JP2007238503A - Bamboo grass extract-containing hair-growing composition - Google Patents
Bamboo grass extract-containing hair-growing composition Download PDFInfo
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本発明は、繊維芽細胞増殖因子５作用を阻害するササエキス調製品を含有する育毛剤組成物に関する。 The present invention relates to a hair restorer composition containing a Sasa extract preparation that inhibits fibroblast growth factor 5 action.
従来、開発されてきた育毛・養毛剤の多くは、発毛を阻害する過剰皮脂分泌を抑制したり、頭皮などの血行不良を解消したり、あるいは男性ホルモンの作用を抑制することにより発毛を促すことを狙いとしている。他方、マツエキスに見られるように古来より植物由来の発育毛剤が存在しており、その育毛作用の実体は明らかでないが、民間療法として用いられてきた。このように多数の育毛・養毛剤が存在しているが、薄毛・脱毛に対して普遍的に効果を示すものは今のところ存在せず、薄毛・脱毛の種類による効果の個人差も大きい。 Many of the hair growth and hair nourishing agents that have been developed so far promote hair growth by suppressing excessive sebum secretion that inhibits hair growth, eliminating poor blood circulation such as the scalp, or suppressing the action of male hormones. It aims to. On the other hand, as seen in pine extract, plant-derived hair growth agents have existed since ancient times, and the substance of the hair growth action is not clear, but has been used as a folk remedy. As described above, there are a large number of hair-growth / hair-restoring agents, but there are no universally effective for thinning / hair removal so far, and there is a great individual difference in the effect depending on the type of thinning / hair removal.
ところで毛は一定の速さで絶えず成長するのではなく、成長期と休止期を繰返しながら成長する。成長期から休止期までの毛の成長と脱毛のサイクル（毛周期）は、身体部位および人間の場合には毛1本ごとに相違する。かかる毛母細胞の毛周期に影響を与える物質
の一つとして、変換成長因子（ＴＧＦ）βは、毛周期を成長期から退行期、休止期へ移行させる作用を示す。その作用を抑制するアンタゴニストが見出され、新規な育毛成分として提案されている。
By the way, hair does not grow constantly at a constant speed, but grows by repeating a growth period and a rest period. The hair growth and hair loss cycle (hair cycle) from the growth period to the rest period is different for each body part and for each human hair. As one of the substances that affect the hair cycle of such hair matrix cells, transforming growth factor (TGF) β exhibits the action of shifting the hair cycle from the growth phase to the regression phase and the resting phase. An antagonist that suppresses the action has been found and proposed as a novel hair-growth component.
本発明者らは、上記ＴＧＦβとは異なるサイトカイン、繊維芽細胞増殖因子５（以下、「ＦＧＦ−５」ともいう。）の生物活性にも毛周期の制御があることを見出した。ＦＧＦ−５タンパク質は、多彩な生理作用を示す繊維芽細胞増殖因子（ＦＧＦ）ファミリーに属する。本発明者らの研究により、ＦＧＦ−５は、毛の成長を阻害し、毛周期を成長期から退行期へ移行させる作用を有していることが明らかとなった。（非特許文献1〜３参照）
。
The present inventors have found that the biological activity of a cytokine, fibroblast growth factor 5 (hereinafter also referred to as “FGF-5”), which is different from TGFβ, has control of the hair cycle. The FGF-5 protein belongs to the fibroblast growth factor (FGF) family that exhibits various physiological actions. Our study revealed that FGF-5 has the action of inhibiting hair growth and shifting the hair cycle from the growth phase to the regression phase. (See Non-Patent Documents 1 to 3)
.
さらに本発明者らは、植物からＦＧＦ−５活性を阻害する物質を効率よく探索するスクリーニング方法ならびにその育毛作用をインビトロおよびインビボで評価する方法を提案した（特許文献１）。 Furthermore, the present inventors have proposed a screening method for efficiently searching for a substance that inhibits FGF-5 activity from plants, and a method for evaluating the hair-restoring action in vitro and in vivo (Patent Document 1).
このような現状から、高齢化と多ストレスの社会を反映して薄毛・脱毛に対し、普遍的に効果を発揮する育毛・養毛剤のニーズは、依然としてまた今後も高い。育毛成分として、育毛効果を有効に発揮するとともに、安全な天然素材から得られ、しかも製造コストを抑制できる育毛作用物質が望まれている。
本発明者らは、ＦＧＦ−５作用の研究過程で、この作用を阻害する活性を持つ物質が優れた効果を発揮する育毛剤として利用できるとの着想を得て、毛成長に抑制的に働くＦＧ
Ｆ−５作用に対し、阻害作用を有するか、またはアンタゴニストとなる物質を見出すべく、鋭意研究を進めてきた。その結果、本発明者らは特定の植物抽出物がその目的に合致することを見出しており、さらに従来から提供され、使用されているササエキス製品の中で、特定の調製品についてもＦＧＦ−５作用の阻害活性のあることを見出し、本発明を完成した。
In the process of studying FGF-5 action, the present inventors have obtained the idea that a substance having an activity that inhibits this action can be used as a hair-restoring agent that exhibits an excellent effect, and acts to suppress hair growth. FG
In order to find a substance having an inhibitory action or an antagonist to the F-5 action, intensive research has been carried out. As a result, the present inventors have found that a specific plant extract meets the purpose, and among the Sasa extract products that have been provided and used in the past, FGF-5 is also used for specific preparations. The present invention was completed by finding that it has inhibitory activity.
本発明は、そのような特定のササエキス調製品を含有する育毛剤組成物を提供することを目的とする。すなわちＦＧＦ−５による毛周期制御機構に基づく、優れた育毛・養毛効果を発揮し、しかもほとんど副作用のない育毛剤組成物を提供することにある。 An object of this invention is to provide the hair restorer composition containing such a specific Sasa extract preparation. That is, it is to provide a hair-restoring agent composition that exhibits an excellent hair-restoring / hair-restoring effect based on a hair cycle control mechanism by FGF-5 and has almost no side effects.
本発明の育毛剤組成物は、繊維芽細胞増殖因子５の阻害活性を有するササエキス調製品を少なくとも有効成分として含有することを特徴としている組成物である。
前記ササエキス調製品は、ササ原料からササエキスを調製する過程で生成する抽出液を共沸分留した際の残留液である。
The hair restorer composition of the present invention is a composition characterized in that it contains at least a Sasa extract preparation having an inhibitory activity on fibroblast growth factor 5 as an active ingredient.
The said Sasa extract preparation is a residual liquid at the time of carrying out the azeotropic fractionation of the extract produced | generated in the process of preparing a Sasa extract from a Sasa raw material.
前記ササエキスが、チシマザサエキスまたはクマザサエキスであることが好ましい。
前記ササエキス調製品がＫ-３、Ｋ-４、ＮＫ-３およびＮＫ-４からなる群より少なくとも1種選択されることが望ましい。
It is preferable that the Sasa extract is Chishimasa extract or Kumazasa extract.
It is preferable that the Sasa extract preparation is selected from the group consisting of K-3, K-4, NK-3 and NK-4.
本発明の育毛剤において有効成分の一つとして含有されるササエキスの特定調製品には、ＦＧＦ−５作用を阻害する活性があることが示された。そのＦＧＦ−５作用とは毛母細胞において、毛の成長を阻害し毛周期を成長期から退行期へ移行させる作用である。 It was shown that the specific preparation of Sasa extract contained as one of the active ingredients in the hair restorer of the present invention has an activity of inhibiting FGF-5 action. The FGF-5 action is an action that inhibits hair growth and shifts the hair cycle from the growth phase to the regression phase in the hair matrix cells.
本発明の上記抽出物を含有する育毛剤組成物は、毛母細胞増殖に直接的に作用する成分を含有するために、優れた育毛・養毛効果および脱毛予防効果を発揮する。その有効成分が植物からの抽出物であり、古来より食品添加物、医薬成分として使用されているため、安全に使用できてほとんど副作用がない。 Since the hair restorer composition containing the extract of the present invention contains a component that directly acts on the growth of hair matrix cells, it exhibits excellent hair growth / hair restoration effect and hair loss prevention effect. The active ingredient is an extract from a plant, and since it has been used as a food additive and a pharmaceutical ingredient since ancient times, it can be used safely and has almost no side effects.
〔発明の具体的説明〕
育毛とは、「現在ある髪の毛を健康に保ち、抜け毛を防ぎ、強い髪にする」ことである（日本毛髪業協会による育毛の定義）。本発明における育毛作用も基本的にはこのような作用を指し、より具体的には、例えば毛髪の太さ、色艶や光沢が改善され、その作用が顕著な場合の例として、頭皮のすでに脱毛している部分に頭髪が再び見られるようになる（いわゆる発毛）ことなどを含めた、広義の作用をいう。また、本発明の育毛物質とは、以下に述べるような、そのような育毛作用に対する有効成分、またはそうした成分を含有する物質のことである。「ＦＧＦ−５Ｓ様活性」とは、ＦＧＦ−５を阻害するＦＧＦ−５Ｓと同等または類似の阻害作用をいう。
[Detailed Description of the Invention]
Hair growth refers to “maintaining existing hair in a healthy manner, preventing hair loss and making it stronger” (definition of hair growth by the Japan Hair Industry Association). The hair growth action in the present invention basically also refers to such an action. More specifically, for example, when the hair thickness, color gloss and gloss are improved and the action is remarkable, the scalp already has This refers to the action in a broad sense, including the fact that the hair becomes visible again at the hair loss site (so-called hair growth). Further, the hair growth substance of the present invention is an active ingredient for such hair growth action, as described below, or a substance containing such an ingredient. “FGF-5S-like activity” refers to an inhibitory action equivalent to or similar to FGF-5S that inhibits FGF-5.
以下、本発明を、ササエキス調製品、ＦＧＦ−５の阻害、阻害活性によるスクリーニング、育毛剤組成物について詳細に説明する。
ササエキス調製品
「ササエキス調製品」とは、ササエキスとしての各種調製品の総称である。「ササエキス」は、チシマザサ、Sasa kurilensis（Rupr.）またはクマザサ、Sasa veitdin（Carr.
）Rehd. (Gramineae)の葉からエタノール溶液にて抽出して得られるエキスなどを一括し
ていう。チシマザサ、Sasa kurilensis（Rupr.）もクマザサに含めることもある。クマザサは温帯から亜熱帯にかけて生息する笹の一種で、日本でも家の周りなどに自生する植物である。したがって本発明では、チシマザサ、クマザサなどを含め、一括して「ササ」という。
Hereinafter, the present invention will be described in detail for Sasa extract preparations, FGF-5 inhibition, screening by inhibitory activity, and hair restorer compositions.
Sasa extract preparation “Sasa extract preparation” is a general term for various preparations as Sasa extract. "Sasa extract" can be found in Chishimasa, Sasa kurilensis (Rupr.) Or Kumazasa, Sasa veitdin (Carr.
) Rehd. (Gramineae) leaves collectively refers to extracts obtained by extraction with ethanol solution. Chishimasa, Sasa kurilensis (Rupr.) May also be included in Kumasasa. Kumazasa is a type of moth that inhabits the temperate and subtropical regions, and is a plant that grows naturally around homes in Japan. Therefore, in the present invention, the term “sasa” is collectively referred to as “chishimazasa”, “kumazasa”, and the like.
ササは民間薬として広く用いられた伝統あるハーブであり、薬用植物原料として古来より防腐・抗菌の目的で用いられてきた。また食品添加物（香料・着色料）としても認可されている。このようにササは、抗菌、抗ウィルス作用を持つ安全な天然素材として有用である。 Sasa is a traditional herb widely used as a folk medicine and has been used as a medicinal plant material for antiseptic and antibacterial purposes since ancient times. It is also approved as a food additive (fragrance / colorant). Thus, Sasa is useful as a safe natural material having antibacterial and antiviral effects.
本発明の育毛剤組成物は、ササエキスの特定の調製品に繊維芽細胞増殖因子５（ＦＧＦ−５）作用の阻害活性を有することに基づいたものである。ＦＧＦ−５作用の阻害活性は、ＦＧＦ−５Ｓ様活性でもある。ＦＧＦ−５阻害物質と考えられる成分がおそらくこのササエキスの特定調製品中に1種（または複数）含有されることによる。また、市場に供給
されているササエキス製品には、調製工程および/または調製条件が相違するために、多
種類の調製品がある。
The hair restorer composition of the present invention is based on the fact that a specific preparation of Sasa extract has an inhibitory activity on the action of fibroblast growth factor 5 (FGF-5). The inhibitory activity of FGF-5 action is also FGF-5S-like activity. This is probably due to the inclusion of one (or more) ingredients considered to be FGF-5 inhibitors in this particular Sasa extract preparation. In addition, there are many types of preparations for the Sasa extract products that are supplied to the market due to differences in the preparation process and / or preparation conditions.
例えば、チシマザサから、チシマザサエキスを高圧圧搾法により調製する工程を図１に示す。抽出操作を進めるに従い、経てきた工程が相違する各種のエキスが得られる。調製された各エキスのうち、本発明者の探索により上記ＦＧＦ−５阻害活性を有するもの、有しないものとに分かれることが判明した。 For example, FIG. 1 shows a process of preparing Chishimasa extract from Chishimazasa by a high-pressure pressing method. As the extraction operation proceeds, various extracts having different processes can be obtained. Among the prepared extracts, it was found by the search of the present inventors that the extracts were classified into those having the FGF-5 inhibitory activity and those having no FGF-5 inhibitory activity.
繊維芽細胞増殖因子５（ＦＧＦ−５）の阻害
本発明者らの研究により、ＦＧＦ−５は、毛の成長を阻害し、毛周期を成長期から退行期へ移行させる作用を有していることが明らかにされている。一方、毛包の根元にある毛乳頭は、毛母細胞の増殖や分化を調節することにより毛の成長を制御している。退行期になると毛母細胞は消失してしまうが、毛乳頭細胞は活動が低下するもののその数は減らない。次の成長期には、毛乳頭細胞が再び活性化し、その作用によって毛母細胞が新たに増殖・分化し始める。毛乳頭細胞そのものは変わっていないため、前の成長期と同じ太さの毛を作ることができる。したがって、成長期から退行期・休止期に移る引き金の役割を担っているＦＧＦ−５の作用を何らかの方法で抑制すれば、成長期だけが延長される。成長期をこのように長くする物質は、優れた育毛・養毛効果を発揮すると期待される。
Inhibition of fibroblast growth factor 5 (FGF-5) According to the study by the present inventors, FGF-5 has an action of inhibiting hair growth and shifting the hair cycle from the growth phase to the regression phase. It has been made clear. On the other hand, the hair papilla at the base of the hair follicle controls hair growth by regulating the growth and differentiation of hair matrix cells. In the regression phase, the hair matrix cells disappear, but the number of dermal papilla cells decreases but does not decrease. In the next growth phase, the hair papilla cells are activated again, and the hair matrix cells begin to proliferate and differentiate anew due to the action. Since the dermal papilla cell itself has not changed, hair of the same thickness as the previous growth period can be made. Therefore, if the action of FGF-5, which plays the role of a trigger that shifts from the growth period to the regression / rest period, is suppressed by some method, only the growth period is extended. Substances that increase the growth period in this way are expected to exhibit excellent hair-growth and hair-restoring effects.
実際、成長期の毛胞に大量に発現するＦＧＦ−５の短鎖形分子であるＦＧＦ−５Ｓは、ＦＧＦ−５に対しアンタゴニスト活性を示すことにより、成長期から退行期への移行を遅らせ、毛成長を促進することが、本発明者らにより報告された。ＦＧＦ−５Ｓに似たアンタゴニスト活性を持つ1種または複数の物質が、優れた育毛・養毛効果を発揮すると予想
される。
In fact, FGF-5S, which is a short-chain molecule of FGF-5 that is expressed in large amounts in hair follicles during growth, exhibits an antagonist activity against FGF-5, thereby delaying the transition from the growth phase to the regression phase, It has been reported by the present inventors to promote hair growth. One or more substances having antagonist activity similar to FGF-5S are expected to exhibit excellent hair-growth / hair-restoring effects.
ＦＧＦ−５阻害活性を指標とする育毛作用スクリーング
・培養細胞を用いるイン・ビトロ評価
本発明の育毛剤組成物に使用されるササエキス調製品に育毛作用があるかどうかを判別するためにイン・ビトロ評価を行なった。本評価は、スクリーニングの対象であるササエキス調製品が、培養細胞の増殖を促進するＦＧＦ−５作用を阻害するかどうかを測定することを骨子としている。そのイン・ビトロ評価では、ＦＧＦ−５感受性の、すなわちＦＧＦ−５を添加することにより増殖が促進される培養細胞を使用する。例えばFR-Ba/F3細胞が好ましい。
Hair growth screening using FGF-5 inhibitory activity as an index , in vitro evaluation using cultured cells In order to determine whether the Sasa extract preparation used in the hair growth composition of the present invention has a hair growth action, In vitro evaluation was performed. This evaluation is based on measuring whether the Sasa extract preparation to be screened inhibits the FGF-5 action that promotes the growth of cultured cells. The in vitro evaluation uses cultured cells that are FGF-5 sensitive, ie, whose growth is promoted by the addition of FGF-5. For example, FR-Ba / F3 cells are preferred.
具体的な手順として、ＦＧＦ−５に対して感受性のあるFR-Ba/F3細胞を、10％ウシ胎児血清を含む培養液中で培養する。たとえば96穴培養用プレートに播き換え、培養液にＦＧＦ−５、適当に希釈した評価試料（ササエキス調製品）またはベヒクルを添加して培養する。あるいは、上記細胞が培養されているＦＧＦ−５含有培養液に、評価対象の試料を添加することによってもよい。ここで添加する用量としては、使用する細胞、培養条件等により異なるが、培養液中の濃度として１ｎｇ〜１０ｍｇ／ｍｌが好ましく、調製品の用量
は希釈倍率を変えて少なくとも数点設定するのが好ましい。
As a specific procedure, FR-Ba / F3 cells sensitive to FGF-5 are cultured in a culture medium containing 10% fetal bovine serum. For example, it is transferred to a 96-well culture plate, and FGF-5, an appropriately diluted evaluation sample (sasa extract preparation) or vehicle is added to the culture solution and cultured. Alternatively, the sample to be evaluated may be added to the FGF-5-containing culture medium in which the cells are cultured. The dose to be added here varies depending on the cells used, the culture conditions, etc., but the concentration in the culture solution is preferably 1 ng to 10 mg / ml, and the dose of the preparation should be set at least several points by changing the dilution factor. preferable.
一定期間培養した後、該細胞の増殖の程度を測定する。各ウェルに市販のセルカウンティングキットを添加し、一定時間、たとえば1〜24時間、好ましくは２〜８時間、具体的
には３時間培養した後、ＦＧＦ−５依存的なFR-Ba/F3細胞増殖をいずれの濃度で阻害するかどうかを調べてもよい。上記培養細胞の増殖が抑制されれば、添加した試料が、ＦＧＦ−５による細胞増殖の刺激作用を阻害したこととなる。阻害の程度は、最大阻害の50%阻
害濃度で比較できる。たとえば、マイクロプレートリーダーを用いて細胞数を数えることにより細胞増殖を調べることができる。あるいはそれぞれの細胞に細胞増殖の指標となる物質（チミジンなど）を加え、その取り込みを見ることによって効果を判定してもよい。増殖の程度の比較には、被検物質の代わりにベヒクルを含むものを対照とする。そうしたＦＧＦ−５作用を阻害する活性は、ベヒクルを投与した対照群に対する細胞増殖の相対割合（たとえば％）として示される。
After culturing for a certain period, the degree of proliferation of the cells is measured. A commercially available cell counting kit is added to each well and cultured for a certain period of time, for example 1 to 24 hours, preferably 2 to 8 hours, specifically 3 hours, and then FGF-5-dependent FR-Ba / F3 cells. It may be investigated at what concentration growth is inhibited. If the growth of the cultured cells is suppressed, the added sample has inhibited the cell growth stimulating action by FGF-5. The degree of inhibition can be compared at the 50% inhibitory concentration of maximum inhibition. For example, cell proliferation can be examined by counting the number of cells using a microplate reader. Alternatively, the effect may be determined by adding a substance (such as thymidine) that serves as an index of cell proliferation to each cell and observing its uptake. For comparison of the degree of proliferation, a control containing a vehicle instead of the test substance is used as a control. The activity that inhibits such FGF-5 action is expressed as a relative percentage (eg%) of cell proliferation relative to the vehicle-treated control group.
・動物モデルを用いるイン・ビボ評価
スクリーニングにおけるイン・ビボ評価は、動物、好ましくは毛周期を強制同調させたマウスモデルに試料を塗布投与することによる。次いで適切な観察時期に毛包の形態などを測定して、育毛剤成分の候補となり得るササエキス調製品について、その発毛・育毛効果を評価する。
In vivo evaluation using an animal model In vivo evaluation in screening is by applying and administering a sample to an animal, preferably a mouse model in which the hair cycle is forcibly synchronized. Next, the hair follicle morphology and the like are measured at an appropriate observation time, and the hair growth / hair growth effect of the Sasa extract preparation that can be a candidate for the hair growth ingredient component is evaluated.
ササエキス調製品が発毛・育毛効果を有すると判定されるためには、毛周期の成長期を実際に延長するか、あるいは退行期への移行を遅らせることが必要であり、これらは、具体的に次のようにして確認される。塗布する部位は、毛周期の同調が維持される限り特に限定されるものではない。評価方法における操作を容易にする観点からは、動物の背部周辺が好ましい。その用量は１ｎｇ〜１０ｍｇ乾燥重量／個体／１回程度が好ましい。この作業は１回のみでも、数回繰り返してもよい。 In order for a Sasa extract preparation to be determined to have a hair growth / hair growth effect, it is necessary to actually extend the growth period of the hair cycle or delay the transition to the regression stage. It is confirmed as follows. The site | part to apply is not specifically limited as long as the synchronization of a hair cycle is maintained. From the viewpoint of facilitating operation in the evaluation method, the vicinity of the back of the animal is preferable. The dose is preferably about 1 ng to 10 mg dry weight / individual / once. This operation may be repeated only once or several times.
毛周期が休止期にあるマウス背部を抜毛し、成長期を誘導する。成長期の開始からササエキス調製品（たとえば50％エタノール溶液）を、通常は、１日1回塗布する。成長期の
開始から塗布作業を開始し、最終塗布の翌日、マウスの皮膚を採取して薄切切片を作成し、毛包の形態、大きさを比較する。具体的には、光学顕微鏡下にて観察した皮膚切片像は、写真撮影およびコンピュータへの画像データの取り込みを行なって、切片中の毛包の形態および大きさを比較する。
The back of the mouse whose hair cycle is in the resting phase is removed to induce the growth phase. Sasa extract preparations (for example, 50% ethanol solution) are usually applied once a day from the start of growth. The application work is started from the beginning of the growth period, and the day after the final application, the skin of the mouse is collected and sliced slices are prepared, and the shape and size of the hair follicle are compared. Specifically, skin slice images observed under an optical microscope are photographed and image data taken into a computer to compare the shape and size of hair follicles in the slices.
対照群であるベヒクル（たとえば50%エタノール）塗布群では、毛包が短く既に退行期
に移行しているのに対し、当該エキスの塗布群では、毛包が長く成長期が持続しており、毛周期が成長期から退行期へ移行するのを遅延させていることを確認できれば、発毛・育毛効果があると判断できる。
In the vehicle application group (for example, 50% ethanol), which is a control group, the hair follicles are short and have already shifted to the regression stage, whereas in the application group of the extract, the hair follicles are long and the growth period is sustained. If it can be confirmed that the hair cycle delays the transition from the growth phase to the regression phase, it can be determined that there is a hair growth / hair growth effect.
・育毛作用の評価
ササからエキスを抽出する場合、通常、高圧圧搾、ブランチング、加水分解などの処理を行なうことにより、各種のエキス調製品が生成する。そうした処理を経て得られた液体成分は、さらに共沸分留される（図１）。この共沸分留によって留出液と分留後に残った残留液とに分かれる。本発明者は、図1に示されるササエキス調製品それぞれについて、
上記の育毛作用の評価を行った。その結果、共沸分留において生成する残留液にＦＧＦ−５作用を阻害する活性があることを見出した。それ以外のエキス調製品にはそうした活性が認められなかった。したがって、ササ原料からササエキスを調製する過程で生成する抽出液を共沸分留した際の残留液が、育毛剤組成物の有効成分として利用できる。育毛剤組成物に加えられるササエキス調製品は、図１のＫ-３、Ｋ-４、ＮＫ-３およびＮＫ-４からなる群より少なくとも1種選択されることが望ましい。
-Evaluation of hair-growth action When extracting an extract from Sasa, various extract preparations are normally produced | generated by processes, such as high pressure pressing, blanching, and a hydrolysis. The liquid component obtained through such treatment is further azeotropically distilled (FIG. 1). This azeotropic fractionation separates the distillate and the residual liquid remaining after fractionation. For each of the Sasa extract preparations shown in FIG.
The hair growth action was evaluated. As a result, it was found that the residual liquid produced in the azeotropic fractionation has an activity to inhibit the FGF-5 action. Such activity was not observed in other extract preparations. Therefore, the residual liquid when the extract produced in the process of preparing Sasa extract from Sasa raw material is subjected to azeotropic fractionation can be used as an active ingredient of the hair restorer composition. It is desirable that at least one Sasa extract preparation to be added to the hair restoring composition is selected from the group consisting of K-3, K-4, NK-3, and NK-4 in FIG.
ＦＧＦ−５阻害活性があると認定されたササエキス調製品は、そのままでも本発明に係る育毛剤、化粧料に有効成分として含有させることは可能である。保存、育毛剤の形態など、製剤化の要件を考慮すると、さらに減圧濃縮、希釈、ろ過などの処理を適宜行なって用いてもよい。あるいは、抽出した溶液を、濃縮、噴霧乾燥、凍結乾燥などの処理を行なって乾燥物としてもよい。 Sasa extract preparations certified to have FGF-5 inhibitory activity can be incorporated as active ingredients in the hair restorer and cosmetics according to the present invention. In consideration of the requirements for formulation, such as the form of storage and hair restorer, further treatments such as concentration under reduced pressure, dilution, and filtration may be performed as appropriate. Alternatively, the extracted solution may be subjected to a treatment such as concentration, spray drying, or freeze drying to obtain a dried product.
濃縮、乾燥、または水もしくは極性溶媒への再溶解といった操作、あるいはこれらの生理作用を損なわない範囲で、公知の方法により脱色、脱臭、脱塩などの精製処理、カラムクロマトグラフィーなどによる分画処理による精製をさらに行なってもよい。 Operation such as concentration, drying, or re-dissolution in water or a polar solvent, or purification treatment such as decolorization, deodorization, and desalting by known methods, fractionation treatment by column chromatography, etc., as long as these physiological functions are not impaired Further purification by may be performed.
育毛剤組成物
発毛・育毛の生理メカニズムを担う毛乳頭細胞機能とこれを制御する分子の作用に立脚する育毛物質を有効成分に含む育毛・養毛剤でなければ、満足すべき発育毛効果を得られない。かかる観点から完成された本発明の育毛剤組成物は、上記のＦＧＦ−５の作用を阻害するササエキスを1種または2種以上、有効成分として含有する。本発明の育毛剤組成物は、含有する有効成分の作用が発毛の分子的機作に裏づけられており、しかもその成分が天然素材に由来するという特徴を有している。
Hair restorer composition Hair growth and hair growth agents that have a hair growth / restoration agent that contains a hair growth substance based on the function of the dermal papilla cell function and the molecules that control it as the active ingredient. I can't. The hair restorer composition of the present invention completed from this point of view contains one or more types of Sasa extract that inhibits the action of FGF-5 as an active ingredient. The hair restorer composition of the present invention is characterized in that the action of the active ingredient contained therein is supported by the molecular mechanism of hair growth, and that the ingredient is derived from a natural material.
本発明の育毛剤組成物は、有効成分として選択された上記ササエキス調製品、1種また
は2種以上とともに、下記の成分を所定量、常法に従って混合などの操作を施すことによ
り製造することができる。
The hair restorer composition of the present invention can be produced by subjecting the above-mentioned Sasa extract preparation selected as an active ingredient, one or more kinds, to the following components in predetermined amounts and operations such as mixing according to conventional methods. it can.
育毛剤組成物全量に対する上記エキスの添加量は、育毛剤の形態、他の有効成分の有無、添加後の処理などにより異なる。液状の育毛剤全量に対するその量は、エキスの状態で、好適には0.001〜5.0重量％、より好ましくは0.1〜1重量％である。ここでエキスの状態とは、0.001％未満では、所期の発育毛促進効果が得られず、逆に5％を超えると、その効果は頭打ちであるにもかかわらず、コストが上昇するだけである。育毛剤組成物が、乳状、ゼリー状、ペースト状など半固形または流動状の場合には、ササエキス末などを上記上清の量に基づいて換算した割合で添加すればよい。 The amount of the extract added relative to the total amount of the hair restorer composition varies depending on the form of the hair restorer, the presence or absence of other active ingredients, the treatment after addition, and the like. The amount with respect to the total amount of the liquid hair restoring agent is preferably 0.001 to 5.0% by weight, more preferably 0.1 to 1% by weight in the state of the extract. Here, if the extract is less than 0.001%, the desired hair growth promoting effect cannot be obtained. Conversely, if it exceeds 5%, the effect is limited, but the cost only increases. is there. When the hair growth composition is semi-solid or fluid, such as milk, jelly, or paste, Sasa extract powder or the like may be added at a rate converted based on the amount of the supernatant.
本発明に係る育毛剤組成物は、液剤状、乳剤状、ゲル状、クリーム状、軟膏状、フォーム状、ミスト状、ジェル状など、種々の剤型をとり得る。
具体的には育毛剤組成物は、ヘアトニック、ヘアジェル、ヘアクリーム、ヘアトリーメントローション、ヘアフォーム、ヘアミスト、ヘアシャンプー、ヘアリンスなどの形態で提供される。
The hair restorer composition according to the present invention can take various dosage forms such as liquid, emulsion, gel, cream, ointment, foam, mist, and gel.
Specifically, the hair restorer composition is provided in the form of hair tonic, hair gel, hair cream, hair treatment lotion, hair foam, hair mist, hair shampoo, hair rinse and the like.
本発明の育毛剤組成物には、上記必須成分の他に上記抽出物の作用を損なわない範囲で、油性成分、ヒアルロン酸、セラミドなどの保湿剤、α−トコフェロール、アスコルビン酸誘導体などの抗酸化剤、界面活性剤、紫外線吸収剤、局所刺激剤、毛包賦活剤、香料、色素、防菌防黴剤、ｐＨ調整剤、増粘剤、賦形剤、清涼剤などの一般的な育毛剤用添加物を任意に組み合わせて適宜、含有させることができる。さらにキレート化剤などの安定化剤、経皮吸収促進剤なども加えてもよい。 The hair-restoring agent composition of the present invention has an antioxidant such as an oily component, a moisturizing agent such as hyaluronic acid, ceramide, α-tocopherol, an ascorbic acid derivative, etc., as long as the action of the extract is not impaired in addition to the essential components General hair growth agents such as agents, surfactants, UV absorbers, local stimulants, hair follicle activators, fragrances, pigments, antifungal agents, pH adjusters, thickeners, excipients, and refreshing agents Additives can be appropriately contained in any combination. Furthermore, stabilizers such as chelating agents, transdermal absorption enhancers, and the like may be added.
また、頭皮状態の正常化に有効とされる他の育毛促進成分を併用することにより、育毛・養毛効果の相乗作用を図ることもできる。この目的には、常用の血行促進剤、抗脂漏剤、角質溶解剤などがある。 In addition, by combining with other hair growth promoting components that are effective in normalizing the scalp state, a synergistic effect of hair growth and hair restoration effects can be achieved. This purpose includes conventional blood circulation promoters, antiseborrheic agents, keratolytic agents and the like.
本発明の育毛剤組成物は、症状や体質にかかわらず育毛を促進し、著しい抜毛の改善、治療、種々の脱毛症にも著効を発揮する。また適用の主な対象は、ヒトの頭髪であるが、
イヌ、ネコ、カナリヤ、インコといった愛玩動物の毛並み改善などにも使用できる。さらに毛を採取するか、毛皮を利用するメンヨウ、カシミヤ山羊、アルパカ、アンゴラウサギ、ミンク、キツネなどの動物にも適用して発育毛を促進し毛の色つやを良くすることで、毛または毛皮製品の品質を高める一助となる。
The hair restorer composition of the present invention promotes hair growth regardless of symptoms and constitution, and exhibits remarkable effects on marked improvement in hair removal, treatment, and various alopecia. The main target of application is human hair,
It can also be used to improve the fur of pets such as dogs, cats, canaries and parakeets. Furthermore, hair or fur products can be obtained by collecting hair or applying it to animals such as menyou, cashmere goat, alpaca, angora rabbit, mink, fox etc. It helps to improve the quality.
〔実施例〕
以下、本発明について実施例を挙げてさらに説明を加えるが、本発明がこのような実施例にのみに限定されるものではない。なお特にことわらない限り、％は、重量％である。
〔Example〕
EXAMPLES Hereinafter, although an Example is given and this invention is further demonstrated, this invention is not limited only to such an Example. Unless otherwise stated,% is% by weight.
培養細胞を用いる、クマザサエキス中のＦＧＦ−５阻害物質の検出
使用したチシマザサエキス調製品は、図１に示されたＫ-１、Ｋ-２、Ｋ-３（上清）、
Ｋ-４（上清）、Ｋ-４（沈殿抽出上清）、ＮＫ-２、ＮＫ-３（上清）、ＮＫ-４（沈殿抽
出上清）である。
Detection of FGF-5 inhibitory substance in Kumazasa extract using cultured cells The tishima zasa extract preparation used was K-1, K-2, K-3 (supernatant) shown in FIG.
They are K-4 (supernatant), K-4 (precipitation extraction supernatant), NK-2, NK-3 (supernatant), and NK-4 (precipitation extraction supernatant).
ＦＧＦ−５に応答性のあるFR-Ba/F3細胞を、10％ウシ胎児血清を含む培養液中で培養した。96穴培養用プレート（グライナー社製）に、50,000細胞／ウェルで播き換え、培養液に0.3μg/mlのＦＧＦ−５、適当に希釈した被検物質（上記クマザサエキス調製品）およ
びベヒクルである50％エタノールを添加して３日間培養した。その後、各ウェルにセルカウンティングキット（和光純薬製）を添加し、２時間培養した後、マイクロプレートリーダーを用いて細胞増殖を調べた。その結果を表１に示す。IC50値が小さいものほど、ＦＧＦ−５阻害作用が強い。
FR-Ba / F3 cells responsive to FGF-5 were cultured in a culture medium containing 10% fetal bovine serum. A 96-well culture plate (made by Greiner) was seeded at 50,000 cells / well, and 0.3 μg / ml FGF-5, an appropriately diluted test substance (the above Kumazasa extract preparation), and a vehicle were obtained. 50% ethanol was added and cultured for 3 days. Thereafter, a cell counting kit (manufactured by Wako Pure Chemical Industries, Ltd.) was added to each well, and after culturing for 2 hours, cell proliferation was examined using a microplate reader. The results are shown in Table 1. The smaller the IC50 value, the stronger the FGF-5 inhibitory action.
Ｋ-３、Ｋ-４、ＮＫ-３、及びＮＫ-４は、ＦＧＦ−５依存的なFR-Ba/F3細胞増殖をいずれも低濃度で阻害した。これらの結果から、ササエキス調製品Ｋ-３、Ｋ-４、ＮＫ-３、
及びＮＫ-４は、選択的にＦＧＦ−５阻害活性を持つことが示された。
K-3, K-4, NK-3, and NK-4 all inhibited FGF-5-dependent FR-Ba / F3 cell proliferation at low concentrations. From these results, Sasa extract preparations K-3, K-4, NK-3,
And NK-4 was shown to have selective FGF-5 inhibitory activity.
Ｋ-３、Ｋ-４、ＮＫ-３、及びＮＫ-４は、すべて固液混在製品であるが、ＦＧＦ−５阻害活性はこれらの遠心上清部分（Ｋ-３（上清）、Ｋ-４（上清）、及びＮＫ-３（上清）
）、及びエタノール−水混液による抽出後の遠心分離上清部分（ＮＫ-４（沈殿抽出上清
））に存在した。このことは、ＦＧＦ−５阻害活性を持つ成分が水溶性であり、上清部分にすべて溶出し、固体部分には存在していないことを意味する。なおＫ-３（上清）、Ｋ-４（上清）、ＮＫ-３（上清）、及びＮＫ-４（沈殿抽出上清）の阻害活性に大きな差異はないが、Ｋ-３よりはＫ-４の方が、ＮＫ-３よりはＮＫ-４の方がやや強力である傾向が観察された。
K-3, K-4, NK-3, and NK-4 are all solid-liquid mixed products, but the FGF-5 inhibitory activity is a portion of these centrifugal supernatants (K-3 (supernatant), K- 4 (supernatant) and NK-3 (supernatant)
), And the centrifugation supernatant portion (NK-4 (precipitation extract supernatant)) after extraction with an ethanol-water mixture. This means that the component having FGF-5 inhibitory activity is water-soluble, and all of the components are eluted in the supernatant portion and are not present in the solid portion. Although there is no significant difference in the inhibitory activity of K-3 (supernatant), K-4 (supernatant), NK-3 (supernatant), and NK-4 (precipitation extract supernatant), A tendency was observed for K-4 to be slightly stronger in NK-4 than in NK-3.
毛周期同調マウスを用いる塗布実験
毛周期が休止期にある８週齢のC3H/HeN雄性マウス（日本SLC社より購入）の背部を、手指を使って抜毛し、成長期を一斉に誘導した。誘導翌日から１７、１８、１９、または２０日間、実施例１でＦＧＦ−５阻害活性が認められたＫ-３（上清部）10容を50％エタノ
ール溶液90容に溶解した混合液を、１日1回、抜毛した部分の皮膚約2cm2に0.05ml/cm2ず
つ塗布した。対照群のマウスには、50%エタノールを1日1回、同様に塗布した。すなわち
抜毛後１８日、１９日、２０日、２１日飼育の4パターン（パターンごとに、被検物質群
と対照群を設定）で塗布を行なった。
Application experiment using hair cycle synchronized mice The back of 8-week-old C3H / HeN male mice (purchased from Japan SLC) whose hair cycle is in the resting phase was removed using fingers to induce the growth phase all at once. A mixed solution prepared by dissolving 10 parts of K-3 (supernatant part), in which FGF-5 inhibitory activity was observed in Example 1, in 90 parts of 50% ethanol solution for 17, 18, 19, or 20 days after the induction, Once a day, 0.05 ml / cm 2 was applied to about 2 cm 2 of the skin where the hair had been removed. A control group of mice was similarly coated with 50% ethanol once a day. That is, application was performed in four patterns (test substance group and control group were set for each pattern) reared on the 18th, 19th, 20th, and 21st days after hair removal.
最終塗布の翌日、上記マウスをジエチルエーテル麻酔下放血により屠殺して、塗布部位の皮膚を採取した。採取した皮膚は10％ホルマリン水溶液で固定した後、エタノール脱水系列による処理、キシレンによる透徹を行ない、パラフィン包埋した。ミクロトームを用いて、毛包が上部から下部まで完全に見える方向に４μmの皮膚切片を作成し、シランコ
ートしたスライドグラス上で伸展させた。キシレンによる脱パラフィン処理、エタノール親水系列による処理後、ヘマトキシリンおよびエオシンにより、上記切片を染色し、エタノール脱水系列による処理、キシレンによる透徹を行なった後、エンテラン・ニューを用いて封入した。
The day after the final application, the mouse was sacrificed by exsanguination under diethyl ether anesthesia, and the skin at the application site was collected. The collected skin was fixed with a 10% formalin aqueous solution, then treated with an ethanol dehydration series and permeated with xylene, and embedded in paraffin. Using a microtome, a 4 μm skin section was prepared in a direction in which the hair follicle was completely visible from the top to the bottom, and was stretched on a silane-coated slide glass. After deparaffinization with xylene and treatment with ethanol hydrophilic series, the sections were stained with hematoxylin and eosin, treated with ethanol dehydration series and clarified with xylene, and then encapsulated with Enteran New.
皮膚切片を光学顕微鏡下にて観察し、写真撮影、コンピュータへの画像データの取り込みを行なって、切片中の毛包の形態および大きさを比較した。その皮膚切片像を図２-１
および図２-２に示す。各切片像において、毛胞は真皮にある嚢状の毛球部から表皮へ向
かって伸びる構造物である。像の下部には、皮下（脂肪）組織が見られる。
The skin sections were observed under an optical microscope, photographed, and image data was taken into a computer to compare the shape and size of hair follicles in the sections. Fig. 2-1.
And in Figure 2-2. In each section image, the follicle is a structure extending from the sac-like hair bulb in the dermis toward the epidermis. Subcutaneous (fatty) tissue is seen at the bottom of the image.
「成長期」延長効果の判定： 撮影した薄切切片画像中から切断が適切に行われた「適切な毛包」を選び出し、毛包の長さを測定した。「適切な毛包」とは、毛根部分から毛の皮膚表面開孔部まで一様に観察される毛包のことである。 Determination of “growth period” extension effect: “appropriate hair follicles” appropriately cut from selected sliced slice images were selected, and the length of hair follicles was measured. The “appropriate hair follicle” is a hair follicle that is uniformly observed from the root of the hair to the pores on the skin surface of the hair.
切片中の毛包の形態および大きさの観察結果から、対照群であるエタノール（ベヒクル）塗布群（図２-１）では、毛包が短く既に退行期に移行しているのに対して、上記ササ
エキスＫ-３の塗布群（図２-２）では、毛包は長く成長期が持続していた。
From the observation results of the shape and size of the hair follicles in the section, in the ethanol (vehicle) application group (FIG. 2-1), which is the control group, the hair follicles are short and have already shifted to the regression stage, In the Sasa extract K-3 application group (FIG. 2-2), the hair follicles were long and the growth period was maintained.
表２および表３には、対照群およびＫ-３塗布群における、測定した毛包の本数を示す
。
Tables 2 and 3 show the number of hair follicles measured in the control group and the K-3 application group.
図３は、平均の毛包長(mm)により示される成長期延長効果を表す。
対照群であるエタノール（ベヒクル）塗布群では、毛包が次第に短くなって退行期に移行していくのに対して、上記ササエキスＫ-３の塗布群では、１８日連続塗布（抜毛後飼
育１９日間。図中で、Ｄ１９）以降、平均毛包長は対照群より有意に長く維持され、成長期が持続する傾向にあった。これらの結果は、本発明の植物抽出物が、毛周期の成長期を延長しており、明らかに育毛活性を有することが示している。
FIG. 3 represents the growth period prolonging effect indicated by the average hair follicle length (mm).
In the ethanol (vehicle) application group, which is a control group, the hair follicles gradually become shorter and shift to the regression phase, whereas in the Sasa extract K-3 application group, the application was continued for 18 days (breeding after hair removal 19 In the figure, after D19), the average hair follicle length was maintained significantly longer than that of the control group, and the growth period tended to continue. These results indicate that the plant extract of the present invention has an extended hair cycle growth period and clearly has hair growth activity.
図４は、成長期から退行期への移行に伴う、「毛包長縮小」に対する、Ｋ-３の抑制、
すなわち脱毛防止効果を表すグラフである。１７日連続塗布（抜毛後飼育１８日間）における毛包長を100％として、塗布期間中の毛包長をその相対値で示す。上記ササエキスＫ-３の塗布群では、１８日連続塗布（抜毛後飼育１９日間。図中で、Ｄ１９）以降、平均毛包長は対照群より有意に長く維持され、成長期が持続する傾向にあった。
Fig. 4 shows the suppression of K-3 against "hair follicle length reduction" accompanying the transition from the growth phase to the regression phase.
That is, it is a graph showing the hair loss prevention effect. The hair follicle length during the 17-day continuous application (18 days after raising after hair removal) is defined as 100%, and the hair follicle length during the application period is shown as a relative value. In the Sasa extract K-3 application group, after 18 days of continuous application (19 days after hair removal, D19 in the figure), the average hair follicle length is maintained significantly longer than the control group, and the growth period tends to continue. there were.
ヘアートニック剤の調製
エタノール55重量部、ポリオキシエチレン（8）オレイルアルコールエーテル2重量部、精製水36重量部に実施例1のササエキス調製品、Ｋ-３、1重量部を混合配合する。さらに
常法に従い、メントールなどの香料、酢酸トコフェロールなどの抗酸化剤、サリチル酸などの保存剤、着色剤、ｐＨ調整剤を適量配合して、ヘアトニックタイプの育毛剤組成物を形成する。
Preparation of hair art agent 55 parts by weight of ethanol, 2 parts by weight of polyoxyethylene (8) oleyl alcohol ether and 36 parts by weight of purified water are mixed and mixed with the Sasa extract preparation of Example 1, K-3, 1 part by weight. Furthermore, according to a conventional method, a hair tonic type hair restorer composition is formed by blending appropriate amounts of a fragrance such as menthol, an antioxidant such as tocopherol acetate, a preservative such as salicylic acid, a colorant, and a pH adjuster.
Claims (4)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013105417A1 (en) * | 2012-01-13 | 2013-07-18 | 株式会社アドバンジェン | Biochemical evaluation method |
| DE102015116746A1 (en) * | 2015-10-02 | 2017-04-06 | Stefanie Seyda | Hair restorer and method for its preparation and use of the agent for the prophylactic treatment of hair loss |
| JP2020002054A (en) * | 2018-06-28 | 2020-01-09 | 星製薬株式会社 | Aquaporin expression enhancer containing sasa veitchii extract preparation as active ingredient and healing promoter containing the same as well as production method thereof |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0873324A (en) * | 1994-09-06 | 1996-03-19 | Kao Corp | Hair-tonic and hair-growing agent |
| JPH11199449A (en) * | 1998-01-08 | 1999-07-27 | Hiroko Kanamori | Hair growth agent and usage thereof |
| JP2000103723A (en) * | 1998-09-30 | 2000-04-11 | Seikagaku Kogyo Co Ltd | Trichogenous agent |
| JP2000154120A (en) * | 1998-11-19 | 2000-06-06 | Kureha Chem Ind Co Ltd | Hair restoring and growing composition |
| JP2001343383A (en) * | 2000-05-31 | 2001-12-14 | Natl Inst Of Advanced Industrial Science & Technology Meti | Evaluation method of candidate material of hair cultivating agent or hair nourishing agent component, and alopecia model |
| JP2002173416A (en) * | 2000-12-06 | 2002-06-21 | Kao Corp | Hair cosmetic |
| JP2004224750A (en) * | 2003-01-24 | 2004-08-12 | Haba Laboratories Inc | Beauty fluid |
| JP2004323480A (en) * | 2003-04-28 | 2004-11-18 | Lion Corp | Hair growth and hair tonic agent |
| WO2005034894A1 (en) * | 2003-10-09 | 2005-04-21 | Advangen, Inc. | Hair growth stimulant composition |
| JP2005298460A (en) * | 2004-04-16 | 2005-10-27 | Hatsuyo Ohito | Skin care preparation and method for producing the same |
| JP2006241018A (en) * | 2005-03-01 | 2006-09-14 | Hoodo:Kk | Promoting agent of new hair growth, white hair preventing and/or treating agent, antipruritic composition, and wound healing accelerating agent |
| JP2006257067A (en) * | 2005-02-15 | 2006-09-28 | Susumu Itoku | Production method of sasa albo-marginata extract, sasa albo-marginata extract and its use |
| JP2006347955A (en) * | 2005-06-16 | 2006-12-28 | Shiseido Co Ltd | Hair cosmetic |
-
2006
- 2006-03-08 JP JP2006063136A patent/JP4757665B2/en not_active Expired - Fee Related
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0873324A (en) * | 1994-09-06 | 1996-03-19 | Kao Corp | Hair-tonic and hair-growing agent |
| JPH11199449A (en) * | 1998-01-08 | 1999-07-27 | Hiroko Kanamori | Hair growth agent and usage thereof |
| JP2000103723A (en) * | 1998-09-30 | 2000-04-11 | Seikagaku Kogyo Co Ltd | Trichogenous agent |
| JP2000154120A (en) * | 1998-11-19 | 2000-06-06 | Kureha Chem Ind Co Ltd | Hair restoring and growing composition |
| JP2001343383A (en) * | 2000-05-31 | 2001-12-14 | Natl Inst Of Advanced Industrial Science & Technology Meti | Evaluation method of candidate material of hair cultivating agent or hair nourishing agent component, and alopecia model |
| JP2002173416A (en) * | 2000-12-06 | 2002-06-21 | Kao Corp | Hair cosmetic |
| JP2004224750A (en) * | 2003-01-24 | 2004-08-12 | Haba Laboratories Inc | Beauty fluid |
| JP2004323480A (en) * | 2003-04-28 | 2004-11-18 | Lion Corp | Hair growth and hair tonic agent |
| WO2005034894A1 (en) * | 2003-10-09 | 2005-04-21 | Advangen, Inc. | Hair growth stimulant composition |
| JP2005298460A (en) * | 2004-04-16 | 2005-10-27 | Hatsuyo Ohito | Skin care preparation and method for producing the same |
| JP2006257067A (en) * | 2005-02-15 | 2006-09-28 | Susumu Itoku | Production method of sasa albo-marginata extract, sasa albo-marginata extract and its use |
| JP2006241018A (en) * | 2005-03-01 | 2006-09-14 | Hoodo:Kk | Promoting agent of new hair growth, white hair preventing and/or treating agent, antipruritic composition, and wound healing accelerating agent |
| JP2006347955A (en) * | 2005-06-16 | 2006-12-28 | Shiseido Co Ltd | Hair cosmetic |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013105417A1 (en) * | 2012-01-13 | 2013-07-18 | 株式会社アドバンジェン | Biochemical evaluation method |
| JP5342076B1 (en) * | 2012-01-13 | 2013-11-13 | 株式会社アドバンジェン | Biochemical evaluation method |
| DE102015116746A1 (en) * | 2015-10-02 | 2017-04-06 | Stefanie Seyda | Hair restorer and method for its preparation and use of the agent for the prophylactic treatment of hair loss |
| JP2020002054A (en) * | 2018-06-28 | 2020-01-09 | 星製薬株式会社 | Aquaporin expression enhancer containing sasa veitchii extract preparation as active ingredient and healing promoter containing the same as well as production method thereof |
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