DK1948804T3 - Fremgangsmåder til fremstilling af modulære fusionsproteinekspressionsprodukter - Google Patents

Fremgangsmåder til fremstilling af modulære fusionsproteinekspressionsprodukter

Info

Publication number
DK1948804T3
DK1948804T3 DK06848774.3T DK06848774T DK1948804T3 DK 1948804 T3 DK1948804 T3 DK 1948804T3 DK 06848774 T DK06848774 T DK 06848774T DK 1948804 T3 DK1948804 T3 DK 1948804T3
Authority
DK
Denmark
Prior art keywords
methods
fusion protein
protein expression
expression products
modular fusion
Prior art date
Application number
DK06848774.3T
Other languages
English (en)
Inventor
Thomas Reed
Original Assignee
Intrexon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intrexon Corp filed Critical Intrexon Corp
Application granted granted Critical
Publication of DK1948804T3 publication Critical patent/DK1948804T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4703—Inhibitors; Suppressors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK06848774.3T 2005-10-19 2006-10-19 Fremgangsmåder til fremstilling af modulære fusionsproteinekspressionsprodukter DK1948804T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72825905P 2005-10-19 2005-10-19
PCT/US2006/060065 WO2007076166A2 (en) 2005-10-19 2006-10-19 Methods of making modular fusion protein expression products

Publications (1)

Publication Number Publication Date
DK1948804T3 true DK1948804T3 (da) 2013-11-25

Family

ID=37963412

Family Applications (2)

Application Number Title Priority Date Filing Date
DK11170207.2T DK2426196T3 (da) 2005-10-19 2006-10-19 Fremgangsmåder til fremstilling af modulære fusionsproteinekspressionsprodukter
DK06848774.3T DK1948804T3 (da) 2005-10-19 2006-10-19 Fremgangsmåder til fremstilling af modulære fusionsproteinekspressionsprodukter

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK11170207.2T DK2426196T3 (da) 2005-10-19 2006-10-19 Fremgangsmåder til fremstilling af modulære fusionsproteinekspressionsprodukter

Country Status (13)

Country Link
US (3) US8153598B2 (da)
EP (2) EP1948804B1 (da)
JP (1) JP5492415B2 (da)
KR (1) KR101311435B1 (da)
CN (2) CN101395263A (da)
AU (1) AU2006330773B2 (da)
CA (1) CA2626666C (da)
DK (2) DK2426196T3 (da)
ES (2) ES2436277T3 (da)
IL (1) IL190884A (da)
IN (1) IN266806B (da)
NZ (1) NZ568106A (da)
WO (1) WO2007076166A2 (da)

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US7785871B2 (en) * 2002-10-09 2010-08-31 Intrexon Corporation DNA cloning vector plasmids and methods for their use
US20080050808A1 (en) * 2002-10-09 2008-02-28 Reed Thomas D DNA modular cloning vector plasmids and methods for their use
EP2484772B1 (en) 2004-05-18 2016-08-17 Intrexon Corporation Methods for dynamic vector assembly of DNA cloning vector plasmids
US8153598B2 (en) 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands
US7943732B2 (en) 2006-06-05 2011-05-17 Intrexon Corporation AKT ligands and polynucleotides encoding AKT ligands
US8993263B2 (en) * 2006-08-07 2015-03-31 Intrexon Corporation PKA ligands and polynucleotides encoding PKA ligands
US8999666B2 (en) * 2006-08-09 2015-04-07 Intrexon Corporation PKC ligands and polynucleotides encoding PKC ligands
US8729225B2 (en) 2006-11-13 2014-05-20 Intrexon Corporation Glycogen synthase kinase heteropolyligand polypeptide
US8283445B2 (en) 2006-11-13 2012-10-09 Intrexon Corporation Extracellular-signal-regulated-kinase (ERK) heteropolyligand polypeptide
US7705122B2 (en) 2006-12-04 2010-04-27 Intrexon Corporation mTOR ligands and polynucleotides encoding mTOR ligands
EP2537924B1 (en) 2007-03-27 2015-03-18 Intrexon Corporation MEK ligands and polynucleotides encoding mek ligands
KR101647149B1 (ko) * 2007-05-02 2016-08-09 인트렉손 코포레이션 Ｐｐ１ 리간드
AU2009204464B2 (en) 2008-01-09 2014-12-04 Intrexon Corporation Therapeutic inhibitors of PAI-1 function and methods of their use
US9487808B2 (en) 2010-09-24 2016-11-08 Massachusetts Institute Of Technology Rapid assembly of multiple arbitrary length DNA fragments
CA2930243A1 (en) 2013-11-11 2015-05-14 Wake Forest University Health Sciences Epha3 and multi-valent targeting of tumors
WO2017040694A2 (en) * 2015-09-01 2017-03-09 The Regents Of The University Of California Modular polypeptide libraries and methods of making and using same
EP3487998B1 (en) 2016-07-19 2023-07-12 Brandeis University Compositions and methods for identifying rna binding polypeptide targets
AU2022349697A1 (en) * 2021-09-27 2024-05-16 Intergalactic Therapeutics, Inc. Synthetic production of circular dna vectors

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US5061628A (en) * 1989-07-18 1991-10-29 Rutgers University Restriction endonuclease FseI
US5192676A (en) * 1991-02-05 1993-03-09 New England Biolabs, Inc. Type ii restriction endonuclease, asci, obtainable from arthrobacter species and a process for producing the same
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US7943732B2 (en) * 2006-06-05 2011-05-17 Intrexon Corporation AKT ligands and polynucleotides encoding AKT ligands
US8993263B2 (en) * 2006-08-07 2015-03-31 Intrexon Corporation PKA ligands and polynucleotides encoding PKA ligands
US8999666B2 (en) * 2006-08-09 2015-04-07 Intrexon Corporation PKC ligands and polynucleotides encoding PKC ligands
US8283445B2 (en) * 2006-11-13 2012-10-09 Intrexon Corporation Extracellular-signal-regulated-kinase (ERK) heteropolyligand polypeptide
US8729225B2 (en) * 2006-11-13 2014-05-20 Intrexon Corporation Glycogen synthase kinase heteropolyligand polypeptide
US7705122B2 (en) 2006-12-04 2010-04-27 Intrexon Corporation mTOR ligands and polynucleotides encoding mTOR ligands
EP2537924B1 (en) 2007-03-27 2015-03-18 Intrexon Corporation MEK ligands and polynucleotides encoding mek ligands

Also Published As

Publication number Publication date
US20090123973A1 (en) 2009-05-14
WO2007076166A2 (en) 2007-07-05
EP2426196B1 (en) 2014-04-02
CN103215294B (zh) 2015-09-09
US20090215173A1 (en) 2009-08-27
AU2006330773A1 (en) 2007-07-05
JP2009512441A (ja) 2009-03-26
DK2426196T3 (da) 2014-07-07
NZ568106A (en) 2012-08-31
CN103215294A (zh) 2013-07-24
CA2626666C (en) 2015-12-08
IN266806B (da) 2015-06-03
US20120195866A1 (en) 2012-08-02
IL190884A0 (en) 2008-11-03
WO2007076166A3 (en) 2008-11-20
ES2477285T3 (es) 2014-07-16
JP5492415B2 (ja) 2014-05-14
CN101395263A (zh) 2009-03-25
AU2006330773B2 (en) 2012-06-07
ES2436277T3 (es) 2013-12-30
US8993531B2 (en) 2015-03-31
EP1948804A2 (en) 2008-07-30
CA2626666A1 (en) 2007-07-05
EP1948804B1 (en) 2013-08-21
US8153598B2 (en) 2012-04-10
KR101311435B1 (ko) 2013-09-25
EP1948804A4 (en) 2009-05-13
US8603807B2 (en) 2013-12-10
KR20080080096A (ko) 2008-09-02
IL190884A (en) 2012-12-31
EP2426196A1 (en) 2012-03-07

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