CN103215294B - 制备模块融合蛋白表达产物的方法 - Google Patents

制备模块融合蛋白表达产物的方法 Download PDF

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CN103215294B
CN103215294B CN201310072134.XA CN201310072134A CN103215294B CN 103215294 B CN103215294 B CN 103215294B CN 201310072134 A CN201310072134 A CN 201310072134A CN 103215294 B CN103215294 B CN 103215294B
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CN103215294A (zh
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T·里德
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University of Pittsburgh
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    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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CN201310072134.XA 2005-10-19 2006-10-19 制备模块融合蛋白表达产物的方法 Expired - Fee Related CN103215294B (zh)

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US60/728,259 2005-10-19
CNA2006800387906A CN101395263A (zh) 2005-10-19 2006-10-19 制备模块融合蛋白表达产物的方法

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US (3) US8153598B2 (https=)
EP (2) EP2426196B1 (https=)
JP (1) JP5492415B2 (https=)
KR (1) KR101311435B1 (https=)
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US8153598B2 (en) 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands
US7943732B2 (en) 2006-06-05 2011-05-17 Intrexon Corporation AKT ligands and polynucleotides encoding AKT ligands
US8993263B2 (en) * 2006-08-07 2015-03-31 Intrexon Corporation PKA ligands and polynucleotides encoding PKA ligands
US8999666B2 (en) * 2006-08-09 2015-04-07 Intrexon Corporation PKC ligands and polynucleotides encoding PKC ligands
US8283445B2 (en) 2006-11-13 2012-10-09 Intrexon Corporation Extracellular-signal-regulated-kinase (ERK) heteropolyligand polypeptide
US8729225B2 (en) 2006-11-13 2014-05-20 Intrexon Corporation Glycogen synthase kinase heteropolyligand polypeptide
US7705122B2 (en) * 2006-12-04 2010-04-27 Intrexon Corporation mTOR ligands and polynucleotides encoding mTOR ligands
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WO2018017144A1 (en) 2016-07-19 2018-01-25 Brandeis University Compositions and methods for identifying rna binding polypeptide targets
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CA2626666A1 (en) 2007-07-05
DK2426196T3 (da) 2014-07-07
EP2426196B1 (en) 2014-04-02
US8603807B2 (en) 2013-12-10
US8153598B2 (en) 2012-04-10
ES2477285T3 (es) 2014-07-16
CA2626666C (en) 2015-12-08
ES2436277T3 (es) 2013-12-30
JP2009512441A (ja) 2009-03-26
JP5492415B2 (ja) 2014-05-14
KR20080080096A (ko) 2008-09-02
CN103215294A (zh) 2013-07-24
US20090123973A1 (en) 2009-05-14
IN266806B (https=) 2015-06-03
AU2006330773B2 (en) 2012-06-07
EP1948804B1 (en) 2013-08-21
IL190884A (en) 2012-12-31
NZ568106A (en) 2012-08-31
US8993531B2 (en) 2015-03-31
EP1948804A2 (en) 2008-07-30
CN101395263A (zh) 2009-03-25
WO2007076166A3 (en) 2008-11-20
WO2007076166A2 (en) 2007-07-05
AU2006330773A1 (en) 2007-07-05
IL190884A0 (en) 2008-11-03
US20120195866A1 (en) 2012-08-02
DK1948804T3 (da) 2013-11-25
KR101311435B1 (ko) 2013-09-25
US20090215173A1 (en) 2009-08-27
EP2426196A1 (en) 2012-03-07
EP1948804A4 (en) 2009-05-13

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