JP2008029329A5 - - Google Patents
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- JP2008029329A5 JP2008029329A5 JP2007163859A JP2007163859A JP2008029329A5 JP 2008029329 A5 JP2008029329 A5 JP 2008029329A5 JP 2007163859 A JP2007163859 A JP 2007163859A JP 2007163859 A JP2007163859 A JP 2007163859A JP 2008029329 A5 JP2008029329 A5 JP 2008029329A5
- Authority
- JP
- Japan
- Prior art keywords
- gene
- yeast
- xenopus laevis
- derived
- ldh gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108090000623 proteins and genes Proteins 0.000 claims description 76
- 241000269368 Xenopus laevis Species 0.000 claims description 62
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 49
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 47
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 claims description 20
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims description 16
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims description 16
- 210000000349 chromosome Anatomy 0.000 claims description 14
- 101150050255 PDC1 gene Proteins 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 9
- 230000014509 gene expression Effects 0.000 claims description 7
- 241000235070 Saccharomyces Species 0.000 claims description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 150000007523 nucleic acids Chemical group 0.000 claims description 4
- 101710195736 Pyruvate decarboxylase 1 Proteins 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 description 24
- 238000003752 polymerase chain reaction Methods 0.000 description 22
- 239000012634 fragment Substances 0.000 description 18
- 108020004414 DNA Proteins 0.000 description 15
- 239000013604 expression vector Substances 0.000 description 13
- 238000011144 upstream manufacturing Methods 0.000 description 8
- 101100480861 Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) tdh gene Proteins 0.000 description 6
- 101100447466 Candida albicans (strain WO-1) TDH1 gene Proteins 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 101150088047 tdh3 gene Proteins 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 5
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 101100174613 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TDH3 gene Proteins 0.000 description 5
- 241000269370 Xenopus <genus> Species 0.000 description 5
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
- 238000002744 homologous recombination Methods 0.000 description 5
- 230000006801 homologous recombination Effects 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 238000012790 confirmation Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 3
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 3
- 238000007400 DNA extraction Methods 0.000 description 3
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 3
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 3
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 101100351264 Candida albicans (strain SC5314 / ATCC MYA-2876) PDC11 gene Proteins 0.000 description 2
- 101710088194 Dehydrogenase Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 206010071602 Genetic polymorphism Diseases 0.000 description 2
- 101100028920 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cfp gene Proteins 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 101150050575 URA3 gene Proteins 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000002708 random mutagenesis Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 229940035893 uracil Drugs 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 108090000489 Carboxy-Lyases Proteins 0.000 description 1
- 108010075031 Cytochromes c Proteins 0.000 description 1
- LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 241000235649 Kluyveromyces Species 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 101001004672 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Probable L-lactate dehydrogenase Proteins 0.000 description 1
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- ZKLLSNQJRLJIGT-UYFOZJQFSA-N keto-D-fructose 1-phosphate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)COP(O)(O)=O ZKLLSNQJRLJIGT-UYFOZJQFSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007163859A JP5320692B2 (ja) | 2006-06-28 | 2007-06-21 | 酵母及びｌ−乳酸の製造方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006178048 | 2006-06-28 | ||
| JP2006178048 | 2006-06-28 | ||
| JP2007163859A JP5320692B2 (ja) | 2006-06-28 | 2007-06-21 | 酵母及びｌ−乳酸の製造方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008029329A JP2008029329A (ja) | 2008-02-14 |
| JP2008029329A5 true JP2008029329A5 (enExample) | 2010-07-22 |
| JP5320692B2 JP5320692B2 (ja) | 2013-10-23 |
Family
ID=39119381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007163859A Expired - Fee Related JP5320692B2 (ja) | 2006-06-28 | 2007-06-21 | 酵母及びｌ−乳酸の製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5320692B2 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0618394A2 (pt) | 2005-10-14 | 2011-08-30 | Toray Industries | levedura e métodos de produção de ácido l-lático |
| JP5992135B2 (ja) | 2008-02-04 | 2016-09-14 | 東レ株式会社 | 連続発酵による乳酸の製造方法 |
| WO2010074222A1 (ja) | 2008-12-26 | 2010-07-01 | 東レ株式会社 | 乳酸およびポリ乳酸の製造方法 |
| AU2010255000A1 (en) | 2009-06-03 | 2011-12-15 | Toray Industries, Inc. | Polypeptide having D-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of D-lactic acid |
| WO2012147903A1 (ja) * | 2011-04-28 | 2012-11-01 | 東レ株式会社 | 乳酸生産酵母変異株および乳酸の製造方法 |
| US9150835B2 (en) * | 2011-06-24 | 2015-10-06 | Samsung Electronics Co., Ltd. | Modified microorganism for highly efficient production of lactic acid |
| WO2013015212A1 (ja) | 2011-07-22 | 2013-01-31 | 東レ株式会社 | 有機酸の製造方法 |
| US9593350B2 (en) | 2014-02-14 | 2017-03-14 | Samsung Electronics Co., Ltd. | Lactate dehydrogenase mutant, polynucleotide coding for the mutant, yeast cell including the polynucleotide, method of preparing the mutant, and method of producing the lactate using the same |
| CN113430127B (zh) * | 2021-07-07 | 2024-01-12 | 江南大学 | 一种产l-乳酸的重组菌及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1294728B1 (it) * | 1997-09-12 | 1999-04-12 | Biopolo S C A R L | Ceppi di lievito per la riproduzione di acido lattico |
| JP2003259878A (ja) * | 2002-03-11 | 2003-09-16 | Toyota Central Res & Dev Lab Inc | 乳酸脱水素酵素をコードするｄｎａおよびその利用 |
| CN101157930A (zh) * | 2002-05-30 | 2008-04-09 | 卡吉尔道有限责任公司 | 在酵母中生产l-乳酸的方法与材料 |
-
2007
- 2007-06-21 JP JP2007163859A patent/JP5320692B2/ja not_active Expired - Fee Related
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