JP2009529876A - 核酸を配列決定するための方法および手段 - Google Patents
核酸を配列決定するための方法および手段 Download PDFInfo
- Publication number
- JP2009529876A JP2009529876A JP2009500447A JP2009500447A JP2009529876A JP 2009529876 A JP2009529876 A JP 2009529876A JP 2009500447 A JP2009500447 A JP 2009500447A JP 2009500447 A JP2009500447 A JP 2009500447A JP 2009529876 A JP2009529876 A JP 2009529876A
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- Prior art keywords
- dna
- probe
- amplification
- hybridization
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US78173106P | 2006-03-14 | 2006-03-14 | |
PCT/US2007/006372 WO2007106509A2 (en) | 2006-03-14 | 2007-03-14 | Methods and means for nucleic acid sequencing |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2009529876A true JP2009529876A (ja) | 2009-08-27 |
Family
ID=38510066
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009500447A Pending JP2009529876A (ja) | 2006-03-14 | 2007-03-14 | 核酸を配列決定するための方法および手段 |
Country Status (6)
Country | Link |
---|---|
US (1) | US20100028873A1 (de) |
EP (1) | EP1999276A4 (de) |
JP (1) | JP2009529876A (de) |
CN (1) | CN101460633A (de) |
CA (1) | CA2647786A1 (de) |
WO (1) | WO2007106509A2 (de) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016531561A (ja) * | 2013-10-01 | 2016-10-13 | テクセル | 希少な微生物学的核酸の検出 |
JP2018072353A (ja) * | 2012-01-20 | 2018-05-10 | ジニア テクノロジーズ, インコーポレイテッド | ナノポアベースの分子検出および配列決定 |
Families Citing this family (57)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3492602A1 (de) | 2005-06-15 | 2019-06-05 | Complete Genomics, Inc. | Einzelne molekülarrays für genetische und chemische analyse |
WO2008027558A2 (en) | 2006-08-31 | 2008-03-06 | Codon Devices, Inc. | Iterative nucleic acid assembly using activation of vector-encoded traits |
US7910302B2 (en) | 2006-10-27 | 2011-03-22 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
US20090111706A1 (en) | 2006-11-09 | 2009-04-30 | Complete Genomics, Inc. | Selection of dna adaptor orientation by amplification |
WO2008134867A1 (en) * | 2007-05-04 | 2008-11-13 | Genizon Biosciences Inc. | Methods, kits, and systems for nucleic acid sequencing by hybridization |
US8278047B2 (en) | 2007-10-01 | 2012-10-02 | Nabsys, Inc. | Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment |
US8298768B2 (en) | 2007-11-29 | 2012-10-30 | Complete Genomics, Inc. | Efficient shotgun sequencing methods |
US8415099B2 (en) | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
WO2009097368A2 (en) | 2008-01-28 | 2009-08-06 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
US9650668B2 (en) | 2008-09-03 | 2017-05-16 | Nabsys 2.0 Llc | Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels |
US8262879B2 (en) | 2008-09-03 | 2012-09-11 | Nabsys, Inc. | Devices and methods for determining the length of biopolymers and distances between probes bound thereto |
US8882980B2 (en) | 2008-09-03 | 2014-11-11 | Nabsys, Inc. | Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels |
WO2010039991A2 (en) * | 2008-10-02 | 2010-04-08 | The Texas A&M University System | Method of generating informative dna templates for high-throughput sequencing applications |
US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
US8486630B2 (en) | 2008-11-07 | 2013-07-16 | Industrial Technology Research Institute | Methods for accurate sequence data and modified base position determination |
US20110269138A1 (en) * | 2008-12-30 | 2011-11-03 | Qiagen Hamburg Gmbh | Method for detecting methicillin-resistant staphylococcus aureus (mrsa) strains |
US20100331204A1 (en) * | 2009-02-13 | 2010-12-30 | Jeff Jeddeloh | Methods and systems for enrichment of target genomic sequences |
EP2396430B1 (de) * | 2009-02-16 | 2013-05-01 | Epicentre Technologies Corporation | Vorlagenunabhängige ligation von einzelstrang-dna |
US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
US20120015821A1 (en) * | 2009-09-09 | 2012-01-19 | Life Technologies Corporation | Methods of Generating Gene Specific Libraries |
WO2011103467A2 (en) | 2010-02-19 | 2011-08-25 | Life Technologies Corporation | Methods and systems for nucleic acid sequencing validation, calibration and normalization |
US8715933B2 (en) | 2010-09-27 | 2014-05-06 | Nabsys, Inc. | Assay methods using nicking endonucleases |
EP4039363A1 (de) | 2010-11-12 | 2022-08-10 | Gen9, Inc. | Proteinanordnungen und verfahren zu ihrer herstellung und verwendung |
EP3000883B8 (de) | 2010-11-12 | 2018-02-28 | Gen9, Inc. | Verfahren und vorrichtungen für nukleinsäuresynthese |
US8859201B2 (en) | 2010-11-16 | 2014-10-14 | Nabsys, Inc. | Methods for sequencing a biomolecule by detecting relative positions of hybridized probes |
CN102534811B (zh) * | 2010-12-16 | 2013-11-20 | 深圳华大基因科技服务有限公司 | 一种dna文库及其制备方法、一种dna测序方法和装置 |
CN102154188B (zh) * | 2010-12-22 | 2013-05-08 | 中国人民解放军第三军医大学 | 大肠杆菌DH5α的nfi基因敲除突变株及其制备方法和应用 |
US11274341B2 (en) | 2011-02-11 | 2022-03-15 | NABsys, 2.0 LLC | Assay methods using DNA binding proteins |
WO2012166647A1 (en) | 2011-05-27 | 2012-12-06 | Life Technologies Corporation | Methods for manipulating biomolecules |
US9752176B2 (en) * | 2011-06-15 | 2017-09-05 | Ginkgo Bioworks, Inc. | Methods for preparative in vitro cloning |
EP2748318B1 (de) | 2011-08-26 | 2015-11-04 | Gen9, Inc. | Zusammensetzungen und verfahren für hochqualitative anordnung von nukleinsäuren |
US10837879B2 (en) * | 2011-11-02 | 2020-11-17 | Complete Genomics, Inc. | Treatment for stabilizing nucleic acid arrays |
PT3363901T (pt) | 2012-02-17 | 2021-01-22 | Hutchinson Fred Cancer Res | Composições e métodos para identificar mutações com precisão |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
CN102634507B (zh) * | 2012-04-10 | 2014-09-17 | 张影频 | 多基因多区域的特异性捕获方法 |
CN102628082B (zh) * | 2012-04-10 | 2014-09-17 | 张影频 | 基于高通量测序技术进行核酸定性定量检测的方法 |
CN104603286B (zh) | 2012-04-24 | 2020-07-31 | Gen9股份有限公司 | 在体外克隆中分选核酸和多重制备物的方法 |
WO2014004393A1 (en) | 2012-06-25 | 2014-01-03 | Gen9, Inc. | Methods for nucleic acid assembly and high throughput sequencing |
JP6285929B2 (ja) * | 2012-07-17 | 2018-02-28 | カウンシル,インコーポレーテッド | 遺伝的変異を検出するためのシステムおよび方法 |
CN102839168A (zh) * | 2012-07-31 | 2012-12-26 | 深圳华大基因研究院 | 核酸探针及其制备方法和应用 |
US9914966B1 (en) | 2012-12-20 | 2018-03-13 | Nabsys 2.0 Llc | Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation |
US10294516B2 (en) | 2013-01-18 | 2019-05-21 | Nabsys 2.0 Llc | Enhanced probe binding |
EP2986738B1 (de) | 2013-04-17 | 2019-07-24 | Pioneer Hi-Bred International, Inc. | Verfahren zur charakterisierung der dna-sequenzzusammensetzung in einem genom |
WO2014183078A1 (en) | 2013-05-10 | 2014-11-13 | Foundation Medicine, Inc. | Analysis of genetic variants |
US11414695B2 (en) | 2013-05-29 | 2022-08-16 | Agilent Technologies, Inc. | Nucleic acid enrichment using Cas9 |
WO2016037361A1 (zh) * | 2014-09-12 | 2016-03-17 | 深圳华大基因科技有限公司 | 试剂盒及其在核酸测序中的用途 |
CN105624272B (zh) * | 2014-10-29 | 2019-08-09 | 深圳华大基因科技有限公司 | 基因组预定区域核酸测序文库的构建方法及装置 |
CN108699505A (zh) * | 2015-12-03 | 2018-10-23 | 安可济控股有限公司 | 用于形成连接产物的方法和组合物 |
CN105653896B (zh) * | 2016-01-22 | 2019-02-12 | 北京圣谷同创科技发展有限公司 | 高通量测序突变检测结果验证方法 |
WO2017222453A1 (en) | 2016-06-21 | 2017-12-28 | Hauling Thomas | Nucleic acid sequencing |
WO2018112806A1 (zh) * | 2016-12-21 | 2018-06-28 | 深圳华大智造科技有限公司 | 将线性测序文库转换为环状测序文库的方法 |
US11091791B2 (en) * | 2017-02-24 | 2021-08-17 | Mgi Tech Co., Ltd. | Methods for hybridization based hook ligation |
WO2018183942A1 (en) | 2017-03-31 | 2018-10-04 | Grail, Inc. | Improved library preparation and use thereof for sequencing-based error correction and/or variant identification |
CN110225979B (zh) * | 2017-05-23 | 2024-05-31 | 深圳华大基因股份有限公司 | 基于滚环扩增的基因组目标区域富集方法及其应用 |
CN111315895A (zh) * | 2017-09-14 | 2020-06-19 | 豪夫迈·罗氏有限公司 | 用于产生环状单链dna文库的新型方法 |
US11667968B2 (en) | 2021-05-27 | 2023-06-06 | New England Biolabs, Inc. | Fragmentation of DNA |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
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ZA929319B (en) * | 1991-12-11 | 1993-05-24 | Igen Inc | Method for exponential amplification of nucleic acid by a single unpaired primer. |
EP1098996A1 (de) * | 1998-07-20 | 2001-05-16 | Yale University | Verfahren zum nachweis von nukleinsäuren unter verwendung zielgerichteter ligation von zweiteiligen primern |
PT1165778E (pt) * | 1999-03-11 | 2007-01-31 | Glaxosmithkline Biolog Sa | Utilizações de polinucleótidos e polipéptidos casb618 |
AU2001241939A1 (en) * | 2000-02-28 | 2001-09-12 | Maxygen, Inc. | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
US20030203372A1 (en) * | 2000-12-08 | 2003-10-30 | Ward Neil Raymond | Analysis method |
US20040197791A1 (en) * | 2001-06-29 | 2004-10-07 | Makarov Vladimir L. | Methods of using nick translate libraries for snp analysis |
GB0207063D0 (en) * | 2002-03-26 | 2002-05-08 | Amersham Biosciences Uk Ltd | Immobilised probes |
WO2004009783A2 (en) * | 2002-07-24 | 2004-01-29 | New York University | Truncated rgr in t cell malignancy |
US20040224330A1 (en) * | 2003-01-15 | 2004-11-11 | Liyan He | Nucleic acid indexing |
EP1592810A2 (de) * | 2003-02-12 | 2005-11-09 | Genizon Svenska AB | Verfahren und vorrichtungen zur nukleinsäuresequenzierung |
GB2413796B (en) * | 2004-03-25 | 2006-03-29 | Global Genomics Ab | Methods and means for nucleic acid sequencing |
-
2007
- 2007-03-14 EP EP07753029A patent/EP1999276A4/de not_active Withdrawn
- 2007-03-14 US US12/293,013 patent/US20100028873A1/en not_active Abandoned
- 2007-03-14 WO PCT/US2007/006372 patent/WO2007106509A2/en active Application Filing
- 2007-03-14 CN CNA200780017676XA patent/CN101460633A/zh active Pending
- 2007-03-14 JP JP2009500447A patent/JP2009529876A/ja active Pending
- 2007-03-14 CA CA002647786A patent/CA2647786A1/en not_active Abandoned
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2018072353A (ja) * | 2012-01-20 | 2018-05-10 | ジニア テクノロジーズ, インコーポレイテッド | ナノポアベースの分子検出および配列決定 |
US11965210B2 (en) | 2012-01-20 | 2024-04-23 | Roche Sequencing Solutions, Inc. | Nanopore based molecular detection and sequencing |
JP2016531561A (ja) * | 2013-10-01 | 2016-10-13 | テクセル | 希少な微生物学的核酸の検出 |
Also Published As
Publication number | Publication date |
---|---|
EP1999276A4 (de) | 2010-08-04 |
US20100028873A1 (en) | 2010-02-04 |
CA2647786A1 (en) | 2007-09-20 |
WO2007106509A3 (en) | 2008-09-18 |
CN101460633A (zh) | 2009-06-17 |
WO2007106509A2 (en) | 2007-09-20 |
EP1999276A2 (de) | 2008-12-10 |
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Legal Events
Date | Code | Title | Description |
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A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20090702 |
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