JP2007332080A - IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 - Google Patents
IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 Download PDFInfo
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Abstract
【解決手段】 IgM型抗体含有液に前処理を施し尿酸ナトリウム結晶に高親和性を有する夾雑成分を除去した後、尿酸ナトリウム結晶と接触させると、IgM型抗体が尿酸ナトリウム結晶に選択的に結合するため、簡便に高純度かつ高活性なIgM型抗体を精製することができる。
【選択図】 なし
Description
のみに限定されるものではない。
全血清をリポソーバー(株式会社カネカ製)を充填したLDL除去カラムに通し、LDL除去画分と1M塩化ナトリウム溶液で溶出したLDL画分を得た。LDL除去画分をさらに微顆粒性セルロースの陰イオン交換カラムDE52(Whatman)に通しIgG画分とIgG除去画分を得た。またLDL除去画分にZymosanを加え37℃で30分インキュベートして補体消費画分を得た。
マウスモノクローナル抗体を以下のようにして作成した。ヒト尿細管分泌尿蛋白質に対するIgM型(UP509、UP599)およびIgG1型マウスモノクローナル抗体(UP720)を産生するハイブリドーマ(日本泌尿器科学会雑誌.87巻、1120頁、1996年)を、牛胎児血清を10%含む培養液(RPMI1640)で培養し上清を採取した。
IgM型抗体を産生する3種類のハイブリドーマ(UP70、UP509、UP599)をBALB/cマウスの腹腔内に注射して貯留した腹水を採取した。
Claims (8)
- IgM型抗体含有液を尿酸ナトリウム結晶と接触させることによりIgM型抗体を尿酸ナトリウム結晶に選択的に結合させ、その後にIgM型抗体を尿酸ナトリウム結晶から解離、回収することを特徴とするIgM型抗体の分離精製方法。
- カチオン交換能を有する吸着材と接触させることを含む請求項1記載のIgM型抗体の分離精製方法。
- カチオン交換能を有する吸着材としてアニオン性化合物を固定してなる吸着材を用いる請求項1または2記載のIgM型抗体の分離精製方法。
- アニオン性化合物としてデキストラン硫酸を固定してなる吸着材を用いることを特徴とする請求項3記載のIgM型抗体の分離精製方法。
- アニオン交換能を有する吸着材と接触させることを含む請求項1乃至4記載のIgM型抗体の分離精製方法。
- 請求項1乃至5記載の方法により、分離精製されたIgM型抗体。
- IgM型抗体含有液を尿酸ナトリウム結晶と接触させることにより、尿酸ナトリウム結晶にIgM型抗体を結合させた、IgM型抗体が認識する抗原の吸着材。
- IgM型抗体含有液を尿酸ナトリウム結晶と接触させることによりIgM型抗体を尿酸ナトリウム結晶に選択的に結合させ、その後にIgM型抗体を尿酸ナトリウム結晶から解離、回収したIgM型抗体を結合させた、IgM型抗体が認識する抗原の吸着材。
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JP2006166542A JP5089924B2 (ja) | 2006-06-15 | 2006-06-15 | IgM型抗体の精製方法、IgM型抗体認識抗原の吸着材 |
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JP2007332080A true JP2007332080A (ja) | 2007-12-27 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010241731A (ja) * | 2009-04-07 | 2010-10-28 | Kitasato Institute | 抗dcdモノクローナル抗体 |
JP2010284607A (ja) * | 2009-06-12 | 2010-12-24 | Toshiba Corp | リン吸着材、及びリン回収システム |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS559036A (en) * | 1978-07-04 | 1980-01-22 | Green Cross Corp:The | Purification of igm |
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2006
- 2006-06-15 JP JP2006166542A patent/JP5089924B2/ja active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS559036A (en) * | 1978-07-04 | 1980-01-22 | Green Cross Corp:The | Purification of igm |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010241731A (ja) * | 2009-04-07 | 2010-10-28 | Kitasato Institute | 抗dcdモノクローナル抗体 |
JP2010284607A (ja) * | 2009-06-12 | 2010-12-24 | Toshiba Corp | リン吸着材、及びリン回収システム |
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