PT1700245E - Método, instalação e programa informático para estimar o tamanho inicial de uma população de ácidos nucleicos em particular por pcr - Google Patents
Método, instalação e programa informático para estimar o tamanho inicial de uma população de ácidos nucleicos em particular por pcr Download PDFInfo
- Publication number
- PT1700245E PT1700245E PT05815440T PT05815440T PT1700245E PT 1700245 E PT1700245 E PT 1700245E PT 05815440 T PT05815440 T PT 05815440T PT 05815440 T PT05815440 T PT 05815440T PT 1700245 E PT1700245 E PT 1700245E
- Authority
- PT
- Portugal
- Prior art keywords
- interest
- population
- sample
- index
- size
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 63
- 108020004707 nucleic acids Proteins 0.000 title claims description 17
- 150000007523 nucleic acids Chemical class 0.000 title claims description 17
- 102000039446 nucleic acids Human genes 0.000 title claims description 17
- 238000009434 installation Methods 0.000 title claims description 5
- 238000004590 computer program Methods 0.000 title claims description 4
- 238000005259 measurement Methods 0.000 claims description 107
- 230000003321 amplification Effects 0.000 claims description 74
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 74
- 230000007704 transition Effects 0.000 claims description 47
- 238000006243 chemical reaction Methods 0.000 claims description 42
- 230000006870 function Effects 0.000 claims description 25
- 230000003247 decreasing effect Effects 0.000 claims description 13
- 238000011002 quantification Methods 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 9
- 230000008859 change Effects 0.000 claims description 8
- 238000003753 real-time PCR Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 3
- 238000007670 refining Methods 0.000 claims description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 44
- 230000007423 decrease Effects 0.000 description 10
- 230000012010 growth Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000013459 approach Methods 0.000 description 3
- 230000002238 attenuated effect Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 241000725303 Human immunodeficiency virus Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000138 intercalating agent Substances 0.000 description 1
- 238000012886 linear function Methods 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0412471A FR2878350B1 (fr) | 2004-11-24 | 2004-11-24 | Procede installation et programme d'ordinateur pour estimer l'effectif initial d'une population d'acides nucleiques, notamment par pcr |
| US10/998,175 US7885767B2 (en) | 2004-11-24 | 2004-11-29 | Method, an installation, and a computer program for estimating the initial size of a population of nucleic acids, in particular by PCR |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT1700245E true PT1700245E (pt) | 2007-10-04 |
Family
ID=34953944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT05815440T PT1700245E (pt) | 2004-11-24 | 2005-11-18 | Método, instalação e programa informático para estimar o tamanho inicial de uma população de ácidos nucleicos em particular por pcr |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US7885767B2 (https=) |
| JP (1) | JP5014147B2 (https=) |
| FR (1) | FR2878350B1 (https=) |
| PT (1) | PT1700245E (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5744038B2 (ja) * | 2009-10-21 | 2015-07-01 | コーニンクレッカ フィリップス エヌ ヴェ | 増幅反応の解析ツール |
| EP2558971B1 (en) * | 2010-04-11 | 2021-02-17 | Life Technologies Corporation | SYSTEMS AND METHODS FOR MODEL-BASED qPCR |
-
2004
- 2004-11-24 FR FR0412471A patent/FR2878350B1/fr not_active Expired - Fee Related
- 2004-11-29 US US10/998,175 patent/US7885767B2/en not_active Expired - Fee Related
-
2005
- 2005-11-18 JP JP2007541880A patent/JP5014147B2/ja not_active Expired - Fee Related
- 2005-11-18 PT PT05815440T patent/PT1700245E/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FR2878350B1 (fr) | 2007-02-16 |
| FR2878350A1 (fr) | 2006-05-26 |
| JP5014147B2 (ja) | 2012-08-29 |
| US20060111883A1 (en) | 2006-05-25 |
| JP2008521104A (ja) | 2008-06-19 |
| US7885767B2 (en) | 2011-02-08 |
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