JP2010519935A5 - - Google Patents
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- JP2010519935A5 JP2010519935A5 JP2009552855A JP2009552855A JP2010519935A5 JP 2010519935 A5 JP2010519935 A5 JP 2010519935A5 JP 2009552855 A JP2009552855 A JP 2009552855A JP 2009552855 A JP2009552855 A JP 2009552855A JP 2010519935 A5 JP2010519935 A5 JP 2010519935A5
- Authority
- JP
- Japan
- Prior art keywords
- droplet
- hydrogen peroxide
- assay
- mediated
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- MHAJPDPJQMAIIY-UHFFFAOYSA-N hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims 14
- 238000004166 bioassay Methods 0.000 claims 9
- 239000003153 chemical reaction reagent Substances 0.000 claims 5
- 230000001404 mediated Effects 0.000 claims 5
- 239000012491 analyte Substances 0.000 claims 4
- 239000012530 fluid Substances 0.000 claims 4
- 108020005203 Oxidases Proteins 0.000 claims 3
- 229940072417 Peroxidase Drugs 0.000 claims 3
- 102000003992 Peroxidases Human genes 0.000 claims 3
- 108090000437 Peroxidases Proteins 0.000 claims 3
- 239000000945 filler Substances 0.000 claims 3
- 210000004369 Blood Anatomy 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 108010015776 EC 1.1.3.4 Proteins 0.000 claims 1
- 229940116332 GLUCOSE OXIDASE Drugs 0.000 claims 1
- 239000004366 Glucose oxidase Substances 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 230000001809 detectable Effects 0.000 claims 1
- 230000005684 electric field Effects 0.000 claims 1
- 238000001962 electrophoresis Methods 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 235000019420 glucose oxidase Nutrition 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- 229920002545 silicone oil Polymers 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
Claims (17)
- 被分析物を検出するために過酸化水素アッセイを実施する方法であって、電極が媒介する小滴操作を使用して過酸化水素アッセイ試薬および試料を、フィラー流体によって取り囲まれておりかつ１ｎＬ〜１０μＬの範囲の体積を有する小滴の中へと混合する工程と、前記小滴の中で前記アッセイを実施して、検出可能な信号を得る工程とを含む方法。
- 前記過酸化水素アッセイ試薬が、オキシダーゼと、ペルオキシダーゼと、ペルオキシダーゼおよび過酸化水素の存在下で信号分子を生成する基質とを含む、請求項１に記載の方法。
- 前記被分析物がグルコースを含み、前記オキシダーゼがグルコースオキシダーゼを含む、請求項２に記載の方法。
- 前記試料が、生体液を含み、前記ペルオキシダーゼが、線形の結果を生成する量で提供される、請求項２に記載の方法。
- 前記結果が、５秒未満である遅延時間を有する、請求項４に記載の方法。
- 前記小滴が１０ｎＬ〜１μＬの範囲のサイズを有する、請求項１に記載の方法。
- 前記小滴が１ｎＬ〜５００ｎＬの範囲のサイズを有する、請求項１に記載の方法。
- 前記過酸化水素アッセイ試薬および試料を混合する工程が、１以上の小滴操作を用いて１以上の試料小滴を１以上の過酸化水素アッセイ試薬と混合する工程を含む、請求項１に記載の方法。
- 前記小滴操作の１以上が、電極が媒介する小滴操作を含む、請求項８に記載の方法。
- 前記小滴操作の１以上が、電場が媒介する小滴操作を含む、請求項８に記載の方法。
- 前記小滴操作の１以上が、液滴駆動が媒介する小滴操作を含む、請求項８に記載の方法。
- 前記小滴操作の１以上が、電気泳動が媒介する小滴操作を含む、請求項８に記載の方法。
- 前記被分析物が、オキシダーゼの存在下で過酸化水素を生成することができる血液中に存在する代謝産物からなる群から選択される、請求項１に記載の方法。
- 前記小滴が、前記小滴と非混和性であるフィラー流体によって取り囲まれている、請求項１に記載の方法。
- 前記フィラー流体がシリコンオイルを含む、請求項１４に記載の方法。
- 前記アッセイが、前記被分析物のすべてが消費されるフラッシュアッセイを含む、請求項１に記載の方法。
- 前記フラッシュアッセイを実施するための試料および試薬が、１以上の小滴操作を用いて、小滴アクチュエータ上で、センサの存在下で混合される、請求項１６に記載の方法。
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US89297107P | 2007-03-05 | 2007-03-05 | |
| US60/892,971 | 2007-03-05 | ||
| US98018607P | 2007-10-16 | 2007-10-16 | |
| US60/980,186 | 2007-10-16 | ||
| PCT/US2008/055891 WO2008109664A1 (en) | 2007-03-05 | 2008-03-05 | Hydrogen peroxide droplet-based assays |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010519935A JP2010519935A (ja) | 2010-06-10 |
| JP2010519935A5 true JP2010519935A5 (ja) | 2011-04-21 |
| JP5491203B2 JP5491203B2 (ja) | 2014-05-14 |
Family
ID=39738768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009552855A Expired - Fee Related JP5491203B2 (ja) | 2007-03-05 | 2008-03-05 | 過酸化水素小滴ベースのアッセイ |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8426213B2 (ja) |
| EP (1) | EP2118303B1 (ja) |
| JP (1) | JP5491203B2 (ja) |
| KR (1) | KR101523754B1 (ja) |
| CN (1) | CN101663403B (ja) |
| AU (1) | AU2008222860B2 (ja) |
| BR (1) | BRPI0807681A2 (ja) |
| CA (1) | CA2716603A1 (ja) |
| WO (1) | WO2008109664A1 (ja) |
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| WO2011137533A1 (en) | 2010-05-05 | 2011-11-10 | The Governing Council Of The University Of Toronto | Method of processing dried samples using digital microfluidic device |
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| EP2707131B1 (en) | 2011-05-09 | 2019-04-24 | Advanced Liquid Logic, Inc. | Microfluidic feedback using impedance detection |
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| EP2729792A4 (en) | 2011-07-06 | 2015-03-18 | Advanced Liquid Logic Inc | STORING REAGENTS ON A DROPLET HANDLING ACTUATOR |
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-
2008
- 2008-03-05 US US12/529,052 patent/US8426213B2/en not_active Expired - Fee Related
- 2008-03-05 WO PCT/US2008/055891 patent/WO2008109664A1/en active Application Filing
- 2008-03-05 EP EP08743682.0A patent/EP2118303B1/en not_active Not-in-force
- 2008-03-05 AU AU2008222860A patent/AU2008222860B2/en not_active Ceased
- 2008-03-05 JP JP2009552855A patent/JP5491203B2/ja not_active Expired - Fee Related
- 2008-03-05 BR BRPI0807681A patent/BRPI0807681A2/pt not_active IP Right Cessation
- 2008-03-05 CA CA2716603A patent/CA2716603A1/en not_active Abandoned
- 2008-03-05 KR KR1020097020354A patent/KR101523754B1/ko not_active IP Right Cessation
- 2008-03-05 CN CN2008800072055A patent/CN101663403B/zh not_active Expired - Fee Related
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