JP2010502962A - 電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置 - Google Patents
電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置 Download PDFInfo
- Publication number
- JP2010502962A JP2010502962A JP2009526932A JP2009526932A JP2010502962A JP 2010502962 A JP2010502962 A JP 2010502962A JP 2009526932 A JP2009526932 A JP 2009526932A JP 2009526932 A JP2009526932 A JP 2009526932A JP 2010502962 A JP2010502962 A JP 2010502962A
- Authority
- JP
- Japan
- Prior art keywords
- well
- collection
- electrophoresis
- loading
- cassette
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 373
- 238000000034 method Methods 0.000 title claims abstract description 270
- 239000000499 gel Substances 0.000 title abstract description 259
- 230000005684 electric field Effects 0.000 claims abstract description 128
- 239000000872 buffer Substances 0.000 claims abstract description 57
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims description 97
- 238000000926 separation method Methods 0.000 claims description 83
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 36
- 238000010367 cloning Methods 0.000 claims description 35
- 102000004169 proteins and genes Human genes 0.000 claims description 31
- 238000012544 monitoring process Methods 0.000 claims description 30
- 108090000623 proteins and genes Proteins 0.000 claims description 30
- 229920000936 Agarose Polymers 0.000 claims description 22
- 238000011282 treatment Methods 0.000 claims description 20
- 239000012634 fragment Substances 0.000 claims description 18
- 229920002401 polyacrylamide Polymers 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 9
- 239000012146 running buffer Substances 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 6
- 238000004949 mass spectrometry Methods 0.000 claims description 5
- 230000000712 assembly Effects 0.000 abstract description 17
- 238000000429 assembly Methods 0.000 abstract description 17
- 239000000523 sample Substances 0.000 description 146
- 238000012800 visualization Methods 0.000 description 35
- 239000011159 matrix material Substances 0.000 description 30
- 150000007523 nucleic acids Chemical class 0.000 description 26
- 108020004414 DNA Proteins 0.000 description 24
- 239000000975 dye Substances 0.000 description 23
- 108020004707 nucleic acids Proteins 0.000 description 23
- 102000039446 nucleic acids Human genes 0.000 description 23
- 238000002955 isolation Methods 0.000 description 22
- 102000004196 processed proteins & peptides Human genes 0.000 description 22
- 150000001413 amino acids Chemical class 0.000 description 21
- 239000011324 bead Substances 0.000 description 20
- 239000000126 substance Substances 0.000 description 18
- 239000002245 particle Substances 0.000 description 16
- 108091034117 Oligonucleotide Proteins 0.000 description 15
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
- 239000000203 mixture Substances 0.000 description 13
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 12
- 230000000670 limiting effect Effects 0.000 description 12
- -1 Cassettes Substances 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 10
- 229920001184 polypeptide Polymers 0.000 description 10
- 230000003851 biochemical process Effects 0.000 description 9
- 230000002441 reversible effect Effects 0.000 description 9
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 8
- 238000013508 migration Methods 0.000 description 8
- 230000005012 migration Effects 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 230000035620 dolor Effects 0.000 description 7
- 229920000642 polymer Polymers 0.000 description 7
- 102000053602 DNA Human genes 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000009423 ventilation Methods 0.000 description 6
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 108010024636 Glutathione Proteins 0.000 description 5
- 230000031018 biological processes and functions Effects 0.000 description 5
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 5
- 229960005542 ethidium bromide Drugs 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 4
- 108010038807 Oligopeptides Proteins 0.000 description 4
- 102000015636 Oligopeptides Human genes 0.000 description 4
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 230000003196 chaotropic effect Effects 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- 238000005342 ion exchange Methods 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 239000012474 protein marker Substances 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000000378 two-dimensional separation method Methods 0.000 description 4
- 238000005349 anion exchange Methods 0.000 description 3
- 238000003491 array Methods 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000005341 cation exchange Methods 0.000 description 3
- 230000002860 competitive effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 229960003180 glutathione Drugs 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 3
- 238000007794 visualization technique Methods 0.000 description 3
- QZTKDVCDBIDYMD-UHFFFAOYSA-N 2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid Chemical compound NC(=O)CN(CC(O)=O)CC(O)=O QZTKDVCDBIDYMD-UHFFFAOYSA-N 0.000 description 2
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- ACERFIHBIWMFOR-UHFFFAOYSA-N 2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)azaniumyl]propane-1-sulfonate Chemical compound OCC(C)(C)NCC(O)CS(O)(=O)=O ACERFIHBIWMFOR-UHFFFAOYSA-N 0.000 description 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 2
- RZQXOGQSPBYUKH-UHFFFAOYSA-N 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCC(CO)(CO)NCC(O)CS(O)(=O)=O RZQXOGQSPBYUKH-UHFFFAOYSA-N 0.000 description 2
- XCBLFURAFHFFJF-UHFFFAOYSA-N 3-[bis(2-hydroxyethyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCCN(CCO)CC(O)CS(O)(=O)=O XCBLFURAFHFFJF-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 108010074860 Factor Xa Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 description 2
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 229920002319 Poly(methyl acrylate) Polymers 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229910002804 graphite Inorganic materials 0.000 description 2
- 239000010439 graphite Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000011133 lead Substances 0.000 description 2
- 239000004973 liquid crystal related substance Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- 229920005615 natural polymer Polymers 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052763 palladium Inorganic materials 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 229920001059 synthetic polymer Polymers 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 description 1
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 1
- AJTVSSFTXWNIRG-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid Chemical compound OCC[NH+](CCO)CCS([O-])(=O)=O AJTVSSFTXWNIRG-UHFFFAOYSA-N 0.000 description 1
- 125000000954 2-hydroxyethyl group Chemical group [H]C([*])([H])C([H])([H])O[H] 0.000 description 1
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 description 1
- 239000007991 ACES buffer Substances 0.000 description 1
- 108700016232 Arg(2)-Sar(4)- dermorphin (1-4) Proteins 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
- 150000008574 D-amino acids Chemical class 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 description 1
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical compound OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 description 1
- GIZQLVPDAOBAFN-UHFFFAOYSA-N HEPPSO Chemical compound OCCN1CCN(CC(O)CS(O)(=O)=O)CC1 GIZQLVPDAOBAFN-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 150000008575 L-amino acids Chemical class 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- 239000007997 Tricine buffer Substances 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 208000034953 Twin anemia-polycythemia sequence Diseases 0.000 description 1
- 108010006886 Vitrogen Proteins 0.000 description 1
- 150000001241 acetals Chemical class 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
- 239000007998 bicine buffer Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 108010053098 biotin receptor Proteins 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 210000001520 comb Anatomy 0.000 description 1
- 238000000748 compression moulding Methods 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 239000000112 cooling gas Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
- OGGXGZAMXPVRFZ-UHFFFAOYSA-M dimethylarsinate Chemical compound C[As](C)([O-])=O OGGXGZAMXPVRFZ-UHFFFAOYSA-M 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 238000004049 embossing Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- TWYVVGMYFLAQMU-UHFFFAOYSA-N gelgreen Chemical compound [I-].[I-].C1=C(N(C)C)C=C2[N+](CCCCCC(=O)NCCCOCCOCCOCCCNC(=O)CCCCC[N+]3=C4C=C(C=CC4=CC4=CC=C(C=C43)N(C)C)N(C)C)=C(C=C(C=C3)N(C)C)C3=CC2=C1 TWYVVGMYFLAQMU-UHFFFAOYSA-N 0.000 description 1
- JGBUYEVOKHLFID-UHFFFAOYSA-N gelred Chemical compound [I-].[I-].C=1C(N)=CC=C(C2=CC=C(N)C=C2[N+]=2CCCCCC(=O)NCCCOCCOCCOCCCNC(=O)CCCCC[N+]=3C4=CC(N)=CC=C4C4=CC=C(N)C=C4C=3C=3C=CC=CC=3)C=1C=2C1=CC=CC=C1 JGBUYEVOKHLFID-UHFFFAOYSA-N 0.000 description 1
- 239000011544 gradient gel Substances 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012806 monitoring device Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 239000012811 non-conductive material Substances 0.000 description 1
- 229910052755 nonmetal Inorganic materials 0.000 description 1
- 238000003499 nucleic acid array Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- SCUZVMOVTVSBLE-UHFFFAOYSA-N prop-2-enenitrile;styrene Chemical compound C=CC#N.C=CC1=CC=CC=C1 SCUZVMOVTVSBLE-UHFFFAOYSA-N 0.000 description 1
- 238000003498 protein array Methods 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002342 ribonucleoside Substances 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000010979 ruby Substances 0.000 description 1
- 229910001750 ruby Inorganic materials 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 1
- 229910001488 sodium perchlorate Inorganic materials 0.000 description 1
- UYCAUPASBSROMS-AWQJXPNKSA-M sodium;2,2,2-trifluoroacetate Chemical compound [Na+].[O-][13C](=O)[13C](F)(F)F UYCAUPASBSROMS-AWQJXPNKSA-M 0.000 description 1
- YCLWMUYXEGEIGD-UHFFFAOYSA-M sodium;2-hydroxy-3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonate Chemical compound [Na+].OCCN1CCN(CC(O)CS([O-])(=O)=O)CC1 YCLWMUYXEGEIGD-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 229920000638 styrene acrylonitrile Polymers 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003856 thermoforming Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrostatic Separation (AREA)
- Peptides Or Proteins (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82421006P | 2006-08-31 | 2006-08-31 | |
| US82951706P | 2006-10-13 | 2006-10-13 | |
| PCT/US2007/077383 WO2008028127A1 (en) | 2006-08-31 | 2007-08-31 | Methods, cassettes, gels and apparatuses for isolation and collection of biomolecules from electrophoresis gels |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010502962A true JP2010502962A (ja) | 2010-01-28 |
| JP2010502962A5 JP2010502962A5 (enExample) | 2011-05-26 |
Family
ID=39136297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009526932A Pending JP2010502962A (ja) | 2006-08-31 | 2007-08-31 | 電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US20080057557A1 (enExample) |
| EP (1) | EP2062036A4 (enExample) |
| JP (1) | JP2010502962A (enExample) |
| WO (1) | WO2008028127A1 (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014517314A (ja) * | 2011-06-16 | 2014-07-17 | ブリティッシュ コロンビア キャンサー エイジェンシー ブランチ | 核酸の自動サイズ選択 |
| WO2020012805A1 (ja) * | 2018-07-12 | 2020-01-16 | 株式会社日立ハイテクノロジーズ | 電気泳動方法、電気泳動システム及び電気泳動ゲル |
| JPWO2023002775A1 (enExample) * | 2021-07-19 | 2023-01-26 | ||
| US12467897B2 (en) | 2020-09-02 | 2025-11-11 | Hitachi High-Tech Corporation | Electrophoresis device and biological substance recovery method |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200940983A (en) * | 2007-10-31 | 2009-10-01 | Arkray Inc | Control liquid identifying method and analysis device |
| US8361299B2 (en) * | 2008-10-08 | 2013-01-29 | Sage Science, Inc. | Multichannel preparative electrophoresis system |
| CA2738049C (en) * | 2008-10-08 | 2017-06-13 | Sage Science, Inc. | Multichannel preparative electrophoresis system |
| TWI358539B (en) * | 2008-12-09 | 2012-02-21 | Univ Nat Taiwan | Integrated electrophoresis device and operation th |
| JP5304352B2 (ja) * | 2009-03-13 | 2013-10-02 | 凸版印刷株式会社 | ろ紙を不要とした転写装置 |
| US20100326827A1 (en) * | 2009-06-24 | 2010-12-30 | Shang-Chi Lin | Method and apparatus for isolating and purifying bio-molecules |
| EP2470891B1 (en) * | 2009-08-24 | 2014-12-10 | Life Technologies Corporation | Gel electrophoresis, imaging, and analysis methods, devices, systems, and materials |
| JP2012029676A (ja) * | 2010-07-07 | 2012-02-16 | Sony Corp | 核酸濃縮回収用カートリッジ、核酸濃縮回収方法、及び該カートリッジの製造方法 |
| CA2887341C (en) | 2012-10-12 | 2021-03-16 | Sage Science, Inc. | Side-eluting molecular fractionator |
| JP6317771B2 (ja) * | 2013-03-07 | 2018-04-25 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 電気泳動分離装置およびそれを使用するための方法 |
| CN103555799B (zh) * | 2013-10-26 | 2015-09-23 | 吉林大学 | 一种免疫活性肽冻干粉及其制备方法 |
| WO2016061416A1 (en) * | 2014-10-15 | 2016-04-21 | Sage Science, Inc. | Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation |
| CN108139354A (zh) | 2015-09-22 | 2018-06-08 | 生物辐射实验室股份有限公司 | 电泳容器和方法 |
| JP7054678B2 (ja) | 2015-11-20 | 2022-04-14 | ワシントン・ユニバーシティ | ゲノムｄｎａ断片の標的化された精製のための調製用電気泳動方法 |
| US11867661B2 (en) | 2017-04-07 | 2024-01-09 | Sage Science, Inc. | Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification |
| CN109470760B (zh) * | 2018-12-26 | 2024-05-31 | 天康生物制药有限公司 | 用于制备琼脂糖凝胶的装置、琼脂糖凝胶电泳方法和应用 |
| JP7373923B2 (ja) * | 2019-06-20 | 2023-11-06 | 株式会社日立ハイテク | 生体物質回収方法 |
| WO2021096092A1 (ko) * | 2019-11-12 | 2021-05-20 | (주) 티맥 | 수평형 전기영동장치 |
| DE102020109087A1 (de) * | 2020-04-01 | 2021-10-07 | MobiCron GmbH | Elektrophoresevorrichtung zur Verwendung bei einem Electroclearing-Verfahren |
| EP3978914A1 (en) * | 2020-09-30 | 2022-04-06 | iCare Diagnostics International Co. Ltd. | Electrode, ph measuring device, and electrophoresis detection system |
| CN113607714B (zh) * | 2021-10-08 | 2022-01-11 | 成都齐碳科技有限公司 | 分子膜成膜或表征器件、装置、方法以及生物芯片 |
| EP4419901A4 (en) * | 2021-10-21 | 2025-11-19 | Zadeh Cameron O | HIGH-SPEED MULTIPLEXED ELECTROPHORETIC GEL APPARATUS AND ASSOCIATED PROCESSES |
| WO2025233688A1 (en) | 2024-05-08 | 2025-11-13 | Axor Biosystems Inc. | Microplate-based electrophoresis system |
| CN118547047A (zh) * | 2024-05-29 | 2024-08-27 | 苏州中析生物信息有限公司 | 一种核酸分离纯化方法及分离纯化装置 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63222254A (ja) * | 1987-03-12 | 1988-09-16 | Kusano Hiroshi | 電気泳動装置用回収セル |
| JP2004155687A (ja) * | 2002-11-05 | 2004-06-03 | Forestry & Forest Products Research Institute | 等電点電気泳動法によるタンパク質溶出方法。 |
| JP2004290109A (ja) * | 2003-03-27 | 2004-10-21 | Matsushita Electric Ind Co Ltd | 生体物質回収装置 |
| JP2004294317A (ja) * | 2003-03-27 | 2004-10-21 | Matsushita Electric Ind Co Ltd | 生体物質回収装置 |
| JP2004309326A (ja) * | 2003-04-08 | 2004-11-04 | Yokogawa Electric Corp | 生体高分子の抽出方法および抽出装置 |
| JP2005241477A (ja) * | 2004-02-26 | 2005-09-08 | Japan Science & Technology Agency | 電気泳動用ゲルおよびその利用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1530326A (en) * | 1923-01-04 | 1925-03-17 | Harry A Prindle | Centrifugal pump |
| US2827268A (en) * | 1953-12-07 | 1958-03-18 | Staaf Gustaf Adolf | Liquid transporting apparatus |
| US3512375A (en) * | 1968-11-27 | 1970-05-19 | Sealectro Corp | Flexible coupling for shafts |
| US3856272A (en) * | 1972-06-08 | 1974-12-24 | Richards Of Rockford Inc | Floating mixer |
| US4030859A (en) * | 1975-09-02 | 1977-06-21 | Lake Aid Inc. | Floating aerator having means to vary the length of the draft pipe |
| US4179243A (en) * | 1977-06-21 | 1979-12-18 | Aide Richard J | Floatation pump device |
| US4151065A (en) * | 1978-01-30 | 1979-04-24 | The Regents Of The University Of California | Horizontal slab gel electrophoresis |
| US4764313A (en) * | 1986-11-03 | 1988-08-16 | Sunset Solar Systems Ltd. | Air driven water circulation mill |
| US5139637A (en) * | 1991-03-13 | 1992-08-18 | Macconnell William P | Plasmid purification system and method |
| US5217593A (en) * | 1991-03-13 | 1993-06-08 | Macconnell William P | Nucleic acid purification system and method |
| US5384022A (en) * | 1993-09-08 | 1995-01-24 | Cornell Research Foundation, Inc. | Method and apparatus for electrophoretic DNA band isolation |
| US6379516B1 (en) * | 1995-04-26 | 2002-04-30 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| US5785835A (en) * | 1996-04-12 | 1998-07-28 | One Lambda | Electrophoresis method and devices |
| US5800691A (en) * | 1996-12-23 | 1998-09-01 | Guest Elchrom Scientific Ag | Electrophoresis gels containing sample wells with enlarged loading area |
| US6146511A (en) * | 1998-01-30 | 2000-11-14 | The Perkin-Elmer Corporation | Electrophoretic nucleic acid purification method |
| US6521111B1 (en) * | 2000-04-10 | 2003-02-18 | Invitrogen Corporation | Methods and articles for labeling polymer gels |
| US6562213B1 (en) * | 2000-08-30 | 2003-05-13 | Ethrog Biotechnology Ltd. | Electrophoresis apparatus for simultaneous loading of multiple samples |
| US6602391B2 (en) * | 2001-03-05 | 2003-08-05 | Vladimir B. Serikov | Apparatus and method for combined capillary separation and blotting of biological macromolecules |
| US6632340B2 (en) * | 2001-03-23 | 2003-10-14 | Bio-Rad Laboratories, Inc. | Precast gel and tray combination for submerged gel electrophoresis |
| US7727716B2 (en) * | 2003-09-30 | 2010-06-01 | Life Technologies Corporation | Detection of immobilized nucleic acid |
-
2007
- 2007-08-31 WO PCT/US2007/077383 patent/WO2008028127A1/en not_active Ceased
- 2007-08-31 JP JP2009526932A patent/JP2010502962A/ja active Pending
- 2007-08-31 EP EP07841717.7A patent/EP2062036A4/en not_active Withdrawn
- 2007-08-31 US US11/848,412 patent/US20080057557A1/en not_active Abandoned
-
2008
- 2008-10-28 US US12/259,823 patent/US20090145759A1/en not_active Abandoned
-
2012
- 2012-07-16 US US13/550,332 patent/US20130020199A1/en not_active Abandoned
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63222254A (ja) * | 1987-03-12 | 1988-09-16 | Kusano Hiroshi | 電気泳動装置用回収セル |
| JP2004155687A (ja) * | 2002-11-05 | 2004-06-03 | Forestry & Forest Products Research Institute | 等電点電気泳動法によるタンパク質溶出方法。 |
| JP2004290109A (ja) * | 2003-03-27 | 2004-10-21 | Matsushita Electric Ind Co Ltd | 生体物質回収装置 |
| JP2004294317A (ja) * | 2003-03-27 | 2004-10-21 | Matsushita Electric Ind Co Ltd | 生体物質回収装置 |
| JP2004309326A (ja) * | 2003-04-08 | 2004-11-04 | Yokogawa Electric Corp | 生体高分子の抽出方法および抽出装置 |
| JP2005241477A (ja) * | 2004-02-26 | 2005-09-08 | Japan Science & Technology Agency | 電気泳動用ゲルおよびその利用 |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014517314A (ja) * | 2011-06-16 | 2014-07-17 | ブリティッシュ コロンビア キャンサー エイジェンシー ブランチ | 核酸の自動サイズ選択 |
| US9952176B2 (en) | 2011-06-16 | 2018-04-24 | British Columbia Cancer Agency Branch | Automated size selection of nucleic acids |
| WO2020012805A1 (ja) * | 2018-07-12 | 2020-01-16 | 株式会社日立ハイテクノロジーズ | 電気泳動方法、電気泳動システム及び電気泳動ゲル |
| JP2020008513A (ja) * | 2018-07-12 | 2020-01-16 | 株式会社日立ハイテクノロジーズ | 電気泳動方法、電気泳動システム及び電気泳動ゲル |
| US11493477B2 (en) | 2018-07-12 | 2022-11-08 | Hitachi High-Tech Corporation | Electrophoresis method, electrophoresis system, and electrophoresis gel |
| US12467897B2 (en) | 2020-09-02 | 2025-11-11 | Hitachi High-Tech Corporation | Electrophoresis device and biological substance recovery method |
| JPWO2023002775A1 (enExample) * | 2021-07-19 | 2023-01-26 | ||
| WO2023002775A1 (ja) * | 2021-07-19 | 2023-01-26 | 株式会社ベックス | 電極および電極キット |
| JP7329295B2 (ja) | 2021-07-19 | 2023-08-18 | 株式会社ベックス | 電極および電極キット |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2062036A4 (en) | 2016-09-21 |
| WO2008028127A1 (en) | 2008-03-06 |
| US20090145759A1 (en) | 2009-06-11 |
| US20130020199A1 (en) | 2013-01-24 |
| EP2062036A1 (en) | 2009-05-27 |
| US20080057557A1 (en) | 2008-03-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010502962A (ja) | 電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置 | |
| US8361299B2 (en) | Multichannel preparative electrophoresis system | |
| EP1421373B1 (en) | Apparatus and method for separating an analyte | |
| US6562213B1 (en) | Electrophoresis apparatus for simultaneous loading of multiple samples | |
| US8926817B2 (en) | Programmable electrophoretic notch filter systems and methods | |
| AU2013329110B2 (en) | Side-eluting molecular fractionator | |
| JP2014013261A (ja) | 多重チャネルの分取用電気泳動システム | |
| US20110120867A1 (en) | Micro-channel chip for electrophoresis and method for electrophoresis | |
| EP2986978B1 (en) | Mini-gel comb | |
| US10710079B2 (en) | Electro-kinectic device for species exchange | |
| US20030087290A1 (en) | Bio-affinity porous matrix in microfluidic channels | |
| Kowalski et al. | High-resolution two-dimensional polyacrylamide gel electrophoresis: a tool for identification of polymorphic and modified linker histone components | |
| WO2020079211A1 (en) | Electrophoresis assembly | |
| JP2012058039A (ja) | キャピラリー電気泳動法による試料の分析方法、キャピラリー電気泳動装置及びキャピラリー電気泳動用チップ | |
| WO2025111055A1 (en) | Systems, devices, and methods for performing preparative electrophoresis | |
| KR20170000603U (ko) | 다중채널 파이펫 사용이 가능한 유전자 전기영동용 아가로즈 겔 | |
| WO2005083414A1 (ja) | 試料の分析方法ならびに分析装置及び導入方法 | |
| IL190930A (en) | Electrophoresis apparatus for simultaneous loading of multiple samples | |
| IL154643A (en) | Electrophoresis apparatus for simultaneous loading of multiple samples |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100426 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20100426 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110330 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110921 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20111215 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20111222 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20120517 |