JP2008253263A5 - - Google Patents
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- Publication number
- JP2008253263A5 JP2008253263A5 JP2008094907A JP2008094907A JP2008253263A5 JP 2008253263 A5 JP2008253263 A5 JP 2008253263A5 JP 2008094907 A JP2008094907 A JP 2008094907A JP 2008094907 A JP2008094907 A JP 2008094907A JP 2008253263 A5 JP2008253263 A5 JP 2008253263A5
- Authority
- JP
- Japan
- Prior art keywords
- sample
- temperature
- target rna
- cdna
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920000160 (ribonucleotides)n+m Polymers 0.000 claims 3
- 229920002676 Complementary DNA Polymers 0.000 claims 2
- 239000002299 complementary DNA Substances 0.000 claims 2
- 238000003757 reverse transcription PCR Methods 0.000 claims 2
- 108010092799 EC 2.7.7.49 Proteins 0.000 claims 1
- 102000033147 ERVK-25 Human genes 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 1
- 230000001419 dependent Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
- 238000005382 thermal cycling Methods 0.000 claims 1
Claims (2)
- ａ）標的ＲＮＡを含むとされる試料を提供する工程
ｂ）該標的ＲＮＡをｃＤＮＡに逆転写するためおよび該ｃＤＮＡの少なくとも一部を増幅するために必要な全ての試薬を含む反応混合物を添加する工程
ｃ）２０℃〜６５℃の温度で０秒〜４０秒の時間間隔の間、該試料をインキュベートする工程
ｄ）該試料の温度が、少なくとも９０℃〜１００℃の第１の温度と５０℃〜７５℃の第２の温度との間で変化する、複数サイクルの熱サイクリングプロトコールに該試料を供する工程
を含む、標的ＲＮＡを増幅するためのワンステップＲＴ−ＰＣＲを実施する方法。 - 前記ワンステップＲＴ−ＰＣＲ反応が、少なくともＤＮＡ依存性ポリメラーゼ活性を含む熱安定性ポリメラーゼおよび少なくとも逆転写酵素の混合物によって触媒されることを特徴とする、請求項１〜４いずれか記載の方法。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07007173 | 2007-04-05 | ||
| EP07007173.3 | 2007-04-05 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008253263A JP2008253263A (ja) | 2008-10-23 |
| JP2008253263A5 true JP2008253263A5 (ja) | 2010-04-08 |
| JP5191041B2 JP5191041B2 (ja) | 2013-04-24 |
Family
ID=38453943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008094907A Active JP5191041B2 (ja) | 2007-04-05 | 2008-04-01 | 急速ワンステップｒｔ−ｐｃｒ |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US8119353B2 (ja) |
| EP (1) | EP1978109B1 (ja) |
| JP (1) | JP5191041B2 (ja) |
| ES (1) | ES2398984T3 (ja) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9175332B2 (en) * | 2010-07-29 | 2015-11-03 | Roche Molecular Systems, Inc. | Generic PCR |
| EP2625284B1 (en) | 2010-10-04 | 2015-01-28 | Roche Diagniostics GmbH | Method for cell lysis in a rt-pcr reaction buffer |
| WO2013101290A1 (en) * | 2011-12-29 | 2013-07-04 | Qiagen | Methods of using telomeres as markers for aging |
| EP3539666B1 (en) | 2012-05-24 | 2021-08-11 | University of Utah Research Foundation | Device for extreme pcr |
| WO2017079636A1 (en) * | 2015-11-05 | 2017-05-11 | University Of Utah Research Foundation | Extreme reverse transcription pcr |
| JP2018023360A (ja) * | 2016-07-29 | 2018-02-15 | セイコーエプソン株式会社 | 核酸増幅反応方法、試薬、および試薬の使用方法 |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| US20220064707A1 (en) * | 2019-01-17 | 2022-03-03 | Northwestern University | Rapid reverse transcription quantitative polymerase chain reaction |
| JP7180944B1 (ja) | 2020-12-25 | 2022-11-30 | 株式会社ニッポンジーン | 改変型ｄｎａポリメラーゼ |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5105599A (en) * | 1989-02-24 | 1992-04-21 | Highland Supply Corporation | Means for securing a decorative cover about a flower pot |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5561058A (en) | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
| US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| EP0506889B1 (en) | 1989-12-22 | 1997-04-02 | F. Hoffmann-La Roche AG | High temperature reverse transcriptases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| AU5142393A (en) | 1992-09-29 | 1994-04-26 | Cedars-Sinai Medical Center | One step rna and combined one step rna and dna polymerase chain reaction for detection of rare rna or rna and dna |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5876924A (en) * | 1994-06-22 | 1999-03-02 | Mount Sinai School Of Medicine | Nucleic acid amplification method hybridization signal amplification method (HSAM) |
| DE69739357D1 (de) | 1996-06-04 | 2009-05-28 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| WO1997046707A2 (en) | 1996-06-04 | 1997-12-11 | University Of Utah Research Foundation | System and method for monitoring for dna amplification by fluorescence |
| US7179590B2 (en) * | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
| JP4808365B2 (ja) * | 2001-03-02 | 2011-11-02 | ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション | Ｐｃｒ法 |
| US20030108898A1 (en) * | 2001-07-03 | 2003-06-12 | Paul Choppa | Method for detection, quantitation and breakpoint cluster region determination of fusion transcripts |
| CN100379882C (zh) * | 2003-01-17 | 2008-04-09 | 香港中文大学 | 作为妊娠相关病症的诊断标志物的循环mRNA |
-
2008
- 2008-04-01 JP JP2008094907A patent/JP5191041B2/ja active Active
- 2008-04-03 ES ES08006778T patent/ES2398984T3/es active Active
- 2008-04-03 US US12/061,700 patent/US8119353B2/en active Active
- 2008-04-03 EP EP08006778A patent/EP1978109B1/en active Active
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