JP2016501011A5 - - Google Patents
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- Publication number
- JP2016501011A5 JP2016501011A5 JP2015541121A JP2015541121A JP2016501011A5 JP 2016501011 A5 JP2016501011 A5 JP 2016501011A5 JP 2015541121 A JP2015541121 A JP 2015541121A JP 2015541121 A JP2015541121 A JP 2015541121A JP 2016501011 A5 JP2016501011 A5 JP 2016501011A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- solid support
- amplification
- cell sample
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004707 nucleic acids Proteins 0.000 claims 18
- 150000007523 nucleic acids Chemical class 0.000 claims 18
- 102000039446 nucleic acids Human genes 0.000 claims 18
- 238000000034 method Methods 0.000 claims 14
- 239000007787 solid Substances 0.000 claims 12
- 210000004027 cell Anatomy 0.000 claims 8
- 230000003321 amplification Effects 0.000 claims 6
- 239000003153 chemical reaction reagent Substances 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 108091092878 Microsatellite Proteins 0.000 claims 3
- 230000009089 cytolysis Effects 0.000 claims 3
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 claims 3
- 239000000243 solution Substances 0.000 claims 3
- 230000003196 chaotropic effect Effects 0.000 claims 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 claims 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims 1
- 239000005547 deoxyribonucleotide Substances 0.000 claims 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 1
- 239000003599 detergent Substances 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 210000003608 fece Anatomy 0.000 claims 1
- 229960000789 guanidine hydrochloride Drugs 0.000 claims 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 claims 1
- 210000004209 hair Anatomy 0.000 claims 1
- 238000003384 imaging method Methods 0.000 claims 1
- 238000011901 isothermal amplification Methods 0.000 claims 1
- 210000002751 lymph Anatomy 0.000 claims 1
- 210000003205 muscle Anatomy 0.000 claims 1
- 210000001236 prokaryotic cell Anatomy 0.000 claims 1
- 239000011535 reaction buffer Substances 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
- 238000003757 reverse transcription PCR Methods 0.000 claims 1
- 210000003296 saliva Anatomy 0.000 claims 1
- 210000000582 semen Anatomy 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 210000003491 skin Anatomy 0.000 claims 1
- 235000009518 sodium iodide Nutrition 0.000 claims 1
- 239000004094 surface-active agent Substances 0.000 claims 1
- 210000001179 synovial fluid Anatomy 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
- 210000001215 vagina Anatomy 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1220240.4A GB201220240D0 (en) | 2012-11-09 | 2012-11-09 | Methods for one step nucleic acid amplification of non-eluated samples |
| GB1220240.4 | 2012-11-09 | ||
| GBGB1301344.6A GB201301344D0 (en) | 2013-01-25 | 2013-01-25 | Methods for one step nucleic acid amplification of Non-Eluted samples |
| GB1301344.6 | 2013-01-25 | ||
| US201361789788P | 2013-03-15 | 2013-03-15 | |
| US61/789,788 | 2013-03-15 | ||
| PCT/EP2013/073189 WO2014072354A1 (en) | 2012-11-09 | 2013-11-06 | Methods for one step nucleic acid amplification of non-eluted samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016501011A JP2016501011A (ja) | 2016-01-18 |
| JP2016501011A5 true JP2016501011A5 (https=) | 2016-12-22 |
Family
ID=50684085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015541121A Pending JP2016501011A (ja) | 2012-11-09 | 2013-11-06 | 非溶出試料の一段階核酸増幅法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20150299770A1 (https=) |
| EP (1) | EP2917344B1 (https=) |
| JP (1) | JP2016501011A (https=) |
| CN (1) | CN104769111B (https=) |
| WO (1) | WO2014072354A1 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2971109B1 (en) | 2013-03-15 | 2018-10-24 | GE Healthcare UK Limited | Methods for one step nucleic acid amplification of non-eluted samples |
| US20150111214A1 (en) * | 2013-10-17 | 2015-04-23 | Life Technologies Corporation | Direct quantification of unprocessed nucleic acid samples |
| GB201411624D0 (en) * | 2014-06-30 | 2014-08-13 | Ge Healthcare Uk Ltd | Spatial molecular profiling of solid biological masses and profile storage |
| US9212388B1 (en) * | 2014-06-30 | 2015-12-15 | Life Technologies Corporation | Direct quantitative PCR absent minor groove binders |
| GB201515355D0 (en) * | 2015-08-28 | 2015-10-14 | Ge Healthcare Ltd | A method and kit for analyte detection |
| US10000742B2 (en) | 2015-11-19 | 2018-06-19 | General Electric Company | Device and method of collection for RNA viruses |
| GB201706680D0 (en) | 2017-04-27 | 2017-06-14 | Ge Healthcare Uk Ltd | Device and method for sample isolation |
| US20220016632A1 (en) * | 2018-12-04 | 2022-01-20 | IntegenX, Inc. | Controlling dna concentration for str analysis |
| KR20250047414A (ko) * | 2019-07-16 | 2025-04-03 | 멜리오랩스 인코포레이티드 | 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치 |
| US20240093284A1 (en) * | 2020-09-18 | 2024-03-21 | Avant Meats Company Limited | Quantification of cells embedded in a 3d scaffold |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5756126A (en) * | 1991-05-29 | 1998-05-26 | Flinders Technologies Pty. Ltd. | Dry solid medium for storage and analysis of genetic material |
| WO1996039813A1 (en) * | 1995-06-07 | 1996-12-19 | Flinders Technologies Pty. Ltd. | Dry solid medium for storage and analysis of genetic material |
| US5939259A (en) * | 1997-04-09 | 1999-08-17 | Schleicher & Schuell, Inc. | Methods and devices for collecting and storing clinical samples for genetic analysis |
| US6746841B1 (en) * | 1999-04-14 | 2004-06-08 | Whatman Inc. | FTA- coated media for use as a molecular diagnostic tool |
| US20060160078A1 (en) * | 2002-07-12 | 2006-07-20 | Cardy Donald L N | Lateral flow assay device and method |
| JP2008099622A (ja) * | 2006-10-20 | 2008-05-01 | Japan Health Science Foundation | 体液中の遺伝子増幅方法 |
| EP2294225B8 (en) * | 2008-06-30 | 2015-02-18 | Life Technologies Corporation | Method for direct amplification from crude nucleic acid samples |
| US9725754B2 (en) * | 2010-07-29 | 2017-08-08 | Sean F. Boyle | Generic sample preparation |
| EP2971109B1 (en) * | 2013-03-15 | 2018-10-24 | GE Healthcare UK Limited | Methods for one step nucleic acid amplification of non-eluted samples |
-
2013
- 2013-11-06 CN CN201380058557.4A patent/CN104769111B/zh active Active
- 2013-11-06 US US14/441,569 patent/US20150299770A1/en active Pending
- 2013-11-06 EP EP13786287.6A patent/EP2917344B1/en active Active
- 2013-11-06 WO PCT/EP2013/073189 patent/WO2014072354A1/en not_active Ceased
- 2013-11-06 JP JP2015541121A patent/JP2016501011A/ja active Pending
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