JP2008212009A5 - - Google Patents
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- Publication number
- JP2008212009A5 JP2008212009A5 JP2007050748A JP2007050748A JP2008212009A5 JP 2008212009 A5 JP2008212009 A5 JP 2008212009A5 JP 2007050748 A JP2007050748 A JP 2007050748A JP 2007050748 A JP2007050748 A JP 2007050748A JP 2008212009 A5 JP2008212009 A5 JP 2008212009A5
- Authority
- JP
- Japan
- Prior art keywords
- biological sample
- dna
- sample pretreatment
- dna methylation
- pretreatment liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012472 biological sample Substances 0.000 claims 16
- 238000000034 method Methods 0.000 claims 9
- 230000007067 DNA methylation Effects 0.000 claims 8
- 239000003795 chemical substances by application Substances 0.000 claims 8
- 108020004414 DNA Proteins 0.000 claims 7
- 238000001514 detection method Methods 0.000 claims 6
- 239000007788 liquid Substances 0.000 claims 6
- 239000000523 sample Substances 0.000 claims 6
- 238000006243 chemical reaction Methods 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 5
- 239000000243 solution Substances 0.000 claims 5
- 210000004027 cell Anatomy 0.000 claims 4
- 108091005804 Peptidases Proteins 0.000 claims 3
- 239000004365 Protease Substances 0.000 claims 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 3
- 239000007864 aqueous solution Substances 0.000 claims 3
- 230000003196 chaotropic effect Effects 0.000 claims 3
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 3
- 239000004094 surface-active agent Substances 0.000 claims 3
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical group OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 2
- 239000011259 mixed solution Substances 0.000 claims 2
- 108091029523 CpG island Proteins 0.000 claims 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 230000009089 cytolysis Effects 0.000 claims 1
- 229960000789 guanidine hydrochloride Drugs 0.000 claims 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000028327 secretion Effects 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 210000004881 tumor cell Anatomy 0.000 claims 1
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007050748A JP2008212009A (ja) | 2007-02-28 | 2007-02-28 | Ｄｎａメチル化検出用試料の調製方法 |
| CNA2008100083371A CN101255476A (zh) | 2007-02-28 | 2008-02-26 | 用于制备检测dna甲基化试样的试样处理液和试剂盒 |
| US12/038,493 US20080206772A1 (en) | 2007-02-28 | 2008-02-27 | Sample treatment solution and reagent kit for preparing sample for detecting dna methylation |
| EP08152040A EP1964934A1 (en) | 2007-02-28 | 2008-02-28 | Sample treatment solution and reagent kit for preparing sample for detecting DNA methylation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007050748A JP2008212009A (ja) | 2007-02-28 | 2007-02-28 | Ｄｎａメチル化検出用試料の調製方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008212009A JP2008212009A (ja) | 2008-09-18 |
| JP2008212009A5 true JP2008212009A5 (https=) | 2008-11-06 |
Family
ID=39495902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007050748A Pending JP2008212009A (ja) | 2007-02-28 | 2007-02-28 | Ｄｎａメチル化検出用試料の調製方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20080206772A1 (https=) |
| EP (1) | EP1964934A1 (https=) |
| JP (1) | JP2008212009A (https=) |
| CN (1) | CN101255476A (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008136404A (ja) * | 2006-11-30 | 2008-06-19 | Sysmex Corp | Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法 |
| AU2008334901A1 (en) * | 2007-12-11 | 2009-06-18 | Epigenomics Ag | Methods and nucleic acids for analyses of lung carcinoma |
| WO2013067268A1 (en) | 2011-11-03 | 2013-05-10 | Tripath Imaging, Inc. | Methods and compositions for preparing samples for immunostaining |
| CN111235227A (zh) * | 2020-03-31 | 2020-06-05 | 西安天隆科技有限公司 | 一种游离dna提取及甲基化转化方法、试剂及试剂盒 |
| WO2022023753A1 (en) * | 2020-07-30 | 2022-02-03 | Cambridge Epigenetix Limited | Compositions and methods for nucleic acid analysis |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990001564A1 (en) * | 1988-08-09 | 1990-02-22 | Microprobe Corporation | Methods for multiple target analyses through nucleic acid hybridization |
| ATE374833T1 (de) | 1998-11-09 | 2007-10-15 | Eiken Chemical | Verfahren zur synthese von nukleinsäuren |
| WO2004086949A2 (en) * | 2003-03-25 | 2004-10-14 | John Wayne Cancer Institute | Dna markers for management of cancer |
| JP4166725B2 (ja) | 2003-07-31 | 2008-10-15 | 独立行政法人科学技術振興機構 | メチル化ｄｎａ検出方法 |
| WO2005054502A1 (en) * | 2003-12-02 | 2005-06-16 | Roche Diagnostics Gmbh | Improved method for bisulfite treatment |
| US20060234252A1 (en) * | 2004-07-02 | 2006-10-19 | Applera Corporation | Methods and kits for methylation detection |
| EP1632578A1 (en) * | 2004-09-03 | 2006-03-08 | Roche Diagnostics GmbH | DNA decontamination method |
-
2007
- 2007-02-28 JP JP2007050748A patent/JP2008212009A/ja active Pending
-
2008
- 2008-02-26 CN CNA2008100083371A patent/CN101255476A/zh active Pending
- 2008-02-27 US US12/038,493 patent/US20080206772A1/en not_active Abandoned
- 2008-02-28 EP EP08152040A patent/EP1964934A1/en not_active Withdrawn
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