JP2008522585A - マイクロｒｎａ - Google Patents

マイクロｒｎａ Download PDF

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Publication number
JP2008522585A
JP2008522585A JP2007536777A JP2007536777A JP2008522585A JP 2008522585 A JP2008522585 A JP 2008522585A JP 2007536777 A JP2007536777 A JP 2007536777A JP 2007536777 A JP2007536777 A JP 2007536777A JP 2008522585 A JP2008522585 A JP 2008522585A
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Prior art keywords
mirna
nucleic acid
target sequence
plant
sequence
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Japanese (ja)
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JP2008522585A5 (enExample
Inventor
トボダ，ジョーズ・レイジェス
チャン，シューレン
敬 征矢野
チュア，ナム−ハイ
ニュ，クィー−ウェン
リン，シー・シュン
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Rockefeller University
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Rockefeller University
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Publication of JP2008522585A publication Critical patent/JP2008522585A/ja
Publication of JP2008522585A5 publication Critical patent/JP2008522585A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C12N2310/141—MicroRNAs, miRNAs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2007536777A 2004-10-12 2005-10-12 マイクロｒｎａ Withdrawn JP2008522585A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
USPCT/US2004/033379 2004-10-12
US67108905P 2005-04-14 2005-04-14
PCT/US2005/036373 WO2006044322A2 (en) 2004-10-12 2005-10-12 Micrornas

Publications (2)

Publication Number Publication Date
JP2008522585A true JP2008522585A (ja) 2008-07-03
JP2008522585A5 JP2008522585A5 (enExample) 2008-11-20

Family

ID=36203437

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007536777A Withdrawn JP2008522585A (ja) 2004-10-12 2005-10-12 マイクロｒｎａ

Country Status (10)

Country Link
US (2) US8975471B2 (enExample)
EP (1) EP1809748B1 (enExample)
JP (1) JP2008522585A (enExample)
CN (1) CN101076592B (enExample)
AR (1) AR050972A1 (enExample)
AU (1) AU2005295948A1 (enExample)
BR (1) BRPI0516874A (enExample)
CA (1) CA2583690C (enExample)
MX (1) MX2007004321A (enExample)
WO (1) WO2006044322A2 (enExample)

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EP2121921A4 (en) * 2006-12-13 2010-08-04 Idiverse Inc NEW, STRESSTOLERANZ TRANSMITTING GENES AND RNA MOLECULES
BRPI0806507B1 (pt) 2007-01-11 2017-05-02 Bayer Bioscience Nv genes quiméricos compreendendo um promotor específico para célula de fibra, uso do referido promotor e métodos para expressar um rna biologicamente ativo
EP2666865A3 (en) * 2007-01-18 2014-02-19 Plant Bioscience Limited (PBL) Methods and compostions for modulating the miRNA pathway
US8410334B2 (en) * 2007-02-20 2013-04-02 Monsanto Technology Llc Invertebrate microRNAs
WO2008143786A1 (en) * 2007-05-14 2008-11-27 The Rockefeller University Production of artificial micrornas using synthetic microrna precursors
WO2008150897A2 (en) 2007-05-31 2008-12-11 University Of Iowa Research Foundation Reduction of off-target rna interference toxicity
EP2192925A4 (en) * 2007-08-23 2013-04-03 Keren Pharmaceuticals TARGETING RNA WITH EXTERNAL GUIDE SEQUENCES
US8115055B2 (en) 2007-12-18 2012-02-14 E.I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
CN102016046A (zh) 2008-03-04 2011-04-13 华盛顿州立大学 用于区别性调节膜脂和种子油中脂肪酸不饱和性的组合物和方法
CN102076857B (zh) 2008-05-26 2015-08-19 拜尔作物科学公司 改变产纤维植物中纤维强度的方法和手段
US20110247094A1 (en) * 2008-10-08 2011-10-06 Edward Allen Transgenic Plants with Enhanced Agronomic Traits
US8309530B2 (en) 2009-02-04 2012-11-13 Washington State University Compositions and methods for modulating ghrelin-mediated conditions
EP2440666B1 (en) 2009-06-10 2017-03-01 Temasek Life Sciences Laboratory Limited Virus induced gene silencing (vigs) for functional analysis of genes in cotton
CN101710362B (zh) * 2009-12-10 2011-07-20 浙江大学 一种基于支持向量机的microRNA靶位点预测的方法
AU2011273004A1 (en) * 2010-06-30 2015-11-19 Basf Plant Science Company Gmbh Novel microRNA precursor and use thereof in regulation of target gene expression
CN101976296B (zh) * 2010-09-10 2012-05-23 浙江大学 结合基于下一代测序数据的植物microRNA靶位互作网络预测的方法
US20140259204A1 (en) * 2010-10-15 2014-09-11 The University Of Queensland Small viral rna molecules and uses thereof
US9181544B2 (en) 2011-02-12 2015-11-10 University Of Iowa Research Foundation Therapeutic compounds
UY33992A (es) * 2011-04-21 2012-09-28 Basf Plant Science Co Gmbh Métodos para modificar la biosintesis de la lignina y mejorar la digestibilidad
US20150089689A1 (en) * 2012-01-23 2015-03-26 E I Du Pont Nemours And Company Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthetic genes
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CN102703500B (zh) * 2012-05-30 2014-03-12 清华大学 利用植物病毒载体进行miRNA靶标模拟序列构建和表达的方法
US20140030792A1 (en) * 2012-07-23 2014-01-30 Radhakrishnan Rathnachalam Therapeutic Anti-Virus VLPS
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CN103088061B (zh) * 2013-01-15 2016-08-03 中国科学院微生物研究所 一种rna及载体在制备预防和/或治疗肝癌产品中的应用
WO2015099122A1 (ja) 2013-12-26 2015-07-02 学校法人東京医科大学 遺伝子発現制御のための人工ミミックｍｉＲＮＡおよびその用途
RU2697094C2 (ru) 2013-12-27 2019-08-12 Бонак Корпорейшн ИСКУССТВЕННАЯ мкРНК С СООТВЕТСТВИЕМ ДЛЯ КОНТРОЛЯ ЭКСПРЕССИИ ГЕНОВ И ЕЕ ПРИМЕНЕНИЕ
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CN104774837B (zh) * 2015-03-13 2018-02-02 浙江省医学科学院 9种植物微小核糖核苷酸及其应用
CN108064289A (zh) 2015-03-27 2018-05-22 株式会社博纳克 具有递送功能和基因表达调控能力的单链核酸分子
CN106167801B (zh) * 2016-09-30 2018-08-28 山东大学 大豆威廉姆斯82中NAC膜结合转录因子基因GmNTL1及其应用
WO2019103581A1 (ko) * 2017-11-27 2019-05-31 (주)프로스테믹스 이중 가닥의 올리고뉴클레오티드 및 그 제조 방법
CN111621588A (zh) * 2020-06-08 2020-09-04 华中农业大学 光亮果皮番茄品种选育的分子标记及其应用
CN112210555B (zh) * 2020-09-18 2022-02-22 大连理工大学 番茄抗晚疫病基因Sly-miR166b及其克隆方法与应用方法
CN114908089B (zh) * 2021-02-08 2024-03-15 上海细胞治疗集团有限公司 3’utr的构建方法和应用
CN114606236B (zh) * 2022-04-21 2024-01-05 南通大学 一种翘芪复方中药汤剂来源的微小核糖核酸及其制备方法和应用
CN115083516B (zh) * 2022-07-13 2023-03-21 北京先声医学检验实验室有限公司 一种基于靶向RNA测序技术检测基因融合的Panel设计和评估方法
CN117210463B (zh) * 2023-09-12 2024-03-19 扬州大学 一种与莲藕根状茎淀粉合成相关的miRNA及其应用
CN118516400B (zh) * 2024-07-24 2024-10-29 吉林农业大学 gma-miR169c基因在调控植物脂肪酸合成中的应用
CN120648688B (zh) * 2025-08-21 2025-11-07 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) 与植物抗病毒相关的miR159a及其在提高植物对病毒抗性中的应用

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Also Published As

Publication number Publication date
CN101076592A (zh) 2007-11-21
US20150159157A1 (en) 2015-06-11
CA2583690C (en) 2016-04-05
CA2583690A1 (en) 2006-04-27
WO2006044322A2 (en) 2006-04-27
EP1809748A4 (en) 2009-03-25
MX2007004321A (es) 2007-06-07
AR050972A1 (es) 2006-12-06
EP1809748A2 (en) 2007-07-25
US20060130176A1 (en) 2006-06-15
US8975471B2 (en) 2015-03-10
AU2005295948A1 (en) 2006-04-27
CN101076592B (zh) 2013-08-21
WO2006044322A3 (en) 2007-01-04
BRPI0516874A (pt) 2008-09-23
EP1809748B1 (en) 2012-12-26

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