JP2008521104A5 - - Google Patents
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- JP2008521104A5 JP2008521104A5 JP2007541880A JP2007541880A JP2008521104A5 JP 2008521104 A5 JP2008521104 A5 JP 2008521104A5 JP 2007541880 A JP2007541880 A JP 2007541880A JP 2007541880 A JP2007541880 A JP 2007541880A JP 2008521104 A5 JP2008521104 A5 JP 2008521104A5
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- JP
- Japan
- Prior art keywords
- amplification
- value
- contents
- population
- amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000003321 amplification Effects 0.000 claims description 201
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 201
- 238000000034 method Methods 0.000 claims description 89
- 238000005259 measurement Methods 0.000 claims description 68
- 238000006243 chemical reaction Methods 0.000 claims description 43
- 230000008569 process Effects 0.000 claims description 38
- 238000002474 experimental method Methods 0.000 claims description 34
- 230000008859 change Effects 0.000 claims description 29
- 230000006870 function Effects 0.000 claims description 28
- 230000007423 decrease Effects 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 238000012545 processing Methods 0.000 claims description 12
- 238000011002 quantification Methods 0.000 claims description 12
- 230000003247 decreasing effect Effects 0.000 claims description 9
- 238000011282 treatment Methods 0.000 claims description 9
- 238000004590 computer program Methods 0.000 claims description 5
- 230000015654 memory Effects 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims description 2
- 238000003752 polymerase chain reaction Methods 0.000 claims description 2
- 239000013077 target material Substances 0.000 claims 1
- 239000000523 sample Substances 0.000 description 65
- 239000000047 product Substances 0.000 description 52
- 230000012010 growth Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000012886 linear function Methods 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 241000725303 Human immunodeficiency virus Species 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000010420 art technique Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 230000003936 working memory Effects 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0412471A FR2878350B1 (fr) | 2004-11-24 | 2004-11-24 | Procede installation et programme d'ordinateur pour estimer l'effectif initial d'une population d'acides nucleiques, notamment par pcr |
| FR0412471 | 2004-11-24 | ||
| US10/998,175 US7885767B2 (en) | 2004-11-24 | 2004-11-29 | Method, an installation, and a computer program for estimating the initial size of a population of nucleic acids, in particular by PCR |
| US10/998,175 | 2004-11-29 | ||
| PCT/EP2005/013021 WO2006048337A1 (en) | 2004-11-24 | 2005-11-18 | A method, an installation, and a computer program for estimating the initial size of a population of nucleic acids, in particular by pcr |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008521104A JP2008521104A (ja) | 2008-06-19 |
| JP2008521104A5 true JP2008521104A5 (https=) | 2011-02-17 |
| JP5014147B2 JP5014147B2 (ja) | 2012-08-29 |
Family
ID=34953944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007541880A Expired - Fee Related JP5014147B2 (ja) | 2004-11-24 | 2005-11-18 | 特にｐｃｒによる、核酸の個体群の初期個体量を見積もりするための方法、設備、およびコンピュータプログラム |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US7885767B2 (https=) |
| JP (1) | JP5014147B2 (https=) |
| FR (1) | FR2878350B1 (https=) |
| PT (1) | PT1700245E (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5744038B2 (ja) * | 2009-10-21 | 2015-07-01 | コーニンクレッカ フィリップス エヌ ヴェ | 増幅反応の解析ツール |
| EP2558971B1 (en) * | 2010-04-11 | 2021-02-17 | Life Technologies Corporation | SYSTEMS AND METHODS FOR MODEL-BASED qPCR |
-
2004
- 2004-11-24 FR FR0412471A patent/FR2878350B1/fr not_active Expired - Fee Related
- 2004-11-29 US US10/998,175 patent/US7885767B2/en not_active Expired - Fee Related
-
2005
- 2005-11-18 JP JP2007541880A patent/JP5014147B2/ja not_active Expired - Fee Related
- 2005-11-18 PT PT05815440T patent/PT1700245E/pt unknown
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