JP2009039074A5

JP2009039074A5 -

Info

Publication number: JP2009039074A5
Application number: JP2007209936A
Authority: JP
Filing date: 2007-08-10
Publication date: 2010-09-16
Anticipated expiration: 2027-08-10

Description

本発明の連続発酵によるタンパク質の製造方法の好ましい態様によれば、前記のタンパク質は酵素であり、その酵素は好ましくはデンプンまたはセルロースの糖化酵素、プロテアーゼあるいはリパーゼであり、より好ましくはα−アミラーゼあるいはグルコアミラーゼである。 According to a preferred embodiment of the method for producing a protein by continuous fermentation of the present invention, the protein is an enzyme, which is preferably a starch or cellulose saccharifying enzyme, a protease or a lipase, more preferably an α-amylase or Glucoamylase.

Claims

The fermentation broth of a microorganism having the ability to produce proteins is filtered through a separation membrane, the protein as a product is recovered from the filtrate, and the unfiltered liquid is retained or refluxed in the fermentation broth, and A method for producing a protein by continuous fermentation in which a fermentation raw material is added to the fermentation broth, wherein a porous membrane having pores having an average pore diameter of 0.01 μm or more and less than 1 μm is used as the separation membrane, A method for producing a protein by continuous fermentation, characterized by performing filtration treatment with a transmembrane pressure difference in the range of 0.1 to 20 kPa.

The pure water permeability coefficient of the porous membrane is 2 × 10 ⁻⁹ m ³ / m ² / s / pa or more and 6 × 10 ⁻⁷ m ³ / m ² / s / pa or less. A method for producing a protein.

The method for producing a protein by continuous fermentation according to claim 1 or 2, wherein the porous membrane has an average pore size of 0.01 µm or more and less than 0.2 µm, and a standard deviation of the average pore size is 0.1 µm or less.

The method for producing a protein by continuous fermentation according to any one of claims 1 to 3, wherein the membrane surface roughness of the porous membrane is 0.1 µm or less.

The method for producing a protein by continuous fermentation according to any one of claims 1 to 4, wherein the porous membrane is a porous membrane including a porous resin layer.

The method for producing a protein by continuous fermentation according to any one of claims 1 to 5, wherein the material of the porous membrane is a polyvinylidene fluoride resin.

The method for producing a protein by continuous fermentation according to any one of claims 1 to 6 , wherein the protein is an enzyme.

The method for producing a protein by continuous fermentation according to claim 7, wherein the enzyme is starch or cellulose saccharifying enzyme, protease or lipase.

The method for producing a protein by continuous fermentation according to claim 7 , wherein the enzyme is α-amylase or glucoamylase.