CN101076592B - 微小rna - Google Patents

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Publication number
CN101076592B
CN101076592B CN2005800425477A CN200580042547A CN101076592B CN 101076592 B CN101076592 B CN 101076592B CN 2005800425477 A CN2005800425477 A CN 2005800425477A CN 200580042547 A CN200580042547 A CN 200580042547A CN 101076592 B CN101076592 B CN 101076592B
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mirna
sequence
plant
nucleic acid
seq
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CN101076592A (zh
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J·R·托伯达
张秀任
征矢野敬
蔡南海
牛其文
林诗舜
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Rockefeller University
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Rockefeller University
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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C12N2310/141—MicroRNAs, miRNAs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CN2005800425477A 2004-10-12 2005-10-12 微小rna Expired - Fee Related CN101076592B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
USPCT/US2004/033379 2004-10-12
USPCT/US04/33379 2004-10-12
US67108905P 2005-04-14 2005-04-14
US60/671,089 2005-04-14
PCT/US2005/036373 WO2006044322A2 (en) 2004-10-12 2005-10-12 Micrornas

Publications (2)

Publication Number Publication Date
CN101076592A CN101076592A (zh) 2007-11-21
CN101076592B true CN101076592B (zh) 2013-08-21

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CN2005800425477A Expired - Fee Related CN101076592B (zh) 2004-10-12 2005-10-12 微小rna

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US (2) US8975471B2 (enExample)
EP (1) EP1809748B1 (enExample)
JP (1) JP2008522585A (enExample)
CN (1) CN101076592B (enExample)
AR (1) AR050972A1 (enExample)
AU (1) AU2005295948A1 (enExample)
BR (1) BRPI0516874A (enExample)
CA (1) CA2583690C (enExample)
MX (1) MX2007004321A (enExample)
WO (1) WO2006044322A2 (enExample)

Cited By (1)

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CN102985545A (zh) * 2010-06-30 2013-03-20 巴斯夫植物科学有限公司 新型微小rna前体及其在调控靶基因表达中的用途

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US8237017B2 (en) 2006-05-12 2012-08-07 Bayer Cropscience Nv Stress-related microRNA molecules and uses thereof
AU2007290367B2 (en) 2006-08-31 2013-10-31 Monsanto Technology, Llc Phased small RNAs
US20080081330A1 (en) * 2006-09-28 2008-04-03 Helicos Biosciences Corporation Method and devices for analyzing small RNA molecules
US20080189805A1 (en) * 2006-12-13 2008-08-07 Serbin John J Novel genes and rna molecules that confer stress tolerance
WO2008083969A2 (en) 2007-01-11 2008-07-17 Bayer Bioscience N.V. Differential expression of subgenome specific alleles in cotton and uses thereof
CA2675954A1 (en) * 2007-01-18 2008-07-24 Plant Bioscience Limited (Pbl) Methods and compositions for modulating the mirna pathway
CN101646769A (zh) 2007-02-20 2010-02-10 孟山都技术公司 无脊椎动物微rna
US20080313773A1 (en) * 2007-05-14 2008-12-18 The Rockefeller University Production of artificial micrornas using synthetic microrna precursors
CA2691617C (en) 2007-05-31 2020-07-21 University Of Iowa Research Foundation Reduction of off-target rna interference toxicity
US20100292099A1 (en) * 2007-08-23 2010-11-18 Keren Pharmaceutical, Inc. Targeting of rna with external guide sequences
US8115055B2 (en) * 2007-12-18 2012-02-14 E.I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
WO2009111587A2 (en) 2008-03-04 2009-09-11 Washington State University Compositions and methods for differential regulation of fatty acid unsaturation in membrane lipids and seed oil
MX349774B (es) 2008-05-26 2017-08-10 Bayer Cropscience Nv Metodos y medios para modificar la resistencia de las fibras en plantas que producen fibras.
WO2010042575A1 (en) * 2008-10-08 2010-04-15 Monsanto Technology Llc Transgenic plants with enhanced agronomic traits
US8309530B2 (en) 2009-02-04 2012-11-13 Washington State University Compositions and methods for modulating ghrelin-mediated conditions
CN107129998A (zh) 2009-06-10 2017-09-05 淡马锡生命科学研究院有限公司 用于棉花中基因功能性分析的病毒诱导的基因沉默(vigs)
CN101710362B (zh) * 2009-12-10 2011-07-20 浙江大学 一种基于支持向量机的microRNA靶位点预测的方法
CN101976296B (zh) * 2010-09-10 2012-05-23 浙江大学 结合基于下一代测序数据的植物microRNA靶位互作网络预测的方法
CN103270158A (zh) * 2010-10-15 2013-08-28 昆士兰大学 小的病毒rna分子及其用途
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BR112014018009A2 (pt) * 2012-01-23 2018-06-26 Du Pont sequência de ácido nucleico isolada, construção recombinante, célula vegetal, método para reduzir a expressão de pelo menos um gene biossintético de ácido graxo vegetal, método para reduzir a expressão de dois ou mais genes biossintéticos de ácido graxo vegetal e planta ou semente transgênica
EP2825209B1 (en) 2012-03-14 2018-08-29 University of Central Florida Research Foundation, Inc. Neurofibromatoses therapeutic agents and screening for same
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CN102703500B (zh) * 2012-05-30 2014-03-12 清华大学 利用植物病毒载体进行miRNA靶标模拟序列构建和表达的方法
US20140030792A1 (en) * 2012-07-23 2014-01-30 Radhakrishnan Rathnachalam Therapeutic Anti-Virus VLPS
WO2014064704A2 (en) * 2012-10-28 2014-05-01 A.B. Seeds Ltd. Transgenic plants with modified sugar content and methods of generating same
CN103088061B (zh) * 2013-01-15 2016-08-03 中国科学院微生物研究所 一种rna及载体在制备预防和/或治疗肝癌产品中的应用
EP3088524A4 (en) 2013-12-26 2017-08-09 Tokyo Medical University Artificial mimic mirna for controlling gene expression, and use of same
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US9523093B2 (en) 2014-05-20 2016-12-20 University Of Iowa Research Foundation Huntington's disease therapeutic compounds
WO2016038511A1 (en) * 2014-09-10 2016-03-17 The New Zealand Institute For Plant And Food Research Limited Methods and materials for producing fruit of altered size
WO2016061232A2 (en) * 2014-10-14 2016-04-21 Texas Tech University System Multiplexed shrnas and uses thereof
CN107002062A (zh) 2014-10-22 2017-08-01 淡马锡生命科学研究院有限公司 来自依兰fruticosa 变种(Cananga odorata var. fruticosa)的萜烯合酶
AU2015368293B2 (en) 2014-12-27 2021-07-22 Bonac Corporation Naturally occuring miRNA for controlling gene expression, and use of same
CN104774837B (zh) * 2015-03-13 2018-02-02 浙江省医学科学院 9种植物微小核糖核苷酸及其应用
EP3276003B1 (en) 2015-03-27 2022-07-20 Bonac Corporation Single-chain nucleic acid molecule having delivery function and gene expression control ability
CN106167801B (zh) * 2016-09-30 2018-08-28 山东大学 大豆威廉姆斯82中NAC膜结合转录因子基因GmNTL1及其应用
KR102138495B1 (ko) * 2017-11-27 2020-07-28 (주)프로스테믹스 이중 가닥의 올리고뉴클레오티드 및 그 제조 방법
CN111621588A (zh) * 2020-06-08 2020-09-04 华中农业大学 光亮果皮番茄品种选育的分子标记及其应用
CN112210555B (zh) * 2020-09-18 2022-02-22 大连理工大学 番茄抗晚疫病基因Sly-miR166b及其克隆方法与应用方法
CN114908089B (zh) * 2021-02-08 2024-03-15 上海细胞治疗集团有限公司 3’utr的构建方法和应用
CN114606236B (zh) * 2022-04-21 2024-01-05 南通大学 一种翘芪复方中药汤剂来源的微小核糖核酸及其制备方法和应用
CN115083516B (zh) * 2022-07-13 2023-03-21 北京先声医学检验实验室有限公司 一种基于靶向RNA测序技术检测基因融合的Panel设计和评估方法
CN117210463B (zh) * 2023-09-12 2024-03-19 扬州大学 一种与莲藕根状茎淀粉合成相关的miRNA及其应用
CN118516400B (zh) * 2024-07-24 2024-10-29 吉林农业大学 gma-miR169c基因在调控植物脂肪酸合成中的应用
CN120648688B (zh) * 2025-08-21 2025-11-07 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) 与植物抗病毒相关的miR159a及其在提高植物对病毒抗性中的应用

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102985545A (zh) * 2010-06-30 2013-03-20 巴斯夫植物科学有限公司 新型微小rna前体及其在调控靶基因表达中的用途

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WO2006044322A2 (en) 2006-04-27
EP1809748A4 (en) 2009-03-25
BRPI0516874A (pt) 2008-09-23
WO2006044322A3 (en) 2007-01-04
US8975471B2 (en) 2015-03-10
AR050972A1 (es) 2006-12-06
MX2007004321A (es) 2007-06-07
JP2008522585A (ja) 2008-07-03
AU2005295948A1 (en) 2006-04-27
CA2583690C (en) 2016-04-05
CA2583690A1 (en) 2006-04-27
EP1809748B1 (en) 2012-12-26
US20060130176A1 (en) 2006-06-15
US20150159157A1 (en) 2015-06-11
CN101076592A (zh) 2007-11-21
EP1809748A2 (en) 2007-07-25

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