JP2007275069A - Pca3タンパク質、pca3遺伝子、及びこれらの用途 - Google Patents
Pca3タンパク質、pca3遺伝子、及びこれらの用途 Download PDFInfo
- Publication number
- JP2007275069A JP2007275069A JP2007125170A JP2007125170A JP2007275069A JP 2007275069 A JP2007275069 A JP 2007275069A JP 2007125170 A JP2007125170 A JP 2007125170A JP 2007125170 A JP2007125170 A JP 2007125170A JP 2007275069 A JP2007275069 A JP 2007275069A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- pca3
- sequence
- acid molecule
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091033411 PCA3 Proteins 0.000 title claims abstract description 325
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 171
- 102000004169 proteins and genes Human genes 0.000 title abstract description 83
- 238000000034 method Methods 0.000 claims abstract description 154
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 133
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 123
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 123
- 239000000523 sample Substances 0.000 claims abstract description 75
- 206010060862 Prostate cancer Diseases 0.000 claims abstract description 74
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims abstract description 74
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 210000004027 cell Anatomy 0.000 claims description 152
- 108020004414 DNA Proteins 0.000 claims description 62
- 210000001519 tissue Anatomy 0.000 claims description 47
- 210000002307 prostate Anatomy 0.000 claims description 32
- 239000013598 vector Substances 0.000 claims description 29
- 108700024394 Exon Proteins 0.000 claims description 23
- 108020004999 messenger RNA Proteins 0.000 claims description 21
- 125000003729 nucleotide group Chemical group 0.000 claims description 20
- 239000002773 nucleotide Substances 0.000 claims description 19
- 230000000295 complement effect Effects 0.000 claims description 17
- 238000009396 hybridization Methods 0.000 claims description 16
- 238000013518 transcription Methods 0.000 claims description 14
- 230000035897 transcription Effects 0.000 claims description 13
- 239000000284 extract Substances 0.000 claims description 11
- 210000004369 blood Anatomy 0.000 claims description 10
- 239000008280 blood Substances 0.000 claims description 10
- 239000012472 biological sample Substances 0.000 claims description 9
- 210000002966 serum Anatomy 0.000 claims description 9
- 210000001124 body fluid Anatomy 0.000 claims description 8
- 239000010839 body fluid Substances 0.000 claims description 8
- 210000000056 organ Anatomy 0.000 claims description 5
- 238000009007 Diagnostic Kit Methods 0.000 claims description 4
- 210000005267 prostate cell Anatomy 0.000 claims description 3
- 210000001550 testis Anatomy 0.000 claims description 3
- 210000002700 urine Anatomy 0.000 claims description 3
- PBLQSFOIWOTFNY-UHFFFAOYSA-N 3-methylbut-2-enyl 4-methoxy-8-(3-methylbut-2-enoxy)quinoline-2-carboxylate Chemical compound C1=CC=C2C(OC)=CC(C(=O)OCC=C(C)C)=NC2=C1OCC=C(C)C PBLQSFOIWOTFNY-UHFFFAOYSA-N 0.000 claims description 2
- 241000972773 Aulopiformes Species 0.000 claims description 2
- 210000001367 artery Anatomy 0.000 claims description 2
- 210000004556 brain Anatomy 0.000 claims description 2
- 210000001198 duodenum Anatomy 0.000 claims description 2
- 210000002216 heart Anatomy 0.000 claims description 2
- 210000004185 liver Anatomy 0.000 claims description 2
- 210000001672 ovary Anatomy 0.000 claims description 2
- 210000002826 placenta Anatomy 0.000 claims description 2
- 210000002381 plasma Anatomy 0.000 claims description 2
- 235000019515 salmon Nutrition 0.000 claims description 2
- 210000001625 seminal vesicle Anatomy 0.000 claims description 2
- 210000002027 skeletal muscle Anatomy 0.000 claims description 2
- 210000003491 skin Anatomy 0.000 claims description 2
- 210000000278 spinal cord Anatomy 0.000 claims description 2
- 210000000952 spleen Anatomy 0.000 claims description 2
- 201000010653 vesiculitis Diseases 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 109
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 89
- 229920001184 polypeptide Polymers 0.000 abstract description 78
- 241001465754 Metazoa Species 0.000 abstract description 44
- 108010072866 Prostate-Specific Antigen Proteins 0.000 abstract description 36
- 102000007066 Prostate-Specific Antigen Human genes 0.000 abstract description 36
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract description 23
- 239000002853 nucleic acid probe Substances 0.000 abstract description 23
- 238000011282 treatment Methods 0.000 abstract description 19
- 206010028980 Neoplasm Diseases 0.000 abstract description 18
- 201000011510 cancer Diseases 0.000 abstract description 13
- 241000124008 Mammalia Species 0.000 abstract description 8
- 230000009870 specific binding Effects 0.000 abstract description 4
- 238000004166 bioassay Methods 0.000 abstract description 3
- 108091005461 Nucleic proteins Proteins 0.000 abstract description 2
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 74
- 230000014509 gene expression Effects 0.000 description 57
- 238000010804 cDNA synthesis Methods 0.000 description 56
- 108020004635 Complementary DNA Proteins 0.000 description 55
- 239000002299 complementary DNA Substances 0.000 description 55
- 239000012634 fragment Substances 0.000 description 49
- 108091028043 Nucleic acid sequence Proteins 0.000 description 47
- 108700019146 Transgenes Proteins 0.000 description 44
- 125000003275 alpha amino acid group Chemical group 0.000 description 33
- 125000000539 amino acid group Chemical group 0.000 description 31
- 230000000694 effects Effects 0.000 description 26
- 239000000126 substance Substances 0.000 description 25
- 201000010099 disease Diseases 0.000 description 24
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 24
- 230000009261 transgenic effect Effects 0.000 description 24
- 230000035772 mutation Effects 0.000 description 23
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 22
- 230000027455 binding Effects 0.000 description 18
- 239000003153 chemical reaction reagent Substances 0.000 description 18
- 230000006870 function Effects 0.000 description 17
- 238000012216 screening Methods 0.000 description 17
- 238000006467 substitution reaction Methods 0.000 description 17
- 230000001225 therapeutic effect Effects 0.000 description 17
- 238000004458 analytical method Methods 0.000 description 16
- 239000003814 drug Substances 0.000 description 16
- 239000000047 product Substances 0.000 description 16
- 102000004190 Enzymes Human genes 0.000 description 15
- 108090000790 Enzymes Proteins 0.000 description 15
- 239000003795 chemical substances by application Substances 0.000 description 15
- 229940088598 enzyme Drugs 0.000 description 15
- 239000003446 ligand Substances 0.000 description 15
- 239000003550 marker Substances 0.000 description 15
- 108091026890 Coding region Proteins 0.000 description 14
- 238000003556 assay Methods 0.000 description 14
- 238000003752 polymerase chain reaction Methods 0.000 description 14
- 108700028369 Alleles Proteins 0.000 description 13
- 206010004446 Benign prostatic hyperplasia Diseases 0.000 description 13
- 208000004403 Prostatic Hyperplasia Diseases 0.000 description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 13
- 238000012217 deletion Methods 0.000 description 13
- 230000037430 deletion Effects 0.000 description 13
- 230000001105 regulatory effect Effects 0.000 description 13
- 241000894007 species Species 0.000 description 13
- 238000001727 in vivo Methods 0.000 description 12
- 108091008146 restriction endonucleases Proteins 0.000 description 12
- 238000010240 RT-PCR analysis Methods 0.000 description 11
- 210000004408 hybridoma Anatomy 0.000 description 11
- 238000003780 insertion Methods 0.000 description 11
- 230000037431 insertion Effects 0.000 description 11
- 102100041003 Glutamate carboxypeptidase 2 Human genes 0.000 description 10
- 101000892862 Homo sapiens Glutamate carboxypeptidase 2 Proteins 0.000 description 10
- 108091034117 Oligonucleotide Proteins 0.000 description 10
- 230000000692 anti-sense effect Effects 0.000 description 10
- 238000002347 injection Methods 0.000 description 10
- 239000007924 injection Substances 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- 239000013612 plasmid Substances 0.000 description 10
- 108020004705 Codon Proteins 0.000 description 9
- 239000000427 antigen Substances 0.000 description 9
- 108091007433 antigens Proteins 0.000 description 9
- 102000036639 antigens Human genes 0.000 description 9
- 229940079593 drug Drugs 0.000 description 9
- 239000000499 gel Substances 0.000 description 9
- 210000004602 germ cell Anatomy 0.000 description 9
- 238000002372 labelling Methods 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 8
- 102000053602 DNA Human genes 0.000 description 8
- 241000699666 Mus <mouse, genus> Species 0.000 description 8
- 239000000020 Nitrocellulose Substances 0.000 description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 8
- 230000003321 amplification Effects 0.000 description 8
- 210000000349 chromosome Anatomy 0.000 description 8
- 230000001939 inductive effect Effects 0.000 description 8
- 229920001220 nitrocellulos Polymers 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- 239000000725 suspension Substances 0.000 description 8
- 230000014616 translation Effects 0.000 description 8
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 7
- 239000011543 agarose gel Substances 0.000 description 7
- 239000000556 agonist Substances 0.000 description 7
- 239000005557 antagonist Substances 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 238000010367 cloning Methods 0.000 description 7
- 230000004069 differentiation Effects 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 238000001415 gene therapy Methods 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 230000003211 malignant effect Effects 0.000 description 7
- 238000007911 parenteral administration Methods 0.000 description 7
- 102000040430 polynucleotide Human genes 0.000 description 7
- 108091033319 polynucleotide Proteins 0.000 description 7
- 239000002157 polynucleotide Substances 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 7
- 206010027476 Metastases Diseases 0.000 description 6
- 108020004511 Recombinant DNA Proteins 0.000 description 6
- 108700009124 Transcription Initiation Site Proteins 0.000 description 6
- 238000007792 addition Methods 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- 230000002163 immunogen Effects 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 230000008488 polyadenylation Effects 0.000 description 6
- 208000023958 prostate neoplasm Diseases 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 238000000636 Northern blotting Methods 0.000 description 5
- 108700026244 Open Reading Frames Proteins 0.000 description 5
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
- 238000002105 Southern blotting Methods 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 239000004480 active ingredient Substances 0.000 description 5
- 238000000246 agarose gel electrophoresis Methods 0.000 description 5
- 230000004071 biological effect Effects 0.000 description 5
- 150000001720 carbohydrates Chemical class 0.000 description 5
- 235000014633 carbohydrates Nutrition 0.000 description 5
- 238000003018 immunoassay Methods 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 238000007726 management method Methods 0.000 description 5
- 238000010369 molecular cloning Methods 0.000 description 5
- 239000008194 pharmaceutical composition Substances 0.000 description 5
- 239000000546 pharmaceutical excipient Substances 0.000 description 5
- 238000002741 site-directed mutagenesis Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000005030 transcription termination Effects 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 239000000439 tumor marker Substances 0.000 description 5
- 239000003981 vehicle Substances 0.000 description 5
- 239000013603 viral vector Substances 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 241000187747 Streptomyces Species 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 230000002159 abnormal effect Effects 0.000 description 4
- 230000005856 abnormality Effects 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 229940024606 amino acid Drugs 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 230000000890 antigenic effect Effects 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 239000000969 carrier Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 231100000433 cytotoxic Toxicity 0.000 description 4
- 230000001472 cytotoxic effect Effects 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 238000002405 diagnostic procedure Methods 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 230000003053 immunization Effects 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000009401 metastasis Effects 0.000 description 4
- 238000000520 microinjection Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000002018 overexpression Effects 0.000 description 4
- 239000013600 plasmid vector Substances 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 4
- 208000017497 prostate disease Diseases 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 210000001082 somatic cell Anatomy 0.000 description 4
- 230000002103 transcriptional effect Effects 0.000 description 4
- 230000003612 virological effect Effects 0.000 description 4
- 239000003298 DNA probe Substances 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 206010061309 Neoplasm progression Diseases 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 241000589516 Pseudomonas Species 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 3
- 238000002820 assay format Methods 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 210000002459 blastocyst Anatomy 0.000 description 3
- 210000001109 blastomere Anatomy 0.000 description 3
- 239000013611 chromosomal DNA Substances 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 239000003937 drug carrier Substances 0.000 description 3
- 210000001671 embryonic stem cell Anatomy 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 239000003623 enhancer Substances 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 108020001507 fusion proteins Proteins 0.000 description 3
- 102000037865 fusion proteins Human genes 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 210000004907 gland Anatomy 0.000 description 3
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 3
- -1 hnRNA Proteins 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 229940124452 immunizing agent Drugs 0.000 description 3
- 230000016784 immunoglobulin production Effects 0.000 description 3
- 238000010324 immunological assay Methods 0.000 description 3
- 238000001990 intravenous administration Methods 0.000 description 3
- 238000011005 laboratory method Methods 0.000 description 3
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 3
- 230000007170 pathology Effects 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 230000004481 post-translational protein modification Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000001177 retroviral effect Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 230000000392 somatic effect Effects 0.000 description 3
- 238000007920 subcutaneous administration Methods 0.000 description 3
- 229940124597 therapeutic agent Drugs 0.000 description 3
- 230000005026 transcription initiation Effects 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 230000005751 tumor progression Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 2
- 108010051457 Acid Phosphatase Proteins 0.000 description 2
- 102000013563 Acid Phosphatase Human genes 0.000 description 2
- 229920000178 Acrylic resin Polymers 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- 201000009030 Carcinoma Diseases 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 2
- 230000006820 DNA synthesis Effects 0.000 description 2
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 2
- LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 2
- 241000701959 Escherichia virus Lambda Species 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 238000010222 PCR analysis Methods 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 230000006819 RNA synthesis Effects 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 229930182558 Sterol Natural products 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000002788 anti-peptide Effects 0.000 description 2
- 239000000074 antisense oligonucleotide Substances 0.000 description 2
- 238000012230 antisense oligonucleotides Methods 0.000 description 2
- 239000003125 aqueous solvent Substances 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 description 2
- 235000011010 calcium phosphates Nutrition 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
- 239000005547 deoxyribonucleotide Substances 0.000 description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000008121 dextrose Substances 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 238000013399 early diagnosis Methods 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 2
- 229940093471 ethyl oleate Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 231100000221 frame shift mutation induction Toxicity 0.000 description 2
- 230000037433 frameshift Effects 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 2
- 230000002414 glycolytic effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000003317 immunochromatography Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 238000007912 intraperitoneal administration Methods 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000008176 lyophilized powder Substances 0.000 description 2
- 230000036210 malignancy Effects 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 239000000346 nonvolatile oil Substances 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N phenylalanine group Chemical group N[C@@H](CC1=CC=CC=C1)C(=O)O COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 230000001323 posttranslational effect Effects 0.000 description 2
- 238000003793 prenatal diagnosis Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 239000000651 prodrug Substances 0.000 description 2
- 229940002612 prodrug Drugs 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 201000005825 prostate adenocarcinoma Diseases 0.000 description 2
- 238000000164 protein isolation Methods 0.000 description 2
- 238000001243 protein synthesis Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000007423 screening assay Methods 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000001542 size-exclusion chromatography Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 150000003432 sterols Chemical class 0.000 description 2
- 235000003702 sterols Nutrition 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 208000001608 teratocarcinoma Diseases 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 238000011426 transformation method Methods 0.000 description 2
- 230000010474 transient expression Effects 0.000 description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 241001515965 unidentified phage Species 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical group NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 1
- STQGQHZAVUOBTE-UHFFFAOYSA-N 7-Cyan-hept-2t-en-4,6-diinsaeure Natural products C1=2C(O)=C3C(=O)C=4C(OC)=CC=CC=4C(=O)C3=C(O)C=2CC(O)(C(C)=O)CC1OC1CC(N)C(O)C(C)O1 STQGQHZAVUOBTE-UHFFFAOYSA-N 0.000 description 1
- 241000203809 Actinomycetales Species 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 108020004491 Antisense DNA Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- 241000701822 Bovine papillomavirus Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000701489 Cauliflower mosaic virus Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 235000021538 Chard Nutrition 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 108010076804 DNA Restriction Enzymes Proteins 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- 108010053187 Diphtheria Toxin Proteins 0.000 description 1
- 102000016607 Diphtheria Toxin Human genes 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 108700010895 Drosophila ADH Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010001515 Galectin 4 Proteins 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Chemical group OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000282575 Gorilla Species 0.000 description 1
- 102000012428 Hematopoietic Cell Growth Factors Human genes 0.000 description 1
- 108010022580 Hematopoietic Cell Growth Factors Proteins 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 108700002232 Immediate-Early Genes Proteins 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
- 208000001019 Inborn Errors Metabolism Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 108060005987 Kallikrein Proteins 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical group OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 101710204480 Lysosomal acid phosphatase Proteins 0.000 description 1
- 102100035699 Lysosomal acid phosphatase Human genes 0.000 description 1
- 241000282560 Macaca mulatta Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 101000969137 Mus musculus Metallothionein-1 Proteins 0.000 description 1
- 102000003505 Myosin Human genes 0.000 description 1
- 108060008487 Myosin Proteins 0.000 description 1
- BKAYIFDRRZZKNF-VIFPVBQESA-N N-acetylcarnosine Chemical compound CC(=O)NCCC(=O)N[C@H](C(O)=O)CC1=CN=CN1 BKAYIFDRRZZKNF-VIFPVBQESA-N 0.000 description 1
- 208000003788 Neoplasm Micrometastasis Diseases 0.000 description 1
- 206010029260 Neuroblastoma Diseases 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 101800001442 Peptide pr Proteins 0.000 description 1
- 231100000742 Plant toxin Toxicity 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 102100035703 Prostatic acid phosphatase Human genes 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 108050006002 RNA polymerase sigma factor FliA Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 1
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000012505 Superdex™ Substances 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000007801 affinity label Substances 0.000 description 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000002669 amniocentesis Methods 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 239000003816 antisense DNA Substances 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 239000013602 bacteriophage vector Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 210000004952 blastocoel Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- BPKIGYQJPYCAOW-FFJTTWKXSA-I calcium;potassium;disodium;(2s)-2-hydroxypropanoate;dichloride;dihydroxide;hydrate Chemical compound O.[OH-].[OH-].[Na+].[Na+].[Cl-].[Cl-].[K+].[Ca+2].C[C@H](O)C([O-])=O BPKIGYQJPYCAOW-FFJTTWKXSA-I 0.000 description 1
- 230000005773 cancer-related death Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 108700010039 chimeric receptor Proteins 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- 229940107161 cholesterol Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000002967 competitive immunoassay Methods 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005094 computer simulation Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 230000000445 cytocidal effect Effects 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 229960000975 daunorubicin Drugs 0.000 description 1
- STQGQHZAVUOBTE-VGBVRHCVSA-N daunorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(C)=O)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 STQGQHZAVUOBTE-VGBVRHCVSA-N 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003405 delayed action preparation Substances 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000011496 digital image analysis Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 229960004679 doxorubicin Drugs 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000001839 endoscopy Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000010685 fatty oil Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Chemical group 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000012145 high-salt buffer Substances 0.000 description 1
- 210000003917 human chromosome Anatomy 0.000 description 1
- 210000004754 hybrid cell Anatomy 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000002390 hyperplastic effect Effects 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 208000016245 inborn errors of metabolism Diseases 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000015978 inherited metabolic disease Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000031864 metaphase Effects 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 238000012314 multivariate regression analysis Methods 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000031689 negative regulation of reverse transcription Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000012457 nonaqueous media Substances 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 150000002895 organic esters Chemical class 0.000 description 1
- 238000004223 overdiagnosis Methods 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000003169 placental effect Effects 0.000 description 1
- 239000003123 plant toxin Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000030103 pronuclear fusion Effects 0.000 description 1
- 210000000064 prostate epithelial cell Anatomy 0.000 description 1
- 108010043671 prostatic acid phosphatase Proteins 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 101150079601 recA gene Proteins 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 150000005691 triesters Chemical class 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000003932 urinary bladder Anatomy 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 210000004340 zona pellucida Anatomy 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/08—Drugs for disorders of the urinary system of the prostate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/112—Disease subtyping, staging or classification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/136—Screening for pharmacological compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Abstract
【解決手段】PCA3タンパク質をコードする核酸分子;精製されたPCA3タンパク質とポリペプチド;組換え核酸分子を含む細胞;PCA3タンパク質及びポリペプチドに特異的な結合親和性を有する抗体;PCA3タンパク質をコードする核酸を検出可能な核酸プローブ;サンプル材料中のPCA3タンパク質又はポリペプチドをコードする核酸を検出する方法;核酸プローブ又は抗体を包含するキット。その塩基配列、タンパク質、又は抗体を用いて、前立腺癌に罹患した哺乳類動物の診断、病態評定又は予後の判断を行なうためのバイオアッセイ;前立腺癌の治療方法;及び生体における前立腺癌の予防方法。
【選択図】なし
Description
本明細書においては多くの文献に参照するが、この参照によって、これらの文献の内容を本明細書に組み入れるものである。
本発明においては、PCA3又はその断片をコードする単離された核酸分子が提供される。
本発明においては、また、PCA3又はそのエピトープ含有領域であるところの精製されたポリペプチドが提供される。
本発明においては、また、PCA3タンパク質又はポリペプチドをコードする核酸が検体中に存在することを特異的に検出するための核酸が提供される。
本発明においては、また、PCA3をコードする核酸の存在を検体から検出するためのキットが提供される。
本発明においては、また、5’から3’への方向に、宿主細胞において転写を開始させるのに有効なプロモーター、及び上記の単離された核酸分子を包含する組換え核酸分子が提供される。
本発明においては、また、アンチセンスPCA3核酸分子が提供される。
本発明においては、また、上記の組換え核酸分子を含有する細胞が提供される。
本発明においては、また、PCA3又はそのエピトープ含有領域への特異的結合親和性を有する抗体が提供される。
本発明においては、また、検体中のPCA3を検出する方法が提供される。
本発明においては、また、検体中のPCA3の量を測定する方法が提供される。
本発明においては、また、上記の抗体を含有する第1容器手段と、上記の抗体と結合しうる物質と標識物質とからなる検出用結合物質の入った第2容器手段を包含する診断キットが提供される。
本発明においては、また、上記のモノクロナール抗体を産生するハイブリドーマが提供される。
本発明の更なる諸目的及び諸利益は、以下の説明から明らかとなる。
本明細書では、組換えDNA(rDNA)技術で用いられる用語が数多く使用されている。このような用語の意味のおよぶ範囲を含めて、明細書と請求の範囲の理解を明確にし、且つ、一貫させるために、以下のように定義する。
本発明の具体例の一部を示す添付の図面に参照しながら行う、本発明の好ましい態様の説明より、本発明のその他の目的、利点及び特徴は明らかになる。以下の説明は本発明を限定するものではない。
本発明をより明確に記載する目的で、本発明の詳細な説明を以下の項目に分けて記載した。これらは本発明を限定するものではない。
I.PCA3ポリペプチドをコードする単離された核酸分子
II.精製されたPCA3ポリペプチド
III.PCA3核酸を特異的に検出するための核酸プローブ
IV.サンプル材料中のPCA3核酸を検出するための方法
V.サンプル材料中のPCA3核酸を検出するためのキット
VI.PCA3核酸分子を包含するDNA構造体及びこのような構造体を含む細胞
VII.PCA3ポリペプチドに対して結合親和性を有する抗体及びこの抗体を産生するハイブリドーマ
VIII.サンプル材料中のPCA3ポリペプチド又は抗体を検出するための方法
IX.PCA3ポリペプチド又は抗体を包含する診断用キット
X.診断目的のスクリーニング
XI.治療目的の処置
XII.ヒト以外のトランスジェニックPCA3動物
本発明の一つの態様は、単離された(精製された)PCA3核酸分子に関する。PCA3核酸分子は、次の群より選ばれる塩基配列と少なくとも90%(更に好ましくは、少なくとも95%、96%、97%、98%、99%又は100%)の相同性を有する塩基配列を包含することが好ましい。
(b)Centraal voor Schimmelcultures に受託番号CBS 682.97として寄託されているポリヌクレオチドクローンがコードしている全長アミノ酸配列を包含するPCA3ポリペプチドをコードする塩基配列;
(c)Centraal voor Schimmelcultures に受託番号CBS 100512として寄託されているポリヌクレオチドクローンがコードしている全長アミノ酸配列を包含するPCA3ポリペプチドをコードする塩基配列;及び
(d)上記(a)、(b)及び(c)のいずれかの塩基配列と相補的な塩基配列。
本発明の一態様においては、PCA3に対応するアミノ酸配列を有するポリペプチドをコードする単離された核酸分子が提供される。特に、このような核酸分子は、PCA3RNA又はDNAを含む生物試料から単離することができる。
本発明の単離された核酸分子には、化学的に合成された核酸分子も含まれる。例えば、PCA3遺伝子の発現産物をコードする塩基配列を有する核酸分子を設計し、必要であれば、それを適当な、更に小さい断片にすることもできる。そして、核酸分子又はそれぞれの断片に対応するオリゴマーを合成することができる。このような合成オリゴヌクレオチドは、たとえば、Matteucciらのトリエステル法[J. Am. Chem. Soc. Vol.103, pp.3185-3191 (1981)]や自動DNA合成機を用いて製造することができる。
本発明の他の1つの態様は、PCA3に対応するアミノ酸配列を有する精製された(好ましくは実質的に純粋な)ポリペプチド、又はその機能的誘導体に関する。好ましい態様である本発明のポリペプチドの有するアミノ酸配列としては、配列番号2又は7のアミノ酸配列、或いはその突然変異体又は種間変異体のアミノ酸配列、或いは少なくとも80%の相同性又は少なくとも90%の類似性(好ましくは少なくとも90%、95%、96%、97%、98%又は99%の相同性、或いはそのポリペプチドと少なくとも95%、96%、97%、98%又は99%の類似性)を有するアミノ酸配列、或いはそのポリペプチドの中の少なくとも6個(好ましくは少なくとも10、15、20、25又は50個の隣接するアミノ酸残基)からなるアミノ酸配列が挙げられる。
更に他の1つの態様においては、本発明は、ストリンジェントな条件において、サンプル材料中のPCA3核酸の存在を特異的に検出するための、PCA3核酸と結合する上記核酸分子又はその断片を包含する核酸プローブに関する。
(c) Centraal voor Schemmelculturesに受託番号 CBS 100521として寄託されているポリヌクレオチドクローンがコードしている全長アミノ酸配列を包含するPCA3ポリペプチドをコードする塩基配列;
(e)配列番号1の1番〜98番、99番〜263番、264番〜446番、447番〜985番又は986番〜2037番の配列を包含するPCA3遺伝子のエクソンをコードする塩基配列;
(h)既に上記した塩基配列。
更に他の1つの態様においては、本発明は、サンプル材料中のPCA3核酸を検出する方法であって、a)ハイブリダイゼーションの起こりうる特定のハイブリダイゼーション条件下においてサンプル材料と上記の核酸プローブとを接触せしめ、そしてb)核酸分子に結合したプローブの存在を検出する、ことを包含する方法に関する。当業者であれば、上記したような公知の技術に従い核酸プローブを適宜選択することができる。 用いるサンプル材料の例としては、ヒト組織由来のRNA又はDNAが挙げられるが、これらに限定されるものではない。
更に他の1つの態様においては、本発明は、上記の核酸プローブの入った少なくとも1つの容器手段を包含することを特徴とする、サンプル材料中のPCA3核酸を検出するためのキットに関する。好ましい態様においては、上記のキットは更に洗浄用試薬、及び結合した核酸プローブの検出を可能にする試薬からなる群より選ばれる少なくとも1つを包含する他の容器を包含する。 検出用試薬の例としては、放射線標識プローブ、酵素標識プローブ(西洋わさびペルオキシダーゼ、 アルカリフォスファターゼ等)、アフィニティー標識プローブ(ビオチン、アビジン、又はストレプトアビジン等)が挙げられるが、これ等に限定されない。
本発明の他の態様によれば、宿主細胞において5’から3’への転写を開始するのに有効なプロモーター及び上述した核酸分子を包含する組換えDNA分子が提供される。本発明の更に他の態様によれば、ベクター及び上述した核酸分子を包含する組換えDNA分子が提供される。
更に他の1つの態様においては、本発明は上記したようなPCA3ポリペプチド又はそのPCA3ポリペプチド結合断片に対して特異的な結合親和性を有する抗体に関する。もし、抗体がPCA3ポリペプチド以外のポリペプチドに結合しないのであれば、その抗体はPCA3ポリペプチドと特異的に結合する。PCA3に選択的に結合する抗体を選択して、例えば、PCA3を含有する組織における様々なPCA3発現の分析等の方法に用いることができる。しかし、上記の抗体の利用分野はこの方法に限定されない。
更に他の1つの態様においては、本発明はサンプル材料中のPCA3ポリペプチドを検出するための方法に関する。この方法は、a)免疫複合体が形成され得る条件下において、サンプル材料と上記の抗体(タンパク質)とを接触せしめ、そして、b)PCA3ポリペプチドに結合した該抗体の存在を検出することを包含する。詳細には、サンプル材料を少なくとも1種の本発明の抗体と共にインキュベートし、抗体がサンプル材料に結合したかどうかを分析することによってサンプル材料中のPCA3ポリペプチドを検出することができる。サンプル材料中のPCA3のレベルが通常のPCA3のレベルと異なることは、特定の疾患(例えば、前立腺癌)であることを示す。
本発明の更に他の1つの態様によると、上記の検出方法を実施するために必要な試薬の全てを備えたキットが提供される。
以下の議論は特にヒト患者について述べるが、その教示はPCA3を発現するいかなる動物にも適用可能である。
A. 治療用核酸
治療剤としての治療用核酸の治療効果は特に限定されるものではないが、例えば、標的細胞に対して以下の治療効果のうちの少なくとも1種の効果を有することができる: DNA配列の転写に対する阻害;RNA配列の翻訳に対する阻害;RNAまたはDNA配列の逆転写に対する阻害;タンパク質の翻訳後修飾に対する阻害;DNA配列の転写の誘導;RNA配列の翻訳の誘導;RNAまたはDNA配列の逆転写の誘導;タンパク質の翻訳後修飾の誘導;治療用核酸のRNAへの転写;治療用核酸のタンパク質または酵素への翻訳;及び、治療用核酸を標的細胞の染色体へ組み込むことによる、標的細胞での構成的または一時的な発現。
PCA3のアンタゴニストとアゴニストがPCA3の活性をそれぞれ阻害及び亢進する能力は、PCA3を含む細胞を用いて評価することができる。アンタゴニストやアゴニストとして挙動する作用物質(agent)の存在下でのPCA3タンパク質の機能を測定するための、細胞内のPCA3活性のアッセイを行なうことができ、こうして、PCA3の活性を阻害する作用物質と亢進する作用物質を同定することができる。
(a)PCA3を発現する細胞を、試験に供される作用物質と共にインキュベートし;そして
(b)PCA3のATPへの結合に及ぼす作用物質の効果を測定して、細胞におけるPCA3タンパク質の活性を評価する。
本発明はまた、in vivoにおいてPCA3の生物活性を阻害又は中和し、且つPCA3に特異的な、上記したPCA3抗体(好ましくはマウス抗PCA3抗体及びマウス−ヒト抗PCA3キメラ抗体、並びにその断片及び領域)を提供する。これらの抗体は、PCA3の異常な発現に伴う病態(pathologies and conditions)を示す患者の治療を目的として使用することができる。本発明の抗体並びにその断片、領域及び誘導体は、in vivoにおいて上記PCA3の生物活性を阻害及び/または中和することができる、PCA3のエピトープを認識する領域を少なくとも1つ含むことが好ましい。
ヒト以外のトランスジェニックPCA3動物の作成方法
本発明における、ヒト以外の動物は、形質転換により内因性遺伝子が分断または改変されている動物(ノックアウト動物)、及び/またはヒトPCA3の発現に繋がる1種以上の導入遺伝子(transgenes)がそのゲノムに導入されている動物を包含する。アンチセンスPCA3核酸を導入されている動物もまた好ましい。
前立腺癌の新しいマーカーを同定するために、differential display analysis (Liang et al., Science 257: 967-971, 1992)を用いて、正常な前立腺と比較して、前立腺癌で過剰発現している遺伝子を同定した。具体的には、同一患者の正常な前立腺組織、良性前立腺肥大(BPH)組織及び悪性前立腺組織のそれぞれから全RNAを抽出して解析を行った。4つのアンカープライマーと5つの任意のプライマーによる20種類の異なるプライマーセットを用いて、両者で見かけ上異なった発現をする11種のmRNA(即ち、調べた全ての癌組織においては一貫して過剰発現しているが、正常組織或いはBPH組織においては発現していないmRNA)を同定した。これらのmRNAの相補鎖DNA(cDNA)断片をノーザンブロット解析にプローブとして用いて、differential display法で調べた前立腺腫瘍における一貫した過剰発現を確認した。こうしたプローブの1つであるDD3(486bpのcDNAプローブ)によって、調べたヒト前立腺腫瘍50例中47例において高度に過剰発現している2.3kb並びに4.0kbの2つの主要な転写物を検出した。一方、同一患者の正常組織またはBPH組織においては、これらの転写物の発現は全くみられなかった(又は非常に低レベルであった)。
PCA3に特異的なプライマーを開発する際に、いくつかの正常ヒト組織から抽出したRNAを用いてRT−PCR解析を行った。40サイクルのPCRで、正常前立腺組織及びBPH組織におけるPCA3関連産物が増幅された。PCA3の発現は、非常に前立腺特異的である。何故なら、以下の正常ヒト組織においては、同様の条件下でPCA3産物を全く増幅することができなかったからである。調べた組織は、動脈、脳、前胸部、膀胱、大腸、十二指腸、心臓、肝臓、肺、卵巣、膵臓、胎盤、精嚢、骨格筋、皮膚、脊髄、脾臓並びに精巣である。ヒト前立腺癌由来の細胞株であるALVA−31、DU145、JCA−1、PPC−1、PC3並びにTSU−Pr1においても、PCA3関連PCR産物は全く検出できなかった。ヒト前立腺アデノカルシノーマ細胞株LNCaPでは、40サイクルのPCRで産物を得ることができる(一方、同じ条件下で、前立腺腫瘍では20サイクル以内で産物を得ることができる)。前立腺に特異的なPCA3の発現の評価に用いた技術は、前立腺癌の診断試験に適応することができる。その上、前立腺を原発巣とする転移巣の同定に適応することもできる。
PCA3の転写開始部位を決定するために、プライマー・エクステンション法、ヌクレアーゼS1マッピング、5’RACE(急速cDNA末端増幅法)を行った。その結果、主要な転写開始部位が4塩基の範囲内に局在することが見出された(図4参照)。
本発明の好ましい態様について説明したが、本明細書に添付のクレームによって定義された本発明の本質及びその性質から逸脱することなく本発明の説明を改変することは可能である。
Claims (26)
- 次の(a)〜(c)からなる群より選ばれる単離された核酸分子。
(a)正常ヒト組織と比べて、前立腺癌組織において過剰発現していることを特徴とする、配列番号1、3、4又は6からなる塩基配列;
(b)上記(a)の塩基配列に完全に相補的な塩基配列;及び
(c)上記(a)又は(b)の塩基配列の中の少なくとも10個の連続した塩基にストリンジェントな条件下でハイブリダイズする少なくとも15塩基からなる塩基配列であって、PCA3核酸を特異的に増幅するか又は検出することのできる塩基配列。 - 正常ヒト組織と比べて、前立腺癌組織において過剰発現している単離された核酸分子であって、次の(a)と(b)からなる群より選ばれる単離された核酸分子。
(a)配列番号1、3、4又は6の塩基配列;及び
(b)配列番号1、3、4又は6の塩基配列に完全に相補的な塩基配列の中の少なくとも10個の連続した塩基にストリンジェントな条件下でハイブリダイズする少なくとも15塩基からなる塩基配列であって、PCA3核酸を特異的に増幅するか又は検出することのできる塩基配列。 - 請求項1で定義した核酸分子の部分配列からなり、該部分配列が次の(a)と(b)からなる群より選ばれることを特徴とする単離された核酸分子。
(a)以下のPCA3遺伝子のエクソン又はそれらの組み合わせから選ばれる連続した15〜50塩基からなる塩基配列:エクソン1(配列番号1の1番〜98番の配列又は配列番号6の1番〜120番の配列)、エクソン2(配列番号1の99番〜263番の配列又は配列番号6の121番〜285番の配列)、エクソン3(配列番号1の264番〜446番の配列又は配列番号6の286番〜468番の配列)、エクソン4a(配列番号1の447番〜985番の配列又は配列番号6の469番〜1007番の配列)、エクソン4b(配列番号1の986番〜2037番の配列又は配列番号6の1008番〜2066番の配列)、エクソン4c(配列番号6の2067番〜2622番の配列)とエクソン4d(配列番号6の2623番〜3582番の配列);及び
(b)上記(a)の塩基配列に相補的な塩基配列。 - 該ストリンジェントな条件が、5×SSC、5×デンハルト溶液、1% SDS及び100μg/mlの変性サケ精巣DNAを含む溶液中で68℃のハイブリダイゼーション条件であることを特徴とする、請求項1又は2に記載の単離された核酸分子。
- 該正常ヒト組織が、動脈、脳、前胸部、十二指腸、心臓、肝臓、卵巣、胎盤、精嚢、骨格筋、皮膚、脊髄、脾臓、精巣及び前立腺からなる群より選ばれるものであることを特徴とする、請求項1〜4のいずれかに記載の単離された核酸分子。
- サンプル材料中のPCA3核酸を検出する方法にして、
a)ハイブリダイゼーションの起こりうる条件下において該サンプル材料と請求項1〜5のいずれかの核酸分子とを接触せしめ、
b)PCA3核酸に結合した該核酸分子の存在を検出する、
ことを包含する方法。 - 正常ヒト組織と比べて、前立腺癌組織において過剰発現しているPCA3核酸を検出するためのキットであって、請求項1〜5のいずれかの核酸分子の入った少なくとも1つの容器手段を包含することを特徴とするキット。
- 次の(a)と(b)からなる群より選ばれる単離された核酸分子。
(a)正常ヒト組織と比べて、前立腺癌組織において過剰発現していることを特徴とする、配列番号6の401番〜553番塩基からなる塩基配列;及び
(b)上記(a)の塩基配列に完全に相補的な塩基配列。 - 配列番号1の塩基配列からなることを特徴とする、請求項1又は2に記載の単離された核酸分子。
- 配列番号3の塩基配列からなることを特徴とする、請求項1又は2に記載の単離された核酸分子。
- 配列番号4の塩基配列からなることを特徴とする、請求項1又は2に記載の単離された核酸分子。
- 配列番号6の塩基配列からなることを特徴とする、請求項1又は2に記載の単離された核酸分子。
- 該単離された核酸分子が、ALVA−31、JCA−1、PPC−1、DU145、PC3及びTSU−Pr1からなる群より選ばれるヒト前立腺癌由来の細胞株と比較して、前立腺癌組織において過剰発現していることを特徴とする、請求項1〜5と8〜12のいずれかに記載の単離された核酸分子。
- ベクターと、請求項1〜5と8〜13のいずれかの核酸分子とを包含することを特徴とする組換え核酸分子。
- 請求項14の組換え核酸分子を含む単離された細胞。
- 生物試料における前立腺癌細胞の存在又は癌化する素因を有する前立腺細胞の存在を検出するための方法であって、
請求項1〜5と8〜13のいずれかの単離された核酸分子を用いて生物試料中のPCA3核酸の量を測定し、正常前立腺組織と比べて、該生物試料において該PCA3核酸が過剰発現していることをもって、該生物試料中に前立腺癌細胞又は癌化する素因を有する前立腺細胞が存在すると判定する
ことを包含する検出方法。 - 該PCA3核酸がPCA3 mRNAであることを特徴とする、請求項16に記載の検出方法。
- 5’から3’への方向に、宿主細胞において転写を開始させるのに有効なプロモーター、及び請求項1〜5と8〜13のいずれかの核酸分子を包含することを特徴とする組換え核酸分子。
- 請求項17の組換え核酸分子を含むヒト以外の生物。
- 該塩基配列(b)が、塩基配列(a)に完全に相補的であることを特徴する、請求項3に記載の単離された核酸分子。
- 少なくとも15塩基からなる塩基配列が、15〜50塩基からなる塩基配列であることを特徴とする、請求項1又は2に記載の単離された核酸分子。
- 患者から得たサンプル材料中のPCA3核酸を定量することによって、該患者の有する前立腺癌又は前立腺癌の素因を診断するための診断用キットであって、請求項3又は4の核酸分子の入った少なくとも1つの容器手段を包含することを特徴とするキット。
- 該PCA3核酸がPCA3 mRNAであることを特徴とする、請求項22に記載のキット。
- 該サンプル材料が、器官、組織、細胞、細胞抽出物及び体液からなる群より選ばれることを特徴とする、請求項6、16又は17に記載の方法、あるいは請求項7、22又は23に記載のキット。
- 該体液が、血液、血清、血漿及び尿からなる群より選ばれることを特徴とする、請求項24に記載の方法又はキット。
- 該体液が尿であることを特徴とする、請求項25に記載の方法又はキット。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4183697P | 1997-04-10 | 1997-04-10 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54219498A Division JP3981416B2 (ja) | 1997-04-10 | 1998-04-09 | Pca3タンパク質、pca3遺伝子、及びこれらの用途 |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2007275069A true JP2007275069A (ja) | 2007-10-25 |
Family
ID=21918594
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54219498A Expired - Lifetime JP3981416B2 (ja) | 1997-04-10 | 1998-04-09 | Pca3タンパク質、pca3遺伝子、及びこれらの用途 |
JP2007125170A Pending JP2007275069A (ja) | 1997-04-10 | 2007-05-10 | Pca3タンパク質、pca3遺伝子、及びこれらの用途 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54219498A Expired - Lifetime JP3981416B2 (ja) | 1997-04-10 | 1998-04-09 | Pca3タンパク質、pca3遺伝子、及びこれらの用途 |
Country Status (9)
Country | Link |
---|---|
US (5) | US7008765B1 (ja) |
EP (2) | EP1007650B1 (ja) |
JP (2) | JP3981416B2 (ja) |
AT (1) | ATE426018T1 (ja) |
AU (1) | AU7019498A (ja) |
CA (1) | CA2286304C (ja) |
DE (1) | DE69840669D1 (ja) |
ES (2) | ES2402947T3 (ja) |
WO (1) | WO1998045420A1 (ja) |
Families Citing this family (53)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6329505B1 (en) | 1997-02-25 | 2001-12-11 | Corixa Corporation | Compositions and methods for therapy and diagnosis of prostate cancer |
US7270980B2 (en) * | 1997-02-25 | 2007-09-18 | Corixa Corporation | Compounds for immunodiagnosis of prostate cancer and methods for their use |
ATE426018T1 (de) | 1997-04-10 | 2009-04-15 | Stichting Katholieke Univ | Pca3, pca3-gene und verfahren zu ihrer verwendung |
DE19836559A1 (de) * | 1998-08-12 | 2000-03-23 | Antigen Gmbh | Gefäß zur Entnahme von Blut |
US20060204989A1 (en) * | 1998-09-22 | 2006-09-14 | Kopreski Michael S | Comparative analysis of extracellular RNA species |
EP1206534A1 (en) * | 1999-08-19 | 2002-05-22 | Genset | Prostate cancer-related gene 3 (pg3) and biallelic markers thereof |
DE60027027T2 (de) | 1999-09-29 | 2006-11-30 | Diagnocure Inc. | Pca3 mrna in gutartigen und bösartigen prostatageweben |
US6897024B2 (en) | 2001-05-31 | 2005-05-24 | Stichting Katholieke Universiteit More Particularly The University Medical Centre Nijmegen | Nucleic acid molecules comprising the promoter for PCA3, and uses thereof |
US20100159464A1 (en) * | 2001-11-05 | 2010-06-24 | Oncomedx, Inc. | Method for Detection of DNA Methyltransferase RNA in Plasma and Serum |
DE10230692A1 (de) * | 2002-07-08 | 2004-02-12 | Epigenomics Ag | Verfahren und Nukleinsäuren für die Analyse von Methylierungsmustern innerhalb des DD3-Gens |
EP1592809B1 (en) * | 2003-02-07 | 2013-04-10 | Diagnocure Inc. | Method to detect prostate cancer in a sample |
CA2432365A1 (en) | 2003-06-30 | 2004-12-30 | Jack A. Schalken | Specific method of prostate cancer detection based on pca3, and kits therefore |
AU2005245815B2 (en) | 2004-05-07 | 2011-06-09 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. | Methods of diagnosing or treating prostate cancer using the erg gene, alone or in combination with other over or under expressed genes in prostate cancer |
US11105808B2 (en) | 2004-11-12 | 2021-08-31 | Health Discovery Corporation | Methods for screening, predicting and monitoring prostate cancer |
CA2491067A1 (en) | 2004-12-24 | 2006-06-24 | Stichting Katholieke Universiteit | Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer |
US7496340B1 (en) * | 2005-06-02 | 2009-02-24 | Rf Micro Devices, Inc. | I/Q mismatch calibration of direct conversion receivers using radio frequency noise |
EP2612870A1 (en) | 2005-09-12 | 2013-07-10 | The Regents of the University of Michigan | Recurrent gene fusions in prostate cancer |
US9957569B2 (en) | 2005-09-12 | 2018-05-01 | The Regents Of The University Of Michigan | Recurrent gene fusions in prostate cancer |
WO2008058018A2 (en) | 2006-11-02 | 2008-05-15 | Mayo Foundation For Medical Education And Research | Predicting cancer outcome |
EP2079851B1 (en) * | 2006-11-08 | 2015-01-07 | The Regents Of The University Of Michigan | Method using spink1 as a prostate cancer marker |
EP2121956B1 (en) | 2006-12-21 | 2016-08-17 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
CA2692441C (en) | 2007-07-06 | 2020-01-21 | The Regents Of The University Of Michigan | Solute carrier family 45 member 3 (slc45a3) and ets family gene fusions in prostate cancer |
US9303291B2 (en) | 2007-07-06 | 2016-04-05 | The Regents Of The University Of Michigan | MIPOL1-ETV1 gene rearrangements |
EP2597464B1 (en) | 2007-08-16 | 2015-02-25 | The Regents of the University of Michigan | Metabolomic profiling of prostate cancer |
PL2187965T3 (pl) | 2007-08-17 | 2020-05-18 | Purdue Research Foundation | Koniugaty wiążący psma ligand-łącznik i sposoby ich zastosowania |
WO2009140741A1 (en) * | 2008-05-23 | 2009-11-26 | The University Of Queensland | Agents and methods for diagnosing the presence or risk of prostate cancer |
EP2291553A4 (en) | 2008-05-28 | 2011-12-14 | Genomedx Biosciences Inc | SYSTEMS AND METHODS FOR THE EXPRESSION-BASED DISTINCTION OF DIFFERENT CLINICAL ILLICIT STADIAS IN PROSTATE CANCER |
US10407731B2 (en) | 2008-05-30 | 2019-09-10 | Mayo Foundation For Medical Education And Research | Biomarker panels for predicting prostate cancer outcomes |
US8202690B2 (en) | 2008-11-28 | 2012-06-19 | Hisamitsu Pharmaceutical Co., Inc. | Cancer marker and therapeutic agent for cancer |
US20120015839A1 (en) | 2009-01-09 | 2012-01-19 | The Regents Of The University Of Michigan | Recurrent gene fusions in cancer |
US20100311815A1 (en) * | 2009-02-23 | 2010-12-09 | The Regents Of The University Of Michigan | Mir-101 cancer markers |
US9169512B2 (en) | 2009-07-01 | 2015-10-27 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
JP5800817B2 (ja) | 2009-09-17 | 2015-10-28 | ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン | 前立腺癌における再発性遺伝子融合 |
US9951324B2 (en) | 2010-02-25 | 2018-04-24 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
EP2407554A1 (en) | 2010-07-14 | 2012-01-18 | Fundacio Institut de Recerca de l'Hospital Universitari Vall d'Hebron | Methods and kits for the diagnosis of prostate cancer |
EP2407555A1 (en) | 2010-07-14 | 2012-01-18 | Fundació Institut de Recerca Hospital Universitari Vall d'Hebron, Fundació Privada | Methods and kits for the diagnosis of prostate cancer |
CN103403181B (zh) | 2010-11-19 | 2016-09-07 | 密执安大学评议会 | ncRNA及其用途 |
US8945556B2 (en) | 2010-11-19 | 2015-02-03 | The Regents Of The University Of Michigan | RAF gene fusions |
US20130267443A1 (en) | 2010-11-19 | 2013-10-10 | The Regents Of The University Of Michigan | ncRNA AND USES THEREOF |
CA2858581A1 (en) | 2011-12-13 | 2013-06-20 | Genomedx Biosciences, Inc. | Cancer diagnostics using non-coding transcripts |
AU2013302365B2 (en) | 2012-08-16 | 2019-03-21 | Mayo Foundation For Medical Education And Research | Cancer diagnostics using biomarkers |
KR20150104092A (ko) | 2012-11-15 | 2015-09-14 | 엔도사이트, 인코포레이티드 | Psma 발현 세포에 의해 야기되는 질병을 치료하기 위한 컨쥬게이트 |
FI4095130T3 (fi) | 2013-10-18 | 2024-04-25 | Novartis Ag | Prostataspesifisen membraaniantigeenin (psma) leimattuja estäjiä, niiden käyttö kuvantamisaineina ja farmaseuttisia aineita eturauhassyövän hoitoon |
JP2018504595A (ja) | 2015-01-05 | 2018-02-15 | ユニバーシティ オブ オスロUniversity of Oslo | 前立腺癌マーカー及びその利用 |
US10188759B2 (en) | 2015-01-07 | 2019-01-29 | Endocyte, Inc. | Conjugates for imaging |
US9528096B1 (en) * | 2016-06-30 | 2016-12-27 | Fornia Biosolutions, Inc. | Phytases and uses thereof |
US10351832B2 (en) | 2016-06-30 | 2019-07-16 | Fornia Biosolutions, Inc. | Phytases and uses thereof |
US9605245B1 (en) | 2016-06-30 | 2017-03-28 | Fornia BioSoultions, Inc. | Phytases and uses thereof |
US11414708B2 (en) | 2016-08-24 | 2022-08-16 | Decipher Biosciences, Inc. | Use of genomic signatures to predict responsiveness of patients with prostate cancer to post-operative radiation therapy |
WO2018132916A1 (en) | 2017-01-20 | 2018-07-26 | Genomedx Biosciences, Inc. | Molecular subtyping, prognosis, and treatment of bladder cancer |
EP3593140A4 (en) | 2017-03-09 | 2021-01-06 | Decipher Biosciences, Inc. | SUBTYPING PROSTATE CANCER TO PREDICT RESPONSE TO HORMONE THERAPY |
EP3622087A4 (en) | 2017-05-12 | 2021-06-16 | Decipher Biosciences, Inc. | GENETIC SIGNATURES FOR PREDICTING PROSTATE CANCER METASTASIS AND IDENTIFYING TUMOR VIRULENCE |
WO2024054924A1 (en) | 2022-09-08 | 2024-03-14 | Gen-Probe Incorporated | Method of detecting nucleic acid analytes using dual-specificity primers |
Family Cites Families (101)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US763243A (en) * | 1903-10-26 | 1904-06-21 | William P Bartholow | Self-heating soldering-iron. |
FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
US4690890A (en) | 1984-04-04 | 1987-09-01 | Cetus Corporation | Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay |
US4786600A (en) * | 1984-05-25 | 1988-11-22 | The Trustees Of Columbia University In The City Of New York | Autocatalytic replication of recombinant RNA |
JPS6147500A (ja) | 1984-08-15 | 1986-03-07 | Res Dev Corp Of Japan | キメラモノクロ−ナル抗体及びその製造法 |
EP0173494A3 (en) | 1984-08-27 | 1987-11-25 | The Board Of Trustees Of The Leland Stanford Junior University | Chimeric receptors by dna splicing and expression |
GB8422238D0 (en) | 1984-09-03 | 1984-10-10 | Neuberger M S | Chimeric proteins |
JPS61134325A (ja) | 1984-12-04 | 1986-06-21 | Teijin Ltd | ハイブリツド抗体遺伝子の発現方法 |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
WO1987002671A1 (en) | 1985-11-01 | 1987-05-07 | International Genetic Engineering, Inc. | Modular assembly of antibody genes, antibodies prepared thereby and use |
US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US4851331A (en) * | 1986-05-16 | 1989-07-25 | Allied Corporation | Method and kit for polynucleotide assay including primer-dependant DNA polymerase |
FR2602592B1 (fr) | 1986-08-06 | 1989-06-30 | Alain Baret | Utilisation du systeme enzymatique xanthine-oxydase en immuno-analyse, procedes de dosage correspondants et coffrets de reactifs necessaires pour la mise en oeuvre de ces procedes. |
US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
US5202231A (en) * | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
US5149625A (en) * | 1987-08-11 | 1992-09-22 | President And Fellows Of Harvard College | Multiplex analysis of DNA |
US5283174A (en) * | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
US5639604A (en) * | 1987-09-21 | 1997-06-17 | Gen-Probe Incorporated | Homogeneous protection assay |
US5585481A (en) * | 1987-09-21 | 1996-12-17 | Gen-Probe Incorporated | Linking reagents for nucleotide probes |
CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
US5002867A (en) * | 1988-04-25 | 1991-03-26 | Macevicz Stephen C | Nucleic acid sequence determination by multiple mixed oligonucleotide probes |
NL8801147A (nl) * | 1988-05-02 | 1989-12-01 | Tno | Werkwijze voor het detecteren van genetische variatie, en daarvoor geschikte kit. |
DK167254B1 (da) | 1988-07-21 | 1993-09-27 | Hartmann As Brdr | Fremgangsmaade til fremstilling af formede genstande af et fluidiseret cellulosefibermateriale |
US5183949A (en) | 1988-09-22 | 1993-02-02 | The United States Of America As Represented By The Department Of Health And Human Services | Rabbit model for diagnosing and testing vaccines or therapeutic agents against aids |
US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
US5219989A (en) * | 1988-12-13 | 1993-06-15 | Mcgill University | Bifunctional protein for the isolation of capped mRNA |
US5082670A (en) | 1988-12-15 | 1992-01-21 | The Regents Of The University Of California | Method of grafting genetically modified cells to treat defects, disease or damage or the central nervous system |
US5175383A (en) | 1989-02-17 | 1992-12-29 | President And Fellows Of Harvard College | Animal model for benign prostatic disease |
US5800992A (en) * | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
US5112736A (en) * | 1989-06-14 | 1992-05-12 | University Of Utah | Dna sequencing using fluorescence background electroblotting membrane |
US5656207A (en) * | 1989-06-24 | 1997-08-12 | Gen Probe Incorporated | Detecting or quantifying multiple analytes using labelling techniques |
US5174986A (en) | 1989-07-05 | 1992-12-29 | Genpharm International, Inc. | Method for determining oncogenic potential of a chemical compound |
CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
IE911869A1 (en) | 1990-06-01 | 1991-12-04 | Regeneron Pharma | A family of map2 protein kinases |
WO1992006693A1 (en) | 1990-10-22 | 1992-04-30 | Fox Chase Cancer Center | Dna construct for providing rna therapy |
AU662906B2 (en) | 1991-06-26 | 1995-09-21 | F. Hoffmann-La Roche Ag | Methods for detection of carcinoma metastases by nucleic acid amplification |
CA2082411A1 (en) | 1991-06-28 | 1992-12-29 | Robert D. Rosenberg | Localized oligonucleotide therapy |
WO1993003743A1 (en) | 1991-08-16 | 1993-03-04 | The General Hospital Corporation | Method of gene delivery to post-mitotic cells |
WO1993008845A1 (en) | 1991-11-08 | 1993-05-13 | Massachusetts Institute Of Technology | Localized oligonucleotide therapy |
WO1993012236A1 (en) | 1991-12-16 | 1993-06-24 | E.I. Du Pont De Nemours And Company | Constitutive expression of p450soy and ferredoxin-soy in streptomyces, and biotransformation of chemicals by recombinant organisms |
DE69233599T2 (de) | 1991-12-24 | 2006-12-14 | Isis Pharmaceuticals, Inc., Carlsbad | Unterbrochene 2'-modifizierte Oligonukleotide |
JP4137996B2 (ja) * | 1992-05-06 | 2008-08-20 | ジェン−プローブ・インコーポレイテッド | 核酸配列増幅方法,組成物及びキット |
US5674682A (en) * | 1992-10-29 | 1997-10-07 | Thomas Jefferson University | Nucleic acid primers for detecting micrometastasis of prostate cancer |
ATE342356T1 (de) * | 1992-11-05 | 2006-11-15 | Sloan Kettering Inst Cancer | Prostata-spezifisches membranantigen |
US5503980A (en) * | 1992-11-06 | 1996-04-02 | Trustees Of Boston University | Positional sequencing by hybridization |
WO1994012627A1 (en) | 1992-11-25 | 1994-06-09 | Cephalon, Inc. | Transgenic animal model for alzheimer's disease |
US5773705A (en) | 1992-12-31 | 1998-06-30 | Wisconsin Alumni Research Foundation | Ubiquitin fusion protein system for protein production in plants |
DK0690726T3 (da) | 1993-01-07 | 2002-03-11 | Univ Jefferson | Antisense-inhibibering af c-myc til modulering af proliferationen af glat muskel-celler |
US5422252A (en) * | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
US5861242A (en) * | 1993-06-25 | 1999-01-19 | Affymetrix, Inc. | Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same |
US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
WO1995003428A1 (en) * | 1993-07-20 | 1995-02-02 | University Of Massachusetts Medical Center | In vivo nucleic acid hybridization method |
US6323184B1 (en) | 1993-10-15 | 2001-11-27 | Thomas Jefferson University | Arteriovenous and venous graft treatments: methods and compositions |
US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
AU687535B2 (en) * | 1994-03-16 | 1998-02-26 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
EP0760006A1 (en) * | 1994-04-15 | 1997-03-05 | The Trustees Of Columbia University In The City Of New York | Method for molecular staging of prostate cancer |
WO1995032305A1 (en) | 1994-05-19 | 1995-11-30 | Dako A/S | Pna probes for detection of neisseria gonorrhoeae and chlamydia trachomatis |
AU3889595A (en) | 1994-10-05 | 1996-05-02 | Amgen, Inc. | Method for inhibiting smooth muscle cell proliferation and oligonucleotides for use therein |
EP0709466B1 (en) * | 1994-10-28 | 2006-09-27 | Gen-Probe Incorporated | Compositions and methods for the simultaneous detection and quantification of multiple specific nucleic acid sequences |
US5919652A (en) * | 1994-11-09 | 1999-07-06 | The Regents Of The University Of California | Nucleic acid molecules comprising the prostate specific antigen (PSA) promoter and uses thereof |
DE19513152A1 (de) | 1995-04-07 | 1996-10-10 | Bundesrep Deutschland | Verwendung eines "Immundefizienzvirus-supprimierenden Lymphokins (ISL)" zur Hemmung der Virusvermehrung, insbesondere von Retroviren |
US5731148A (en) | 1995-06-07 | 1998-03-24 | Gen-Probe Incorporated | Adduct protection assay |
US6130038A (en) | 1996-07-16 | 2000-10-10 | Gen-Probe Incorporated | Method for amplifying target nucleic acids using modified primers |
US6479263B1 (en) * | 1996-11-14 | 2002-11-12 | Baylor College Of Medicine | Method for detection of micrometastatic prostate cancer |
US6261562B1 (en) | 1997-02-25 | 2001-07-17 | Corixa Corporation | Compounds for immunotherapy of prostate cancer and methods for their use |
US6800746B2 (en) | 1997-02-25 | 2004-10-05 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of prostate cancer |
US6395278B1 (en) * | 1997-02-25 | 2002-05-28 | Corixa Corporation | Prostate specific fusion protein compositions |
CA2281952C (en) | 1997-02-25 | 2011-07-19 | Corixa Corporation | Compounds for immunotherapy of prostate cancer and methods for their use |
US6465611B1 (en) * | 1997-02-25 | 2002-10-15 | Corixa Corporation | Compounds for immunotherapy of prostate cancer and methods for their use |
ATE426018T1 (de) * | 1997-04-10 | 2009-04-15 | Stichting Katholieke Univ | Pca3, pca3-gene und verfahren zu ihrer verwendung |
US6534273B2 (en) * | 1997-05-02 | 2003-03-18 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
ES2271994T3 (es) * | 1997-05-02 | 2007-04-16 | Gen-Probe Incorporated | Hibridacion en dos pasos y captura de un polinucleotido. |
ES2355631T3 (es) * | 1997-05-30 | 2011-03-29 | Trovagene, Inc. | Procedimiento para detectar secuencias de ácidos nucleicos en la orina. |
US20020035244A1 (en) | 1997-09-09 | 2002-03-21 | Maurice Cohen | Reagents and methods useful for detecting diseases of the prostate |
TW200532020A (en) | 1998-07-14 | 2005-10-01 | Corixa Corp | Compositions and methods for therapy and diagnosis of prostate cancer |
US6037130A (en) * | 1998-07-28 | 2000-03-14 | The Public Health Institute Of The City Of New York, Inc. | Wavelength-shifting probes and primers and their use in assays and kits |
AU767587B2 (en) * | 1999-01-28 | 2003-11-20 | Gen-Probe Incorporated | Nucleic acid sequences for detecting genetic markers for cancer in a biological sample |
FI990382A0 (fi) | 1999-02-23 | 1999-02-23 | Arctic Partners Oy Ab | Uusi diagnostinen menetelmä |
US6528260B1 (en) * | 1999-03-25 | 2003-03-04 | Genset, S.A. | Biallelic markers related to genes involved in drug metabolism |
CA2368385C (en) | 1999-03-26 | 2012-05-15 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Prostate-specific gene, pcgem1, and methods of using pcgem1 to detect, treat, and prevent prostate cancer |
DE60027027T2 (de) * | 1999-09-29 | 2006-11-30 | Diagnocure Inc. | Pca3 mrna in gutartigen und bösartigen prostatageweben |
AU7859900A (en) | 1999-10-04 | 2001-05-10 | Corixa Corporation | Compositions and methods for wt1 specific immunotherapy |
WO2001025272A2 (en) | 1999-10-04 | 2001-04-12 | Corixa Corporation | Compositions and methods for therapy and diagnosis of prostate cancer |
AU1656501A (en) | 1999-11-12 | 2001-06-06 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of prostate cancer |
WO2001044507A1 (en) | 1999-12-17 | 2001-06-21 | Bio Merieux | Process for labeling a nucleic acid |
AU3447401A (en) | 2000-01-14 | 2001-07-24 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of prostate cancer |
AU2001236519A1 (en) * | 2000-01-24 | 2001-07-31 | Millennium Pharmaceuitcals, Inc. | Identification, assessment, prevention, and therapy of prostate cancer |
AU2001241541A1 (en) | 2000-02-17 | 2001-08-27 | Millennium Predictive Medicine, Inc. | Novel genes, compositions, kits, and methods for identification, assessment, prevention, and therapy of human prostate cancer |
JP2004504808A (ja) | 2000-03-27 | 2004-02-19 | コリクサ コーポレイション | 前立腺癌の治療及び診断のための組成物及び方法 |
FR2810114B1 (fr) | 2000-06-13 | 2002-08-23 | Bio Merieux | Methode, procede, test immunologique et kit de diagnostic d'un adenocarcinome de la prostate ou d'une hypertrophie benigne de la prostate |
US20030031678A1 (en) | 2000-09-19 | 2003-02-13 | Shujath Ali | Compositions and methods relating to prostate specific genes and proteins |
EP1474528A4 (en) | 2000-10-13 | 2006-06-14 | Protein Design Labs Inc | METHODS FOR DIAGNOSING PROSTATE CANCER, COMPOSITIONS AND METHODS FOR SCREENING PROSTATE CANCER MODULATORS |
US6897024B2 (en) * | 2001-05-31 | 2005-05-24 | Stichting Katholieke Universiteit More Particularly The University Medical Centre Nijmegen | Nucleic acid molecules comprising the promoter for PCA3, and uses thereof |
EP1592809B1 (en) | 2003-02-07 | 2013-04-10 | Diagnocure Inc. | Method to detect prostate cancer in a sample |
CA2432365A1 (en) * | 2003-06-30 | 2004-12-30 | Jack A. Schalken | Specific method of prostate cancer detection based on pca3, and kits therefore |
CA2491067A1 (en) * | 2004-12-24 | 2006-06-24 | Stichting Katholieke Universiteit | Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer |
IT1395574B1 (it) | 2009-09-14 | 2012-10-16 | Guala Dispensing Spa | Dispositivo di erogazione |
US20140371088A1 (en) * | 2013-06-14 | 2014-12-18 | Nanostring Technologies, Inc. | Multiplexable tag-based reporter system |
-
1998
- 1998-04-09 AT AT98916696T patent/ATE426018T1/de active
- 1998-04-09 EP EP98916696A patent/EP1007650B1/en not_active Expired - Lifetime
- 1998-04-09 ES ES09003774T patent/ES2402947T3/es not_active Expired - Lifetime
- 1998-04-09 US US09/402,713 patent/US7008765B1/en not_active Expired - Lifetime
- 1998-04-09 AU AU70194/98A patent/AU7019498A/en not_active Abandoned
- 1998-04-09 ES ES98916696T patent/ES2324503T3/es not_active Expired - Lifetime
- 1998-04-09 DE DE69840669T patent/DE69840669D1/de not_active Expired - Lifetime
- 1998-04-09 WO PCT/CA1998/000346 patent/WO1998045420A1/en active Application Filing
- 1998-04-09 EP EP09003774A patent/EP2060630B1/en not_active Expired - Lifetime
- 1998-04-09 JP JP54219498A patent/JP3981416B2/ja not_active Expired - Lifetime
- 1998-04-09 CA CA002286304A patent/CA2286304C/en not_active Expired - Lifetime
-
2005
- 2005-10-28 US US11/260,282 patent/US7632643B2/en not_active Expired - Fee Related
-
2007
- 2007-05-10 JP JP2007125170A patent/JP2007275069A/ja active Pending
-
2009
- 2009-10-30 US US12/609,483 patent/US8551699B2/en not_active Expired - Fee Related
-
2013
- 2013-09-23 US US14/033,699 patent/US9540696B2/en not_active Expired - Fee Related
-
2016
- 2016-11-30 US US15/364,962 patent/US20170073776A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US8551699B2 (en) | 2013-10-08 |
JP2001522240A (ja) | 2001-11-13 |
US7008765B1 (en) | 2006-03-07 |
EP1007650A1 (en) | 2000-06-14 |
EP2060630A2 (en) | 2009-05-20 |
US9540696B2 (en) | 2017-01-10 |
JP3981416B2 (ja) | 2007-09-26 |
US20100047809A1 (en) | 2010-02-25 |
WO1998045420A1 (en) | 1998-10-15 |
ES2402947T3 (es) | 2013-05-10 |
EP2060630B1 (en) | 2012-10-24 |
DE69840669D1 (de) | 2009-04-30 |
AU7019498A (en) | 1998-10-30 |
EP1007650B1 (en) | 2009-03-18 |
ES2324503T3 (es) | 2009-08-07 |
US20170073776A1 (en) | 2017-03-16 |
US7632643B2 (en) | 2009-12-15 |
US20140017774A1 (en) | 2014-01-16 |
CA2286304A1 (en) | 1998-10-15 |
CA2286304C (en) | 2007-08-07 |
ATE426018T1 (de) | 2009-04-15 |
US20060099658A1 (en) | 2006-05-11 |
EP2060630A3 (en) | 2010-04-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP3981416B2 (ja) | Pca3タンパク質、pca3遺伝子、及びこれらの用途 | |
AU708746B2 (en) | Compositions for the diagnosis, prevention, and treatment of tumor progression | |
WO2002092629A1 (en) | Polynucleotides and polypeptides linked to cancer and/or tumorigenesis | |
US6780984B2 (en) | Method for prognosing cancer and the proteins involved | |
JP2001509378A (ja) | 哺乳動物におけるダブルマスル化を引き起こすミオスタチン遺伝子の変異 | |
Zhu et al. | Isolation of mouse THP gene promoter and demonstration of its kidney-specific activity in transgenic mice | |
JP2002538789A (ja) | 新規なヒトカリクレイン−様遺伝子 | |
US6372490B1 (en) | Nucleic acid encoding the MDM interacting protein | |
JPH10510143A (ja) | Alk−7(アクチビン様キナーゼ)、セリンスレオニンキナーゼレセプター | |
US6361948B1 (en) | Prognostic compositions for prostate cancer and methods of use thereof | |
US6908750B2 (en) | Gene encoding HM1.24 antigen protein and promoter thereof | |
US7087715B2 (en) | Human paris-1 antigen and nucleic acids: diagnostic and therapeutic uses | |
US6207812B1 (en) | Chondrosarcoma associated genes | |
EP1146053A1 (en) | Meg-4 protein | |
EP1133575A2 (en) | Methods and compositions for diagnosis and treatment of cancer based on the transcription factor ets2 | |
AU775409B2 (en) | Meg-3 protein | |
WO2001014552A1 (fr) | Proteine meg-1 | |
MXPA00001252A (en) | 1-&agr;-HYDROXYLASE MATERIALS AND METHODS | |
JP2008156359A (ja) | Alk−7(アクチビン様キナーゼ)、セリンスレオニンキナーゼレセプター | |
JP2006083168A (ja) | Alk−7(アクチビン様キナーゼ)、セリンスレオニンキナーゼレセプター |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070820 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20071026 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20080109 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20080115 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080418 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20080526 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080919 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20081007 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20081211 |
|
A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20090122 |
|
A912 | Re-examination (zenchi) completed and case transferred to appeal board |
Free format text: JAPANESE INTERMEDIATE CODE: A912 Effective date: 20090213 |