and while adjusting the humidity, the lactic acid bacteria contained in the sprayed water adhere to the skin of the pig, so that the skin Disease prevention becomes possible.

As described above, it is possible to sell to a user by preparing a lactic acid bacterium powder that is cultured from pure bacteria and can be stored for a long period of time. In addition, an aqueous mixture containing this lactic acid bacterium powder as an active ingredient and a solid If the sex mixture is prepared, it can be stored for about 2 weeks, so it can be sold to the user. The user can dilute it with water to adjust the concentration of lactic acid bacteria and use it on the site of pig farming, poultry farming, etc. It can be applied according to the purpose such as drinking water based on an aqueous mixture, spray water based on an aqueous mixture, solid blended feed based on a solid mixture.
As described above, if the breeding method of the present invention using lactic acid bacteria powder comprehensively for breeding and breeding livestock poultry, no antibiotics or disinfectants are used. On the other hand, it can be kept safely without being affected by the residual harmful substances when it is used as meat or poultry eggs.
In addition, the health condition of livestock poultry is well maintained, growth is good, and delicious pork, chicken and poultry eggs are produced.

The concentration of lactic acid bacteria prepared by blending 1 to 10% by mass of the lactic acid bacteria powder, 40 to 60% by mass of soybean degreased powder, 10 to 30% by mass of rice bran, 5 to 20% by mass of rice bran, and further blending with water. Use an aqueous mixture that is 2 × 10 ^{7 to} 1.2 × 10 ⁸ (cells / ml), or the concentration of lactic acid bacteria is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (cells / g) Use of a solid mixture prevents the feed from being contaminated by pathogenic bacteria, and when the solid mixture is used by oral administration, for example, prevents livestock and poultry from becoming infected and causing disease. The effect can be increased.

An aqueous mixture having a concentration of lactic acid bacteria of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml) can be used as a liquid or paste-like feed. And when used as a liquid feed, the feed is made up of pathogenic bacteria by diluting with general formula feed and water to a concentration of lactic acid bacteria of 1.2 × 10 ⁵ to 1.2 × 10 ⁶ (pieces / ml). Contamination can be prevented, storage stability of about one day can be maintained and convenience of use can be improved, and infection and sickness of livestock poultry can be prevented, so that the breeding effect can be increased.
If the concentration of lactic acid bacteria is less than 1.2 × 10 ⁵ (cells / ml), the preservability of about 1 day may not be maintained, and if the concentration of lactic acid bacteria exceeds 1.2 × 10 ⁶ (cells / ml), economic efficiency is impaired. There is a fear.

  By using an aqueous mixture for feeding, feeding and feeding, it is easy for oral administration to animals and can also be consumed, and by keeping the concentration of lactic acid bacteria within the above range, it can recover from diarrhea and prevent disease. Thus, the effect of raising livestock children can be increased.

  Piglets are generally more likely to develop diarrhea during the lactation period, 15 to 35 days after birth when antibodies transferred from the mother pig decrease due to ingestion of colostrum, and have high mortality even when injected with antibiotics ( For example, about 30%). In the livestock poultry breeding method of the present invention, lactic acid bacteria are orally administered to the offspring of a lactating livestock that has developed diarrhea symptoms by mixing with food, thereby recovering from diarrhea symptoms without using antibiotics. be able to.

The present invention is not limited to the one described in the above-described embodiment, and various design changes can be made within the scope of the matters described in the claims. For example, it is needless to say that detailed numerical values such as manufacturing conditions and environmental conditions described in the embodiments, materials, and the like can be appropriately changed or added.
The claims of this patent mainly demonstrate the effectiveness of the plant culture medium, but it is clear that the same effect can be obtained with lactic acid-fermenting bacteria of the genus Enterococcus and Lactobacillus that are grown and cultured in an animal culture medium.
However, animal culture media are often made from milk and the like, and are not economical. Animal culture media can also be used if it is economically advantageous under lactic acid bacteria culture conditions.

  EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited to these Examples.

Example 1
An aqueous mixture containing, as an active ingredient, lactic acid bacteria powder obtained by powdering lactic acid bacteria grown and grown in a plant medium is provided as drinking water and spray water, and a solid mixture containing the lactic acid bacteria powder as an active ingredient is provided as feed. An example of raising pigs will now be described.

(Preparation of lactic acid bacteria powder)
Glucose, yeast extract, and peptone are mixed with purified water, and an inoculum of lactic acid bacteria (a mixture of Enterococcus faecium and Lactobacillus 1: 1) is added to a solution sterilized by heating in an autoclave and cultured in an incubator (48 at 40 ° C. Time), a pure culture solution of mixed lactic acid bacteria was obtained.
The above pure culture solution is added to 1.5%, defatted soybean flour 13%, potato starch 6%, (black) molasses 1%, skim milk 4%, natural salt 0.5-1.5%, sodium glutamate 0.6 %, Amino acid 0.3%, and the remainder as purified water were mixed in a total amount of 100% and incubated at 40 ° C. for 48 hours to grow lactic acid bacteria, and this mixture was made into a slurry mixture using a mixer.
Next, using the manufacturing apparatus 1 shown in FIG. 2, the slurry mixture MI is rapidly discharged at a temperature of the inlet EN of the hot air WI in the drying chamber 2 of 150 ° C., a temperature of the outlet EX of about 100 ° C., and a rotational speed of about 4000 rpm. The lactic acid bacteria powder (enterococcus) with a fine average particle size of about 3% or less of water content is dried by removing (moisture removed) and powdered, and then sent to the powder storage chamber 5 at about 40 ° C. for rapid cooling. Fesium and Lactobacillus fermentum: 1.2 × 10 ¹¹ / g) were prepared.

(Preparation of solid mixture)
1% lactic acid bacteria powder, 60% soybean defatted powder, 10% rice bran, 20% by mass rice bran, and adjusted the water content to 10% to obtain a solid mixture (concentration of lactic acid bacteria: 1.2 × 10 × 10 ⁹ / g) was prepared.

(Preparation of aqueous mixture)
Tap water was put into a 200 L tank container, 0.02% of the lactic acid bacteria powder was added and mixed to prepare an aqueous mixture (lactic acid bacteria concentration: 1.2 × 10 ⁸ cells / ml).

(Pig breeding)
The solid mixture is further diluted with a general mixed feed and water, mixed with 0.4% of the solid mixture with respect to the total mixed feed, and mixed with the mixed feed (concentration of lactic acid bacteria: 1.2 × 10 ⁶ pieces) / G) as a bait, a daily equivalent of 3% of the pig's body weight was eaten.
The aqueous mixture was mixed 10,000 times with tap water using a Dosatron (trade name, manufactured by SEKINE Co., Ltd., water pressure automatic suction device) from the tank (concentration of lactic acid bacteria: 1.2 × 10 ³ / ml), and tap water Pigs were allowed to drink from the taps (pigs nipples) sucked by the water pressure of the water and provided as drinking water.

In the growing environment of pigs from weaning (about 25 days after birth) to 150 days of slaughter, the temperature (20 ° C ± 5 ° C) and humidity (65% ± 5%) of the piggery are measured using a humidity temperature control system. FIG. 4 shows a part of the result of automatic management and control within the range (8 days from June 7, 2009 to June 14, 2009).
In order to adjust the humidity, the aqueous mixture was mixed 10,000 times with tap water (lactic acid bacteria concentration: 1.2 × 10 ^{2 to} 1.2 × 10 ³ cells / ml) and sprayed on the pig house as spray water.
The sprayed water suppressed the growth of pathogenic bacteria adhering to the skin of livestock poultry and prevented bronchitis and pneumonia caused by swine virus infection.

  The supply of the aqueous mixture as spray water is basically for the purpose of controlling the humidity, and the lactic acid bacteria contained in the sprayed water adhere to the skin of the pig while adjusting the humidity. This makes it possible to prevent skin diseases. When raising pigs, it is an important point from piglets (weaned piglets) to fattening pigs. In particular, the piglet has weak trachea and is often accompanied by bronchitis. It is a very weak animal with respect to climate change. Spraying was always performed by automatic spraying. .

There are 72 pigs subjected to the test.
No drugs such as vaccines or antibiotics were used.
Lactic acid bacteria were used as feed, drinking water, and spray water throughout the period from birth to weaning (25 days after birth) to 150 days of slaughter.
Location: Chiba Prefecture K pig farm.
The mortality rate during the implementation period was zero.
As a result of raising pigs as described above, the results of measuring the average body weight four times during the period from weaning to the 101st day are shown in FIG.

(Comparative Example 1)
Don't use lactic acid bacteria powder, donate tap water as drinking water and spray water, donate general formula feed that does not contain the lactic acid bacteria powder, and use swine vaccines and antibiotics as usual if swine diarrhea symptoms occur As a result of raising pigs in the same manner as in Example 1 except that pigs were raised, the results of measuring the average body weight four times during the period from weaning to 101 days are shown in FIG.
The mortality rate during the implementation period was 10%.

  From FIG. 3, when comparing the average body weights of the pigs of Example 1 and Comparative Example 1, the average body weight of the pigs of Example 1 is larger than the average body weight of the pigs of Comparative Example 1 and clearly shows a significant difference. I understand. This difference between Example 1 and Comparative Example 1 resulted in a clear difference in the feed cost, and it was found that Example 1 is more economical.

(Example 2)
The meat color, fat hardness, texture, fatty acid, umami, sweetness component, etc. of pigs (referred to as Margaret pork) reared with pigs were analyzed in the same manner as in Example 1.
The analysis results are shown in Table 1 and FIG.
Analyst: Genetic ID Inc.

(Comparative Example 2)
In the same manner as in Comparative Example 1, the color of meat, the hardness of fat, the texture, the fatty acid, the taste of meat, the sweetness component, and the like of pigs raised with pigs (referred to as general pork) were analyzed.
The analysis results are shown in Table 1 and FIG.

From Table 1 and FIG. 5, the margaret pork of Example 2 is lower in meat color, the melting point is raised, the fat is hard and non-sticky, and the texture is easy to bite and softer than the pork of Comparative Example 2 , Glutamic acid, meat sweetness element and meat umami element are high and abundant, rich in saturated fatty acid and fragrant, low in linoleic acid and hardly oxidized or deteriorated.
Moreover, the margaret pork's flesh (reddish portion) showed “marbling” and proved high quality. Compared with the quality of normal pork, the results are superior.
It was confirmed that the breeding environment was improved by the livestock poultry breeding method of the present invention, and that it had an excellent effect on the taste of pork.

(Example 3)
An aqueous mixture containing, as an active ingredient, lactic acid bacteria powder obtained by powdering lactic acid bacteria grown and grown in a plant medium is provided as drinking water and spray water, and a solid mixture containing the lactic acid bacteria powder as an active ingredient is provided as feed. An example of raising chickens will now be described.

(Preparation of lactic acid bacteria powder)
Lactic acid bacteria powder (Enterococcus faecium and Lactobacillus fermentum: 1.2 × 10 ¹¹ cells / g) having a fine average particle size with a water content of about 3% or less was prepared by the method described in Example 1.

(Preparation of solid mixture)
According to the method described in Example 1, 1% of the lactic acid bacteria powder, 60% of the soybean defatted powder, 10% of rice bran, 20% by mass of rice bran, and the water content was adjusted to 10% by mass to obtain a solid mixture (concentration of lactic acid bacteria: 1.2 × 10 ⁸ pieces / g) was prepared.

(Preparation of aqueous mixture)
Tap water was put into a 200 L tank container by the method described in Example 1, 0.02% of the lactic acid bacteria powder was added and mixed to obtain an aqueous mixture (lactic acid bacteria concentration: 1.2. × 10 ⁸ cells / ml). Prepared.

(Chicken breeding)
The above-mentioned solid mixture was mixed with a general blended feed with a solid mixture of 0.4% based on the total blended feed, and blended (the concentration of lactic acid bacteria: 1.2 × 10 ⁶ / g) Was eaten every day in an amount equivalent to 3% of the chicken's body weight.
The aqueous mixture was mixed 10,000 times in tap water using Dosatron (trade name, manufactured by SEKINE Co., Ltd., water pressure automatic suction device) from the tank (concentration of lactic acid bacteria: 1.2. × 10 ³ / ml), Chickens were allowed to drink from the taps (chicken nipples) sucked by the water pressure of the taps, provided as drinking water, and sprayed to the poultry house as spray water.

The supply of sprayed water is basically for the purpose of controlling and adjusting the humidity, and while adjusting the humidity, the lactic acid bacteria contained in the sprayed water adhere to the skin of the chicken. Disease prevention becomes possible.
The number of chickens subjected to the test was 8,800 birds in the first test zone, 9,200 birds in the second test zone, and 8,800 birds in the third test zone.

Implementation was performed three times.
First implementation period: January 6, 2009 to February 10, 2009 Second implementation period: March 8, 2009 to April 10, 2009 Third implementation period: 2009 March 19-April 20, 2009

Location: Hyogo prefecture T broiler.
As described above, chickens are bred three times, the number of wings shipped, the number of non-standard wings, the number of non-standard wings (%), weighted shipping weight, breeding rate, feed demand rate, production efficiency, feed bill, chick bill Tables 2 to 4 show the results of lactic acid bacteria cost / chemical cost, gross profit, etc., respectively.

(Comparative Example 3)
Lactic acid bacteria powder was not used, tap water was provided as drinking water and spray water, general formulated feed containing no lactic acid bacteria powder was provided, and the vaccine and antibiotics were combined as usual. Except that the chickens were raised during the following three implementation periods, the chickens were raised in the same manner as in Example 3, and the number of shipments, number of nonstandard shipments, number of nonstandard shipments (%), weighted shipment weight, breeding rate Tables 2 to 4 show the results of measurement of feed request rate, production efficiency, feed cost, chick cost, lactic acid bacteria cost / chemical cost, gross profit, etc., respectively.
First implementation period: January 8, 2009-February 12, 2009 Second implementation period: March 4, 2009-April 8, 2009 Third implementation period: 2009 March 12 to April 17, 2009

In Tables 2-4, when Example 3 is compared with Comparative Example 3, the case of Example 3 using the livestock poultry breeding method of the present invention requires the breeding rate, production efficiency, feed cost, It was found that superior comparison results were obtained for all management indicators such as chick cost and gross profit, and the superiority in the case of Example 3 was revealed all three times.
However, in the third time of Example 3, the use of lactic acid bacteria and a combination of vaccine / antibacterial agent and the like were tried as trials, and therefore, in the case of Example 3, the cost of Example 3 is higher in comparison of the lactic acid bacteria cost / chemical cost. However, in the first and second rounds, the cost of Example 3 is cheaper. As a direct cost comparison, not only was the effectiveness of Example 3 proved, but the broiler productivity and management proved the effectiveness of the livestock poultry breeding method of the present invention.

Example 4
A solid mixture using alpha lact as a lactic acid bacterium is mixed in a general formula feed with 0.4% of the total formula feed, and the mixed formula feed is equivalent to 3% of the body weight of the chicken. The nutritional components of chicken meat (referred to as cherry chicken) were analyzed in the same manner as in Example 3 except that the amount was eaten every day (breding place, Malaysian chicken farm). Ten animals were sampled from among 1000 breeds. Broilers are 42 days old and have an average weight of 2.1 kg.
The analysis results are shown in Table 5 and FIG.
An amount corresponding to 3% of the weight of a chicken, with the solid mixture mixed in a general mixed feed with a solid mixture of 0.4% based on the total mixed feed, Was eaten every day.

(Comparative Example 4)
The nutritional components and the like of chicken meat (referred to as normal chicken) were analyzed in the same manner as in Example 4 except that chickens were raised in the same manner as in Comparative Example 3 (bred location, chicken farm in Malaysia).
The analysis results are shown in Table 5 and FIG.

  From Table 5 and FIG. 6, the cherry chicken of Example 4 clearly shows a decrease in fat, a decrease in saturated fat, and an increase in protein compared to the normal chicken of Comparative Example 4. It can be seen that the reduction of cholesterol is remarkable, and minerals are also reduced in response to concerns about excessive intake of minerals from food, and it has excellent nutritional components. In particular, the cholesterol level decreased and the commercial value increased. These figures for the cherry chicken of Example 4 are improved and highly welcomed by consumers interested in food health.

The present invention provides an aqueous mixture containing, as an active ingredient, lactic acid bacteria powder obtained by pulverizing lactic acid bacteria grown and cultured in a vegetable medium or animal medium as drinking water and / or spray water and / or feed, or the lactic acid bacteria It is a livestock poultry breeding method by probiotics (good viable bacteria), characterized by donating and breeding a solid mixture containing powder as an active ingredient,
Domestic animals such as pigs, cattle, horses, sheep, goats and poultry such as chickens, duck, moths, and ostriches are safe because they can become infected or become sick when drinking water, feed, or the environment is contaminated by pathogenic bacteria. Highly effective lactic acid bacteria prevent drinking water, feed or environment from being contaminated by pathogenic bacteria, preventing livestock poultry from being infected or sick without using antibacterial agents or antibiotics As a result, it is possible to increase the training effect, and the industrial utility value is very large.

DESCRIPTION OF SYMBOLS 1 Manufacturing apparatus 2 Drying chamber 3 Nozzle 4 Delivery pipe 5 Powder accumulation chamber MI Mixture WI Hot air EN Inlet EX Outlet SH Petroleum RA Water absorption filter paper RC Sample bacteria area RB Area where sample bacteria are extremely small WB Width

Claims (13)

  A domestic animal characterized in that an aqueous mixture containing, as an active ingredient, a lactic acid bacterium powder obtained by pulverizing lactic acid bacteria grown and cultured in a vegetable medium or an animal medium is provided as drinking water and / or spray water and / or feed. Poultry raising method.   A livestock poultry breeding method characterized in that a solid mixture containing, as an active ingredient, a lactic acid bacteria powder obtained by powdering lactic acid bacteria grown and cultured in a plant medium or an animal medium is provided as feed.   An aqueous mixture containing, as an active ingredient, lactic acid bacteria powder obtained by pulverizing lactic acid bacteria grown and cultured in a vegetable medium or animal medium is provided as drinking water and / or spray water and / or feed, as well as a vegetable medium or animal medium. A livestock poultry breeding method characterized in that a solid mixture containing, as an active ingredient, lactic acid bacteria powder obtained by pulverizing lactic acid bacteria grown and cultured in (1) is provided as feed.   The livestock poultry breeding method according to claim 1 or 3, wherein the aqueous mixture is sprayed into a barn to control humidity according to the seasons, and the temperature of the barn is controlled according to the seasons. . The livestock poultry breeding method according to claim 1, wherein the concentration of lactic acid bacteria in the aqueous mixture is 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (cells / ml). . The livestock poultry breeding method according to claim 2 or 3, wherein the concentration of lactic acid bacteria in the solid mixture is 1.2 x 10 ⁷ to 1.2 x 10 ⁸ (pieces / g).   6. The livestock poultry breeding method according to claim 5, wherein an aqueous mixture having a pH of 3.7 to 3.9 is used as the aqueous mixture. When the aqueous mixture having a concentration of lactic acid bacteria of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml) is used as drinking water and / or spray water, the concentration of lactic acid bacteria is reduced to 1 by diluting with water. The livestock poultry breeding method according to claim 1, 3 or 4, wherein the method is from 2 × 10 ^{2 to} 1.2 × 10 ³ (pieces / ml). When an aqueous mixture having a concentration of lactic acid bacteria of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / ml) is used as a liquid feed, the concentration of lactic acid bacteria is set to 1 by diluting with a general mixed feed and water. 2. The method for raising livestock poultry according to claim 1, wherein the method is 2 × 10 ^{5 to} 1.2 × 10 ⁶ (pieces / ml). When the solid mixture having a concentration of lactic acid bacteria of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (pieces / g) is used as a feed, the concentration of lactic acid bacteria is set to 1. The livestock poultry breeding method according to claim 2 or 3, wherein the method is 2 × 10 ^{5 to} 1.2 × 10 ⁶ (pieces / g). The concentration of lactic acid bacteria is 1-10% by mass of the lactic acid bacteria powder, 40-60% by mass of soybean defatted powder, 10-30% by mass of rice bran, 5-20% by mass of rice bran, and further mixed with water. Use an aqueous mixture that is 1.2 × 10 ^{7 to} 1.2 × 10 ⁸ (cells / ml), or a lactic acid bacteria concentration of 1.2 × 10 ⁷ to 1.2 × 10 ⁸ (cells / g). The livestock poultry breeding method according to claim 5 or 6, wherein a solid mixture is used.   The livestock poultry breeding method according to any one of claims 1 to 11, wherein the lactic acid bacterium is one or more lactic acid bacteria belonging to any of the genus Enterococcus and Lactobacillus. The livestock poultry breeding method according to any one of claims 1 to 12, wherein the lactic acid bacteria powder is a lactic acid bacteria powder produced by a process including the following steps (1) to (4). .
(1) Glucose, yeast extract, and peptone are mixed and heat-sterilized medium is inoculated with an inoculum of lactic acid bacteria and purely cultured in an incubator.
(2) Purified water, various animal or plant nutrients are added to the culture solution obtained in step (1), and lactic acid bacteria are grown and cultured for 72 hours.
(3) The mixture obtained by the growth culture in step (2) is mixed with a mixer to form a slurry.
(4) The slurry obtained in the step (3) is sprayed into a drying chamber and dried by hot air to form a powder, and the obtained powder is heated from the drying chamber by hot air through a delivery tube and is stored at a lower temperature than the drying chamber. To prepare lactic acid bacteria powder.

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