JP2009512441A5 - - Google Patents
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- Publication number
- JP2009512441A5 JP2009512441A5 JP2008536632A JP2008536632A JP2009512441A5 JP 2009512441 A5 JP2009512441 A5 JP 2009512441A5 JP 2008536632 A JP2008536632 A JP 2008536632A JP 2008536632 A JP2008536632 A JP 2008536632A JP 2009512441 A5 JP2009512441 A5 JP 2009512441A5
- Authority
- JP
- Japan
- Prior art keywords
- open reading
- reading frame
- restriction enzyme
- polynucleotide
- interest
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091008146 restriction endonucleases Proteins 0.000 claims 49
- 108700026244 Open Reading Frames Proteins 0.000 claims 39
- 108091033319 polynucleotide Proteins 0.000 claims 35
- 239000002157 polynucleotide Substances 0.000 claims 35
- 102000040430 polynucleotide Human genes 0.000 claims 35
- 239000013598 vector Substances 0.000 claims 16
- 150000001413 amino acids Chemical class 0.000 claims 9
- 239000003153 chemical reaction reagent Substances 0.000 claims 8
- 229920001184 polypeptide Polymers 0.000 claims 8
- 102000004196 processed proteins & peptides Human genes 0.000 claims 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims 8
- 108091026890 Coding region Proteins 0.000 claims 7
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 230000004927 fusion Effects 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- -1 spacer amino acids Chemical class 0.000 claims 3
- 125000006850 spacer group Chemical group 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- 101710163270 Nuclease Proteins 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 2
- 108020001507 fusion proteins Proteins 0.000 claims 2
- 102000037865 fusion proteins Human genes 0.000 claims 2
- 108010042407 Endonucleases Proteins 0.000 claims 1
- 102000004533 Endonucleases Human genes 0.000 claims 1
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Natural products NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 230000029087 digestion Effects 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000013604 expression vector Substances 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 229940121649 protein inhibitor Drugs 0.000 claims 1
- 239000012268 protein inhibitor Substances 0.000 claims 1
- 230000026447 protein localization Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US72825905P | 2005-10-19 | 2005-10-19 | |
| US60/728,259 | 2005-10-19 | ||
| PCT/US2006/060065 WO2007076166A2 (en) | 2005-10-19 | 2006-10-19 | Methods of making modular fusion protein expression products |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009512441A JP2009512441A (ja) | 2009-03-26 |
| JP2009512441A5 true JP2009512441A5 (https=) | 2009-12-03 |
| JP5492415B2 JP5492415B2 (ja) | 2014-05-14 |
Family
ID=37963412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008536632A Expired - Fee Related JP5492415B2 (ja) | 2005-10-19 | 2006-10-19 | モジュラー融合タンパク質発現産物を作製する方法 |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US8153598B2 (https=) |
| EP (2) | EP2426196B1 (https=) |
| JP (1) | JP5492415B2 (https=) |
| KR (1) | KR101311435B1 (https=) |
| CN (2) | CN103215294B (https=) |
| AU (1) | AU2006330773B2 (https=) |
| CA (1) | CA2626666C (https=) |
| DK (2) | DK1948804T3 (https=) |
| ES (2) | ES2477285T3 (https=) |
| IL (1) | IL190884A (https=) |
| IN (1) | IN266806B (https=) |
| NZ (1) | NZ568106A (https=) |
| WO (1) | WO2007076166A2 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080050808A1 (en) * | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
| US7785871B2 (en) * | 2002-10-09 | 2010-08-31 | Intrexon Corporation | DNA cloning vector plasmids and methods for their use |
| JP2007537755A (ja) * | 2004-05-18 | 2007-12-27 | イントレクソン コーポレイション | Ｄｎａクローニングベクタープラスミドのダイナミックなベクター構築のための方法 |
| US8153598B2 (en) | 2005-10-19 | 2012-04-10 | Intrexon Corporation | PKD ligands and polynucleotides encoding PKD ligands |
| US7943732B2 (en) | 2006-06-05 | 2011-05-17 | Intrexon Corporation | AKT ligands and polynucleotides encoding AKT ligands |
| US8993263B2 (en) * | 2006-08-07 | 2015-03-31 | Intrexon Corporation | PKA ligands and polynucleotides encoding PKA ligands |
| US8999666B2 (en) * | 2006-08-09 | 2015-04-07 | Intrexon Corporation | PKC ligands and polynucleotides encoding PKC ligands |
| US8283445B2 (en) | 2006-11-13 | 2012-10-09 | Intrexon Corporation | Extracellular-signal-regulated-kinase (ERK) heteropolyligand polypeptide |
| US8729225B2 (en) | 2006-11-13 | 2014-05-20 | Intrexon Corporation | Glycogen synthase kinase heteropolyligand polypeptide |
| US7705122B2 (en) * | 2006-12-04 | 2010-04-27 | Intrexon Corporation | mTOR ligands and polynucleotides encoding mTOR ligands |
| EP2537924B1 (en) | 2007-03-27 | 2015-03-18 | Intrexon Corporation | MEK ligands and polynucleotides encoding mek ligands |
| WO2008137681A2 (en) * | 2007-05-02 | 2008-11-13 | Intrexon Corporation | Pp1 ligands |
| BRPI0906867A2 (pt) * | 2008-01-09 | 2019-09-24 | Intrexon Corp | inibidores terapêuticos da função pai-1 e métodos de uso dos mesmo |
| US9487808B2 (en) | 2010-09-24 | 2016-11-08 | Massachusetts Institute Of Technology | Rapid assembly of multiple arbitrary length DNA fragments |
| WO2015070210A1 (en) | 2013-11-11 | 2015-05-14 | Wake Forest University Health Sciences | Epha3 and multi-valent targeting of tumors |
| EP4282979A3 (en) * | 2015-09-01 | 2024-02-28 | The Regents of the University of California | Modular polypeptide libraries and methods of making and using same |
| WO2018017144A1 (en) | 2016-07-19 | 2018-01-25 | Brandeis University | Compositions and methods for identifying rna binding polypeptide targets |
| US20240409975A1 (en) * | 2021-09-27 | 2024-12-12 | Intergalactic Therapeutics, Inc. | Synthetic production of circular dna vectors |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661454A (en) | 1983-02-28 | 1987-04-28 | Collaborative Research, Inc. | GAL1 yeast promoter linked to non galactokinase gene |
| US4820642A (en) * | 1983-04-04 | 1989-04-11 | The Regents Of The University Of California | Amplified expression vector |
| US5061628A (en) | 1989-07-18 | 1991-10-29 | Rutgers University | Restriction endonuclease FseI |
| US5192676A (en) | 1991-02-05 | 1993-03-09 | New England Biolabs, Inc. | Type ii restriction endonuclease, asci, obtainable from arthrobacter species and a process for producing the same |
| US5223408A (en) | 1991-07-11 | 1993-06-29 | Genentech, Inc. | Method for making variant secreted proteins with altered properties |
| US5787104A (en) | 1995-01-19 | 1998-07-28 | Matsushita Electric Industrial Co., Ltd. | Semiconductor light emitting element and method for fabricating the same |
| US5919667A (en) | 1996-06-20 | 1999-07-06 | The Salk Institute For Biological Studies | Modular assembly retroviral vectors and uses thereof |
| US6248569B1 (en) | 1997-11-10 | 2001-06-19 | Brookhaven Science Associates | Method for introducing unidirectional nested deletions |
| US6096523A (en) | 1998-11-04 | 2000-08-01 | University Of Georgia Research Foundation | Transformation vector system |
| US6562624B2 (en) | 1999-03-17 | 2003-05-13 | Paradigm Genetics, Inc. | Methods and materials for the rapid and high volume production of a gene knock-out library in an organism |
| EP1165813A2 (en) | 1999-03-24 | 2002-01-02 | The Board Of Regents, The University Of Texas System | Linear and circular expression elements |
| US6245545B1 (en) | 1999-04-27 | 2001-06-12 | New England Biolabs, Inc. | Method for cloning and producing the SwaI restriction endonuclease |
| US20040031072A1 (en) * | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
| US6184000B1 (en) | 1999-07-23 | 2001-02-06 | The United States Of America As Represented By The Secretary Of Agriculture | System for the sequential, directional cloning of multiple DNA sequences |
| AU7752001A (en) | 2000-06-28 | 2002-01-08 | Sungene Gmbh And Co Kgaa | Binary vectors for improved transformation of plant systems |
| WO2002006834A2 (en) | 2000-07-19 | 2002-01-24 | Pointilliste, Inc. | Nested sorting and high throughput screening |
| FR2825103B1 (fr) | 2001-05-28 | 2003-09-19 | Genoway | Vecteurs de clonage pour recombinaison homologue et procede les utilisant |
| US6514737B1 (en) | 2001-08-20 | 2003-02-04 | New England Biolabs, Inc. | Method for cloning and expression of AsiSI restriction endonuclease and AsiSI methylase in E. coli |
| ZA200404575B (en) * | 2001-11-16 | 2006-03-29 | Heineken Tech Services | Method and apparatus for performng measurments on packaging for a fluid product |
| AU2003247962B2 (en) * | 2002-07-18 | 2008-06-12 | Monsanto Technology Llc | Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing |
| DE10238237A1 (de) * | 2002-08-21 | 2004-03-04 | Daimlerchrysler Ag | Kammerentleerungseinrichtung |
| CN1263860C (zh) | 2002-09-30 | 2006-07-12 | 华南农业大学 | 多基因载体的构建方法与应用 |
| US20080050808A1 (en) | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
| US7785871B2 (en) | 2002-10-09 | 2010-08-31 | Intrexon Corporation | DNA cloning vector plasmids and methods for their use |
| US7071295B2 (en) | 2002-12-02 | 2006-07-04 | Intrexon Corporation | Signal for targeting molecules to the sarco(endo)plasmic reticulum |
| TWI353991B (en) | 2003-05-06 | 2011-12-11 | Syntonix Pharmaceuticals Inc | Immunoglobulin chimeric monomer-dimer hybrids |
| US8293503B2 (en) * | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
| US7326567B2 (en) | 2003-11-12 | 2008-02-05 | Schering Corporation | Plasmid system for multigene expression |
| US20050227316A1 (en) | 2004-04-07 | 2005-10-13 | Kosan Biosciences, Inc. | Synthetic genes |
| JP2007537755A (ja) | 2004-05-18 | 2007-12-27 | イントレクソン コーポレイション | Ｄｎａクローニングベクタープラスミドのダイナミックなベクター構築のための方法 |
| US8153598B2 (en) | 2005-10-19 | 2012-04-10 | Intrexon Corporation | PKD ligands and polynucleotides encoding PKD ligands |
| WO2007048103A2 (en) | 2006-10-18 | 2007-04-26 | Intrexon Corporation | Pkd ligands and polynucleotides encoding pkd ligands |
| US7943732B2 (en) * | 2006-06-05 | 2011-05-17 | Intrexon Corporation | AKT ligands and polynucleotides encoding AKT ligands |
| US8993263B2 (en) * | 2006-08-07 | 2015-03-31 | Intrexon Corporation | PKA ligands and polynucleotides encoding PKA ligands |
| US8999666B2 (en) * | 2006-08-09 | 2015-04-07 | Intrexon Corporation | PKC ligands and polynucleotides encoding PKC ligands |
| US8729225B2 (en) * | 2006-11-13 | 2014-05-20 | Intrexon Corporation | Glycogen synthase kinase heteropolyligand polypeptide |
| US8283445B2 (en) * | 2006-11-13 | 2012-10-09 | Intrexon Corporation | Extracellular-signal-regulated-kinase (ERK) heteropolyligand polypeptide |
| US7705122B2 (en) * | 2006-12-04 | 2010-04-27 | Intrexon Corporation | mTOR ligands and polynucleotides encoding mTOR ligands |
| EP2537924B1 (en) | 2007-03-27 | 2015-03-18 | Intrexon Corporation | MEK ligands and polynucleotides encoding mek ligands |
-
2006
- 2006-10-18 US US12/090,462 patent/US8153598B2/en not_active Expired - Fee Related
- 2006-10-19 NZ NZ568106A patent/NZ568106A/xx not_active IP Right Cessation
- 2006-10-19 CN CN201310072134.XA patent/CN103215294B/zh not_active Expired - Fee Related
- 2006-10-19 ES ES11170207.2T patent/ES2477285T3/es active Active
- 2006-10-19 EP EP11170207.2A patent/EP2426196B1/en not_active Not-in-force
- 2006-10-19 CA CA2626666A patent/CA2626666C/en not_active Expired - Fee Related
- 2006-10-19 US US12/090,463 patent/US8603807B2/en not_active Expired - Fee Related
- 2006-10-19 KR KR1020087011967A patent/KR101311435B1/ko not_active Expired - Fee Related
- 2006-10-19 JP JP2008536632A patent/JP5492415B2/ja not_active Expired - Fee Related
- 2006-10-19 IN IN1806KON2008 patent/IN266806B/en unknown
- 2006-10-19 DK DK06848774.3T patent/DK1948804T3/da active
- 2006-10-19 WO PCT/US2006/060065 patent/WO2007076166A2/en not_active Ceased
- 2006-10-19 ES ES06848774T patent/ES2436277T3/es active Active
- 2006-10-19 EP EP06848774.3A patent/EP1948804B1/en not_active Not-in-force
- 2006-10-19 CN CNA2006800387906A patent/CN101395263A/zh active Pending
- 2006-10-19 DK DK11170207.2T patent/DK2426196T3/da active
- 2006-10-19 AU AU2006330773A patent/AU2006330773B2/en not_active Ceased
-
2008
- 2008-04-15 IL IL190884A patent/IL190884A/en not_active IP Right Cessation
-
2012
- 2012-01-11 US US13/348,515 patent/US8993531B2/en not_active Expired - Fee Related
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